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The map label for this gene is rfaD

Identifier: 126209174

GI number: 126209174

Start: 1941209

End: 1942135

Strand: Reverse

Name: rfaD

Synonym: APL_1710

Alternate gene names: 126209174

Gene position: 1942135-1941209 (Counterclockwise)

Preceding gene: 126209175

Following gene: 126209169

Centisome position: 85.39

GC content: 41.64

Gene sequence:

>927_bases
ATGATTATCGTAACAGGCGGCTTTGGTATGATCGGTAGCAATATCGTCAAAGCATTAAATGAAATCGGCAGAAAAGATAT
TCTTGTTGTAGATAACTTAAAAAACGGCGAAAAATTCGTAAATTTAGTAGATTTAGATATTGCGGATTATTGTGATAAAG
AAGATTTTATCGCTTCGATCATTGCCGGCGATGATTTCGGCGAGATTGATGCGGTATTCCACGAAGGTGCTTGCTCAGCA
ACTACCGAATGGGACGGTAAATACTTAATGCACAATAACTACGAATATTCAAAAGAGTTATTACATTTCTGTTTAGATCA
CCAAATTCCGTTCTTCTACGCATCAAGTGCGGCAACTTACGGCGGTCGTTCGGATAACTTTATTGAAGAGCGTAAATTTG
AACAGCCGCTCAATGCTTACGGCTATTCAAAATTCCTATTTGACGAATATGTACGTCAAGTTTTACCGGAAGCGGATTCG
CCGGTATGCGGTTTTAAATATTTCAACGTGTACGGCCCGCGTGAACAACACAAAGGCTCAATGGCAAGCGTAGCGTTCCA
CTTAAACAATCAAATGTTAAAAGGTGAAAACCCGAAATTATTCGAGGGATCAGAAACCTTCTTACGTGATTTCGTGTATG
TGGAAGACGTAGCGAAAGTGAATATTTGGGCGTGGCAAAACGGTATTTCCGGCATTTATAACCTCGGTACCGGCAAAGCG
GAATCATTCCAAGCGGTGGCACAAGCGGTGATCGACTTCCACGGCAAAGGCGAAATCGAAAAAATCCCATTCCCGGATCA
TTTAAAATCTCGCTACCAAACCTTCACCCAAGCGGATTTAACTAAACTCCGTGCAGCAGGCTATACAGGTACATTTAAAA
CCGTAGCCGAAGGTACGAAAGAATATATGGCGTGGTTAAATCGTTAA

Upstream 100 bases:

>100_bases
CTTTGTTTACTAGCAAGCGGTCTAATTTGCTGCAAAATTTGCAAATCCGCCCCAATCCGCCGATAATATCCCCGTTTTTA
ATTTCAAAAAGGAAAAAGCT

Downstream 100 bases:

>100_bases
TTCATTCCTAACAAGCGGTTGTTTTTGCGGTTTTCTTACCAAAAACAACCGCTTGTAAACTACCAATTCTTTTCTACCGT
CATAAACCATTCACTGCTCG

Product: ADP-L-glycero-D-mannoheptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MIIVTGGFGMIGSNIVKALNEIGRKDILVVDNLKNGEKFVNLVDLDIADYCDKEDFIASIIAGDDFGEIDAVFHEGACSA
TTEWDGKYLMHNNYEYSKELLHFCLDHQIPFFYASSAATYGGRSDNFIEERKFEQPLNAYGYSKFLFDEYVRQVLPEADS
PVCGFKYFNVYGPREQHKGSMASVAFHLNNQMLKGENPKLFEGSETFLRDFVYVEDVAKVNIWAWQNGISGIYNLGTGKA
ESFQAVAQAVIDFHGKGEIEKIPFPDHLKSRYQTFTQADLTKLRAAGYTGTFKTVAEGTKEYMAWLNR

Sequences:

>Translated_308_residues
MIIVTGGFGMIGSNIVKALNEIGRKDILVVDNLKNGEKFVNLVDLDIADYCDKEDFIASIIAGDDFGEIDAVFHEGACSA
TTEWDGKYLMHNNYEYSKELLHFCLDHQIPFFYASSAATYGGRSDNFIEERKFEQPLNAYGYSKFLFDEYVRQVLPEADS
PVCGFKYFNVYGPREQHKGSMASVAFHLNNQMLKGENPKLFEGSETFLRDFVYVEDVAKVNIWAWQNGISGIYNLGTGKA
ESFQAVAQAVIDFHGKGEIEKIPFPDHLKSRYQTFTQADLTKLRAAGYTGTFKTVAEGTKEYMAWLNR
>Mature_308_residues
MIIVTGGFGMIGSNIVKALNEIGRKDILVVDNLKNGEKFVNLVDLDIADYCDKEDFIASIIAGDDFGEIDAVFHEGACSA
TTEWDGKYLMHNNYEYSKELLHFCLDHQIPFFYASSAATYGGRSDNFIEERKFEQPLNAYGYSKFLFDEYVRQVLPEADS
PVCGFKYFNVYGPREQHKGSMASVAFHLNNQMLKGENPKLFEGSETFLRDFVYVEDVAKVNIWAWQNGISGIYNLGTGKA
ESFQAVAQAVIDFHGKGEIEKIPFPDHLKSRYQTFTQADLTKLRAAGYTGTFKTVAEGTKEYMAWLNR

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily

Homologues:

Organism=Escherichia coli, GI1790049, Length=308, Percent_Identity=75, Blast_Score=495, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI71982035, Length=315, Percent_Identity=27.9365079365079, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71982038, Length=317, Percent_Identity=27.7602523659306, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): HLDD_ACTP2 (A3N308)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054399.1
- ProteinModelPortal:   A3N308
- SMR:   A3N308
- STRING:   A3N308
- GeneID:   4849464
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1710
- eggNOG:   COG0451
- HOGENOM:   HBG755066
- OMA:   GFIGSAM
- PhylomeDB:   A3N308
- ProtClustDB:   PRK11150
- BioCyc:   APLE416269:APL_1710-MONOMER
- HAMAP:   MF_01601
- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10366:SF29
- TIGRFAMs:   TIGR02197

Pfam domain/function: PF01370 Epimerase

EC number: =5.1.3.20

Molecular weight: Translated: 34770; Mature: 34770

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: ACT_SITE 140-140 ACT_SITE 178-178 BINDING 38-38 BINDING 53-53 BINDING 92-92 BINDING 144-144 BINDING 169-169 BINDING 170-170 BINDING 178-178 BINDING 180-180 BINDING 187-187 BINDING 209-209 BINDING 272-272

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIVTGGFGMIGSNIVKALNEIGRKDILVVDNLKNGEKFVNLVDLDIADYCDKEDFIASI
CEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHEEEEECCHHHHCCHHHHHHHH
IAGDDFGEIDAVFHEGACSATTEWDGKYLMHNNYEYSKELLHFCLDHQIPFFYASSAATY
HCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCC
GGRSDNFIEERKFEQPLNAYGYSKFLFDEYVRQVLPEADSPVCGFKYFNVYGPREQHKGS
CCCCCCHHHHHHHHCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHCCC
MASVAFHLNNQMLKGENPKLFEGSETFLRDFVYVEDVAKVNIWAWQNGISGIYNLGTGKA
CEEEEEEECCCEECCCCCCEECCCHHHHHHHHHHHHHHHEEEEEECCCCCHHEECCCCCH
ESFQAVAQAVIDFHGKGEIEKIPFPDHLKSRYQTFTQADLTKLRAAGYTGTFKTVAEGTK
HHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
EYMAWLNR
HHHHHHCC
>Mature Secondary Structure
MIIVTGGFGMIGSNIVKALNEIGRKDILVVDNLKNGEKFVNLVDLDIADYCDKEDFIASI
CEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHEEEEECCHHHHCCHHHHHHHH
IAGDDFGEIDAVFHEGACSATTEWDGKYLMHNNYEYSKELLHFCLDHQIPFFYASSAATY
HCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCC
GGRSDNFIEERKFEQPLNAYGYSKFLFDEYVRQVLPEADSPVCGFKYFNVYGPREQHKGS
CCCCCCHHHHHHHHCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHCCC
MASVAFHLNNQMLKGENPKLFEGSETFLRDFVYVEDVAKVNIWAWQNGISGIYNLGTGKA
CEEEEEEECCCEECCCCCCEECCCHHHHHHHHHHHHHHHEEEEEECCCCCHHEECCCCCH
ESFQAVAQAVIDFHGKGEIEKIPFPDHLKSRYQTFTQADLTKLRAAGYTGTFKTVAEGTK
HHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
EYMAWLNR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA