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The map label for this gene is menD

Identifier: 126695971

GI number: 126695971

Start: 560238

End: 562001

Strand: Direct

Name: menD

Synonym: P9301_06331

Alternate gene names: 126695971

Gene position: 560238-562001 (Clockwise)

Preceding gene: 126695968

Following gene: 126695972

Centisome position: 34.12

GC content: 29.48

Gene sequence:

>1764_bases
ATGACATCATCTATTGAATGCCAAAATTTTCTTAGAAGTTTACAACTTTTGAATCTTCTTATAAAAATAGGAGTTCAAAA
TTTAATACTATGTCCTGGTAGTAGATCAGCACCTTTAGCAATAGCTGCTGGAGAATTAAATAAATTAGGAATGGTAAATA
TTTTTAATTCAATAGATGAGAGATCAGCGGGATTCCACTCTCTTGGGATTTCTGCCGCATCAGGTAATCTTTCTTTAGTT
ATTACAACTTCTGGGACTGCGGTAAGTAACTTATTGCCAGCAGCAGTTGAGGCAGATCGATCTTGTAAAGGTGTTATATT
TCTTACTGCCGACAGACCCTTAAGATTAAAAGATTGTGGCGCTAACCAAACAGTAAATCAAGAAGATTTTTTGAGTTCAG
TGTGTAGAAGTGTTTTAAGTACAAATCTAAATGGACTTCATGAAACGCAAGAAAATGAAATCTTGAATTTAGTTCGAATT
ACTGAGAAACAAATATCAACCTTCCCTGGTCCTATTCATTTAAATATTCCTATTGATAAGCCTTTAGATATTTCATTTTT
GAATAAAAAAAATGTTTTAGAGGTTTTTAAAAGAATTTATTTAAAGAAACAATATGTCTTTCAAAAAGTTGAAATAAAGT
CTGATAAAAACAAATTCTTAGAAATTTCAAAAATTTTTAATTTAGATGAATCTGGCATTATTTTGGTAGGTCCCTATCAA
GGTTCAGTAAATGACTTATCTTCTTTCAATAAATCTTTAGAACAATTACAAGAAATTACTGGTTGGCCAGTATTTGCTGA
TCCTGTTTCAGGAGTTTATTCTGATTTGAGAGGATTAGTTGTAAATTGGGAATTAGTCTTAAGAAAACATAAGAATCTTA
TTAAATGTAAACAACTTTTAAGGCTTGGTCCTATGTCATCTTCAATTGATTTAGAGAATTTTCTAATAAAGTTTGAAGGT
ATACAAATTCTTATAAAAGAAAAAAATTATAGAAAATTGGACCCTATAAAAAAATCATTTGAATATGATTTTGGGCTATT
AAATTTTACTTCTATATTGCTAGAGGAATTATCATTTAATGAAAAAAACAAAAAGTCTCTTACTCCGTTAGCTCTAGATC
TCATAGAGGAAGGTAAGCAAATTAAAGATATTTTAAAAGACGAAATTATTATTGATAATAAAATTACTGAATATAAGCTT
GCAAATCTTGTTCCAAAGCTTTGGCCAGCTGAACATCCAATAATGCTCTCCGCTAGTAGCCCGATTAGAGATTGGCTTAC
TTTTTCTGAGAATGGTACTTTAACAAGAAATTGTTTCAGCTTTAGAGGAGCTTCTGGAATAGATGGTACTTTATCTCTTG
CATTAGGAATTTCTAGAATTAAAAATCCTTTACTTCTCGTGACTGGGGATTTGGCTTTTGTTCATGATATAAACGGTTGG
CTAATTGAGAATTCAGTCGACATGAATTTAACAATTCTTCTAATAGATAATAATGGTGGAAATATATTTAATCGTATTTA
TAAAAAAAATTTAAAAGAAGATGAATTTAAAAAACTATTTCTTATGCCTAAAGAAATAAACTGGTCAAAACTCTCAGAGA
GCTATCAAGTAAAATTTAAAAGTGTGTCAAATTTTAAAAAATTACGAGAGGCATTCGATTGGAGCATTTCTATCCAGAAA
TCTGTAATAATTAAAGTTGATATTGATCCAGAAAATGAAATTTATGAAAAAAATGCCCTGCTAGAAAAAATAATTGGCAG
TTAA

Upstream 100 bases:

>100_bases
TTTTTTAAATCCCTTAGTATTGAATTTCTTTTTTCTTGAGTTGTAGACATTTTTTATTTAATTCAGTTATGAGGAAAATC
CCAAAGCATTTATGTAAATT

Downstream 100 bases:

