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The map label for this gene is rsmF [H]

Identifier: 126698132

GI number: 126698132

Start: 659280

End: 660590

Strand: Direct

Name: rsmF [H]

Synonym: CD0553

Alternate gene names: 126698132

Gene position: 659280-660590 (Clockwise)

Preceding gene: 126698131

Following gene: 126698133

Centisome position: 15.37

GC content: 30.43

Gene sequence:

>1311_bases
TTGACAGAATTACCAAAGGAATTTTTAGAAGATATTGAAGAAATACTAAAAGATGAATATGATGATTTTATAAAAAGTTA
TGAGGAAAGTAAGACTACTGGGCTTAGAATAAATACTTTAAAAATTGACAAGAAAAAATTATTTGATTTGGAGTTGTTTG
ATTTGAATCAAATACCATGGGCAGAAGAAGGTTTTTATTATGATGATAAAATTGACAGACCTGGTAGAAATCCACTGCAT
GAGGCTGGAGCATACTATTTGCAAGAGCCTTCTGCAATGAGTGTAATACCAAAGGCAGATATTAAAGAGGGTGAAAAAGT
TCTCGATATGTGTGCTGCTCCTGGTGGAAAATCTACATATATACTATCTAAGCTAAACGATACAGGTTTATTGGTTTCTA
ATGAAATTAATGCTAGTAGAATTAAGGCTCTAGGGGAAAATTTAGAGCGATTTGGAGCGAGAAATTGTATTATAACAAAC
ACCGATTCTAATAGCTTAAGAAAGACGTTTAATGGGTATTTTGATAAGGTTATTATAGATGCACCTTGTTCTGGGCAAGG
TATGTTTAGGAAGGATGAGGTTGCCATATCAGATTGGAGTTATGCAAAGGTAGTAGAATGTCAATCCATCCAAAAAGAGA
TTATAAGAGATGGATATAAAATGTTAAAGAAGGATGGCATTTTAATTTACTCAACTTGTACTTTTTCAAGGGAGGAAAAT
GAGCATGTTATAAATGAGTTTATCAGTGAATATCCACATGCAGAACTTATTGAAATGGAAAGATTATGGCCTCATAAGAT
AAAGGGAGAAGGTCATTTTGTTGCCAAAATAAAAAAATTAGAAGAAGAAGAATGTAATGTCAAAGAATTAAAAATAAAAA
ATTTAGATAAAGAAATAAAGGATTATAGGGAGTTTGAAAAGAAATTTTTAAAGGTTGATTTTTATAGTAATAAAAAAAAT
AAATTTTATTTAAGAGGAGAAAATTTGTACCTACTTCCTGAAATATATCCAGATACGAAAAAACTAAAAGTATTGAGATA
TGGTTTGCATTTAGGGGTTTTAAAGAAGAATAGATTTGAGCCTTCTCATGCACTATCTCATTACTTAAAAGTTGAAGATG
TAAAAAATGTTGAAAACTTCAGTGTACAGAGTGAATCAATTCTTAAATATTTAAAAGGGGATGTTGTAAATTCTAATGAT
AGTAGAGGATGGGTTTTGGTTTCGGTTGAAGGAATACCTTTAGGTTGGGGAAAAGAATCTTCTGGTGTAATTAAGAATCA
TTATCCTAAAGGTCTTAGAAAAGTATTTTAA

Upstream 100 bases:

>100_bases
TAGGAAAAAGAAGAAGAAATAAAATTTAAAGGAAAGACATAAATAATTAAAGTATAATAAATGACACACAAGTAAAGCAG
ATTGAAGGGGGATTATAGAT

Downstream 100 bases:

>100_bases
ACTATTAGAAAAAAGAAGTATAATATTTATATTGTAGGTTTATGGTAGATTATTTTGCTGTAGATTGACTTAATATTTAA
CACAGAGGAGACGATTTTAG

Product: methylase

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDKKKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLH
EAGAYYLQEPSAMSVIPKADIKEGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDGYKMLKKDGILIYSTCTFSREEN
EHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAKIKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKN
KFYLRGENLYLLPEIYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLKGDVVNSND
SRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF

Sequences:

>Translated_436_residues
MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDKKKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLH
EAGAYYLQEPSAMSVIPKADIKEGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDGYKMLKKDGILIYSTCTFSREEN
EHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAKIKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKN
KFYLRGENLYLLPEIYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLKGDVVNSND
SRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF
>Mature_435_residues
TELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDKKKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHE
AGAYYLQEPSAMSVIPKADIKEGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITNT
DSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDGYKMLKKDGILIYSTCTFSREENE
HVINEFISEYPHAELIEMERLWPHKIKGEGHFVAKIKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNK
FYLRGENLYLLPEIYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLKGDVVNSNDS
RGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=293, Percent_Identity=31.3993174061433, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI76150623, Length=293, Percent_Identity=31.3993174061433, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI11545785, Length=198, Percent_Identity=34.3434343434343, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI32698918, Length=243, Percent_Identity=27.5720164609054, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI39995082, Length=288, Percent_Identity=24.3055555555556, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI301336155, Length=185, Percent_Identity=28.1081081081081, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI270288818, Length=276, Percent_Identity=24.2753623188406, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI8922322, Length=276, Percent_Identity=24.2753623188406, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI23199998, Length=278, Percent_Identity=24.8201438848921, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI270288816, Length=276, Percent_Identity=24.2753623188406, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI40316918, Length=236, Percent_Identity=26.6949152542373, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI87081985, Length=463, Percent_Identity=29.1576673866091, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI2367212, Length=294, Percent_Identity=26.530612244898, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17536757, Length=291, Percent_Identity=29.553264604811, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI25143471, Length=232, Percent_Identity=28.0172413793103, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324268, Length=294, Percent_Identity=30.2721088435374, Blast_Score=146, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6319447, Length=177, Percent_Identity=32.7683615819209, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI22024126, Length=290, Percent_Identity=29.3103448275862, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI21355201, Length=302, Percent_Identity=28.8079470198675, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24668781, Length=191, Percent_Identity=26.1780104712042, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356513, Length=191, Percent_Identity=26.1780104712042, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19920494, Length=155, Percent_Identity=30.9677419354839, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 50452; Mature: 50321

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDKKKLFDLELFDLNQIPW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECHHHHEEEEEECCCCCCC
AEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIKEGEKVLDMCAAPGGKSTY
CCCCCEECCCCCCCCCCCHHHHCCCEECCCCHHEECCCCCHHHHHHHHHHHCCCCCCCEE
ILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITNTDSNSLRKTFNGYFDKVIID
EEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCEEEEE
APCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDGYKMLKKDGILIYSTCTFSREEN
CCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCC
EHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAKIKKLEEEECNVKELKIKNLDKEIK
HHHHHHHHHHCCCHHHHHHHHCCCCEECCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHH
DYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPEIYPDTKKLKVLRYGLHLGVLKKNRFE
HHHHHHHHHEEEEEEECCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHHEEHHCCCCC
PSHALSHYLKVEDVKNVENFSVQSESILKYLKGDVVNSNDSRGWVLVSVEGIPLGWGKES
HHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCEECCCCCCC
SGVIKNHYPKGLRKVF
CCCHHHCCCHHHHHCC
>Mature Secondary Structure 
TELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDKKKLFDLELFDLNQIPW
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECHHHHEEEEEECCCCCCC
AEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIKEGEKVLDMCAAPGGKSTY
CCCCCEECCCCCCCCCCCHHHHCCCEECCCCHHEECCCCCHHHHHHHHHHHCCCCCCCEE
ILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITNTDSNSLRKTFNGYFDKVIID
EEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCEEEEE
APCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDGYKMLKKDGILIYSTCTFSREEN
CCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCC
EHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAKIKKLEEEECNVKELKIKNLDKEIK
HHHHHHHHHHCCCHHHHHHHHCCCCEECCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHH
DYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPEIYPDTKKLKVLRYGLHLGVLKKNRFE
HHHHHHHHHEEEEEEECCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHHEEHHCCCCC
PSHALSHYLKVEDVKNVENFSVQSESILKYLKGDVVNSNDSRGWVLVSVEGIPLGWGKES
HHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCEECCCCCCC
SGVIKNHYPKGLRKVF
CCCHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA