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The map label for this gene is mtlA [H]

Identifier: 126209094

GI number: 126209094

Start: 1853283

End: 1855172

Strand: Reverse

Name: mtlA [H]

Synonym: APL_1630

Alternate gene names: 126209094

Gene position: 1855172-1853283 (Counterclockwise)

Preceding gene: 126209095

Following gene: 126209093

Centisome position: 81.56

GC content: 43.28

Gene sequence:

>1890_bases
ATGTCTAATTTAAAAGTGAAAGTCCAAAGTTTTGGGCGTTTCTTATCAAATATGGTGATGCCGAATATCGGAGCATTCAT
CGCTTGGGGTTTTATTACCGCATTATTTATTCCGACCGGTTGGTTACCGAACGAAAGCTTTGCAAAACTTGTCGGCCCGA
TGATCAGCTATCTATTACCGCTTTTAATCGGTTATAGCGGCGGTCGTTTAGCCGGCGGCGAACGTGGTGCTGTGGTCGGT
GCGATTACCACTATGGGTATCATTGTCGGTTCTGAAATTCCGATGTTCTTGGGTGCGATGATTGTCGGTCCTCTTGGCGG
TTGGGTAATCAAAACATTCGATAAAGCAATCGAAGGTAAAGTGAAAAGCGGTTTCGAGATGTTAGTGAATAACTTCTCAG
CCGGTATTATCGGTATGTTGTTAGCGTTACTGTCATTCAGCGTGATCGGTGGTGTGGTAACTTCAATTTCGGATTTATTA
GCGGCGGGCGTTAAAGCGTTGGTTGATGCAAATCTTTTAGCTTTAACTTCAATCATTGTCGAACCGGCAAAAATCTTATT
CTTAAATAACGCTATCAACCACGGTATCTTCTCACCGCTTGGTATTCAAGAATCTTCAGAATTAGGTAAATCGATCTTCT
TCTTAATTGAAGCGAATCCGGGTCCGGGTTTAGGGATTTTGATTGCTTATATGTTATTCGGTAAAGGTTCTGCGAAAAAT
ACCGCAAGTTCCGCATCAATCATTCACTTCTTCGGCGGTATTCATGAAATTTACTTCCCGTATGTATTAATGAATCCTCG
TTTAATCCTTGCGGTAATTGCAGGCGGTGCGACAGGCGTATTAACCAATACATTATTAGGCGGCGGTTTAGTTTCTCCGG
CTTCTCCGGGTTCAATCATTGCTGTATTGGCAATGACACCGGCAGGTGCGCATTTAGCGGTAGTCGCTTCGGTGATTCTT
TCTTGTGCGGTAACTTTTGTCATTGCTTCTGTGTTACTTAAAATGCAAAAAGATGACGGTTCTGAAGAAGATGCGTTAGA
AAGTGCGCAAGCGGAAATGGCGGCGATGAAAGCACAAAGCAAAGGCGGTGTAGCTTCGGTAGCAGGCGATTTTAGTAATA
TACGTAAAATCTATGTATCTTGTGATGCCGGTATGGGCTCAAGTGCAATGGGTGCAAGTATGTTACGTAAAAAAGTACAA
GCGGCAGGTTTGAATATTGAAGTGCAAAATATTGCGATCAACGATTTACCGCAAAACGCACAATTAGTGGTAACCCACAA
AGACTTAACCGCTCGTGCGCAAAAACAAGTACCGAGCGCACAACATTTGTCCTTAAATAACTTCTTAGACGGCGCATTCT
ATGACAGTCTAGTAGCAAAACTTAATACCAATACCGCTCCGGTGGAAAAAGCGACACAAGTAGAAACTGCTCCGCAAGCG
GAAACGGCAAGTACCGGTTTAAACGTTACCGAAGCACAAATCTTCTTAAATTTAACCGCTCGTACCAAAGAAGAAGCGAT
TCAATTTGCCGGCGAGCAATTAGTTAAATTAGGCTTTGCGGAAGAGGATTATATCCCTGCGATGTTTGAGCGTGAGCAAC
AAGTTTCCACTTACTTAGGCGAAGGGATTGCGGTTCCGCACGGTACGATTGCGGCAAAAGATAAAGTCTTAAAAACCGGT
ATCGTATTCTGCCAATATCGTGAAGGCGTACGTTTTACTGATGAAGAAGACGGCGTAGCGAAATTAGTGATCGGTATCGC
GGCTCGTAATAACGAACATATCCAAGTGGTGAGTGCGATTACCAATGCGTTAGACGATGAGAAAGCCATGGAAATTCTCA
CTTTAACCGATGATCCGCAAAAAGTATTACGCTTATTAAAAGGCGAATAA

Upstream 100 bases:

>100_bases
AAATAAATATTTTTGAGACTTTGCTTTCCGGTGCTAATTTATGCGCCAGATTATTCAATCTTATTTATTTAATTCACACA
CTGTTGTTCTAGGAGCAGTT

Downstream 100 bases:

>100_bases
TTCAAGTTCCCCCACGTAAGTGGGGGATTTTCGCTTACAAGCGGTCGGATTTTTCAAATATTTTACAAATTAAAGAGGAA
CATAGAATGAACGCATTACA

Product: PTS system mannitol-specific EIICBA component

Products: NA

Alternate protein names: EIICBA-Mtl; EII-Mtl; Mannitol permease IIC component; PTS system mannitol-specific EIIC component; Mannitol-specific phosphotransferase enzyme IIB component; PTS system mannitol-specific EIIB component; Mannitol-specific phosphotransferase enzyme IIA component; PTS system mannitol-specific EIIA component [H]

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MSNLKVKVQSFGRFLSNMVMPNIGAFIAWGFITALFIPTGWLPNESFAKLVGPMISYLLPLLIGYSGGRLAGGERGAVVG
AITTMGIIVGSEIPMFLGAMIVGPLGGWVIKTFDKAIEGKVKSGFEMLVNNFSAGIIGMLLALLSFSVIGGVVTSISDLL
AAGVKALVDANLLALTSIIVEPAKILFLNNAINHGIFSPLGIQESSELGKSIFFLIEANPGPGLGILIAYMLFGKGSAKN
TASSASIIHFFGGIHEIYFPYVLMNPRLILAVIAGGATGVLTNTLLGGGLVSPASPGSIIAVLAMTPAGAHLAVVASVIL
SCAVTFVIASVLLKMQKDDGSEEDALESAQAEMAAMKAQSKGGVASVAGDFSNIRKIYVSCDAGMGSSAMGASMLRKKVQ
AAGLNIEVQNIAINDLPQNAQLVVTHKDLTARAQKQVPSAQHLSLNNFLDGAFYDSLVAKLNTNTAPVEKATQVETAPQA
ETASTGLNVTEAQIFLNLTARTKEEAIQFAGEQLVKLGFAEEDYIPAMFEREQQVSTYLGEGIAVPHGTIAAKDKVLKTG
IVFCQYREGVRFTDEEDGVAKLVIGIAARNNEHIQVVSAITNALDDEKAMEILTLTDDPQKVLRLLKGE

Sequences:

>Translated_629_residues
MSNLKVKVQSFGRFLSNMVMPNIGAFIAWGFITALFIPTGWLPNESFAKLVGPMISYLLPLLIGYSGGRLAGGERGAVVG
AITTMGIIVGSEIPMFLGAMIVGPLGGWVIKTFDKAIEGKVKSGFEMLVNNFSAGIIGMLLALLSFSVIGGVVTSISDLL
AAGVKALVDANLLALTSIIVEPAKILFLNNAINHGIFSPLGIQESSELGKSIFFLIEANPGPGLGILIAYMLFGKGSAKN
TASSASIIHFFGGIHEIYFPYVLMNPRLILAVIAGGATGVLTNTLLGGGLVSPASPGSIIAVLAMTPAGAHLAVVASVIL
SCAVTFVIASVLLKMQKDDGSEEDALESAQAEMAAMKAQSKGGVASVAGDFSNIRKIYVSCDAGMGSSAMGASMLRKKVQ
AAGLNIEVQNIAINDLPQNAQLVVTHKDLTARAQKQVPSAQHLSLNNFLDGAFYDSLVAKLNTNTAPVEKATQVETAPQA
ETASTGLNVTEAQIFLNLTARTKEEAIQFAGEQLVKLGFAEEDYIPAMFEREQQVSTYLGEGIAVPHGTIAAKDKVLKTG
IVFCQYREGVRFTDEEDGVAKLVIGIAARNNEHIQVVSAITNALDDEKAMEILTLTDDPQKVLRLLKGE
>Mature_628_residues
SNLKVKVQSFGRFLSNMVMPNIGAFIAWGFITALFIPTGWLPNESFAKLVGPMISYLLPLLIGYSGGRLAGGERGAVVGA
ITTMGIIVGSEIPMFLGAMIVGPLGGWVIKTFDKAIEGKVKSGFEMLVNNFSAGIIGMLLALLSFSVIGGVVTSISDLLA
AGVKALVDANLLALTSIIVEPAKILFLNNAINHGIFSPLGIQESSELGKSIFFLIEANPGPGLGILIAYMLFGKGSAKNT
ASSASIIHFFGGIHEIYFPYVLMNPRLILAVIAGGATGVLTNTLLGGGLVSPASPGSIIAVLAMTPAGAHLAVVASVILS
CAVTFVIASVLLKMQKDDGSEEDALESAQAEMAAMKAQSKGGVASVAGDFSNIRKIYVSCDAGMGSSAMGASMLRKKVQA
AGLNIEVQNIAINDLPQNAQLVVTHKDLTARAQKQVPSAQHLSLNNFLDGAFYDSLVAKLNTNTAPVEKATQVETAPQAE
TASTGLNVTEAQIFLNLTARTKEEAIQFAGEQLVKLGFAEEDYIPAMFEREQQVSTYLGEGIAVPHGTIAAKDKVLKTGI
VFCQYREGVRFTDEEDGVAKLVIGIAARNNEHIQVVSAITNALDDEKAMEILTLTDDPQKVLRLLKGE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2213

COG function: function code G; Phosphotransferase system, mannitol-specific IIBC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790027, Length=636, Percent_Identity=66.0377358490566, Blast_Score=822, Evalue=0.0,
Organism=Escherichia coli, GI1789301, Length=460, Percent_Identity=55.4347826086957, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI1788494, Length=127, Percent_Identity=43.3070866141732, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004718 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 66131; Mature: 66000

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLKVKVQSFGRFLSNMVMPNIGAFIAWGFITALFIPTGWLPNESFAKLVGPMISYLLP
CCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LLIGYSGGRLAGGERGAVVGAITTMGIIVGSEIPMFLGAMIVGPLGGWVIKTFDKAIEGK
HHHCCCCCCCCCCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCH
VKSGFEMLVNNFSAGIIGMLLALLSFSVIGGVVTSISDLLAAGVKALVDANLLALTSIIV
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EPAKILFLNNAINHGIFSPLGIQESSELGKSIFFLIEANPGPGLGILIAYMLFGKGSAKN
CCHHEEEEECHHHCCCCCCCCCCCHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCC
TASSASIIHFFGGIHEIYFPYVLMNPRLILAVIAGGATGVLTNTLLGGGLVSPASPGSII
CCCHHHHHHHHCCHHHHHHCHHEECCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEE
AVLAMTPAGAHLAVVASVILSCAVTFVIASVLLKMQKDDGSEEDALESAQAEMAAMKAQS
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
KGGVASVAGDFSNIRKIYVSCDAGMGSSAMGASMLRKKVQAAGLNIEVQNIAINDLPQNA
CCCEEHHHCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCC
QLVVTHKDLTARAQKQVPSAQHLSLNNFLDGAFYDSLVAKLNTNTAPVEKATQVETAPQA
EEEEEECCHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCC
ETASTGLNVTEAQIFLNLTARTKEEAIQFAGEQLVKLGFAEEDYIPAMFEREQQVSTYLG
CHHHCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
EGIAVPHGTIAAKDKVLKTGIVFCQYREGVRFTDEEDGVAKLVIGIAARNNEHIQVVSAI
CCCCCCCCCHHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHH
TNALDDEKAMEILTLTDDPQKVLRLLKGE
HHHCCCHHHEEEEEECCCHHHHHHHHCCC
>Mature Secondary Structure 
SNLKVKVQSFGRFLSNMVMPNIGAFIAWGFITALFIPTGWLPNESFAKLVGPMISYLLP
CCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LLIGYSGGRLAGGERGAVVGAITTMGIIVGSEIPMFLGAMIVGPLGGWVIKTFDKAIEGK
HHHCCCCCCCCCCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCH
VKSGFEMLVNNFSAGIIGMLLALLSFSVIGGVVTSISDLLAAGVKALVDANLLALTSIIV
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EPAKILFLNNAINHGIFSPLGIQESSELGKSIFFLIEANPGPGLGILIAYMLFGKGSAKN
CCHHEEEEECHHHCCCCCCCCCCCHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCC
TASSASIIHFFGGIHEIYFPYVLMNPRLILAVIAGGATGVLTNTLLGGGLVSPASPGSII
CCCHHHHHHHHCCHHHHHHCHHEECCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCEE
AVLAMTPAGAHLAVVASVILSCAVTFVIASVLLKMQKDDGSEEDALESAQAEMAAMKAQS
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
KGGVASVAGDFSNIRKIYVSCDAGMGSSAMGASMLRKKVQAAGLNIEVQNIAINDLPQNA
CCCEEHHHCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCC
QLVVTHKDLTARAQKQVPSAQHLSLNNFLDGAFYDSLVAKLNTNTAPVEKATQVETAPQA
EEEEEECCHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCC
ETASTGLNVTEAQIFLNLTARTKEEAIQFAGEQLVKLGFAEEDYIPAMFEREQQVSTYLG
CHHHCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
EGIAVPHGTIAAKDKVLKTGIVFCQYREGVRFTDEEDGVAKLVIGIAARNNEHIQVVSAI
CCCCCCCCCHHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHH
TNALDDEKAMEILTLTDDPQKVLRLLKGE
HHHCCCHHHEEEEEECCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]