>100_bases
ATTCATTTCTTTCTATCTTTAAATAATTAGTATTCATGAAAGTATTACCTGGTAAAACAAGCTTAAATTGGACAGAATAC
AAATCTTATGAGGATATATT

Product: menaquinone biosynthesis protein

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MTSSIECQNFLRSLQLLNLLIKIGVQNLILCPGSRSAPLAIAAGELNKLGMVNIFNSIDERSAGFHSLGISAASGNLSLV
ITTSGTAVSNLLPAAVEADRSCKGVIFLTADRPLRLKDCGANQTVNQEDFLSSVCRSVLSTNLNGLHETQENEILNLVRI
TEKQISTFPGPIHLNIPIDKPLDISFLNKKNVLEVFKRIYLKKQYVFQKVEIKSDKNKFLEISKIFNLDESGIILVGPYQ
GSVNDLSSFNKSLEQLQEITGWPVFADPVSGVYSDLRGLVVNWELVLRKHKNLIKCKQLLRLGPMSSSIDLENFLIKFEG
IQILIKEKNYRKLDPIKKSFEYDFGLLNFTSILLEELSFNEKNKKSLTPLALDLIEEGKQIKDILKDEIIIDNKITEYKL
ANLVPKLWPAEHPIMLSASSPIRDWLTFSENGTLTRNCFSFRGASGIDGTLSLALGISRIKNPLLLVTGDLAFVHDINGW
LIENSVDMNLTILLIDNNGGNIFNRIYKKNLKEDEFKKLFLMPKEINWSKLSESYQVKFKSVSNFKKLREAFDWSISIQK
SVIIKVDIDPENEIYEKNALLEKIIGS

Sequences:

>Translated_587_residues
MTSSIECQNFLRSLQLLNLLIKIGVQNLILCPGSRSAPLAIAAGELNKLGMVNIFNSIDERSAGFHSLGISAASGNLSLV
ITTSGTAVSNLLPAAVEADRSCKGVIFLTADRPLRLKDCGANQTVNQEDFLSSVCRSVLSTNLNGLHETQENEILNLVRI
TEKQISTFPGPIHLNIPIDKPLDISFLNKKNVLEVFKRIYLKKQYVFQKVEIKSDKNKFLEISKIFNLDESGIILVGPYQ
GSVNDLSSFNKSLEQLQEITGWPVFADPVSGVYSDLRGLVVNWELVLRKHKNLIKCKQLLRLGPMSSSIDLENFLIKFEG
IQILIKEKNYRKLDPIKKSFEYDFGLLNFTSILLEELSFNEKNKKSLTPLALDLIEEGKQIKDILKDEIIIDNKITEYKL
ANLVPKLWPAEHPIMLSASSPIRDWLTFSENGTLTRNCFSFRGASGIDGTLSLALGISRIKNPLLLVTGDLAFVHDINGW
LIENSVDMNLTILLIDNNGGNIFNRIYKKNLKEDEFKKLFLMPKEINWSKLSESYQVKFKSVSNFKKLREAFDWSISIQK
SVIIKVDIDPENEIYEKNALLEKIIGS
>Mature_586_residues
TSSIECQNFLRSLQLLNLLIKIGVQNLILCPGSRSAPLAIAAGELNKLGMVNIFNSIDERSAGFHSLGISAASGNLSLVI
TTSGTAVSNLLPAAVEADRSCKGVIFLTADRPLRLKDCGANQTVNQEDFLSSVCRSVLSTNLNGLHETQENEILNLVRIT
EKQISTFPGPIHLNIPIDKPLDISFLNKKNVLEVFKRIYLKKQYVFQKVEIKSDKNKFLEISKIFNLDESGIILVGPYQG
SVNDLSSFNKSLEQLQEITGWPVFADPVSGVYSDLRGLVVNWELVLRKHKNLIKCKQLLRLGPMSSSIDLENFLIKFEGI
QILIKEKNYRKLDPIKKSFEYDFGLLNFTSILLEELSFNEKNKKSLTPLALDLIEEGKQIKDILKDEIIIDNKITEYKLA
NLVPKLWPAEHPIMLSASSPIRDWLTFSENGTLTRNCFSFRGASGIDGTLSLALGISRIKNPLLLVTGDLAFVHDINGWL
IENSVDMNLTILLIDNNGGNIFNRIYKKNLKEDEFKKLFLMPKEINWSKLSESYQVKFKSVSNFKKLREAFDWSISIQKS
VIIKVDIDPENEIYEKNALLEKIIGS

