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The map label for this gene is phoA [H]

Identifier: 126454088

GI number: 126454088

Start: 367756

End: 369186

Strand: Direct

Name: phoA [H]

Synonym: BURPS1106A_0404

Alternate gene names: 126454088

Gene position: 367756-369186 (Clockwise)

Preceding gene: 126451852

Following gene: 126453494

Centisome position: 9.22

GC content: 69.25

Gene sequence:

>1431_bases
GTGAGACTGGCCGCGCTCCTTGGCGCGAGCGTCTTCGCGGCGGCGGGCTGCGGCAGCGACGAGCCGAAGACGCCGGGCGC
ATCGGACAACGGCGCGCAAACGGGCGCCGCGCGCAACGTCATCTTCTTTCTGGGCGACGGAATGGGGATGACGACGCTCA
CGGCCGCGCGAATCTATGCGCTCGGCGAGGACGGCGCGCTCACGCTCGACACGCTGCCCGAGACCGCGTTCGTGAAGACC
TATTCGAACGATGCGCAGGTGACGGACAGCGCGCCGTCGATGTCCGCATATATGACGGGCGTGAAGACGAACAACGAAGT
GATCTCGATGACGCCCGACACGAAGGCGATCGAGCCGAGCGCGAGTCTCACCGGCAACTGCGGCGCGAACAACGGCAAGC
CGGTGCCGACGCTGCTCGAGATCGCGAAGGCGAAGGGGCTCGCCACCGGTGTCGTGACGACGACCCGCGTCACCCACGCG
ACGCCCGCCGCGACCTACGCGCACGTGTGCCACCGCGACGCCGAGAACGACATCGCCGCGCAGCTCGTGCCGGGCGGCGC
GGGCTACAACGCGGCGCTCGGCGGCGGCGTGGATGTCGTGCTGGGCGGCGGCGCGCAATTCTTCGTGCCGAAGGAAGCGG
GCGGCAAGCGCGCGGACGGCCGCCATCTCGTGAACGAGCTGAAGGCGAAGGGCTACGCGATCGCGCAGAACCGCGACGAG
CTCCTCGCCGCCGATGCGACGAAGCGCGGCAAGCTCGCGGGCCTGTTCGCGTCGAGCCACATGAGCTACGACCTCGACCG
CGGCGCGACGAAAGAGCCGAGCCTCGCCGACATGGCGACGCGCGCGCTCGACGTGCTGCAGAAGAACCCGAACGGCTATT
TCCTGATGGTCGAAGGCGGGCGCATCGATCACGCGCTGCACGACACGAACGCGAAGCGCGCGCTGCAGGATACGGTCGCG
TTCGACAACGCGATCAAGGCGACGCTCGACAAGGTGCGCCAGACCGATCCGGAGTTGAAGAACACGCTCGTCGTCGTGAC
GGCCGACCACGATCACACGCTGGTGCTCAACGGCTACGCGGCGCGCACGGGCAAGACGGAAGCGGGCAAGCCGGGCGTGC
TCGGCGTGCTGCGCAACTATCAGACGGGCGCGGTCGCGAAGGACGCGGACGGCGCGCCGTACACGATCATCGGCTTCGGC
AACGGCGAGAACCGCGTGCAAGGCAGCCGCGCGGGCACGAGCCTCACCGACGCGGTGACGGGCGCGGACGATTATCGCCA
GGAAGCGGTCGTGCGCATGGTCAAGGGCGGCGAGACGCACGGCGGCACCGACGTGTTCCTCGGCGCGATCGGCCGCGGCG
CGGACGGCTTTCACGGCGTGATCGAGAACAACAAGGTGTTCGAGCTCGTGCGCGGCGCGGCGCAGCTCTGA

Upstream 100 bases:

>100_bases
AATGCTTTCAATTCAATTCACATTCGCGTCACATCGCCCCCGTATCGTCCTGGCGTTTTTGAAAGAAATCACACCAACGG
GAAGCCGAAACATGAAGCGA

Downstream 100 bases:

