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The map label for this gene is epsE [H]

Identifier: 126447382

GI number: 126447382

Start: 611538

End: 613148

Strand: Direct

Name: epsE [H]

Synonym: BMA10247_A0661

Alternate gene names: 126447382

Gene position: 611538-613148 (Clockwise)

Preceding gene: 126446680

Following gene: 126446654

Centisome position: 25.99

GC content: 71.2

Gene sequence:

>1611_bases
ATGAGCCACGACGCGCAACGACCCGACACGGCCGCCGCGCGCGCGACGCCGAGCGCCGCCGACGCCGCCGCGCCGCCGCG
CGGCCCGCGCGCGGTCAGCGAGGCCGGCGATTTCGCCGCGAGCGTCGAGGAGCGCAAGTTCGTCTGCCTGTTCGACGACG
GGCGGCTGCTGATCGCCGAAGGCCACGAGATGAATCCGTTCGTGCTGTCGTATCGCGCGCGCCTCGACCGGATGGGCCGG
CCGTACCGGCCGACGCCGGCGACGCTGATGCAGGTGCGCGAGGCGTACCGCCAGCACGTGGCGGGCGGCGGCGAGCGGCT
CGACCACACGGTGATGCAGGTGCTCGCGAAGGAGCTGATCGGCCGCGCGTGCCGCGAGCGCGCATCCGACGTGCACATTC
GGGTGCGCCGCTTCAGCACGGAGGTCTACTTCCGGATCCACAACGAACTCATGCGCGTGAACGAGCACACGCGCGAGCAC
GGCGAGCGGCTGCTCGCGACGCTGTATGGCGCGATGACGACCGTCTCGGACAACAGCTACCGGCCGAGCGAGCGGCAGGA
CGCGAGCATCGGCGACCGCGACAAGCTGCCGGACGACCTGTACGGCGTGCGGATCGCGACGACGCCGACGAGCGAGGGCA
GCCTGATGGTGCTGCGGCTGCTGTACAACGATGCGGGCGACGCGACCGATCTCGCGGCGCTCGGCTTCGCGCCCGAGCAC
GTCGCCGCGTTCCGCATGCTGCGCGCGCAGCCGCACGGGATGAACATCATCAGCGGCCCGACGGGCTCCGGCAAGTCGAC
GACGCTGCAGCGCATGCTCGCCGCGCAGATCGACGAATCGCACGGCAGCCTGCACGTGATCACCGTCGAGGATCCGATCG
AATATCCGATCGACGGCGCGGTGCAGACGCCGGTGGCGAACGCGCCGACGGAGGACGCCCGCGCGCTCGCGTTCGCGGCG
GCGATCACCAACGCGATGCGGCTCGATCCGGACACGATCATGATCGGCGAGATCCGCGATCGCGTGTCCGGGCAGACGGC
GCTGCGCGCGTCGATGACGGGCCATCAGGTGTGGACGACCGTGCACGCGAACAGTGCGCTCGCGATCGCCGATCGCCTGA
TCGATCTCGGCCTGCACGCGCGGATGATCACCGATCACACGGTGATCTCCGGGCTCATCAGCCAGCGCCTCGTGAAGCTG
CTGTGCCCGCACTGCAAGGTGCGGCTCCTCGATCATGCGGAGCGGATCGAGCCGGGCCTGCTCGCGCGGCTGCGGCTCGC
GCTCGACACCCGGATGAGCGAGGTCTGCATCACGGGCGACGGCTGCGAGCAGTGCGGCATGGCCGGCACGATCGGCCGCA
CGGTGGTGGCCGAGGTGATCCTGCCCGACGCGCGGCTCTTCGAGTTCCTGCGCGACGGCGACAAGGTCGGCGCGCTCGAG
TACTGCACCCGCACGCTCGGCGCGATGACGCTCGCCGAGCACGCACTGCGCAAGGTCGCGGCGGGGCTCGTCGATCCGCG
CGGCGTCGAGCGCGTGGTCGGCGCGCTCGCGCCGGTGACGGGCGACGCGCGACAGCAGCTGTCGCTCGTCGGATTCAGCT
ATGGCACTTGA

Upstream 100 bases:

>100_bases
CGGGCGCGCCGCCGTCGCCTGCCTATGCGGGCAATCCGCCGCTGCCGGCCGACGCGGCCGCGCCGCTGCCGACGTTGCCC
GCGCGGCCGGCGAGCGGATC

Downstream 100 bases:

>100_bases
GCTGAACCGCCGCTGGGCGAAGCTCTGCCTGAACGCCGACGAGCGGCTGCGCGTCTACCGGAAGATCGCGAAGATGCTCG
GCAACGGGCTGCCGTTGCTG

Product: type II/IV secretion system protein

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MSHDAQRPDTAAARATPSAADAAAPPRGPRAVSEAGDFAASVEERKFVCLFDDGRLLIAEGHEMNPFVLSYRARLDRMGR
PYRPTPATLMQVREAYRQHVAGGGERLDHTVMQVLAKELIGRACRERASDVHIRVRRFSTEVYFRIHNELMRVNEHTREH
GERLLATLYGAMTTVSDNSYRPSERQDASIGDRDKLPDDLYGVRIATTPTSEGSLMVLRLLYNDAGDATDLAALGFAPEH
VAAFRMLRAQPHGMNIISGPTGSGKSTTLQRMLAAQIDESHGSLHVITVEDPIEYPIDGAVQTPVANAPTEDARALAFAA
AITNAMRLDPDTIMIGEIRDRVSGQTALRASMTGHQVWTTVHANSALAIADRLIDLGLHARMITDHTVISGLISQRLVKL
LCPHCKVRLLDHAERIEPGLLARLRLALDTRMSEVCITGDGCEQCGMAGTIGRTVVAEVILPDARLFEFLRDGDKVGALE
YCTRTLGAMTLAEHALRKVAAGLVDPRGVERVVGALAPVTGDARQQLSLVGFSYGT

Sequences:

>Translated_536_residues
MSHDAQRPDTAAARATPSAADAAAPPRGPRAVSEAGDFAASVEERKFVCLFDDGRLLIAEGHEMNPFVLSYRARLDRMGR
PYRPTPATLMQVREAYRQHVAGGGERLDHTVMQVLAKELIGRACRERASDVHIRVRRFSTEVYFRIHNELMRVNEHTREH
GERLLATLYGAMTTVSDNSYRPSERQDASIGDRDKLPDDLYGVRIATTPTSEGSLMVLRLLYNDAGDATDLAALGFAPEH
VAAFRMLRAQPHGMNIISGPTGSGKSTTLQRMLAAQIDESHGSLHVITVEDPIEYPIDGAVQTPVANAPTEDARALAFAA
AITNAMRLDPDTIMIGEIRDRVSGQTALRASMTGHQVWTTVHANSALAIADRLIDLGLHARMITDHTVISGLISQRLVKL
LCPHCKVRLLDHAERIEPGLLARLRLALDTRMSEVCITGDGCEQCGMAGTIGRTVVAEVILPDARLFEFLRDGDKVGALE
YCTRTLGAMTLAEHALRKVAAGLVDPRGVERVVGALAPVTGDARQQLSLVGFSYGT
>Mature_535_residues
SHDAQRPDTAAARATPSAADAAAPPRGPRAVSEAGDFAASVEERKFVCLFDDGRLLIAEGHEMNPFVLSYRARLDRMGRP
YRPTPATLMQVREAYRQHVAGGGERLDHTVMQVLAKELIGRACRERASDVHIRVRRFSTEVYFRIHNELMRVNEHTREHG
ERLLATLYGAMTTVSDNSYRPSERQDASIGDRDKLPDDLYGVRIATTPTSEGSLMVLRLLYNDAGDATDLAALGFAPEHV
AAFRMLRAQPHGMNIISGPTGSGKSTTLQRMLAAQIDESHGSLHVITVEDPIEYPIDGAVQTPVANAPTEDARALAFAAA
ITNAMRLDPDTIMIGEIRDRVSGQTALRASMTGHQVWTTVHANSALAIADRLIDLGLHARMITDHTVISGLISQRLVKLL
CPHCKVRLLDHAERIEPGLLARLRLALDTRMSEVCITGDGCEQCGMAGTIGRTVVAEVILPDARLFEFLRDGDKVGALEY
CTRTLGAMTLAEHALRKVAAGLVDPRGVERVVGALAPVTGDARQQLSLVGFSYGT

