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The map label for this gene is cobS [H]

Identifier: 126452810

GI number: 126452810

Start: 1014682

End: 1015440

Strand: Direct

Name: cobS [H]

Synonym: BURPS1106A_1038

Alternate gene names: 126452810

Gene position: 1014682-1015440 (Clockwise)

Preceding gene: 126451756

Following gene: 126451705

Centisome position: 25.44

GC content: 73.25

Gene sequence:

>759_bases
ATGAATCCGCTCGCCGAACTGCGCTACTTCGTCGTCGCGCTCGGGTACTTCACGCGCGTGCCGGTGCCGCGCGCGATCAG
CGGCCAGGCCGTCGATCTCGCGCATGCGGCGCGCTACTTTCCGCTCGTCGGCGTGTGCGTCGGCGCGCTCGCCGCGGCCG
TGCATCTCGCCGCGTCGCGCGTGTGGCCGCCGGGCGTCGCGGCGCTCGCGTCGATCGCGGCGACGCTCGTCGCCACGGGC
GCGCTGCACGAAGACGGCCTCGCCGACAGTTGCGACGCGTTCGGCGGCGGCTACACGCGCGAGGACGTGCTGCGGATCAT
GCGCGATTCGCGCATCGGCACGTTCGGCGCGGCGGCGCTCGTCGTCGCGCTGGCGCTGAAGTGGCAGGCGCTCGCCGCGA
TGCCGCCCGCGCGCGCGGCGTGGACGATGATCGCCGCGCATGCGGCGAGCCGCACGCTTGCCGCGAGCCTGCTTCTCACG
CTCGACTATGCGCGCGCCGAAGGCAAGGCGCAGCCGGTCGCGCACCGGATGCGCGCGGGCGCATTCGCGTTCGCGGCGGC
GCTCGGCCTGCCGTGGCTGTTCTGGCCGGACTGGCGCGCGGGCGCGCTCGCGCTCGCGGCGATGCTCGTGCTGCGCGCCG
GCATCGCGCGTTATCTCGACGCGCGCATCGGCGGCTATACCGGCGATTGTCTCGGCTTCGCGCAGCAGGTGTTCGAGCTG
TCGATCTATCTGGCGATGCTCGGATGGATGTCGTCCTGA

Upstream 100 bases:

>100_bases
CGCGCTCGCGCTGCCGCTCGTGCGCGCGGCGGCGGCGTTCGTCAACGAGATGGCGAGCTTCGAATCGGCGGGCGTGGCGA
ACCGCGATGCGTGACGCGCG

Downstream 100 bases:

>100_bases
TCCGCCATCCGGCGGTCGCGCTCGGCACGGGCGTCTGCTACGGCGCGAGCGACGTGCCGCTCGCGGCCGGCGCGGCGGCG
GGCGCGGCCGGCATTCGGCG

Product: cobalamin synthase

Products: Cobalamin 5'-Phosphate; GMP; Cobalamin [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MNPLAELRYFVVALGYFTRVPVPRAISGQAVDLAHAARYFPLVGVCVGALAAAVHLAASRVWPPGVAALASIAATLVATG
ALHEDGLADSCDAFGGGYTREDVLRIMRDSRIGTFGAAALVVALALKWQALAAMPPARAAWTMIAAHAASRTLAASLLLT
LDYARAEGKAQPVAHRMRAGAFAFAAALGLPWLFWPDWRAGALALAAMLVLRAGIARYLDARIGGYTGDCLGFAQQVFEL
SIYLAMLGWMSS

Sequences:

>Translated_252_residues
MNPLAELRYFVVALGYFTRVPVPRAISGQAVDLAHAARYFPLVGVCVGALAAAVHLAASRVWPPGVAALASIAATLVATG
ALHEDGLADSCDAFGGGYTREDVLRIMRDSRIGTFGAAALVVALALKWQALAAMPPARAAWTMIAAHAASRTLAASLLLT
LDYARAEGKAQPVAHRMRAGAFAFAAALGLPWLFWPDWRAGALALAAMLVLRAGIARYLDARIGGYTGDCLGFAQQVFEL
SIYLAMLGWMSS
>Mature_252_residues
MNPLAELRYFVVALGYFTRVPVPRAISGQAVDLAHAARYFPLVGVCVGALAAAVHLAASRVWPPGVAALASIAATLVATG
ALHEDGLADSCDAFGGGYTREDVLRIMRDSRIGTFGAAALVVALALKWQALAAMPPARAAWTMIAAHAASRTLAASLLLT
LDYARAEGKAQPVAHRMRAGAFAFAAALGLPWLFWPDWRAGALALAAMLVLRAGIARYLDARIGGYTGDCLGFAQQVFEL
SIYLAMLGWMSS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=248, Percent_Identity=35.0806451612903, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 26484; Mature: 26484

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLAELRYFVVALGYFTRVPVPRAISGQAVDLAHAARYFPLVGVCVGALAAAVHLAASR
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VWPPGVAALASIAATLVATGALHEDGLADSCDAFGGGYTREDVLRIMRDSRIGTFGAAAL
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHH
VVALALKWQALAAMPPARAAWTMIAAHAASRTLAASLLLTLDYARAEGKAQPVAHRMRAG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AFAFAAALGLPWLFWPDWRAGALALAAMLVLRAGIARYLDARIGGYTGDCLGFAQQVFEL
HHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
SIYLAMLGWMSS
HHHHHHHHHCCC
>Mature Secondary Structure
MNPLAELRYFVVALGYFTRVPVPRAISGQAVDLAHAARYFPLVGVCVGALAAAVHLAASR
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VWPPGVAALASIAATLVATGALHEDGLADSCDAFGGGYTREDVLRIMRDSRIGTFGAAAL
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHH
VVALALKWQALAAMPPARAAWTMIAAHAASRTLAASLLLTLDYARAEGKAQPVAHRMRAG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AFAFAAALGLPWLFWPDWRAGALALAAMLVLRAGIARYLDARIGGYTGDCLGFAQQVFEL
HHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
SIYLAMLGWMSS
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-Cobinamide; Alpha-Ribazole 5'-Phosphate; Alpha-Ribazole [C]

Specific reaction: GDP-Cobinamide + Alpha-Ribazole 5'-Phosphate (or Alpha-Ribazole) = Cobalamin 5'-Phosphate (or GDP-Cobalamin) + GMP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA