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The map label for this gene is leuD [H]

Identifier: 126695616

GI number: 126695616

Start: 254245

End: 254883

Strand: Reverse

Name: leuD [H]

Synonym: P9301_02781

Alternate gene names: 126695616

Gene position: 254883-254245 (Counterclockwise)

Preceding gene: 126695617

Following gene: 126695613

Centisome position: 15.52

GC content: 30.05

Gene sequence:

>639_bases
ATGATCAAAAAGTTTCAAGCCCCAGTAGGGCCTATATCAAAAATAGTAGGTAAATCGATAGTATTAATTGGTGATGACAT
TGATACCGATCGAATTATTCCAGCTCGTTTTTTAAAATGTGTAAATTTCGATAATTTAGGACAATCCGTTTTTGAAGATG
ATAGAAAAAATCTTAAAGGTAAACATCCTTTTGATTTGGAGTCGAATAAAGGATCCTCAATACTAATTGTTAATAGTAAT
TTTGGATGCGGATCTAGCAGAGAACATGCACCCCAAGCCCTTCTTAGGTGGGGAATAAGAGCGATAATTGGAGAAAGTTT
TGCAGAAATTTTCTACTGCAATTGTATAGCTATTGGAATACCATGTTTCACACTCCCAAAAAAATTAATTAAAAATATAC
AAGAAAATAAATATATAAATAATTCCTTATGTGAAATAAACATTAATGAATCAACCTTCAAATCTACTTTTTTTAATTTT
GATTTAGAAATAAAGATTTCTTCAAAAAATATGTTTTTGACTGGTGAGTGGGACGCAACCTCAACACTCCTTGCAAATAA
AAAACTAATCGAAAAAAAAATAAATGATCTACCCTACACAAAGTTTAATCAATCCAATGAATTATTCGAAACCATTTAG

Upstream 100 bases:

>100_bases
AAGGATCCCCAAATGGAAGGACATTATTAATGAGTCCTGCGATGGTTGCAGCTGCTGCAATATCAGGGAAAGTAACAGAC
ATAAGAAATTTTATGTGAAA

Downstream 100 bases:

>100_bases
AGAGAAATTTATTCCTCCAAATTGATTATAAGGTTGATAAAAAACCCCTAATTCATATGATCTTTTTTTAAACTTATCGA
AATTTTCGAACTTATAAAAT

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MIKKFQAPVGPISKIVGKSIVLIGDDIDTDRIIPARFLKCVNFDNLGQSVFEDDRKNLKGKHPFDLESNKGSSILIVNSN
FGCGSSREHAPQALLRWGIRAIIGESFAEIFYCNCIAIGIPCFTLPKKLIKNIQENKYINNSLCEININESTFKSTFFNF
DLEIKISSKNMFLTGEWDATSTLLANKKLIEKKINDLPYTKFNQSNELFETI

Sequences:

>Translated_212_residues
MIKKFQAPVGPISKIVGKSIVLIGDDIDTDRIIPARFLKCVNFDNLGQSVFEDDRKNLKGKHPFDLESNKGSSILIVNSN
FGCGSSREHAPQALLRWGIRAIIGESFAEIFYCNCIAIGIPCFTLPKKLIKNIQENKYINNSLCEININESTFKSTFFNF
DLEIKISSKNMFLTGEWDATSTLLANKKLIEKKINDLPYTKFNQSNELFETI
>Mature_212_residues
MIKKFQAPVGPISKIVGKSIVLIGDDIDTDRIIPARFLKCVNFDNLGQSVFEDDRKNLKGKHPFDLESNKGSSILIVNSN
FGCGSSREHAPQALLRWGIRAIIGESFAEIFYCNCIAIGIPCFTLPKKLIKNIQENKYINNSLCEININESTFKSTFFNF
DLEIKISSKNMFLTGEWDATSTLLANKKLIEKKINDLPYTKFNQSNELFETI

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=108, Percent_Identity=42.5925925925926, Blast_Score=86, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321429, Length=137, Percent_Identity=37.2262773722628, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 23951; Mature: 23951

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKFQAPVGPISKIVGKSIVLIGDDIDTDRIIPARFLKCVNFDNLGQSVFEDDRKNLKG
CCCCCCCCCCHHHHHHCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCC
KHPFDLESNKGSSILIVNSNFGCGSSREHAPQALLRWGIRAIIGESFAEIFYCNCIAIGI
CCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
PCFTLPKKLIKNIQENKYINNSLCEININESTFKSTFFNFDLEIKISSKNMFLTGEWDAT
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHEEEEEEEEEECCCCEEEEECCCCH
STLLANKKLIEKKINDLPYTKFNQSNELFETI
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCC
>Mature Secondary Structure
MIKKFQAPVGPISKIVGKSIVLIGDDIDTDRIIPARFLKCVNFDNLGQSVFEDDRKNLKG
CCCCCCCCCCHHHHHHCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCC
KHPFDLESNKGSSILIVNSNFGCGSSREHAPQALLRWGIRAIIGESFAEIFYCNCIAIGI
CCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
PCFTLPKKLIKNIQENKYINNSLCEININESTFKSTFFNFDLEIKISSKNMFLTGEWDAT
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHEEEEEEEEEECCCCEEEEECCCCH
STLLANKKLIEKKINDLPYTKFNQSNELFETI
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]