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The map label for this gene is rspA [H]

Identifier: 126447832

GI number: 126447832

Start: 1587071

End: 1588279

Strand: Direct

Name: rspA [H]

Synonym: BMA10247_A1648

Alternate gene names: 126447832

Gene position: 1587071-1588279 (Clockwise)

Preceding gene: 126445735

Following gene: 126447385

Centisome position: 67.46

GC content: 68.32

Gene sequence:

>1209_bases
GTGAAAATCGAACGGCTGAACATCATCGTCACCTGCCCCGGCCGGAATTTCGTGACGGTCAAGATCGTCACCGACGAAGG
CGTCCACGGCCTCGGCGACGCGACGCTCAACGGGCGCGAGCTCGCGGTGCGCGCGTATCTGGAGGATCACGTGTTCCCGT
GCCTGATCGGCCGGGACCCGCGCGACATCGAGGACATCTGGCAATACCTGTATCGCGGCGCGTACTGGCGGCGCGGGCCC
GTGACGATGACGGCGATCGCCGCGATCGACATGGCGCTCTGGGACATCAAGAGCAAGCTCGCCGGCATGCCCGTCTATCA
GTTGCTCGGCGGCAAGAGCCGCGAAGGGCTGATGGTCTATGGCCACGCGAACGGGCGCGATCACGACGAGGCGATCGACG
CCGCGCACCGGCACATCGCCGCGGGCTACCGCGCGATCCGCGTGCAGTCGGGCGTGCCGGGGCTGGCGAAGGTGTACGGC
GTCGGCAAGACGGCGGGCGCGTACGAGCCCGCCGAAAAAGGCCTGCCGCCCGAGGAGCCGTGGGACACCGCGCTCTACCT
GCGCCATACGCCCGAGCTGTTCCGCAAGGTGCGCGACGCGCTCGGCGACGCGCCGCACCTGCTGCACGACGCGCATCACC
GGCTCACGCCGATCGAGGCGGCGCGCCTCGGCCGCGATCTCGAGCCGTACCGGCTGTTCTGGCTCGAGGACGCGACGCCC
GCCGAGAACCAGGACGCATTCCGGCTGATCCGCAGCCACACGACGACGACGCTCGCGGTCGGCGAGGTCTTCAATTCGAT
CTGGGATTGCAAGGACCTGATCCGCGATCAGCTGATCGACTACATCCGCGCGACGATCGTGCACGCGGGCGGCATCACGC
ACGTGCGCCGCATCGCCGATTACGCGGCGATGTACCAGGTCCGCACGGGCTTTCACGGCGCGACCGACCTGTCGCCCGTG
TGCATGGCGGCCGCGGTGAACTTCGGACTGTGGGCGCCGAACTTCGGCATCCAGGAGCTGATGCCGCACACGGCGCTGAC
CGACGAGGTGTTCCCGCACAACTATCGCTTCGACGACGGCTATCTCGTGATGGACGACGTGCCCGGCCTCGGCGTCGACA
TCGACGAGACGCTCGCCGCGAACTATCCGTACGAGCGCGCCTACCTGCCCGTCGCCCGCCTGCGCGACGGCTCGATGTGG
AACTGGTGA

Upstream 100 bases:

>100_bases
GGTCGCGGTGCACGTCGACCGCGTGAACGCCCACTTGCCCGAGGTGCGCGAGCGCGCGCCCCACTACTTTGTCGACTGAC
CCCCTCACCGGAGAAACCGC

Downstream 100 bases:

>100_bases
TCGCGCGCCGCCCCACCGTCTAGACGAAACACGAAGGAGAATCAGGACATGCTGAGCGTCGTCGTCGACCAGCCGCATAG
CATGGGCGTGCGCGAAATGC

Product: starvation sensing protein RspA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKIERLNIIVTCPGRNFVTVKIVTDEGVHGLGDATLNGRELAVRAYLEDHVFPCLIGRDPRDIEDIWQYLYRGAYWRRGP
VTMTAIAAIDMALWDIKSKLAGMPVYQLLGGKSREGLMVYGHANGRDHDEAIDAAHRHIAAGYRAIRVQSGVPGLAKVYG
VGKTAGAYEPAEKGLPPEEPWDTALYLRHTPELFRKVRDALGDAPHLLHDAHHRLTPIEAARLGRDLEPYRLFWLEDATP
AENQDAFRLIRSHTTTTLAVGEVFNSIWDCKDLIRDQLIDYIRATIVHAGGITHVRRIADYAAMYQVRTGFHGATDLSPV
CMAAAVNFGLWAPNFGIQELMPHTALTDEVFPHNYRFDDGYLVMDDVPGLGVDIDETLAANYPYERAYLPVARLRDGSMW
NW

Sequences:

>Translated_402_residues
MKIERLNIIVTCPGRNFVTVKIVTDEGVHGLGDATLNGRELAVRAYLEDHVFPCLIGRDPRDIEDIWQYLYRGAYWRRGP
VTMTAIAAIDMALWDIKSKLAGMPVYQLLGGKSREGLMVYGHANGRDHDEAIDAAHRHIAAGYRAIRVQSGVPGLAKVYG
VGKTAGAYEPAEKGLPPEEPWDTALYLRHTPELFRKVRDALGDAPHLLHDAHHRLTPIEAARLGRDLEPYRLFWLEDATP
AENQDAFRLIRSHTTTTLAVGEVFNSIWDCKDLIRDQLIDYIRATIVHAGGITHVRRIADYAAMYQVRTGFHGATDLSPV
CMAAAVNFGLWAPNFGIQELMPHTALTDEVFPHNYRFDDGYLVMDDVPGLGVDIDETLAANYPYERAYLPVARLRDGSMW
NW
>Mature_402_residues
MKIERLNIIVTCPGRNFVTVKIVTDEGVHGLGDATLNGRELAVRAYLEDHVFPCLIGRDPRDIEDIWQYLYRGAYWRRGP
VTMTAIAAIDMALWDIKSKLAGMPVYQLLGGKSREGLMVYGHANGRDHDEAIDAAHRHIAAGYRAIRVQSGVPGLAKVYG
VGKTAGAYEPAEKGLPPEEPWDTALYLRHTPELFRKVRDALGDAPHLLHDAHHRLTPIEAARLGRDLEPYRLFWLEDATP
AENQDAFRLIRSHTTTTLAVGEVFNSIWDCKDLIRDQLIDYIRATIVHAGGITHVRRIADYAAMYQVRTGFHGATDLSPV
CMAAAVNFGLWAPNFGIQELMPHTALTDEVFPHNYRFDDGYLVMDDVPGLGVDIDETLAANYPYERAYLPVARLRDGSMW
NW

Specific function: Probably involved in the degradation of homoserine lactone (HSL) or of a metabolite of HSL that signals starvation [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=404, Percent_Identity=65.5940594059406, Blast_Score=562, Evalue=1e-161,
Organism=Escherichia coli, GI48994953, Length=413, Percent_Identity=29.5399515738499, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI226510960, Length=348, Percent_Identity=24.1379310344828, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 45097; Mature: 45097

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIERLNIIVTCPGRNFVTVKIVTDEGVHGLGDATLNGRELAVRAYLEDHVFPCLIGRDP
CCEEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCHHEEEEEHHHHCCEEEEECCCC
RDIEDIWQYLYRGAYWRRGPVTMTAIAAIDMALWDIKSKLAGMPVYQLLGGKSREGLMVY
CCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEE
GHANGRDHDEAIDAAHRHIAAGYRAIRVQSGVPGLAKVYGVGKTAGAYEPAEKGLPPEEP
ECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHCCCCCCCCCCCHHCCCCCCCC
WDTALYLRHTPELFRKVRDALGDAPHLLHDAHHRLTPIEAARLGRDLEPYRLFWLEDATP
CCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEECCCC
AENQDAFRLIRSHTTTTLAVGEVFNSIWDCKDLIRDQLIDYIRATIVHAGGITHVRRIAD
CCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
YAAMYQVRTGFHGATDLSPVCMAAAVNFGLWAPNFGIQELMPHTALTDEVFPHNYRFDDG
HHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCHHCCCCCCCCCEECCCC
YLVMDDVPGLGVDIDETLAANYPYERAYLPVARLRDGSMWNW
EEEEECCCCCCCCHHHHHHCCCCCHHHCCCHHHCCCCCCCCC
>Mature Secondary Structure
MKIERLNIIVTCPGRNFVTVKIVTDEGVHGLGDATLNGRELAVRAYLEDHVFPCLIGRDP
CCEEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCHHEEEEEHHHHCCEEEEECCCC
RDIEDIWQYLYRGAYWRRGPVTMTAIAAIDMALWDIKSKLAGMPVYQLLGGKSREGLMVY
CCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEE
GHANGRDHDEAIDAAHRHIAAGYRAIRVQSGVPGLAKVYGVGKTAGAYEPAEKGLPPEEP
ECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHCCCCCCCCCCCHHCCCCCCCC
WDTALYLRHTPELFRKVRDALGDAPHLLHDAHHRLTPIEAARLGRDLEPYRLFWLEDATP
CCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEECCCC
AENQDAFRLIRSHTTTTLAVGEVFNSIWDCKDLIRDQLIDYIRATIVHAGGITHVRRIAD
CCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
YAAMYQVRTGFHGATDLSPVCMAAAVNFGLWAPNFGIQELMPHTALTDEVFPHNYRFDDG
HHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCHHCCCCCCCCCEECCCC
YLVMDDVPGLGVDIDETLAANYPYERAYLPVARLRDGSMWNW
EEEEECCCCCCCCHHHHHHCCCCCHHHCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]