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The map label for this gene is clpB2 [H]

Identifier: 126695565

GI number: 126695565

Start: 209214

End: 211970

Strand: Reverse

Name: clpB2 [H]

Synonym: P9301_02271

Alternate gene names: 126695565

Gene position: 211970-209214 (Counterclockwise)

Preceding gene: 126695567

Following gene: 126695564

Centisome position: 12.91

GC content: 30.58

Gene sequence:

>2757_bases
ATGAGAGAAACCCTTACATCCAGTCCCGAATTATTTAGCGATATCAGTTGGAACCTTCTTTTATTAGGGGAAGAAACCGC
AAAAAAATGGGATCATAGCGAATTTAATATTGAACACATAATTCATACATTGTTCTCATCAAGTGAATTCTTTGCTTTCA
TTGAAAAATTATCAATCGACCAAGATACAGTTTTAGACATAACAGAAGATTTTTTAGAAGAGACACCAACAAATGAGTCA
GATATTTTTACTATCGGAGAAGATTTAGAAATTTTATTAGATAACGCGAATCAGATTAAAACTCAATGGGGATCGAGATT
AATAGAAATCCCTCATTTACTAATTGCTCTTGGAAGAGATTTAAGAATTGGGAATTATGTTTTTGAAGAAGGAAACCTTT
CAATGGAAAAATTAGAGGAAGAATTAAAGTTTTACCCAAATATTAATCAATCAAAAGATTCTTTTAATTATGGGAATGTA
ATTGAAATAAATAATCAATCTAATTTTGAATCAGATAAAGAAACTTTAGTTAAAGAAGAAAAATTGAAAAAAGCTATTGT
TCCATTACCGAAAAGTGAACTTCAAATTGAAACCAAAAAACAAGTTGGAAAAGATGAAAATGCTCTTTCAATTTATGGAA
AAGATTTAACAGAATCAGCTAAAAAAGGCTTAATGGATCCCATTTTAGGAAGAGAAAATGAGATCAATAATTTAATGAGG
GTACTCTGCAGAAGAAACAAAAATAACCCTATACTCATTGGCAATCCTGGAGTTGGTAAAACCTCAATTGCAAAATTACT
TGCTCAATTAATTGTAGACAAAAAAGTTCCTGATACTTTAAAGGACTTAAAAATTATTTCACTTGACTTAGGTGCATTAG
TTTCTGGGACCAAATTTAGAGGTCAACTAGAAGAAAGACTAAGTTTAATAATACAGGAACTAAATAATCCAAACCAAGGA
ATGATTCTATTTATTGATGAAATTCACTCAATATTAAGTTCTGACAGATCTTCTACCAACATCAGTAATGTTTTAAAACC
TTTACTAGCTGAAGGAGAACTTAGATGTATCGGTACAACAACACCTGAGAAATTTCGTGAAACTATTGAAAAAGATCAGG
CATTAAATAATTGCTTTCAAAAGATAGCTGTTAATGAACCTTCAGTAGAATTAAGCGCAAAAATACTACAAGGTATCAAA
AAGAAATATGAATCACATCATGGCATAAAAATTTCTGAAGAGGCTGTAAACTATTCTGCAAAATTAGCCGATAGATACAT
CAGCGATAAATGTCTCCCTGATAGCGCAATAGATTTAATTGATGAAGCAGCTGCACAGTTGAAAATCGAGTCTAATAATG
TGCCTCAAATCATTCTTCAACAAGAAAACAAACTTAATACAATTGATGAAAAATTGAATAATTTGCAAGGAGAAAATATC
GAAGCTCAAGAAAAACTATTGAATAATAGACAACAATCAGAGGCAAAATTGAACCTTCTTTTAGAAAATTGGAACAATTT
ACGTGAAGAAATGGAGGAATTATCTATTTTAATGAAAGAAGAAGATAAGATAACCAAACAAATTAAAGATAAATCAAATC
GAGAAATTGAAAATGATCTAGATTATTTAGAAAAGCTTGAAGAAGAGTTAAGTGAAATAGAGAATGACATACAAAAAGTT
GAAGAGAACTTTAATAAAATAAAGAAAAATAGAAATTTCCCTTTTAAATATCAAGTTGAACCTGATGATATTGCTGATGT
TGTCTCAAAAATTACAGGTATTCCAATTTCTAAAGTAGTTTCAAATGAGCGTAAGAAATTAGTCAATCTAGAGACAGAAC
TAAGTGAAAAAGTTATTGGACAAGAAAAAGCTATAGAAGCTGTTTCTGCTGCAATTAGAAGAGCTCGCGTTGGCATGAAA
AGTCCTAAAAGACCTATCGGATCTTTTTTATTTATGGGTCCTACAGGCGTTGGTAAAACAGAATTAGCAAAATCTCTTGC
AACAGCTTTATTTGATGAAGAAGACGCACTTTTAAGATTAGACATGAGTGAATATATGGAGAAAAATGCTGTAGCAAGGC
TTTTGGGAGCTCCTCCAGGTTATGTTGGTTATGAAGAGGGAGGTCAATTAACTGAAGCTGTAAGACGTAAACCCTATTCA
GTAATACTTCTTGATGAGATAGAAAAAGCACATTCAGAAGTTTTTAATATCCTTTTACAAGTTTTAGATGAAGGAAGATT
AACGGACTCTCAAGGAAGGACAGTAGACTTCAAAAATACCGTAATCATTATGACAAGCAACCTAGCTGGTAAATCTATAC
TGGAATATTCACAAAAAATTTCTAGAAGTGATGGAAAGTTAGAAAAAGACCAACAAAACTTAGATGACTCCATTAGTAAT
ACATTGTCTTCAATTTTTAGACCTGAATTTTTAAATAGAATTGATGAAGTGGTAAAGTTTAATCCATTATCTATTGATGA
ACTTCAAAAAATAATAATTTTACAAACAGAAGATTTAAAAAACCTGCTACTTGAACAAAAAATAAATATCGCTATAGACA
AAAAAGTTATTAATAAAATTGCAAATGATTCTTACGAACCTGAATATGGTGCTAGGCCACTTAGCAGGGAACTTAGAAGA
CAAATAGAAAATCCCTTGGCTGCAAAACTTTTAGAGGAAAACTTCAAAAACAAAAAAAATATAATAATTAAACTTAACCC
TGCTAAAAAAGATGAGATCATTTTCAAACCTAGCTGA

Upstream 100 bases:

>100_bases
TCTTTGAAATTTCATCTTACAATTTGAAAATTATTGGATGATTATTAATGCATATCCTATTTAGAATATTAATTAATAAT
CAACTGTAGTTTTATAGTTT

Downstream 100 bases:

>100_bases
GGAGTAAATCAATAAGCTAAAATAAAAATTAAAGCGTAAAGCTTAATTTCAAAAAAAATTTTGTGACAAGTCCTACTACT
AATAAAGAACCTGTTAAAAA

Product: putative ATP-dependent Clp protease, Hsp 100, ATP-binding subunit ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 918; Mature: 918

Protein sequence:

>918_residues
MRETLTSSPELFSDISWNLLLLGEETAKKWDHSEFNIEHIIHTLFSSSEFFAFIEKLSIDQDTVLDITEDFLEETPTNES
DIFTIGEDLEILLDNANQIKTQWGSRLIEIPHLLIALGRDLRIGNYVFEEGNLSMEKLEEELKFYPNINQSKDSFNYGNV
IEINNQSNFESDKETLVKEEKLKKAIVPLPKSELQIETKKQVGKDENALSIYGKDLTESAKKGLMDPILGRENEINNLMR
VLCRRNKNNPILIGNPGVGKTSIAKLLAQLIVDKKVPDTLKDLKIISLDLGALVSGTKFRGQLEERLSLIIQELNNPNQG
MILFIDEIHSILSSDRSSTNISNVLKPLLAEGELRCIGTTTPEKFRETIEKDQALNNCFQKIAVNEPSVELSAKILQGIK
KKYESHHGIKISEEAVNYSAKLADRYISDKCLPDSAIDLIDEAAAQLKIESNNVPQIILQQENKLNTIDEKLNNLQGENI
EAQEKLLNNRQQSEAKLNLLLENWNNLREEMEELSILMKEEDKITKQIKDKSNREIENDLDYLEKLEEELSEIENDIQKV
EENFNKIKKNRNFPFKYQVEPDDIADVVSKITGIPISKVVSNERKKLVNLETELSEKVIGQEKAIEAVSAAIRRARVGMK
SPKRPIGSFLFMGPTGVGKTELAKSLATALFDEEDALLRLDMSEYMEKNAVARLLGAPPGYVGYEEGGQLTEAVRRKPYS
VILLDEIEKAHSEVFNILLQVLDEGRLTDSQGRTVDFKNTVIIMTSNLAGKSILEYSQKISRSDGKLEKDQQNLDDSISN
TLSSIFRPEFLNRIDEVVKFNPLSIDELQKIIILQTEDLKNLLLEQKINIAIDKKVINKIANDSYEPEYGARPLSRELRR
QIENPLAAKLLEENFKNKKNIIIKLNPAKKDEIIFKPS

Sequences:

>Translated_918_residues
MRETLTSSPELFSDISWNLLLLGEETAKKWDHSEFNIEHIIHTLFSSSEFFAFIEKLSIDQDTVLDITEDFLEETPTNES
DIFTIGEDLEILLDNANQIKTQWGSRLIEIPHLLIALGRDLRIGNYVFEEGNLSMEKLEEELKFYPNINQSKDSFNYGNV
IEINNQSNFESDKETLVKEEKLKKAIVPLPKSELQIETKKQVGKDENALSIYGKDLTESAKKGLMDPILGRENEINNLMR
