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The map label for this gene is hybA [H]

Identifier: 126208796

GI number: 126208796

Start: 1528872

End: 1529909

Strand: Direct

Name: hybA [H]

Synonym: APL_1332

Alternate gene names: 126208796

Gene position: 1528872-1529909 (Clockwise)

Preceding gene: 126208795

Following gene: 126208797

Centisome position: 67.22

GC content: 44.03

Gene sequence:

>1038_bases
ATGAATAGACGTAAGTTTCTAAAAGTAGGCATCGGCGGCGGATTAGCTTCCGGTCTTACACCGAGCGTTGCTAACGCTCA
AGCGGAAAATCGTCCTCGGATACCGACTGCCGTCGGTATGTTGTACGATTCTACGCTTTGTGTAGGTTGCCAAGCTTGTG
TCGCCGAATGTCAGAATTTAAATAAAACGGCGATTAATCCTGTCGGAGAGCAAACTTGGTCGAATAACGATAAACTATCG
CCTTTCACACGAAACATTATTCAAGTTTGGTCAAACGGGGAAGGTAAATATAAGGATCAAGTCGATAACGGTTACGCTTA
CATTAAAAAACAATGTATGCACTGTGTTGAACCCAATTGCGTAACAGCCTGTCCGGTGCAAGCGCTGACTAAAGACGAAA
AAACCGGTATCGTTAATTATGATCCCGATATTTGTACCGGTTGTCGTTATTGTATGGTTGCCTGTCCGTTTAACGTACCT
AAATACGACTATAACAACCCGTTCGGCGAAATCAGCAAATGCGAACTTTGTAACCAAAAAGGATTGGAACGTATTGATAA
GGGCGAGAATCCGGGCTGCTGTCAGGTTTGTCCGACCGGCGCAATTATTTTCGGTAGCTATAACGACTTATTAGCCGAAG
CTAAACGCCGTCTGACGTTACTGAGAGGAACGGAGTATGACTACCCTCGTCAAACGGTAGATAGTAAAGATACTTATAAG
GCTAAAGTACCTCTATATCAACAACATATTTACGGAGAAATCGAAGGCGGCGGTACGCAAGTATTGGTACTTAGCGGCGT
TCCGCATGAAAATTTAGATTTACCTCAGTTGGACGAACTGGCGACAGGTACGCGAGCGGCACATTTACAACATACCTTAT
ATAAAGGCATGATCTTACCGCTAGTCGCTTTGGCGGGTTTAACCGCAATGACTTATAGAAGTCTGCATGCGGATAAAATC
GAAGCTAAAAAACAGGAATGGAAATTAGCTCGCGAACAGATGAAAGTAGAAGAAGAGAAGGAGGATCACCATGGCTAA

Upstream 100 bases:

>100_bases
GCCGGGGTACTGACCGGGGTGAGTGTGATGACGTTACGCAGTTTGAGTATTCAACATAAAGCTCAACAACAAGCTTCAAA
TAATTCTCGGGAACAATATC

Downstream 100 bases:

>100_bases
AGCTCGTCCTCTCGGCGGTCGTTTAATATCAATCCCCGTATTGGTATTCGCACCGCTTGCCGCTATTTGCGCTTTATTAA
TATTACGTCGTCTGATTTTC

Product: hydrogenase 2 protein HybA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MNRRKFLKVGIGGGLASGLTPSVANAQAENRPRIPTAVGMLYDSTLCVGCQACVAECQNLNKTAINPVGEQTWSNNDKLS
PFTRNIIQVWSNGEGKYKDQVDNGYAYIKKQCMHCVEPNCVTACPVQALTKDEKTGIVNYDPDICTGCRYCMVACPFNVP
KYDYNNPFGEISKCELCNQKGLERIDKGENPGCCQVCPTGAIIFGSYNDLLAEAKRRLTLLRGTEYDYPRQTVDSKDTYK
AKVPLYQQHIYGEIEGGGTQVLVLSGVPHENLDLPQLDELATGTRAAHLQHTLYKGMILPLVALAGLTAMTYRSLHADKI
EAKKQEWKLAREQMKVEEEKEDHHG

Sequences:

>Translated_345_residues
MNRRKFLKVGIGGGLASGLTPSVANAQAENRPRIPTAVGMLYDSTLCVGCQACVAECQNLNKTAINPVGEQTWSNNDKLS
PFTRNIIQVWSNGEGKYKDQVDNGYAYIKKQCMHCVEPNCVTACPVQALTKDEKTGIVNYDPDICTGCRYCMVACPFNVP
KYDYNNPFGEISKCELCNQKGLERIDKGENPGCCQVCPTGAIIFGSYNDLLAEAKRRLTLLRGTEYDYPRQTVDSKDTYK
AKVPLYQQHIYGEIEGGGTQVLVLSGVPHENLDLPQLDELATGTRAAHLQHTLYKGMILPLVALAGLTAMTYRSLHADKI
EAKKQEWKLAREQMKVEEEKEDHHG
>Mature_345_residues
MNRRKFLKVGIGGGLASGLTPSVANAQAENRPRIPTAVGMLYDSTLCVGCQACVAECQNLNKTAINPVGEQTWSNNDKLS
PFTRNIIQVWSNGEGKYKDQVDNGYAYIKKQCMHCVEPNCVTACPVQALTKDEKTGIVNYDPDICTGCRYCMVACPFNVP
KYDYNNPFGEISKCELCNQKGLERIDKGENPGCCQVCPTGAIIFGSYNDLLAEAKRRLTLLRGTEYDYPRQTVDSKDTYK
AKVPLYQQHIYGEIEGGGTQVLVLSGVPHENLDLPQLDELATGTRAAHLQHTLYKGMILPLVALAGLTAMTYRSLHADKI
EAKKQEWKLAREQMKVEEEKEDHHG

Specific function: Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=318, Percent_Identity=68.5534591194968, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1787749, Length=312, Percent_Identity=26.9230769230769, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790326, Length=332, Percent_Identity=27.1084337349398, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI2367345, Length=218, Percent_Identity=30.2752293577982, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787122, Length=178, Percent_Identity=31.4606741573034, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI226510944, Length=210, Percent_Identity=29.5238095238095, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1787872, Length=178, Percent_Identity=32.5842696629214, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787740, Length=99, Percent_Identity=36.3636363636364, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787478, Length=99, Percent_Identity=36.3636363636364, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082180, Length=156, Percent_Identity=32.0512820512821, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787963, Length=221, Percent_Identity=26.6968325791855, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 38245; Mature: 38245

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRKFLKVGIGGGLASGLTPSVANAQAENRPRIPTAVGMLYDSTLCVGCQACVAECQNL
CCCCEEEEEECCCCHHCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
NKTAINPVGEQTWSNNDKLSPFTRNIIQVWSNGEGKYKDQVDNGYAYIKKQCMHCVEPNC
CCHHCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHCCCCC
VTACPVQALTKDEKTGIVNYDPDICTGCRYCMVACPFNVPKYDYNNPFGEISKCELCNQK
CCCCCHHHHCCCCCCCCEECCCCHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHH
GLERIDKGENPGCCQVCPTGAIIFGSYNDLLAEAKRRLTLLRGTEYDYPRQTVDSKDTYK
HHHHHHCCCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
AKVPLYQQHIYGEIEGGGTQVLVLSGVPHENLDLPQLDELATGTRAAHLQHTLYKGMILP
EECCHHHHHHCEEECCCCEEEEEEECCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHH
LVALAGLTAMTYRSLHADKIEAKKQEWKLAREQMKVEEEKEDHHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNRRKFLKVGIGGGLASGLTPSVANAQAENRPRIPTAVGMLYDSTLCVGCQACVAECQNL
CCCCEEEEEECCCCHHCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
NKTAINPVGEQTWSNNDKLSPFTRNIIQVWSNGEGKYKDQVDNGYAYIKKQCMHCVEPNC
CCHHCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHCCCCC
VTACPVQALTKDEKTGIVNYDPDICTGCRYCMVACPFNVPKYDYNNPFGEISKCELCNQK
CCCCCHHHHCCCCCCCCEECCCCHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHH
GLERIDKGENPGCCQVCPTGAIIFGSYNDLLAEAKRRLTLLRGTEYDYPRQTVDSKDTYK
HHHHHHCCCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
AKVPLYQQHIYGEIEGGGTQVLVLSGVPHENLDLPQLDELATGTRAAHLQHTLYKGMILP
EECCHHHHHHCEEECCCCEEEEEEECCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHH
LVALAGLTAMTYRSLHADKIEAKKQEWKLAREQMKVEEEKEDHHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]