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The map label for this gene is xthA [H]

Identifier: 126695849

GI number: 126695849

Start: 443850

End: 444695

Strand: Reverse

Name: xthA [H]

Synonym: P9301_05111

Alternate gene names: 126695849

Gene position: 444695-443850 (Counterclockwise)

Preceding gene: 126695854

Following gene: 126695848

Centisome position: 27.08

GC content: 31.09

Gene sequence:

>846_bases
TTGTTAATAGCAACTTGGAATGTTAACTCAATAAGAACCAGACTTTCACAAATCATTGATTGGATTAATCAAGTAAATCC
AGATATTCTATGTTTGCAAGAAACAAAAGTGATGGATGATAATTTCCCGGTTGAACCTTTTGAAAAATTAGGTTATTCAT
TAGAGATCTACGGACAAAAATCATACAACGGTGTGGCTATTATTTCAAAAATAAAGCCAGAAAATGTCAAAAAAGGATTC
AACGGTTGTACAGAGTCTAATCAAAATTCCGAAATTTTCCTAGATCAAAAAAGATTAATTTCTGCTGATATTAATGGGAT
AAAAATAATAAATGTCTATGTTCCAAACGGCTCCTCTCTAGATTCTAGTAAGTTCGCCTACAAAATTAATTGGTTAAATT
GTTTATCTTCATTTTTGGATGAACAAGAAAAAAAAGGAGAATTAATTTGTCTAATGGGTGATTTTAATGTTGCTCCATCT
AACTTGGATATTCATGATCCAAAGAAATATGAAGGAGGAATTATGGCATCTGAGATAGAGAGAAATGCACTAAAAAATGT
ATTGAAAAAAAGATTAATAGATTCTTTCAGAATTTTTGAACAAAATACAGGCCATTGGAGTTGGTGGGATTACCGTAATA
ACGGATTTGAATTAAATAAGGGTTGGAGAATAGACCATATATATATCAGCAAAGAACTGACATCAAAACTTAAAAGTTGT
GTCATAGACTGCTCACCAAGAGGTAATTTACGCCCAAGCGATCATGCCCCTGTAATGATAGAACTTAACTTAAACGGTAT
AAATGAAGATTTTTTTGAGGATGAGGATAATTTTTTCGAAATATAA

Upstream 100 bases:

>100_bases
TGCAGGCTTTTGCATACATTATTTTTCATTTTATTTAATTTCAAAATTCTTTAATGTCAAAATTTTCTGCTAACTTAATT
TTACATAATTTGGTAGAAAT

Downstream 100 bases:

>100_bases
AAAAATAAATTTAATCTCTTCTTAAATGCATGGAAACGCTTATTAATAAAGAGTTATCTAAAATAGAATATGTTTTTATA
GAGTTTTCTTTTGAAATTAA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MLIATWNVNSIRTRLSQIIDWINQVNPDILCLQETKVMDDNFPVEPFEKLGYSLEIYGQKSYNGVAIISKIKPENVKKGF
NGCTESNQNSEIFLDQKRLISADINGIKIINVYVPNGSSLDSSKFAYKINWLNCLSSFLDEQEKKGELICLMGDFNVAPS
NLDIHDPKKYEGGIMASEIERNALKNVLKKRLIDSFRIFEQNTGHWSWWDYRNNGFELNKGWRIDHIYISKELTSKLKSC
VIDCSPRGNLRPSDHAPVMIELNLNGINEDFFEDEDNFFEI

Sequences:

>Translated_281_residues
MLIATWNVNSIRTRLSQIIDWINQVNPDILCLQETKVMDDNFPVEPFEKLGYSLEIYGQKSYNGVAIISKIKPENVKKGF
NGCTESNQNSEIFLDQKRLISADINGIKIINVYVPNGSSLDSSKFAYKINWLNCLSSFLDEQEKKGELICLMGDFNVAPS
NLDIHDPKKYEGGIMASEIERNALKNVLKKRLIDSFRIFEQNTGHWSWWDYRNNGFELNKGWRIDHIYISKELTSKLKSC
VIDCSPRGNLRPSDHAPVMIELNLNGINEDFFEDEDNFFEI
>Mature_281_residues
MLIATWNVNSIRTRLSQIIDWINQVNPDILCLQETKVMDDNFPVEPFEKLGYSLEIYGQKSYNGVAIISKIKPENVKKGF
NGCTESNQNSEIFLDQKRLISADINGIKIINVYVPNGSSLDSSKFAYKINWLNCLSSFLDEQEKKGELICLMGDFNVAPS
NLDIHDPKKYEGGIMASEIERNALKNVLKKRLIDSFRIFEQNTGHWSWWDYRNNGFELNKGWRIDHIYISKELTSKLKSC
VIDCSPRGNLRPSDHAPVMIELNLNGINEDFFEDEDNFFEI

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=270, Percent_Identity=31.4814814814815, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI18375503, Length=270, Percent_Identity=31.4814814814815, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI18375501, Length=270, Percent_Identity=31.4814814814815, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=34.9624060150376, Blast_Score=155, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI71989536, Length=266, Percent_Identity=28.1954887218045, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI221330655, Length=270, Percent_Identity=31.1111111111111, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI17136678, Length=270, Percent_Identity=31.1111111111111, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 32373; Mature: 32373

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIATWNVNSIRTRLSQIIDWINQVNPDILCLQETKVMDDNFPVEPFEKLGYSLEIYGQK
CEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCCCHHHHCCCEEEEEECC
SYNGVAIISKIKPENVKKGFNGCTESNQNSEIFLDQKRLISADINGIKIINVYVPNGSSL
CCCCEEEEEECCCHHHHHHCCCCCCCCCCCEEEEEHHHHEECCCCCEEEEEEEECCCCCC
DSSKFAYKINWLNCLSSFLDEQEKKGELICLMGDFNVAPSNLDIHDPKKYEGGIMASEIE
CCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHH
RNALKNVLKKRLIDSFRIFEQNTGHWSWWDYRNNGFELNKGWRIDHIYISKELTSKLKSC
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCEEEEEEEECHHHHHHHHHH
VIDCSPRGNLRPSDHAPVMIELNLNGINEDFFEDEDNFFEI
EEECCCCCCCCCCCCCCEEEEEECCCCCHHHHCCCCCEECC
>Mature Secondary Structure
MLIATWNVNSIRTRLSQIIDWINQVNPDILCLQETKVMDDNFPVEPFEKLGYSLEIYGQK
CEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCCCHHHHCCCEEEEEECC
SYNGVAIISKIKPENVKKGFNGCTESNQNSEIFLDQKRLISADINGIKIINVYVPNGSSL
CCCCEEEEEECCCHHHHHHCCCCCCCCCCCEEEEEHHHHEECCCCCEEEEEEEECCCCCC
DSSKFAYKINWLNCLSSFLDEQEKKGELICLMGDFNVAPSNLDIHDPKKYEGGIMASEIE
CCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHH
RNALKNVLKKRLIDSFRIFEQNTGHWSWWDYRNNGFELNKGWRIDHIYISKELTSKLKSC
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCEEEEEEEECHHHHHHHHHH
VIDCSPRGNLRPSDHAPVMIELNLNGINEDFFEDEDNFFEI
EEECCCCCCCCCCCCCCEEEEEECCCCCHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]