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=403, Percent_Identity=31.0173697270471, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1786296, Length=431, Percent_Identity=29.9303944315545, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI87082188, Length=163, Percent_Identity=34.9693251533742, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 58325; Mature: 58194

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHDAQRPDTAAARATPSAADAAAPPRGPRAVSEAGDFAASVEERKFVCLFDDGRLLIAE
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCEEEEEC
GHEMNPFVLSYRARLDRMGRPYRPTPATLMQVREAYRQHVAGGGERLDHTVMQVLAKELI
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
GRACRERASDVHIRVRRFSTEVYFRIHNELMRVNEHTREHGERLLATLYGAMTTVSDNSY
HHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RPSERQDASIGDRDKLPDDLYGVRIATTPTSEGSLMVLRLLYNDAGDATDLAALGFAPEH
CCCCCCCCCCCCCCCCCCHHHEEEEEECCCCCCCEEEEEEHHCCCCCHHHHHHHCCCHHH
VAAFRMLRAQPHGMNIISGPTGSGKSTTLQRMLAAQIDESHGSLHVITVEDPIEYPIDGA
HHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
VQTPVANAPTEDARALAFAAAITNAMRLDPDTIMIGEIRDRVSGQTALRASMTGHQVWTT
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHCCCHHHHHHCCCCEEEEE
VHANSALAIADRLIDLGLHARMITDHTVISGLISQRLVKLLCPHCKVRLLDHAERIEPGL
EECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHH
LARLRLALDTRMSEVCITGDGCEQCGMAGTIGRTVVAEVILPDARLFEFLRDGDKVGALE
HHHHHHHHHHCCHHEEECCCCHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHH
YCTRTLGAMTLAEHALRKVAAGLVDPRGVERVVGALAPVTGDARQQLSLVGFSYGT
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
SHDAQRPDTAAARATPSAADAAAPPRGPRAVSEAGDFAASVEERKFVCLFDDGRLLIAE
CCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCEEEEEC
GHEMNPFVLSYRARLDRMGRPYRPTPATLMQVREAYRQHVAGGGERLDHTVMQVLAKELI
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
GRACRERASDVHIRVRRFSTEVYFRIHNELMRVNEHTREHGERLLATLYGAMTTVSDNSY
HHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RPSERQDASIGDRDKLPDDLYGVRIATTPTSEGSLMVLRLLYNDAGDATDLAALGFAPEH
CCCCCCCCCCCCCCCCCCHHHEEEEEECCCCCCCEEEEEEHHCCCCCHHHHHHHCCCHHH
VAAFRMLRAQPHGMNIISGPTGSGKSTTLQRMLAAQIDESHGSLHVITVEDPIEYPIDGA
HHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
VQTPVANAPTEDARALAFAAAITNAMRLDPDTIMIGEIRDRVSGQTALRASMTGHQVWTT
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHCCCHHHHHHCCCCEEEEE
VHANSALAIADRLIDLGLHARMITDHTVISGLISQRLVKLLCPHCKVRLLDHAERIEPGL
EECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHH
LARLRLALDTRMSEVCITGDGCEQCGMAGTIGRTVVAEVILPDARLFEFLRDGDKVGALE
HHHHHHHHHHCCHHEEECCCCHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHH
YCTRTLGAMTLAEHALRKVAAGLVDPRGVERVVGALAPVTGDARQQLSLVGFSYGT
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]