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The map label for this gene is mrdA [H]

Identifier: 126209063

GI number: 126209063

Start: 1825881

End: 1827842

Strand: Reverse

Name: mrdA [H]

Synonym: APL_1599

Alternate gene names: 126209063

Gene position: 1827842-1825881 (Counterclockwise)

Preceding gene: 126209064

Following gene: 126209062

Centisome position: 80.36

GC content: 45.77

Gene sequence:

>1962_bases
ATGTTTGGTAAACTCGGAAAATTAACCAATCCAAAACCTCGTGAAAAAGTGCGGGACGGACAAGCGGAGAGCAATCTGTT
TGCCCGTCGTGCACTGGTCGCATTTCTTGGAGTATTGGTGCTTACGGGCGTGTTATTAACCAACCTGTATCATTTACAAG
TGGTAAATTATGACGACTACCAAACGCGTTCCAACGGGAACCGTATCAAACTATTACCTGTTCCGCCGACTCGCGGCTTG
ATCTACGACCGCAACGGTAAAGTATTAGCCGAAAACATTACCTATTTCGGGCTTTATATCATTCCTGAAAAAACCGAAAA
CCTAGAAGAAACGTTAGTTGAATTGCGTGATGTGGTCGGGCTGACCGACGAAGATGTGGAAGCGTTCCGCAAAGAGAAGA
AACGTACTTCTCGCTATACGCCGATTTTGTTAAAAGGCAATTTAAACGAGGAACAAATGGCGCGTTTTGCGGTAAATCAA
TATCGTTTCCCGAGTTTAGACGTACAGGCGTATTACAAACGTAATTATCCTTACGGCGAAACCTTGACCCATATTCTCGG
TTATGTGGCGAAAATCAACGAAAGAGATAAGAAAAAGCTCGAAGAAGAGGGTAAGTTCGGTAATTATGCCGGCTCGCACG
ATATGGGTAAATTGGGCATTGAGAAATATTATGAAGACCAATTACACGGACAAACCGGTTTTGAAGAGGTAGAAATCAAT
AACCGAGGTAAAGTGATTCGCAAATTACGTGATCAGCCGGGTGTAGCGGGCAGTAGTATTCGTTTAACCATTGATGTTGA
ATTGCAGCAATATATTCAGAATTTACTCGGTGATCAAAAAGGTTCGGTGGTGGTGCTTGACCCGCGTGACAGCAGCGTAT
TGGCGATGGTTACCAGCCCGAGCTACGACAATAATTTATTTGTCGGCGGCGTATCGGGAGCGGATTATAAACGCTTATTG
GAAAATCCGGCTCGTCCGTTATATAGTCGAGCGACACAGGGAACTTATCCGCCGGCTTCGACAGTGAAACCGTTTTATGC
GGTAATGGGATTGGCGGAAGGCAAAATTACGCCGCAAAATGCGATTTATGACCCGGGTTTTTGGATTTTACCGGGGACGA
CACAACGTTACCGAGATTGGAAAAAAGGCGGTCACGGTTCGACTAATGTGAATAAAGCGATTACCGAGTCTTCCGATACG
TTCTTCTATCAGCTCGCTTATGATACCGGTATCGATAAAATGTCGGAGTGGATGCGTAAATTCGGCTTCGGACAGAAAAC
GGGGATTGATATTTTTGAAGAATCCGCCGGTGTATATCCGAATCGTGAGTGGAAACAACGCCGTCATAAAAAACCGTGGG
TACAAGGTGATACGATTCCGGTAGGGATCGGTCAAGGTTACTGGATTGCAACACCGTTACAAGTGGCAAAAGCACAAGCG
ATCTTAATTAATAACGGTCGTGTAAACACGCCGCATTTGATGATGGAAGTCATCGGCAGTGATAAAACCGAGCCTTACAA
AGATCCGCTGTTATATGAAGATATTCAAGACGTGCCCGAGCGTTTTTGGCAAGTGGCGAAATTCGGAATGTACAACGTAA
TTAATGCGGCAAACGGTACGGGGCGCAAAGCCGGCGGTTCGTTTTATCGAGCGGCGGGTAAAAGCGGTACGGCGCAGGTG
TTTAACTTAAACGGCGGTAAGTACAATAAAAATGCGCTGAAAAAAGAGCTGCACGACCATGCGTGGTTTATCAGTTATGC
GCCGTATGAAGAGCCGAAAGTTTCGGTAGCGATTATTTTAGAAAATGCGGGCGGCGGCGGCTCTAATGCAGCGCCGGTGG
CTCGCCAAGTGATGGATTTTGCGTTAAGAAAAACCTTAAAAGAACCGATGGGAACATCAGAACCGTTGCAAAATATCGAG
CAAATTCAACCGCTTGAAGAGGGACAGGCAAATAATGAGTAG

Upstream 100 bases:

