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The map label for this gene is leuC

Identifier: 126695617

GI number: 126695617

Start: 254886

End: 256289

Strand: Reverse

Name: leuC

Synonym: P9301_02791

Alternate gene names: 126695617

Gene position: 256289-254886 (Counterclockwise)

Preceding gene: 126695618

Following gene: 126695616

Centisome position: 15.61

GC content: 36.47

Gene sequence:

>1404_bases
TTGAGTAAAGATACCTTATTTGATAAAGTTTGGGATTTACACAAAGTTTCCAGTCTCCCTGGTGGCTCAGATCAAATTTT
TATTGGTCTTCACCTCATCCATGAAGTAACAAGTCCTCAAGCATTTGGCGCTTTAAAAGACAAAAATTTAAGGGTAAAAT
TCCCTAGTAGGACTGTCGCTACAGTTGATCATATTGTGCCAACGGATAATCAAAGCAGACCTTTTAAAGATAATCTTGCC
GAGCAAATGATTGAAACACTTGAAAAGAACTGCATAGAACATAAGATAAGATTTTTTAATATTGGCAGTGGAAATCAAGG
TATAGTTCATGTAGTAGCTCCAGAATTGGGGCTAACCCAGCCTGGTATGACAATCGCTTGTGGAGATTCTCATACTTCAA
CGCATGGAGCTTTTGGGTCCATTGCTTTTGGGATAGGTACAAGCCAAGTTAGAGATGTTCTTGCTACCCAAACCATAGCC
ATGAACAAATTGAAAGTAAGGCAGATTTGGTGTGAAAATAAATTATCTAATGGGGTTTATGCTAAAGATTTAGTTCTTCA
TATTATCAATAAACTTGGTGTAAAGGCTGGAGTAGGTTTTGCATATGAGTTTGCAGGACCCGCAATCAATTCATTATCAA
TGGAAGAAAGAATGACAATATGCAATATGTCTATTGAAGGTGGGGCGAGATGCGGCTACATAAATCCTGATGAAAAGACT
TTTAGTTACATTAAAAATAAATTATGTGCGCCCAGAAATGAGAATTGGGATGAAGCACTTTTATGGTGGAAATCATTAAA
AAGCGATGCAAATTCTATTTATGATGATGTAACTAAAATTGATGCTTCAAAAGTAGAACCAACCGTAACCTGGGGAATTA
CTCCAGGTCAAAGTGTAGGCATCAACCAAAAAATCCCTCTTTTAGAAGAATTGTCCCCAGATGACCAATTTGTTGCTAAA
GAAGCTTATGAATATATGGGTTTCAAACCAGGACAGTCAATTAAGGATACTCCAATTGATGTTTGTTTCATAGGCAGTTG
TACCAATGGAAGAATAAGTGACTTAAGAGTTGCTGCTAAAGTAGTAAAAGACAAAAAAGTATCTCAGAATATAAAAGCAT
TTGTAGTCCCAGGATCTGAGAAAGTAGCAAAAGAAGCAAAAATAGAAGGACTTGACAAAATTTTCCTTGATGCAGGTTTT
CAATGGAGAGAACCAGGCTGTTCAATGTGTTTAGCAATGAATTCAGATAAGCTAATAGGTAATCAACTTAGTGCAAGTTC
TAGTAATAGAAATTTCAAAGGAAGACAAGGATCCCCAAATGGAAGGACATTATTAATGAGTCCTGCGATGGTTGCAGCTG
CTGCAATATCAGGGAAAGTAACAGACATAAGAAATTTTATGTGA

Upstream 100 bases:

>100_bases
AACCCGAAATAGACTTGAAATTCAAACACTAAGTGTAAATGTGTGTTTAAACAGCCTCAGATTAATCCTATTATCGGATA
GTAAGTAACTTTTTTACAAA

Downstream 100 bases:

>100_bases
AAATGATCAAAAAGTTTCAAGCCCCAGTAGGGCCTATATCAAAAATAGTAGGTAAATCGATAGTATTAATTGGTGATGAC
ATTGATACCGATCGAATTAT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSKDTLFDKVWDLHKVSSLPGGSDQIFIGLHLIHEVTSPQAFGALKDKNLRVKFPSRTVATVDHIVPTDNQSRPFKDNLA
EQMIETLEKNCIEHKIRFFNIGSGNQGIVHVVAPELGLTQPGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLATQTIA
MNKLKVRQIWCENKLSNGVYAKDLVLHIINKLGVKAGVGFAYEFAGPAINSLSMEERMTICNMSIEGGARCGYINPDEKT
FSYIKNKLCAPRNENWDEALLWWKSLKSDANSIYDDVTKIDASKVEPTVTWGITPGQSVGINQKIPLLEELSPDDQFVAK
EAYEYMGFKPGQSIKDTPIDVCFIGSCTNGRISDLRVAAKVVKDKKVSQNIKAFVVPGSEKVAKEAKIEGLDKIFLDAGF
QWREPGCSMCLAMNSDKLIGNQLSASSSNRNFKGRQGSPNGRTLLMSPAMVAAAAISGKVTDIRNFM

Sequences:

>Translated_467_residues
MSKDTLFDKVWDLHKVSSLPGGSDQIFIGLHLIHEVTSPQAFGALKDKNLRVKFPSRTVATVDHIVPTDNQSRPFKDNLA
EQMIETLEKNCIEHKIRFFNIGSGNQGIVHVVAPELGLTQPGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLATQTIA
MNKLKVRQIWCENKLSNGVYAKDLVLHIINKLGVKAGVGFAYEFAGPAINSLSMEERMTICNMSIEGGARCGYINPDEKT
FSYIKNKLCAPRNENWDEALLWWKSLKSDANSIYDDVTKIDASKVEPTVTWGITPGQSVGINQKIPLLEELSPDDQFVAK
EAYEYMGFKPGQSIKDTPIDVCFIGSCTNGRISDLRVAAKVVKDKKVSQNIKAFVVPGSEKVAKEAKIEGLDKIFLDAGF
QWREPGCSMCLAMNSDKLIGNQLSASSSNRNFKGRQGSPNGRTLLMSPAMVAAAAISGKVTDIRNFM
>Mature_466_residues
SKDTLFDKVWDLHKVSSLPGGSDQIFIGLHLIHEVTSPQAFGALKDKNLRVKFPSRTVATVDHIVPTDNQSRPFKDNLAE
QMIETLEKNCIEHKIRFFNIGSGNQGIVHVVAPELGLTQPGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLATQTIAM
NKLKVRQIWCENKLSNGVYAKDLVLHIINKLGVKAGVGFAYEFAGPAINSLSMEERMTICNMSIEGGARCGYINPDEKTF
SYIKNKLCAPRNENWDEALLWWKSLKSDANSIYDDVTKIDASKVEPTVTWGITPGQSVGINQKIPLLEELSPDDQFVAKE
AYEYMGFKPGQSIKDTPIDVCFIGSCTNGRISDLRVAAKVVKDKKVSQNIKAFVVPGSEKVAKEAKIEGLDKIFLDAGFQ
WREPGCSMCLAMNSDKLIGNQLSASSSNRNFKGRQGSPNGRTLLMSPAMVAAAAISGKVTDIRNFM

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=378, Percent_Identity=26.984126984127, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI41352693, Length=371, Percent_Identity=24.5283018867925, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=49.244060475162, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1787531, Length=447, Percent_Identity=25.2796420581655, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081781, Length=364, Percent_Identity=23.6263736263736, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=394, Percent_Identity=28.9340101522843, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32564738, Length=394, Percent_Identity=28.9340101522843, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=320, Percent_Identity=29.6875, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=454, Percent_Identity=25.9911894273128, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=50.1061571125265, Blast_Score=436, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6322261, Length=408, Percent_Identity=27.6960784313726, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320440, Length=444, Percent_Identity=28.6036036036036, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6323335, Length=394, Percent_Identity=26.9035532994924, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161076999, Length=393, Percent_Identity=27.4809160305344, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI281365315, Length=393, Percent_Identity=27.4809160305344, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI17864292, Length=393, Percent_Identity=27.4809160305344, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI28571643, Length=391, Percent_Identity=27.3657289002558, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24645686, Length=373, Percent_Identity=29.4906166219839, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI17137564, Length=376, Percent_Identity=27.6595744680851, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_PROM0 (A3PAX7)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001090503.1
- ProteinModelPortal:   A3PAX7
- SMR:   A3PAX7
- STRING:   A3PAX7
- GeneID:   4912776
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_02791
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   PMAR167546:P9301ORF_0282-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 51096; Mature: 50965

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDTLFDKVWDLHKVSSLPGGSDQIFIGLHLIHEVTSPQAFGALKDKNLRVKFPSRTVA
CCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHCCCHHHCCCCCCCEEEECCCCCCH
TVDHIVPTDNQSRPFKDNLAEQMIETLEKNCIEHKIRFFNIGSGNQGIVHVVAPELGLTQ
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCEEEEEECCCCCCC
PGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLATQTIAMNKLKVRQIWCENKLSNGVY
CCCEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AKDLVLHIINKLGVKAGVGFAYEFAGPAINSLSMEERMTICNMSIEGGARCGYINPDEKT
HHHHHHHHHHHHCHHHCCCEEEECCCCCCCCCCHHHHHEEEEEEECCCCCCCCCCCCHHH
FSYIKNKLCAPRNENWDEALLWWKSLKSDANSIYDDVTKIDASKVEPTVTWGITPGQSVG
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEECCCCCCCCC
INQKIPLLEELSPDDQFVAKEAYEYMGFKPGQSIKDTPIDVCFIGSCTNGRISDLRVAAK
CCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
VVKDKKVSQNIKAFVVPGSEKVAKEAKIEGLDKIFLDAGFQWREPGCSMCLAMNSDKLIG
HHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCC
NQLSASSSNRNFKGRQGSPNGRTLLMSPAMVAAAAISGKVTDIRNFM
CCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure 
SKDTLFDKVWDLHKVSSLPGGSDQIFIGLHLIHEVTSPQAFGALKDKNLRVKFPSRTVA
CCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHCCCHHHCCCCCCCEEEECCCCCCH
TVDHIVPTDNQSRPFKDNLAEQMIETLEKNCIEHKIRFFNIGSGNQGIVHVVAPELGLTQ
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCEEEEEECCCCCCC
PGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLATQTIAMNKLKVRQIWCENKLSNGVY
CCCEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AKDLVLHIINKLGVKAGVGFAYEFAGPAINSLSMEERMTICNMSIEGGARCGYINPDEKT
HHHHHHHHHHHHCHHHCCCEEEECCCCCCCCCCHHHHHEEEEEEECCCCCCCCCCCCHHH
FSYIKNKLCAPRNENWDEALLWWKSLKSDANSIYDDVTKIDASKVEPTVTWGITPGQSVG
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEECCCCCCCCC
INQKIPLLEELSPDDQFVAKEAYEYMGFKPGQSIKDTPIDVCFIGSCTNGRISDLRVAAK
CCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
VVKDKKVSQNIKAFVVPGSEKVAKEAKIEGLDKIFLDAGFQWREPGCSMCLAMNSDKLIG
HHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCC
NQLSASSSNRNFKGRQGSPNGRTLLMSPAMVAAAAISGKVTDIRNFM
CCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA