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The map label for this gene is cysT [H]

Identifier: 126209308

GI number: 126209308

Start: 2077084

End: 2077896

Strand: Direct

Name: cysT [H]

Synonym: APL_1846

Alternate gene names: 126209308

Gene position: 2077084-2077896 (Clockwise)

Preceding gene: 126209307

Following gene: 126209309

Centisome position: 91.32

GC content: 42.19

Gene sequence:

>813_bases
ATGCAACAAACTCGAATTATTCCCGGTTTTAACCTTTCGTTAGGTTATACCCTCACATTTTTAAGCCTTGTAGTACTGAT
TCCGTTAGCCGGTCTATTTATCTTTTCCGCCCAAATTTCTGCCGAACAATTTATTAAATTGATTACCAGCCGGCAGTTAT
TATTAGCGTTGGGGTTATCTTTTAAAAGCGCATTCAGTGCCGCATTAGTGAATAGTTTTATCGGTTTATTACTTGCTTGG
GTGTTAGCTCGCTATCAGTTTAGGGGAAAACGTTTGATGGATACCTTTATTGATATGCCGTTTGCCTTGCCAACTGCGGT
AGCTGGCATTGCACTGACGGCGATTTACGCTCGCAACGGTATTATCGGGCAATTTTTTGAAACCAAAATCGCCTACAGCT
CATTAGGTATTACTTTAGCATTATTGTTTGTCACGTTGCCCTTTGTCGTTCGCACCTTACAACCGGTGCTGGAAGATTTA
CCGAAAGATGTAGAAGAAGCGGCAGAAATTTTAGGTGCCAGTCCGTTTCAGACCTTCCGCTATGTGATTTTTCCGGCAAT
TTTACCGGCTTGGATTACTGGTTTTACCCTCGCTTTTGCACGAGGCATCGGCGAATACGGTTCGGTCGTATTTATTTCCG
GTAATTTGCCGTTTAGAACCGAAATTCTACCTCTAGTGATTATGTCTAAGCTCGATCAATACGATTATGTCGGGGCGGCA
ACTATTGGTGCATTAATGTTGATCATCTCATTCGTACTGATTTTTGTGATCAACCTTATTCAGCGTTACTTCGCTAAGCG
GACAGCAAAATAG

Upstream 100 bases:

>100_bases
CGGCGGATGGGAATTAATTCAGCCAAAATTTTTTAATGACGGTGCAATCTTCGATCAAATCTTAGAAAAAATTTACCAAT
AAATAGGTTAAGGAAATGTT

Downstream 100 bases:

>100_bases
AAGGGAAGAAATATGCAAAGTTTACTCAAAAAACGACCGCTTGTTGAAAGACTATTAATCGGCTTAGCGATTCTGCTATT
TGTGATTTTGCTACTTTTAC

Product: Sulfate transport system permease protein cysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MQQTRIIPGFNLSLGYTLTFLSLVVLIPLAGLFIFSAQISAEQFIKLITSRQLLLALGLSFKSAFSAALVNSFIGLLLAW
VLARYQFRGKRLMDTFIDMPFALPTAVAGIALTAIYARNGIIGQFFETKIAYSSLGITLALLFVTLPFVVRTLQPVLEDL
PKDVEEAAEILGASPFQTFRYVIFPAILPAWITGFTLAFARGIGEYGSVVFISGNLPFRTEILPLVIMSKLDQYDYVGAA
TIGALMLIISFVLIFVINLIQRYFAKRTAK

Sequences:

>Translated_270_residues
MQQTRIIPGFNLSLGYTLTFLSLVVLIPLAGLFIFSAQISAEQFIKLITSRQLLLALGLSFKSAFSAALVNSFIGLLLAW
VLARYQFRGKRLMDTFIDMPFALPTAVAGIALTAIYARNGIIGQFFETKIAYSSLGITLALLFVTLPFVVRTLQPVLEDL
PKDVEEAAEILGASPFQTFRYVIFPAILPAWITGFTLAFARGIGEYGSVVFISGNLPFRTEILPLVIMSKLDQYDYVGAA
TIGALMLIISFVLIFVINLIQRYFAKRTAK
>Mature_270_residues
MQQTRIIPGFNLSLGYTLTFLSLVVLIPLAGLFIFSAQISAEQFIKLITSRQLLLALGLSFKSAFSAALVNSFIGLLLAW
VLARYQFRGKRLMDTFIDMPFALPTAVAGIALTAIYARNGIIGQFFETKIAYSSLGITLALLFVTLPFVVRTLQPVLEDL
PKDVEEAAEILGASPFQTFRYVIFPAILPAWITGFTLAFARGIGEYGSVVFISGNLPFRTEILPLVIMSKLDQYDYVGAA
TIGALMLIISFVLIFVINLIQRYFAKRTAK

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=266, Percent_Identity=44.7368421052632, Blast_Score=220, Evalue=7e-59,
Organism=Escherichia coli, GI87082099, Length=252, Percent_Identity=33.3333333333333, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1786980, Length=212, Percent_Identity=32.5471698113208, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1787368, Length=216, Percent_Identity=30.0925925925926, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787713, Length=166, Percent_Identity=28.3132530120482, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29770; Mature: 29770

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]