>100_bases
TCCTTATCATCGAGAATAGCTTGATCTAGAACGGACATGGTAAAAATGCCGAAATTTTGACCGCTTGTTCCGTTCGCTAG
AGAACAAAATGAGATAAACA

Downstream 100 bases:

>100_bases
CGGAATCAGAAAATTTTTTTGGAAGATTTTTTCCCTTGACGTATGGCTGTTGCTCGGCTTGTTGGCGATTACCGGTTACG
GTTTGATTGTGCTGTATAGT

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 653; Mature: 653

Protein sequence:

>653_residues
MFGKLGKLTNPKPREKVRDGQAESNLFARRALVAFLGVLVLTGVLLTNLYHLQVVNYDDYQTRSNGNRIKLLPVPPTRGL
IYDRNGKVLAENITYFGLYIIPEKTENLEETLVELRDVVGLTDEDVEAFRKEKKRTSRYTPILLKGNLNEEQMARFAVNQ
YRFPSLDVQAYYKRNYPYGETLTHILGYVAKINERDKKKLEEEGKFGNYAGSHDMGKLGIEKYYEDQLHGQTGFEEVEIN
NRGKVIRKLRDQPGVAGSSIRLTIDVELQQYIQNLLGDQKGSVVVLDPRDSSVLAMVTSPSYDNNLFVGGVSGADYKRLL
ENPARPLYSRATQGTYPPASTVKPFYAVMGLAEGKITPQNAIYDPGFWILPGTTQRYRDWKKGGHGSTNVNKAITESSDT
FFYQLAYDTGIDKMSEWMRKFGFGQKTGIDIFEESAGVYPNREWKQRRHKKPWVQGDTIPVGIGQGYWIATPLQVAKAQA
ILINNGRVNTPHLMMEVIGSDKTEPYKDPLLYEDIQDVPERFWQVAKFGMYNVINAANGTGRKAGGSFYRAAGKSGTAQV
FNLNGGKYNKNALKKELHDHAWFISYAPYEEPKVSVAIILENAGGGGSNAAPVARQVMDFALRKTLKEPMGTSEPLQNIE
QIQPLEEGQANNE

Sequences:

>Translated_653_residues
MFGKLGKLTNPKPREKVRDGQAESNLFARRALVAFLGVLVLTGVLLTNLYHLQVVNYDDYQTRSNGNRIKLLPVPPTRGL
IYDRNGKVLAENITYFGLYIIPEKTENLEETLVELRDVVGLTDEDVEAFRKEKKRTSRYTPILLKGNLNEEQMARFAVNQ
YRFPSLDVQAYYKRNYPYGETLTHILGYVAKINERDKKKLEEEGKFGNYAGSHDMGKLGIEKYYEDQLHGQTGFEEVEIN
NRGKVIRKLRDQPGVAGSSIRLTIDVELQQYIQNLLGDQKGSVVVLDPRDSSVLAMVTSPSYDNNLFVGGVSGADYKRLL
ENPARPLYSRATQGTYPPASTVKPFYAVMGLAEGKITPQNAIYDPGFWILPGTTQRYRDWKKGGHGSTNVNKAITESSDT
FFYQLAYDTGIDKMSEWMRKFGFGQKTGIDIFEESAGVYPNREWKQRRHKKPWVQGDTIPVGIGQGYWIATPLQVAKAQA
ILINNGRVNTPHLMMEVIGSDKTEPYKDPLLYEDIQDVPERFWQVAKFGMYNVINAANGTGRKAGGSFYRAAGKSGTAQV
FNLNGGKYNKNALKKELHDHAWFISYAPYEEPKVSVAIILENAGGGGSNAAPVARQVMDFALRKTLKEPMGTSEPLQNIE
QIQPLEEGQANNE
>Mature_653_residues
MFGKLGKLTNPKPREKVRDGQAESNLFARRALVAFLGVLVLTGVLLTNLYHLQVVNYDDYQTRSNGNRIKLLPVPPTRGL
IYDRNGKVLAENITYFGLYIIPEKTENLEETLVELRDVVGLTDEDVEAFRKEKKRTSRYTPILLKGNLNEEQMARFAVNQ
YRFPSLDVQAYYKRNYPYGETLTHILGYVAKINERDKKKLEEEGKFGNYAGSHDMGKLGIEKYYEDQLHGQTGFEEVEIN
NRGKVIRKLRDQPGVAGSSIRLTIDVELQQYIQNLLGDQKGSVVVLDPRDSSVLAMVTSPSYDNNLFVGGVSGADYKRLL
ENPARPLYSRATQGTYPPASTVKPFYAVMGLAEGKITPQNAIYDPGFWILPGTTQRYRDWKKGGHGSTNVNKAITESSDT
FFYQLAYDTGIDKMSEWMRKFGFGQKTGIDIFEESAGVYPNREWKQRRHKKPWVQGDTIPVGIGQGYWIATPLQVAKAQA
ILINNGRVNTPHLMMEVIGSDKTEPYKDPLLYEDIQDVPERFWQVAKFGMYNVINAANGTGRKAGGSFYRAAGKSGTAQV
FNLNGGKYNKNALKKELHDHAWFISYAPYEEPKVSVAIILENAGGGGSNAAPVARQVMDFALRKTLKEPMGTSEPLQNIE
QIQPLEEGQANNE