VLCRRNKNNPILIGNPGVGKTSIAKLLAQLIVDKKVPDTLKDLKIISLDLGALVSGTKFRGQLEERLSLIIQELNNPNQG
MILFIDEIHSILSSDRSSTNISNVLKPLLAEGELRCIGTTTPEKFRETIEKDQALNNCFQKIAVNEPSVELSAKILQGIK
KKYESHHGIKISEEAVNYSAKLADRYISDKCLPDSAIDLIDEAAAQLKIESNNVPQIILQQENKLNTIDEKLNNLQGENI
EAQEKLLNNRQQSEAKLNLLLENWNNLREEMEELSILMKEEDKITKQIKDKSNREIENDLDYLEKLEEELSEIENDIQKV
EENFNKIKKNRNFPFKYQVEPDDIADVVSKITGIPISKVVSNERKKLVNLETELSEKVIGQEKAIEAVSAAIRRARVGMK
SPKRPIGSFLFMGPTGVGKTELAKSLATALFDEEDALLRLDMSEYMEKNAVARLLGAPPGYVGYEEGGQLTEAVRRKPYS
VILLDEIEKAHSEVFNILLQVLDEGRLTDSQGRTVDFKNTVIIMTSNLAGKSILEYSQKISRSDGKLEKDQQNLDDSISN
TLSSIFRPEFLNRIDEVVKFNPLSIDELQKIIILQTEDLKNLLLEQKINIAIDKKVINKIANDSYEPEYGARPLSRELRR
QIENPLAAKLLEENFKNKKNIIIKLNPAKKDEIIFKPS
>Mature_918_residues
MRETLTSSPELFSDISWNLLLLGEETAKKWDHSEFNIEHIIHTLFSSSEFFAFIEKLSIDQDTVLDITEDFLEETPTNES
DIFTIGEDLEILLDNANQIKTQWGSRLIEIPHLLIALGRDLRIGNYVFEEGNLSMEKLEEELKFYPNINQSKDSFNYGNV
IEINNQSNFESDKETLVKEEKLKKAIVPLPKSELQIETKKQVGKDENALSIYGKDLTESAKKGLMDPILGRENEINNLMR
VLCRRNKNNPILIGNPGVGKTSIAKLLAQLIVDKKVPDTLKDLKIISLDLGALVSGTKFRGQLEERLSLIIQELNNPNQG
MILFIDEIHSILSSDRSSTNISNVLKPLLAEGELRCIGTTTPEKFRETIEKDQALNNCFQKIAVNEPSVELSAKILQGIK
KKYESHHGIKISEEAVNYSAKLADRYISDKCLPDSAIDLIDEAAAQLKIESNNVPQIILQQENKLNTIDEKLNNLQGENI
EAQEKLLNNRQQSEAKLNLLLENWNNLREEMEELSILMKEEDKITKQIKDKSNREIENDLDYLEKLEEELSEIENDIQKV
EENFNKIKKNRNFPFKYQVEPDDIADVVSKITGIPISKVVSNERKKLVNLETELSEKVIGQEKAIEAVSAAIRRARVGMK
SPKRPIGSFLFMGPTGVGKTELAKSLATALFDEEDALLRLDMSEYMEKNAVARLLGAPPGYVGYEEGGQLTEAVRRKPYS
VILLDEIEKAHSEVFNILLQVLDEGRLTDSQGRTVDFKNTVIIMTSNLAGKSILEYSQKISRSDGKLEKDQQNLDDSISN
TLSSIFRPEFLNRIDEVVKFNPLSIDELQKIIILQTEDLKNLLLEQKINIAIDKKVINKIANDSYEPEYGARPLSRELRR
QIENPLAAKLLEENFKNKKNIIIKLNPAKKDEIIFKPS

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=301, Percent_Identity=39.8671096345515, Blast_Score=210, Evalue=4e-54,
Organism=Escherichia coli, GI1788943, Length=714, Percent_Identity=45.2380952380952, Blast_Score=607, Evalue=1e-174,
Organism=Escherichia coli, GI1787109, Length=340, Percent_Identity=42.9411764705882, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6320464, Length=700, Percent_Identity=42.8571428571429, Blast_Score=547, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6323002, Length=930, Percent_Identity=34.1935483870968, Blast_Score=493, Evalue=1e-140,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 104241; Mature: 104241

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRETLTSSPELFSDISWNLLLLGEETAKKWDHSEFNIEHIIHTLFSSSEFFAFIEKLSID
CCCCCCCCCHHHHCCCCCEEEEECHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCC
QDTVLDITEDFLEETPTNESDIFTIGEDLEILLDNANQIKTQWGSRLIEIPHLLIALGRD
CHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
LRIGNYVFEEGNLSMEKLEEELKFYPNINQSKDSFNYGNVIEINNQSNFESDKETLVKEE
CEECCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHH
KLKKAIVPLPKSELQIETKKQVGKDENALSIYGKDLTESAKKGLMDPILGRENEINNLMR
HHHHHHCCCCCHHHEEEHHHHCCCCCCCEEEECCHHHHHHHHCCHHHHCCCCHHHHHHHH
VLCRRNKNNPILIGNPGVGKTSIAKLLAQLIVDKKVPDTLKDLKIISLDLGALVSGTKFR
HHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHCCEEEEEEHHHHHCCCHHH
GQLEERLSLIIQELNNPNQGMILFIDEIHSILSSDRSSTNISNVLKPLLAEGELRCIGTT
HHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEECC
TPEKFRETIEKDQALNNCFQKIAVNEPSVELSAKILQGIKKKYESHHGIKISEEAVNYSA
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHH
KLADRYISDKCLPDSAIDLIDEAAAQLKIESNNVPQIILQQENKLNTIDEKLNNLQGENI
HHHHHHHHCCCCCHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCHHHHHHHHCCCCCCC
EAQEKLLNNRQQSEAKLNLLLENWNNLREEMEELSILMKEEDKITKQIKDKSNREIENDL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
DYLEKLEEELSEIENDIQKVEENFNKIKKNRNFPFKYQVEPDDIADVVSKITGIPISKVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCCCHHHHH
SNERKKLVNLETELSEKVIGQEKAIEAVSAAIRRARVGMKSPKRPIGSFLFMGPTGVGKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHH
ELAKSLATALFDEEDALLRLDMSEYMEKNAVARLLGAPPGYVGYEEGGQLTEAVRRKPYS
HHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCE
VILLDEIEKAHSEVFNILLQVLDEGRLTDSQGRTVDFKNTVIIMTSNLAGKSILEYSQKI
EEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCCCHHHHHHHHHHH
SRSDGKLEKDQQNLDDSISNTLSSIFRPEFLNRIDEVVKFNPLSIDELQKIIILQTEDLK
HCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHEEECHHHH
NLLLEQKINIAIDKKVINKIANDSYEPEYGARPLSRELRRQIENPLAAKLLEENFKNKKN
HHHHHHHHCCEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCC
IIIKLNPAKKDEIIFKPS
EEEEECCCCCCCEEECCC
>Mature Secondary Structure
MRETLTSSPELFSDISWNLLLLGEETAKKWDHSEFNIEHIIHTLFSSSEFFAFIEKLSID
CCCCCCCCCHHHHCCCCCEEEEECHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCC
QDTVLDITEDFLEETPTNESDIFTIGEDLEILLDNANQIKTQWGSRLIEIPHLLIALGRD
CHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
LRIGNYVFEEGNLSMEKLEEELKFYPNINQSKDSFNYGNVIEINNQSNFESDKETLVKEE
CEECCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHH
KLKKAIVPLPKSELQIETKKQVGKDENALSIYGKDLTESAKKGLMDPILGRENEINNLMR
HHHHHHCCCCCHHHEEEHHHHCCCCCCCEEEECCHHHHHHHHCCHHHHCCCCHHHHHHHH
VLCRRNKNNPILIGNPGVGKTSIAKLLAQLIVDKKVPDTLKDLKIISLDLGALVSGTKFR
HHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHCCEEEEEEHHHHHCCCHHH
GQLEERLSLIIQELNNPNQGMILFIDEIHSILSSDRSSTNISNVLKPLLAEGELRCIGTT
HHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEECC
TPEKFRETIEKDQALNNCFQKIAVNEPSVELSAKILQGIKKKYESHHGIKISEEAVNYSA
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHH
KLADRYISDKCLPDSAIDLIDEAAAQLKIESNNVPQIILQQENKLNTIDEKLNNLQGENI
HHHHHHHHCCCCCHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCHHHHHHHHCCCCCCC
EAQEKLLNNRQQSEAKLNLLLENWNNLREEMEELSILMKEEDKITKQIKDKSNREIENDL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
DYLEKLEEELSEIENDIQKVEENFNKIKKNRNFPFKYQVEPDDIADVVSKITGIPISKVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCCCHHHHH
SNERKKLVNLETELSEKVIGQEKAIEAVSAAIRRARVGMKSPKRPIGSFLFMGPTGVGKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHH
ELAKSLATALFDEEDALLRLDMSEYMEKNAVARLLGAPPGYVGYEEGGQLTEAVRRKPYS
HHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCE
VILLDEIEKAHSEVFNILLQVLDEGRLTDSQGRTVDFKNTVIIMTSNLAGKSILEYSQKI
EEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCCCHHHHHHHHHHH
SRSDGKLEKDQQNLDDSISNTLSSIFRPEFLNRIDEVVKFNPLSIDELQKIIILQTEDLK
HCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHEEECHHHH
NLLLEQKINIAIDKKVINKIANDSYEPEYGARPLSRELRRQIENPLAAKLLEENFKNKKN
HHHHHHHHCCEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCC
IIIKLNPAKKDEIIFKPS
EEEEECCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]