>100_bases
GCGCGGCTTGACGGGAAACAGGGGAATACAGGATGTCGACTATCAAGCGCATCGCGGCGGCGGCGTTCGCCGCGGCGGCC
CTCTCGGGCGGTTTCGGCCA

Product: alkaline phosphatase family protein

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MRLAALLGASVFAAAGCGSDEPKTPGASDNGAQTGAARNVIFFLGDGMGMTTLTAARIYALGEDGALTLDTLPETAFVKT
YSNDAQVTDSAPSMSAYMTGVKTNNEVISMTPDTKAIEPSASLTGNCGANNGKPVPTLLEIAKAKGLATGVVTTTRVTHA
TPAATYAHVCHRDAENDIAAQLVPGGAGYNAALGGGVDVVLGGGAQFFVPKEAGGKRADGRHLVNELKAKGYAIAQNRDE
LLAADATKRGKLAGLFASSHMSYDLDRGATKEPSLADMATRALDVLQKNPNGYFLMVEGGRIDHALHDTNAKRALQDTVA
FDNAIKATLDKVRQTDPELKNTLVVVTADHDHTLVLNGYAARTGKTEAGKPGVLGVLRNYQTGAVAKDADGAPYTIIGFG
NGENRVQGSRAGTSLTDAVTGADDYRQEAVVRMVKGGETHGGTDVFLGAIGRGADGFHGVIENNKVFELVRGAAQL

Sequences:

>Translated_476_residues
MRLAALLGASVFAAAGCGSDEPKTPGASDNGAQTGAARNVIFFLGDGMGMTTLTAARIYALGEDGALTLDTLPETAFVKT
YSNDAQVTDSAPSMSAYMTGVKTNNEVISMTPDTKAIEPSASLTGNCGANNGKPVPTLLEIAKAKGLATGVVTTTRVTHA
TPAATYAHVCHRDAENDIAAQLVPGGAGYNAALGGGVDVVLGGGAQFFVPKEAGGKRADGRHLVNELKAKGYAIAQNRDE
LLAADATKRGKLAGLFASSHMSYDLDRGATKEPSLADMATRALDVLQKNPNGYFLMVEGGRIDHALHDTNAKRALQDTVA
FDNAIKATLDKVRQTDPELKNTLVVVTADHDHTLVLNGYAARTGKTEAGKPGVLGVLRNYQTGAVAKDADGAPYTIIGFG
NGENRVQGSRAGTSLTDAVTGADDYRQEAVVRMVKGGETHGGTDVFLGAIGRGADGFHGVIENNKVFELVRGAAQL
>Mature_476_residues
MRLAALLGASVFAAAGCGSDEPKTPGASDNGAQTGAARNVIFFLGDGMGMTTLTAARIYALGEDGALTLDTLPETAFVKT
YSNDAQVTDSAPSMSAYMTGVKTNNEVISMTPDTKAIEPSASLTGNCGANNGKPVPTLLEIAKAKGLATGVVTTTRVTHA
TPAATYAHVCHRDAENDIAAQLVPGGAGYNAALGGGVDVVLGGGAQFFVPKEAGGKRADGRHLVNELKAKGYAIAQNRDE
LLAADATKRGKLAGLFASSHMSYDLDRGATKEPSLADMATRALDVLQKNPNGYFLMVEGGRIDHALHDTNAKRALQDTVA
FDNAIKATLDKVRQTDPELKNTLVVVTADHDHTLVLNGYAARTGKTEAGKPGVLGVLRNYQTGAVAKDADGAPYTIIGFG
NGENRVQGSRAGTSLTDAVTGADDYRQEAVVRMVKGGETHGGTDVFLGAIGRGADGFHGVIENNKVFELVRGAAQL

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=479, Percent_Identity=37.5782881002088, Blast_Score=255, Evalue=5e-68,
Organism=Homo sapiens, GI116734717, Length=473, Percent_Identity=36.3636363636364, Blast_Score=252, Evalue=5e-67,
Organism=Homo sapiens, GI94721246, Length=479, Percent_Identity=37.160751565762, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI157266292, Length=474, Percent_Identity=36.4978902953587, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI294660770, Length=465, Percent_Identity=35.9139784946237, Blast_Score=242, Evalue=7e-64,
Organism=Homo sapiens, GI294660772, Length=433, Percent_Identity=34.1801385681293, Blast_Score=210, Evalue=2e-54,
Organism=Escherichia coli, GI48994877, Length=410, Percent_Identity=32.6829268292683, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320689, Length=339, Percent_Identity=34.2182890855457, Blast_Score=154, Evalue=4e-38,
Organism=Drosophila melanogaster, GI21355151, Length=470, Percent_Identity=37.2340425531915, Blast_Score=247, Evalue=2e-65,
Organism=Drosophila melanogaster, GI85725200, Length=459, Percent_Identity=35.7298474945534, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI85815833, Length=459, Percent_Identity=35.7298474945534, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24585213, Length=469, Percent_Identity=35.8208955223881, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI21355981, Length=458, Percent_Identity=35.1528384279476, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI21358067, Length=480, Percent_Identity=36.0416666666667, Blast_Score=226, Evalue=4e-59,
Organism=Drosophila melanogaster, GI18859923, Length=465, Percent_Identity=37.4193548387097, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24651554, Length=477, Percent_Identity=36.6876310272537, Blast_Score=221, Evalue=8e-58,
Organism=Drosophila melanogaster, GI19921912, Length=485, Percent_Identity=35.2577319587629, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI21355149, Length=475, Percent_Identity=33.2631578947368, Blast_Score=209, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24657827, Length=477, Percent_Identity=33.7526205450734, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24651556, Length=468, Percent_Identity=35.6837606837607, Blast_Score=201, Evalue=7e-52,
Organism=Drosophila melanogaster, GI24657835, Length=470, Percent_Identity=32.1276595744681, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24657842, Length=470, Percent_Identity=31.063829787234, Blast_Score=187, Evalue=1e-47,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 49217; Mature: 49217

