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The map label for this gene is rlmH

Identifier: 126209064

GI number: 126209064

Start: 1827924

End: 1828391

Strand: Reverse

Name: rlmH

Synonym: APL_1600

Alternate gene names: 126209064

Gene position: 1828391-1827924 (Counterclockwise)

Preceding gene: 126209065

Following gene: 126209063

Centisome position: 80.39

GC content: 48.93

Gene sequence:

>468_bases
ATGAAAATACAATTAATTGCGGTCGGGCAAAAAATGCCGGATTGGGTAAAGGTCGGTTTTGAAGAATACCAACGCCGTTT
CCCCAAAGATATGCCGTTTGAACTGATTGAGATTCCCGCCGGTAAACGCGGCAAAAATGCGGATATTAAACGCATTTTAG
AGCAAGAAGGCAAAGCGATGTTAGCCGCCGCCGGTAAAGGCAAAGTTGTGACCTTGGATATTCCGGGCAAGCCGTGGACA
ACCGAACAATTGGCGAGCCAATTGGAGGCGTGGAAAAATGACGGGCGAGACGTATGCTTATTGATCGGCGGGCCGGAAGG
ACTTTCGCCGGAATGTAAAGCCGCTGCGGAACAAAGCTGGTCTCTTTCGCCGCTAACCTTACCGCATCCGATGGTGCGCG
TAATTGTTGCTGAAAGTTTATATCGTGCGTGGTCACTTACGACAAATCATCCTTATCATCGAGAATAG

Upstream 100 bases:

>100_bases
GATTTCGGTCAAGCGATTGTGCATTTATTACAAGCGGATGCCCGTGAAATGTATCAATTAGAAAAACTGTGGGCATAATC
GTCATTTTCGTGGTGGCACA

Downstream 100 bases:

>100_bases
CTTGATCTAGAACGGACATGGTAAAAATGCCGAAATTTTGACCGCTTGTTCCGTTCGCTAGAGAACAAAATGAGATAAAC
AATGTTTGGTAAACTCGGAA

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MKIQLIAVGQKMPDWVKVGFEEYQRRFPKDMPFELIEIPAGKRGKNADIKRILEQEGKAMLAAAGKGKVVTLDIPGKPWT
TEQLASQLEAWKNDGRDVCLLIGGPEGLSPECKAAAEQSWSLSPLTLPHPMVRVIVAESLYRAWSLTTNHPYHRE

Sequences:

>Translated_155_residues
MKIQLIAVGQKMPDWVKVGFEEYQRRFPKDMPFELIEIPAGKRGKNADIKRILEQEGKAMLAAAGKGKVVTLDIPGKPWT
TEQLASQLEAWKNDGRDVCLLIGGPEGLSPECKAAAEQSWSLSPLTLPHPMVRVIVAESLYRAWSLTTNHPYHRE
>Mature_155_residues
MKIQLIAVGQKMPDWVKVGFEEYQRRFPKDMPFELIEIPAGKRGKNADIKRILEQEGKAMLAAAGKGKVVTLDIPGKPWT
TEQLASQLEAWKNDGRDVCLLIGGPEGLSPECKAAAEQSWSLSPLTLPHPMVRVIVAESLYRAWSLTTNHPYHRE

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=155, Percent_Identity=83.2258064516129, Blast_Score=274, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_ACTP2 (A3N2P8)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054289.1
- ProteinModelPortal:   A3N2P8
- SMR:   A3N2P8
- STRING:   A3N2P8
- GeneID:   4849303
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1600
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- PhylomeDB:   A3N2P8
- ProtClustDB:   PRK00103
- BioCyc:   APLE416269:APL_1600-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17347; Mature: 17347

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIQLIAVGQKMPDWVKVGFEEYQRRFPKDMPFELIEIPAGKRGKNADIKRILEQEGKAM
CEEEEEECCCCCCHHHHHCHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCEE
LAAAGKGKVVTLDIPGKPWTTEQLASQLEAWKNDGRDVCLLIGGPEGLSPECKAAAEQSW
EEECCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCC
SLSPLTLPHPMVRVIVAESLYRAWSLTTNHPYHRE
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKIQLIAVGQKMPDWVKVGFEEYQRRFPKDMPFELIEIPAGKRGKNADIKRILEQEGKAM
CEEEEEECCCCCCHHHHHCHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCEE
LAAAGKGKVVTLDIPGKPWTTEQLASQLEAWKNDGRDVCLLIGGPEGLSPECKAAAEQSW
EEECCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCC
SLSPLTLPHPMVRVIVAESLYRAWSLTTNHPYHRE
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA