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The map label for this gene is sbp [H]

Identifier: 126209307

GI number: 126209307

Start: 2076271

End: 2077065

Strand: Direct

Name: sbp [H]

Synonym: APL_1845

Alternate gene names: 126209307

Gene position: 2076271-2077065 (Clockwise)

Preceding gene: 126209303

Following gene: 126209308

Centisome position: 91.29

GC content: 45.28

Gene sequence:

>795_bases
GTGATTGACGGCTTGCAAGCGGATGTCGTTACCTTAGCGTTAGCCGGTGATATTGATGCGTTAAATAAACATCAAGATCT
GATCGATCCGAATTGGCGGCAACGTTTGAGCGAATGCAGTTTGCCGTATCGCTCGACCATTGTGTTTTTAGTGCGTAAAG
GGAATCCGAAACGGATCTATGACTGGCAAGATTTAGTGCGAGATCCGAGCATTGAAGTGATTACACCGAACCCAAAAACC
TCCGGCGGTGCAAGATGGAATTTTCTGGCGGCTTGGGCGTATGCTCAAGATCGTTTTGGTAGGGAGCAGCAAGCATTGGA
ATTTGTGACTCGCCTTTATCAACAAGTGCCGGTATTAGATACCGGCGCGAGAGGCGCGACGATGAGTTTTATCCAGCGAG
GCTTGGGCGATGTCTTACTTGCTTGGGAAAATGAGGCTTATTTGGCATTACAAGAAGAAGGGGGTGATCAATTTGAAATC
ATCACGCCTAAAGTCTCGATTGTTGCCGAACCACCGGTTGCAGTGGTGGATAAAGTGGTGGATAAAAAAGGCACTCGTAA
ACAGGCGGAGGCTTATCTGAACTATTTGTATAGCGATGCAGCACAACGCCTGATTGCCAAACATTATTATCGCCCTGCAA
GCGGTCAAATTTTGGCAGAATTTCACCAACAATTTCCTGAGTTAAGACTACTTAGAATGCACGACATCTTTGGCGGCGGA
TGGGAATTAATTCAGCCAAAATTTTTTAATGACGGTGCAATCTTCGATCAAATCTTAGAAAAAATTTACCAATAA

Upstream 100 bases:

>100_bases
GGCGTTTGGTTTGCGGTAACAGTTCTACTTCCGTCCGTGAAAAATCTAATTGTGAAGCATCAATTAATACTACGGCGGCT
CAGGCAAACAAGCTCGTGCG

Downstream 100 bases:

>100_bases
ATAGGTTAAGGAAATGTTATGCAACAAACTCGAATTATTCCCGGTTTTAACCTTTCGTTAGGTTATACCCTCACATTTTT
AAGCCTTGTAGTACTGATTC

Product: sulfate-binding protein precursor

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MIDGLQADVVTLALAGDIDALNKHQDLIDPNWRQRLSECSLPYRSTIVFLVRKGNPKRIYDWQDLVRDPSIEVITPNPKT
SGGARWNFLAAWAYAQDRFGREQQALEFVTRLYQQVPVLDTGARGATMSFIQRGLGDVLLAWENEAYLALQEEGGDQFEI
ITPKVSIVAEPPVAVVDKVVDKKGTRKQAEAYLNYLYSDAAQRLIAKHYYRPASGQILAEFHQQFPELRLLRMHDIFGGG
WELIQPKFFNDGAIFDQILEKIYQ

Sequences:

>Translated_264_residues
MIDGLQADVVTLALAGDIDALNKHQDLIDPNWRQRLSECSLPYRSTIVFLVRKGNPKRIYDWQDLVRDPSIEVITPNPKT
SGGARWNFLAAWAYAQDRFGREQQALEFVTRLYQQVPVLDTGARGATMSFIQRGLGDVLLAWENEAYLALQEEGGDQFEI
ITPKVSIVAEPPVAVVDKVVDKKGTRKQAEAYLNYLYSDAAQRLIAKHYYRPASGQILAEFHQQFPELRLLRMHDIFGGG
WELIQPKFFNDGAIFDQILEKIYQ
>Mature_264_residues
MIDGLQADVVTLALAGDIDALNKHQDLIDPNWRQRLSECSLPYRSTIVFLVRKGNPKRIYDWQDLVRDPSIEVITPNPKT
SGGARWNFLAAWAYAQDRFGREQQALEFVTRLYQQVPVLDTGARGATMSFIQRGLGDVLLAWENEAYLALQEEGGDQFEI
ITPKVSIVAEPPVAVVDKVVDKKGTRKQAEAYLNYLYSDAAQRLIAKHYYRPASGQILAEFHQQFPELRLLRMHDIFGGG
WELIQPKFFNDGAIFDQILEKIYQ

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=262, Percent_Identity=55.7251908396947, Blast_Score=297, Evalue=4e-82,
Organism=Escherichia coli, GI1788765, Length=260, Percent_Identity=44.2307692307692, Blast_Score=220, Evalue=6e-59,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 30072; Mature: 30072

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDGLQADVVTLALAGDIDALNKHQDLIDPNWRQRLSECSLPYRSTIVFLVRKGNPKRIY
CCCCHHHHHEEEEECCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCEEE
DWQDLVRDPSIEVITPNPKTSGGARWNFLAAWAYAQDRFGREQQALEFVTRLYQQVPVLD
CHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
TGARGATMSFIQRGLGDVLLAWENEAYLALQEEGGDQFEIITPKVSIVAEPPVAVVDKVV
CCCCCHHHHHHHHCCCCEEEEECCCEEEEEECCCCCEEEEECCCEEEEECCCHHHHHHHH
DKKGTRKQAEAYLNYLYSDAAQRLIAKHYYRPASGQILAEFHQQFPELRLLRMHDIFGGG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHCCCC
WELIQPKFFNDGAIFDQILEKIYQ
HHHCCCEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MIDGLQADVVTLALAGDIDALNKHQDLIDPNWRQRLSECSLPYRSTIVFLVRKGNPKRIY
CCCCHHHHHEEEEECCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCEEE
DWQDLVRDPSIEVITPNPKTSGGARWNFLAAWAYAQDRFGREQQALEFVTRLYQQVPVLD
CHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
TGARGATMSFIQRGLGDVLLAWENEAYLALQEEGGDQFEIITPKVSIVAEPPVAVVDKVV
CCCCCHHHHHHHHCCCCEEEEECCCEEEEEECCCCCEEEEECCCEEEEECCCHHHHHHHH
DKKGTRKQAEAYLNYLYSDAAQRLIAKHYYRPASGQILAEFHQQFPELRLLRMHDIFGGG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHCCCC
WELIQPKFFNDGAIFDQILEKIYQ
HHHCCCEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]