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAALLGASVFAAAGCGSDEPKTPGASDNGAQTGAARNVIFFLGDGMGMTTLTAARIYA
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHEEEEEEEE
LGEDGALTLDTLPETAFVKTYSNDAQVTDSAPSMSAYMTGVKTNNEVISMTPDTKAIEPS
ECCCCCEEEECCCCEEEEEEECCCCEECCCCCCCCHHEEECCCCCEEEEECCCCCCCCCC
ASLTGNCGANNGKPVPTLLEIAKAKGLATGVVTTTRVTHATPAATYAHVCHRDAENDIAA
CCCCCCCCCCCCCCCHHHHHHHHHCCCEECEEEEEEEECCCCHHHHHHHHHCCCCCCCEE
QLVPGGAGYNAALGGGVDVVLGGGAQFFVPKEAGGKRADGRHLVNELKAKGYAIAQNRDE
EEECCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHCCEEEECCCCC
LLAADATKRGKLAGLFASSHMSYDLDRGATKEPSLADMATRALDVLQKNPNGYFLMVEGG
EEECCCCCCCCEEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECC
RIDHALHDTNAKRALQDTVAFDNAIKATLDKVRQTDPELKNTLVVVTADHDHTLVLNGYA
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCEEEEEEECCCCEEEEECEE
ARTGKTEAGKPGVLGVLRNYQTGAVAKDADGAPYTIIGFGNGENRVQGSRAGTSLTDAVT
CCCCCCCCCCCCHHHHHHCCCCCCEEECCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHC
GADDYRQEAVVRMVKGGETHGGTDVFLGAIGRGADGFHGVIENNKVFELVRGAAQL
CCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEECCCHHHHHHHHHHCC
>Mature Secondary Structure
MRLAALLGASVFAAAGCGSDEPKTPGASDNGAQTGAARNVIFFLGDGMGMTTLTAARIYA
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHEEEEEEEE
LGEDGALTLDTLPETAFVKTYSNDAQVTDSAPSMSAYMTGVKTNNEVISMTPDTKAIEPS
ECCCCCEEEECCCCEEEEEEECCCCEECCCCCCCCHHEEECCCCCEEEEECCCCCCCCCC
ASLTGNCGANNGKPVPTLLEIAKAKGLATGVVTTTRVTHATPAATYAHVCHRDAENDIAA
CCCCCCCCCCCCCCCHHHHHHHHHCCCEECEEEEEEEECCCCHHHHHHHHHCCCCCCCEE
QLVPGGAGYNAALGGGVDVVLGGGAQFFVPKEAGGKRADGRHLVNELKAKGYAIAQNRDE
EEECCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHCCEEEECCCCC
LLAADATKRGKLAGLFASSHMSYDLDRGATKEPSLADMATRALDVLQKNPNGYFLMVEGG
EEECCCCCCCCEEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECC
RIDHALHDTNAKRALQDTVAFDNAIKATLDKVRQTDPELKNTLVVVTADHDHTLVLNGYA
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCEEEEEEECCCCEEEEECEE
ARTGKTEAGKPGVLGVLRNYQTGAVAKDADGAPYTIIGFGNGENRVQGSRAGTSLTDAVT
CCCCCCCCCCCCHHHHHHCCCCCCEEECCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHC
GADDYRQEAVVRMVKGGETHGGTDVFLGAIGRGADGFHGVIENNKVFELVRGAAQL
CCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]