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The map label for this gene is def [H]

Identifier: 126209175

GI number: 126209175

Start: 1942275

End: 1942739

Strand: Reverse

Name: def [H]

Synonym: APL_1711

Alternate gene names: 126209175

Gene position: 1942739-1942275 (Counterclockwise)

Preceding gene: 126209177

Following gene: 126209174

Centisome position: 85.41

GC content: 43.44

Gene sequence:

>465_bases
GTGTGTGAGCCTGTCGCACAGGTGGACGATGAATTAAATCAGTTCATCGATGATATGTTTGACACTATGTACGAACACGA
AGGTATCGGCCTTGCCGCACCGCAAGTGGGCGTATTAAAACGTGTTATTACCATTGATATTGAAGGCGATAAAACTAATC
AAGTGGTACTGATTAACCCTGAGATTTTAGAATCATGCGGCGAAACCGGTATCGAAGAAGGTTGTTTATCGATCCCCGGT
CATCGTGCGTTAGTGCCGCGTAAAGAAAAAGTGAAAGTCAAAGCGTTAAATCGCAAAGGCGAAGAAGTGATTTACGAAGC
GGACGGTTTATTCGCCATCTGTATCCAACACGAAATCGACCACCTCAACGGTGTGCTGTTCGTGGATCACATCTCTGCAC
TTAAACGCCAACGCATTAAAGAAAAAATGCAAAAACTCAAAAAACAAATTGAGCGTGCGAAATAA

Upstream 100 bases:

>100_bases
AGTTAATTAACCAATTTATAATTTTTTATTTTTCGATTGAGATCACAAAAAAATGTCAGTTTTAGAAGTTGTACTTTACC
CGGATGAAGGGTTAGCAAAA

Downstream 100 bases:

>100_bases
TCCTACCCATTAAGAAAAGCGAGCATTGTCTCGCTTTTTCTTTGTTTACTAGCAAGCGGTCTAATTTGCTGCAAAATTTG
CAAATCCGCCCCAATCCGCC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MCEPVAQVDDELNQFIDDMFDTMYEHEGIGLAAPQVGVLKRVITIDIEGDKTNQVVLINPEILESCGETGIEEGCLSIPG
HRALVPRKEKVKVKALNRKGEEVIYEADGLFAICIQHEIDHLNGVLFVDHISALKRQRIKEKMQKLKKQIERAK

Sequences:

>Translated_154_residues
MCEPVAQVDDELNQFIDDMFDTMYEHEGIGLAAPQVGVLKRVITIDIEGDKTNQVVLINPEILESCGETGIEEGCLSIPG
HRALVPRKEKVKVKALNRKGEEVIYEADGLFAICIQHEIDHLNGVLFVDHISALKRQRIKEKMQKLKKQIERAK
>Mature_154_residues
MCEPVAQVDDELNQFIDDMFDTMYEHEGIGLAAPQVGVLKRVITIDIEGDKTNQVVLINPEILESCGETGIEEGCLSIPG
HRALVPRKEKVKVKALNRKGEEVIYEADGLFAICIQHEIDHLNGVLFVDHISALKRQRIKEKMQKLKKQIERAK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=142, Percent_Identity=31.6901408450704, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1789682, Length=153, Percent_Identity=58.1699346405229, Blast_Score=187, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24645728, Length=146, Percent_Identity=32.8767123287671, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24645726, Length=141, Percent_Identity=28.3687943262411, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 17374; Mature: 17374

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCEPVAQVDDELNQFIDDMFDTMYEHEGIGLAAPQVGVLKRVITIDIEGDKTNQVVLINP
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHEEEEEECCCCCCEEEEECH
EILESCGETGIEEGCLSIPGHRALVPRKEKVKVKALNRKGEEVIYEADGLFAICIQHEID
HHHHHHCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCEEEEHHHHHHH
HLNGVLFVDHISALKRQRIKEKMQKLKKQIERAK
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCEPVAQVDDELNQFIDDMFDTMYEHEGIGLAAPQVGVLKRVITIDIEGDKTNQVVLINP
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHEEEEEECCCCCCEEEEECH
EILESCGETGIEEGCLSIPGHRALVPRKEKVKVKALNRKGEEVIYEADGLFAICIQHEID
HHHHHHCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCEEEEHHHHHHH
HLNGVLFVDHISALKRQRIKEKMQKLKKQIERAK
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA