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The map label for this gene is dlgD [H]

Identifier: 126209037

GI number: 126209037

Start: 1800111

End: 1800818

Strand: Reverse

Name: dlgD [H]

Synonym: APL_1573

Alternate gene names: 126209037

Gene position: 1800818-1800111 (Counterclockwise)

Preceding gene: 126209039

Following gene: 126209036

Centisome position: 79.17

GC content: 49.29

Gene sequence:

>708_bases
ATGGAGCTGGCTTCGCAAAACGGCATCGGTGTGGTGGCATTGCGTAACGCAAACCACTGGATGCGTGGCGGCTCTTACGG
CTGGCAAGCAGCGGAAAAAGGTTATATCGGTATCTGCTGGACAAACGCTTTAGCCGTTATGCCACCTTGGGGGGCGAAAG
AGTACCGTATCGGAACTAACCCGTTAATTGTTGCGGTGCCAACTACGCCAATTACCATGGTCGATATGTCTTGCTCAATG
TACTCCTACGGAATGCTCGAAGTGCACCGCTTAGCCGGTCGCCAAACTTTTGTTGATGCGGGTTTTGATGATGACGGTAA
CCCAACTCGTGATCCGGCAACCGTGGAGAAAAACCGCCGCTTAATGCCAATGGGCTTCTGGAAAGGTTCGGGCTTATCGA
TTGTGTTAGATATGATCGCAACCCTGCTTTCTAACGGTGAATCCACCGCTACGGTTACCGAAGATAAAGATGACGAATAC
TGCGTTTCCCAGGTGTTTATTGCAATTGAAGTCGATAGACTGATTGACGGCAAAACCAAAGACGAAAAACTTAACCGTAT
TATGGATTACGTTCGCACCGCAGAGCGTGCAGACCCAGAAGTGGCTGTACGTTTACCGGGCCACGAATTTACCCAAATTC
TGGCGGATAACAAAGCAAATGGTATTCCGGTAGATGACACCGTATGGGCGAAATTACAGTCGCTATAA

Upstream 100 bases:

>100_bases
GCAGTACCAACTAAAGTGCTTTCATTAGGTGCAATTGAGCAGTGGGACGCACACCAAGCCATCGGTAACTTAACTGCGAA
AAAAAATGATGGACAGAGCG

Downstream 100 bases:

>100_bases
TTTAGGACAGAGGCGGAGCCTCTGAGGAATGCGTAAACCCCACATGGCTCGTGTGGGGGACAACGTTTGTGCTTGTGTAT
TAGATTTGTCGATAATTAAA

Product: 2,3-diketo-L-gulonate reductase

Products: NA

Alternate protein names: 2,3-DKG reductase; 3-dehydro-L-gulonate 2-dehydrogenase [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MELASQNGIGVVALRNANHWMRGGSYGWQAAEKGYIGICWTNALAVMPPWGAKEYRIGTNPLIVAVPTTPITMVDMSCSM
YSYGMLEVHRLAGRQTFVDAGFDDDGNPTRDPATVEKNRRLMPMGFWKGSGLSIVLDMIATLLSNGESTATVTEDKDDEY
CVSQVFIAIEVDRLIDGKTKDEKLNRIMDYVRTAERADPEVAVRLPGHEFTQILADNKANGIPVDDTVWAKLQSL

Sequences:

>Translated_235_residues
MELASQNGIGVVALRNANHWMRGGSYGWQAAEKGYIGICWTNALAVMPPWGAKEYRIGTNPLIVAVPTTPITMVDMSCSM
YSYGMLEVHRLAGRQTFVDAGFDDDGNPTRDPATVEKNRRLMPMGFWKGSGLSIVLDMIATLLSNGESTATVTEDKDDEY
CVSQVFIAIEVDRLIDGKTKDEKLNRIMDYVRTAERADPEVAVRLPGHEFTQILADNKANGIPVDDTVWAKLQSL
>Mature_235_residues
MELASQNGIGVVALRNANHWMRGGSYGWQAAEKGYIGICWTNALAVMPPWGAKEYRIGTNPLIVAVPTTPITMVDMSCSM
YSYGMLEVHRLAGRQTFVDAGFDDDGNPTRDPATVEKNRRLMPMGFWKGSGLSIVLDMIATLLSNGESTATVTEDKDDEY
CVSQVFIAIEVDRLIDGKTKDEKLNRIMDYVRTAERADPEVAVRLPGHEFTQILADNKANGIPVDDTVWAKLQSL

Specific function: Catalyzes the reduction of 2,3-diketo-L-gulonate in the presence of NADH, to form 3-keto-L-gulonate [H]

COG id: COG2055

COG function: function code C; Malate/L-lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH2/MDH2 oxidoreductase family. DlgD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790000, Length=235, Percent_Identity=75.7446808510638, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI1787020, Length=242, Percent_Identity=24.7933884297521, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786727, Length=241, Percent_Identity=26.1410788381743, Blast_Score=68, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17507179, Length=223, Percent_Identity=26.9058295964126, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI45553211, Length=226, Percent_Identity=27.4336283185841, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24667912, Length=226, Percent_Identity=27.4336283185841, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24667908, Length=226, Percent_Identity=27.4336283185841, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24667904, Length=226, Percent_Identity=27.4336283185841, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003767 [H]

Pfam domain/function: PF02615 Ldh_2 [H]

EC number: =1.1.1.130 [H]

Molecular weight: Translated: 25933; Mature: 25933

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELASQNGIGVVALRNANHWMRGGSYGWQAAEKGYIGICWTNALAVMPPWGAKEYRIGTN
CCCCCCCCEEEEEEECCCCCCCCCCCCCHHCCCCEEEEEECCEEEEECCCCCCCEEECCC
PLIVAVPTTPITMVDMSCSMYSYGMLEVHRLAGRQTFVDAGFDDDGNPTRDPATVEKNRR
CEEEEECCCCEEEEECCCCHHHCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHCCCE
LMPMGFWKGSGLSIVLDMIATLLSNGESTATVTEDKDDEYCVSQVFIAIEVDRLIDGKTK
EEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHEEEEEEEEHHHCCCCH
DEKLNRIMDYVRTAERADPEVAVRLPGHEFTQILADNKANGIPVDDTVWAKLQSL
HHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MELASQNGIGVVALRNANHWMRGGSYGWQAAEKGYIGICWTNALAVMPPWGAKEYRIGTN
CCCCCCCCEEEEEEECCCCCCCCCCCCCHHCCCCEEEEEECCEEEEECCCCCCCEEECCC
PLIVAVPTTPITMVDMSCSMYSYGMLEVHRLAGRQTFVDAGFDDDGNPTRDPATVEKNRR
CEEEEECCCCEEEEECCCCHHHCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHCCCE
LMPMGFWKGSGLSIVLDMIATLLSNGESTATVTEDKDDEYCVSQVFIAIEVDRLIDGKTK
EEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHEEEEEEEEHHHCCCCH
DEKLNRIMDYVRTAERADPEVAVRLPGHEFTQILADNKANGIPVDDTVWAKLQSL
HHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA