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The map label for this gene is acoA [H]

Identifier: 126697602

GI number: 126697602

Start: 58454

End: 59422

Strand: Direct

Name: acoA [H]

Synonym: CD0036

Alternate gene names: 126697602

Gene position: 58454-59422 (Clockwise)

Preceding gene: 126697601

Following gene: 126697603

Centisome position: 1.36

GC content: 32.71

Gene sequence:

>969_bases
ATGTATATGTCAATATCAAAAGAAACATTGCTTGAAATGTATAAGAGAATGAATCAGGCTAGAAAATTTGAAGAGAAAGT
TAGTTGGTTTTTTGCAAGAGGTATGGTTCATGGAACAACTCACTTATCTGTAGGTCAAGAAGCGTCAAGTGTGGCGGCTG
TAATGGCTTTAGAAAAAGGAGATTTAGTATCATTAACTCATAGAGGACATAGTCAATTTATAGGAATGGGAATAGACCTA
AATAAAATGATGGCTGAACTTATGGGTAAAGAAACTGGATTTTGTAAGGGAAAAGGAGGCTCAATGCATATAGCAGATAT
TGAGTCAGGTAACTTAGGAGCAAATGGAGTTGTAGGTGGAGGTCTTACAATAGCTCCAGGAGCTGCACTTACACAACAGT
ATAAAAAAACAGGCAAAATAGTTTTATGTAGTTTTGGTGATGGAGCATCAAATGAAGGTACTTTTCATGAAGGAATAAAT
TTATCTTCAATATGGAAATTGCCAATAATATTTTACTGTGAGAATAATTTATACGGTATGTCAACAAGCATAAAACGTCA
TATGAATATAGAAAGTATTGCCACTAGAGCAGCTTCTTATGGAATAGAAGGAATATCAATAGATGGATATAACCCAATAG
AAGTATACGAAACAGTACAAAAAGCTGCTGAGAAGTGTAGAAGAGGAGAAGGACCTGTATTAATTGAAAGTAGAACTTAT
AGATGGTTAGGTCATTCAAAATCAGATGCAAATGTATATAGAACAAAAGAAGAAATAGAATCATGGAAAGCAAAAGACCC
TATTGAATTTTTAAAAAATTATCTTATAGAAAATAATCTATCTAATGAAGATGAATTAGATAAGATTCAAGAATTTGCAA
AGCAATCAATAGAAGATGCAGTAGAGTTTGCTCAAAATAGCCCAAATCCAAAAATAGAGTCTTTATTAGAGGATGTATAT
GCAGACTAA

Upstream 100 bases:

>100_bases
GCGAAATTTATAAATAGTTTAGCAATTAATCACAGTTTAGCACTTAAATTATTAGAAGATTGTGACTAATAGAAAGATAG
ATAAAAGTTAACAGGAGGGA

Downstream 100 bases:

>100_bases
GAATGGTAAAGGAGGAAAATAAAAATGAGTACAAGGGAATTAACATATGCACAAGCAATAAAGGAAGCAATGTCAGAAGA
AATGAGAAGAGATGAAAATG

Product: acetoin:2,6-dichlorophenolindophenol oxidoreductase subunit alpha

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MYMSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQEASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDL
NKMMAELMGKETGFCKGKGGSMHIADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGIN
LSSIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQKAAEKCRRGEGPVLIESRTY
RWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIENNLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVY
AD

Sequences:

>Translated_322_residues
MYMSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQEASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDL
NKMMAELMGKETGFCKGKGGSMHIADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGIN
LSSIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQKAAEKCRRGEGPVLIESRTY
RWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIENNLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVY
AD
>Mature_322_residues
MYMSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQEASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDL
NKMMAELMGKETGFCKGKGGSMHIADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGIN
LSSIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQKAAEKCRRGEGPVLIESRTY
RWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIENNLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVY
AD

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=313, Percent_Identity=37.6996805111821, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI4505685, Length=319, Percent_Identity=37.3040752351097, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI291084742, Length=319, Percent_Identity=37.3040752351097, Blast_Score=205, Evalue=4e-53,
Organism=Homo sapiens, GI291084744, Length=326, Percent_Identity=36.5030674846626, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI291084757, Length=319, Percent_Identity=34.1692789968652, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI11386135, Length=320, Percent_Identity=28.75, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI258645172, Length=320, Percent_Identity=28.75, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI32564172, Length=320, Percent_Identity=34.0625, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17536047, Length=320, Percent_Identity=34.0625, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI86563357, Length=320, Percent_Identity=30.3125, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI86563355, Length=320, Percent_Identity=30.3125, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321026, Length=319, Percent_Identity=37.6175548589342, Blast_Score=216, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24639748, Length=323, Percent_Identity=34.0557275541796, Blast_Score=195, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24639744, Length=322, Percent_Identity=34.1614906832298, Blast_Score=188, Evalue=4e-48,
Organism=Drosophila melanogaster, GI28571106, Length=322, Percent_Identity=34.1614906832298, Blast_Score=188, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24639740, Length=322, Percent_Identity=34.1614906832298, Blast_Score=188, Evalue=5e-48,
Organism=Drosophila melanogaster, GI24639746, Length=308, Percent_Identity=34.7402597402597, Blast_Score=181, Evalue=7e-46,
Organism=Drosophila melanogaster, GI21355903, Length=284, Percent_Identity=27.112676056338, Blast_Score=117, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 35666; Mature: 35666

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYMSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQEASSVAAVMALEKG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCEEEECCCHHHHHHHHHEECCC
DLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHIADIESGNLGANGVVGG
CEEEEEECCCCCEEECCCCHHHHHHHHHCCCCCCEECCCCCEEEEECCCCCCCCCCEECC
GLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLSSIWKLPIIFYCENNLYGM
CEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHEECCCEEEEECCCCCCC
STSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQKAAEKCRRGEGPVLIESRTY
HHHHHHHCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCE
RWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIENNLSNEDELDKIQEFAKQSIEDA
EECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VEFAQNSPNPKIESLLEDVYAD
HHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MYMSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQEASSVAAVMALEKG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCEEEECCCHHHHHHHHHEECCC
DLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHIADIESGNLGANGVVGG
CEEEEEECCCCCEEECCCCHHHHHHHHHCCCCCCEECCCCCEEEEECCCCCCCCCCEECC
GLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLSSIWKLPIIFYCENNLYGM
CEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHEECCCEEEEECCCCCCC
STSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQKAAEKCRRGEGPVLIESRTY
HHHHHHHCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCE
RWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIENNLSNEDELDKIQEFAKQSIEDA
EECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VEFAQNSPNPKIESLLEDVYAD
HHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]