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=577, Percent_Identity=25.1299826689775, Blast_Score=161, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_PROM0 (A3PBY1)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001090857.1
- ProteinModelPortal:   A3PBY1
- STRING:   A3PBY1
- GeneID:   4911120
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_06331
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   CLSK921906
- BioCyc:   PMAR167546:P9301ORF_0648-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 66026; Mature: 65895

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSIECQNFLRSLQLLNLLIKIGVQNLILCPGSRSAPLAIAAGELNKLGMVNIFNSIDE
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHH
RSAGFHSLGISAASGNLSLVITTSGTAVSNLLPAAVEADRSCKGVIFLTADRPLRLKDCG
HCCCCHHCCCEEECCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCC
ANQTVNQEDFLSSVCRSVLSTNLNGLHETQENEILNLVRITEKQISTFPGPIHLNIPIDK
CCCCCCHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
PLDISFLNKKNVLEVFKRIYLKKQYVFQKVEIKSDKNKFLEISKIFNLDESGIILVGPYQ
CCCEEECCCHHHHHHHHHHHHHHHHHHHHEEECCCCHHHEEHHHHHCCCCCCEEEEECCC
GSVNDLSSFNKSLEQLQEITGWPVFADPVSGVYSDLRGLVVNWELVLRKHKNLIKCKQLL
CCHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLGPMSSSIDLENFLIKFEGIQILIKEKNYRKLDPIKKSFEYDFGLLNFTSILLEELSFN
HCCCCCCCCCHHHHEEEECCEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCC
EKNKKSLTPLALDLIEEGKQIKDILKDEIIIDNKITEYKLANLVPKLWPAEHPIMLSASS
CCCCCCCCHHHHHHHHCCHHHHHHHHHCEEECCCCHHHHHHHHHHHHCCCCCCEEEECCC
PIRDWLTFSENGTLTRNCFSFRGASGIDGTLSLALGISRIKNPLLLVTGDLAFVHDINGW
CHHHHEEECCCCCEEEHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCEEEEEECCCE
LIENSVDMNLTILLIDNNGGNIFNRIYKKNLKEDEFKKLFLMPKEINWSKLSESYQVKFK
EEECCCCCEEEEEEEECCCCHHHHHHHHCCCCCCHHHHEEECCCCCCHHHCCCCCEEEEH
SVSNFKKLREAFDWSISIQKSVIIKVDIDPENEIYEKNALLEKIIGS
HHHHHHHHHHHHCCEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSSIECQNFLRSLQLLNLLIKIGVQNLILCPGSRSAPLAIAAGELNKLGMVNIFNSIDE
CCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHH
RSAGFHSLGISAASGNLSLVITTSGTAVSNLLPAAVEADRSCKGVIFLTADRPLRLKDCG
HCCCCHHCCCEEECCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCC
ANQTVNQEDFLSSVCRSVLSTNLNGLHETQENEILNLVRITEKQISTFPGPIHLNIPIDK
CCCCCCHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
PLDISFLNKKNVLEVFKRIYLKKQYVFQKVEIKSDKNKFLEISKIFNLDESGIILVGPYQ
CCCEEECCCHHHHHHHHHHHHHHHHHHHHEEECCCCHHHEEHHHHHCCCCCCEEEEECCC
GSVNDLSSFNKSLEQLQEITGWPVFADPVSGVYSDLRGLVVNWELVLRKHKNLIKCKQLL
CCHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLGPMSSSIDLENFLIKFEGIQILIKEKNYRKLDPIKKSFEYDFGLLNFTSILLEELSFN
HCCCCCCCCCHHHHEEEECCEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCC
EKNKKSLTPLALDLIEEGKQIKDILKDEIIIDNKITEYKLANLVPKLWPAEHPIMLSASS
CCCCCCCCHHHHHHHHCCHHHHHHHHHCEEECCCCHHHHHHHHHHHHCCCCCCEEEECCC
PIRDWLTFSENGTLTRNCFSFRGASGIDGTLSLALGISRIKNPLLLVTGDLAFVHDINGW
CHHHHEEECCCCCEEEHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCEEEEEECCCE
LIENSVDMNLTILLIDNNGGNIFNRIYKKNLKEDEFKKLFLMPKEINWSKLSESYQVKFK
EEECCCCCEEEEEEEECCCCHHHHHHHHCCCCCCHHHHEEECCCCCCHHHCCCCCEEEEH
SVSNFKKLREAFDWSISIQKSVIIKVDIDPENEIYEKNALLEKIIGS
HHHHHHHHHHHHCCEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA