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The map label for this gene is yfkN [H]

Identifier: 126697770

GI number: 126697770

Start: 258653

End: 260044

Strand: Direct

Name: yfkN [H]

Synonym: CD0199

Alternate gene names: 126697770

Gene position: 258653-260044 (Clockwise)

Preceding gene: 126697769

Following gene: 126697772

Centisome position: 6.03

GC content: 28.16

Gene sequence:

>1392_bases
ATGAGAAATAAAATAATTATAGGAATTACTTTTTCACTTGTATTACTAATGTCTGGATGTGCTTCAAAACAAACAGCAGA
AATAAACGTATTAGCAACTACTGATTTACATGGAGTTATACCTTATGAGTTAACTTCTTATGTAAAAGAAGAAAGAAAAA
AAGATAAAAATCTAACTTTAGTAGATGCAGGAGATTTTTTTGATAGTGGCGAAGTGTTTGGTTCTAGCATGGATAGTTAT
TTTAGTGCTAGATATAAAGATGGTGATAATAACACTGAAAGTTACATTGAGACGCCTATTTCAAAAGATATGAAAGAGGT
AGGTTATGATGTTGCAGTTCTTGGGAATCATGAATTTATATCCAACAATAAATTTTATCTAGATAATATGATATCTGATT
TTGAGAAGCAAAACATAAATATTTTATCGGCAAATACGTATAAAAAAAATGGAGATAGTTATACAAAACCATACATAATA
AAAAATATTAATACACCTGAAGGAAACGTAAAACTAGGAATTTTAGGGCTTACTATAAAAGAAGTTGGTGAGAGGAAACA
GTGGAAAGATGGAAAACTTGTTGACTCTAAGTCTTTAGAACTAAAAGACCAAGAGGGTTACAATGGTGAATTGTATATGA
ATGATTTAGTAGAAGATGCTAAAAAGTGGGTTGGTATAATGGAAAAAGAGAAGCCTGACATTATAGTTGCTGTGGCACAT
ACAGGTGAGAAACCTAAGAAACCTAAAAACCCAGGAAATAGAATACAAGACTTAGCTCAACAAGTTGATGGAATTGATGC
TATAGTTGCAGGTCATAATCATGTTCAAATTGAGCAACATGATTATAAAAATAAATCAGGAGAAAATGTTATAGTTACTG
AGCCAGGAAAGCATGGGGAATGTATTTCTAAGATTAATTTTAAATTAGAAAAAAACAATGATAAATGGAATATAGTTGAC
AAATCTAGTAAATTAATTAAATTTGAAAAAAATCAAGAAAGTGATAAATTTGGAGAATTTATTTCTTCAATTAATAAAGT
AAGTAAAGATAAGAAAGAAATAAGATTATCAGAAGTAAAGACTCCTGAATGGGATAAAATATATGTTTTTAAATCTGGAA
CATCTGCAGATGATATATATAAAACAATAGGATATAAATGGAGAAATATATCATCTCCAAAGGGTGATGACATGGTGCAG
ATTGTTGTAATGAATAATAATAAGCCTGTTAGCTATGTATATGGTGAAGCTGAAGTTATGCCAATTAATATAAAATGTGA
TGAATCAGAGTATAAAGATAATGTTGTAACTATTTATCCTAATAAAAATGATAAATTTAAAATTGAAAAAGGTAAACAAG
AATATAGTATTAATTTAAAACATATACAATAG

Upstream 100 bases:

>100_bases
TATAGATGACTTTTGTATATTAATGTATGAATATTGATATTATAGAAGAATTGAGTTGTCAATGATACTAAATCAAATAT
TTATAGAAGTTGGAGGGATT

Downstream 100 bases:

>100_bases
AAACTTTTATCTTATTTAAGTTTAGATTGTACTTAAAATAAAGTGTGTATTATTTTAAAAATTGATGATAATTAAAATCA
CAACATTAAGGAATAGTACA

Product: membrane-associated nucleotidase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MRNKIIIGITFSLVLLMSGCASKQTAEINVLATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSY
FSARYKDGDNNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQNINILSANTYKKNGDSYTKPYII
KNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQEGYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAH
TGEKPKKPKNPGNRIQDLAQQVDGIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVD
KSSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDIYKTIGYKWRNISSPKGDDMVQ
IVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVTIYPNKNDKFKIEKGKQEYSINLKHIQ