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=614, Percent_Identity=56.6775244299674, Blast_Score=720, Evalue=0.0,
Organism=Escherichia coli, GI1786272, Length=631, Percent_Identity=23.2963549920761, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 73377; Mature: 73377

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGKLGKLTNPKPREKVRDGQAESNLFARRALVAFLGVLVLTGVLLTNLYHLQVVNYDDY
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCC
QTRSNGNRIKLLPVPPTRGLIYDRNGKVLAENITYFGLYIIPEKTENLEETLVELRDVVG
CCCCCCCEEEEEECCCCCCEEECCCCCEEEECCEEEEEEEECCCCCCHHHHHHHHHHHHC
LTDEDVEAFRKEKKRTSRYTPILLKGNLNEEQMARFAVNQYRFPSLDVQAYYKRNYPYGE
CCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHHEECCCCCHH
TLTHILGYVAKINERDKKKLEEEGKFGNYAGSHDMGKLGIEKYYEDQLHGQTGFEEVEIN
HHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEC
NRGKVIRKLRDQPGVAGSSIRLTIDVELQQYIQNLLGDQKGSVVVLDPRDSSVLAMVTSP
CCHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEECC
SYDNNLFVGGVSGADYKRLLENPARPLYSRATQGTYPPASTVKPFYAVMGLAEGKITPQN
CCCCCEEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCCHHHCCHHHHHHHHCCCCCCCCC
AIYDPGFWILPGTTQRYRDWKKGGHGSTNVNKAITESSDTFFYQLAYDTGIDKMSEWMRK
CEECCCEEEECCCHHHHHHHHCCCCCCCCCHHHHCCCCCCEEEEEEECCCHHHHHHHHHH
FGFGQKTGIDIFEESAGVYPNREWKQRRHKKPWVQGDTIPVGIGQGYWIATPLQVAKAQA
HCCCCCCCCHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEEECCCCEEEECHHHHHCCEE
ILINNGRVNTPHLMMEVIGSDKTEPYKDPLLYEDIQDVPERFWQVAKFGMYNVINAANGT
EEEECCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCCC
GRKAGGSFYRAAGKSGTAQVFNLNGGKYNKNALKKELHDHAWFISYAPYEEPKVSVAIIL
CCCCCCCHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCEEEEEECCCCCCCEEEEEEE
ENAGGGGSNAAPVARQVMDFALRKTLKEPMGTSEPLQNIEQIQPLEEGQANNE
ECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCCCCC
>Mature Secondary Structure
MFGKLGKLTNPKPREKVRDGQAESNLFARRALVAFLGVLVLTGVLLTNLYHLQVVNYDDY
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCC
QTRSNGNRIKLLPVPPTRGLIYDRNGKVLAENITYFGLYIIPEKTENLEETLVELRDVVG
CCCCCCCEEEEEECCCCCCEEECCCCCEEEECCEEEEEEEECCCCCCHHHHHHHHHHHHC
LTDEDVEAFRKEKKRTSRYTPILLKGNLNEEQMARFAVNQYRFPSLDVQAYYKRNYPYGE
CCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHHEECCCCCHH
TLTHILGYVAKINERDKKKLEEEGKFGNYAGSHDMGKLGIEKYYEDQLHGQTGFEEVEIN
HHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEC
NRGKVIRKLRDQPGVAGSSIRLTIDVELQQYIQNLLGDQKGSVVVLDPRDSSVLAMVTSP
CCHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEECC
SYDNNLFVGGVSGADYKRLLENPARPLYSRATQGTYPPASTVKPFYAVMGLAEGKITPQN
CCCCCEEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCCHHHCCHHHHHHHHCCCCCCCCC
AIYDPGFWILPGTTQRYRDWKKGGHGSTNVNKAITESSDTFFYQLAYDTGIDKMSEWMRK
CEECCCEEEECCCHHHHHHHHCCCCCCCCCHHHHCCCCCCEEEEEEECCCHHHHHHHHHH
FGFGQKTGIDIFEESAGVYPNREWKQRRHKKPWVQGDTIPVGIGQGYWIATPLQVAKAQA
HCCCCCCCCHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEEECCCCEEEECHHHHHCCEE
ILINNGRVNTPHLMMEVIGSDKTEPYKDPLLYEDIQDVPERFWQVAKFGMYNVINAANGT
EEEECCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCCC
GRKAGGSFYRAAGKSGTAQVFNLNGGKYNKNALKKELHDHAWFISYAPYEEPKVSVAIIL
CCCCCCCHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCEEEEEECCCCCCCEEEEEEE
ENAGGGGSNAAPVARQVMDFALRKTLKEPMGTSEPLQNIEQIQPLEEGQANNE
ECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]