Sequences:

>Translated_463_residues
MRNKIIIGITFSLVLLMSGCASKQTAEINVLATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSY
FSARYKDGDNNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQNINILSANTYKKNGDSYTKPYII
KNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQEGYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAH
TGEKPKKPKNPGNRIQDLAQQVDGIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVD
KSSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDIYKTIGYKWRNISSPKGDDMVQ
IVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVTIYPNKNDKFKIEKGKQEYSINLKHIQ
>Mature_463_residues
MRNKIIIGITFSLVLLMSGCASKQTAEINVLATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSY
FSARYKDGDNNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQNINILSANTYKKNGDSYTKPYII
KNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQEGYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAH
TGEKPKKPKNPGNRIQDLAQQVDGIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVD
KSSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDIYKTIGYKWRNISSPKGDDMVQ
IVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVTIYPNKNDKFKIEKGKQEYSINLKHIQ

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=492, Percent_Identity=22.9674796747967, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 52615; Mature: 52615

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00785 5_NUCLEOTIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNKIIIGITFSLVLLMSGCASKQTAEINVLATTDLHGVIPYELTSYVKEERKKDKNLTL
CCCEEEEEHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEE
VDAGDFFDSGEVFGSSMDSYFSARYKDGDNNTESYIETPISKDMKEVGYDVAVLGNHEFI
EECCCCCCCCHHHCCCHHHHHHEEECCCCCCCHHHHHCCHHHHHHHCCCEEEEECCCEEE
SNNKFYLDNMISDFEKQNINILSANTYKKNGDSYTKPYIIKNINTPEGNVKLGILGLTIK
CCCCEEEHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEEHH
EVGERKQWKDGKLVDSKSLELKDQEGYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAH
HHCCCCCCCCCCEECCCCCEEECCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCEEEEEEC
TGEKPKKPKNPGNRIQDLAQQVDGIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGE
CCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCCCCEEEEECCCCCCC
CISKINFKLEKNNDKWNIVDKSSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVK
EEEEEEEEEECCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHEEHHHCC
TPEWDKIYVFKSGTSADDIYKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVM
CCCCCEEEEEECCCCHHHHHHHHCCEEECCCCCCCCCEEEEEEEECCCCEEEEECCEEEE
PINIKCDESEYKDNVVTIYPNKNDKFKIEKGKQEYSINLKHIQ
EEEEEECCHHCCCCEEEEECCCCCEEEEECCCEEEEEEEEEEC
>Mature Secondary Structure
MRNKIIIGITFSLVLLMSGCASKQTAEINVLATTDLHGVIPYELTSYVKEERKKDKNLTL
CCCEEEEEHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEE
VDAGDFFDSGEVFGSSMDSYFSARYKDGDNNTESYIETPISKDMKEVGYDVAVLGNHEFI
EECCCCCCCCHHHCCCHHHHHHEEECCCCCCCHHHHHCCHHHHHHHCCCEEEEECCCEEE
SNNKFYLDNMISDFEKQNINILSANTYKKNGDSYTKPYIIKNINTPEGNVKLGILGLTIK
CCCCEEEHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEEHH
EVGERKQWKDGKLVDSKSLELKDQEGYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAH
HHCCCCCCCCCCEECCCCCEEECCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCEEEEEEC
TGEKPKKPKNPGNRIQDLAQQVDGIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGE
CCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCCCCEEEEECCCCCCC
CISKINFKLEKNNDKWNIVDKSSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVK
EEEEEEEEEECCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHEEHHHCC
TPEWDKIYVFKSGTSADDIYKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVM
CCCCCEEEEEECCCCHHHHHHHHCCEEECCCCCCCCCEEEEEEEECCCCEEEEECCEEEE
PINIKCDESEYKDNVVTIYPNKNDKFKIEKGKQEYSINLKHIQ
EEEEEECCHHCCCCEEEEECCCCCEEEEECCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]