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The map label for this gene is hyaA [H]

Identifier: 126208795

GI number: 126208795

Start: 1527737

End: 1528879

Strand: Direct

Name: hyaA [H]

Synonym: APL_1331

Alternate gene names: 126208795

Gene position: 1527737-1528879 (Clockwise)

Preceding gene: 126208793

Following gene: 126208796

Centisome position: 67.17

GC content: 45.23

Gene sequence:

>1143_bases
ATGCAACGATATGATGACCTGTTTTCTGCCCTTACCGATGTTTCTCGTCGGGATTTTATGAAATTATGTACCGCCTTAGC
CGCAACGATGGGGTTAAATGCTAAAGCAAGCGCAGAAATGACTAATGCCTTAACCAGCCCGCAACGCCCTCCGGTAATTT
GGATTGGCGCGCAAGAATGTACCGGTTGTACGGAATCTTTATTACGTGCGACGCACCCTACGGTAGAAAACCTTGTATTG
GATTTGATTTCTCTTGAATATCACGAAGTGCTATCGGCGGCTTTCGGTGACCAAGCGGAAGATAATAAACATAATGCAAT
ACATAAATATAAAGGTCAGTATATTTTAGTCGTGGACGGTTCTATTCCGGTTAAAGACGACGGTATATATTGTATGATTG
CGGGTAAACCCGTTTTAGAACATATCAAAGAAGCCGCTAAAGATGCGATGGCAATCATTGCTATCGGTTCGTGCGCTGCG
TGGGGCGGCGTTCCTTCAAGCGGTAATAATCCGACCGGTGCAAGCAGTTTATCCGATATTTTACCGGGTATGCCGATTAT
TAATATCCCGGGTTGTCCTCCTAATCCTCATAATTTCTTGGCGACCGTCGCCTATATTATTACCTATAAAAAATTACCGA
ATATGGACAAACTTAATCGTCCGTTATTCGCTTACGACAGACTTATTCACGAAAACTGTTATAGAAGACCGCACTTTGAT
GCGGGAAGATTTGCACGCGAATACGGCGATGAAGGACACCGACAAGGTTGGTGTTTATACCATTTAGGCTGTAAATGTCC
GGAAACTTACGGTAACTGTTCAACCCTTGAATTCTGCGATGTCGGCGGTAATAACTGGCCGGTCGGTATCGGTCATCCTT
GCTACGGCTGCAATGAACAAGGCGTCGGATTTACCAAAGGCATTTTCCAATTGGCTACGGTGGAGAACCCGACACCAAGA
GTGGATAAGCCGAGTGTAAATATCACGGAAGGAAATCCCGCCTCAAAAACCGTTATCGGTTTACTCGGCGGAGCCGCCGG
GGTACTGACCGGGGTGAGTGTGATGACGTTACGCAGTTTGAGTATTCAACATAAAGCTCAACAACAAGCTTCAAATAATT
CTCGGGAACAATATCATGAATAG

Upstream 100 bases:

>100_bases
TTTCTTAAAAACCTTAATTTTTATGAGATAAATCAAATTTATTGCATATTAAAGAGCCTATGATTAGCCCATATATAAAG
TGTGTATTTAGGAGTGAAAT

Downstream 100 bases:

>100_bases
ACGTAAGTTTCTAAAAGTAGGCATCGGCGGCGGATTAGCTTCCGGTCTTACACCGAGCGTTGCTAACGCTCAAGCGGAAA
ATCGTCCTCGGATACCGACT

Product: hydrogenase 2 small subunit

Products: NA

Alternate protein names: HYD2; Membrane-bound hydrogenase 2 small subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MQRYDDLFSALTDVSRRDFMKLCTALAATMGLNAKASAEMTNALTSPQRPPVIWIGAQECTGCTESLLRATHPTVENLVL
DLISLEYHEVLSAAFGDQAEDNKHNAIHKYKGQYILVVDGSIPVKDDGIYCMIAGKPVLEHIKEAAKDAMAIIAIGSCAA
WGGVPSSGNNPTGASSLSDILPGMPIINIPGCPPNPHNFLATVAYIITYKKLPNMDKLNRPLFAYDRLIHENCYRRPHFD
AGRFAREYGDEGHRQGWCLYHLGCKCPETYGNCSTLEFCDVGGNNWPVGIGHPCYGCNEQGVGFTKGIFQLATVENPTPR
VDKPSVNITEGNPASKTVIGLLGGAAGVLTGVSVMTLRSLSIQHKAQQQASNNSREQYHE

Sequences:

>Translated_380_residues
MQRYDDLFSALTDVSRRDFMKLCTALAATMGLNAKASAEMTNALTSPQRPPVIWIGAQECTGCTESLLRATHPTVENLVL
DLISLEYHEVLSAAFGDQAEDNKHNAIHKYKGQYILVVDGSIPVKDDGIYCMIAGKPVLEHIKEAAKDAMAIIAIGSCAA
WGGVPSSGNNPTGASSLSDILPGMPIINIPGCPPNPHNFLATVAYIITYKKLPNMDKLNRPLFAYDRLIHENCYRRPHFD
AGRFAREYGDEGHRQGWCLYHLGCKCPETYGNCSTLEFCDVGGNNWPVGIGHPCYGCNEQGVGFTKGIFQLATVENPTPR
VDKPSVNITEGNPASKTVIGLLGGAAGVLTGVSVMTLRSLSIQHKAQQQASNNSREQYHE
>Mature_380_residues
MQRYDDLFSALTDVSRRDFMKLCTALAATMGLNAKASAEMTNALTSPQRPPVIWIGAQECTGCTESLLRATHPTVENLVL
DLISLEYHEVLSAAFGDQAEDNKHNAIHKYKGQYILVVDGSIPVKDDGIYCMIAGKPVLEHIKEAAKDAMAIIAIGSCAA
WGGVPSSGNNPTGASSLSDILPGMPIINIPGCPPNPHNFLATVAYIITYKKLPNMDKLNRPLFAYDRLIHENCYRRPHFD
AGRFAREYGDEGHRQGWCLYHLGCKCPETYGNCSTLEFCDVGGNNWPVGIGHPCYGCNEQGVGFTKGIFQLATVENPTPR
VDKPSVNITEGNPASKTVIGLLGGAAGVLTGVSVMTLRSLSIQHKAQQQASNNSREQYHE

Specific function: This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake [H]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Periplasmic side. Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=358, Percent_Identity=73.463687150838, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI1787206, Length=304, Percent_Identity=43.0921052631579, Blast_Score=271, Evalue=5e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 41238; Mature: 41238

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRYDDLFSALTDVSRRDFMKLCTALAATMGLNAKASAEMTNALTSPQRPPVIWIGAQEC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEECHHH
TGCTESLLRATHPTVENLVLDLISLEYHEVLSAAFGDQAEDNKHNAIHKYKGQYILVVDG
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEECCCEEEEECC
SIPVKDDGIYCMIAGKPVLEHIKEAAKDAMAIIAIGSCAAWGGVPSSGNNPTGASSLSDI
CCCCCCCCEEEEEECCHHHHHHHHHHHHHEEEEEECCHHHCCCCCCCCCCCCCHHHHHHH
LPGMPIINIPGCPPNPHNFLATVAYIITYKKLPNMDKLNRPLFAYDRLIHENCYRRPHFD
HCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHCCCCCC
AGRFAREYGDEGHRQGWCLYHLGCKCPETYGNCSTLEFCDVGGNNWPVGIGHPCYGCNEQ
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
GVGFTKGIFQLATVENPTPRVDKPSVNITEGNPASKTVIGLLGGAAGVLTGVSVMTLRSL
CCCHHHHHHHEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SIQHKAQQQASNNSREQYHE
HHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure
MQRYDDLFSALTDVSRRDFMKLCTALAATMGLNAKASAEMTNALTSPQRPPVIWIGAQEC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEECHHH
TGCTESLLRATHPTVENLVLDLISLEYHEVLSAAFGDQAEDNKHNAIHKYKGQYILVVDG
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEECCCEEEEECC
SIPVKDDGIYCMIAGKPVLEHIKEAAKDAMAIIAIGSCAAWGGVPSSGNNPTGASSLSDI
CCCCCCCCEEEEEECCHHHHHHHHHHHHHEEEEEECCHHHCCCCCCCCCCCCCHHHHHHH
LPGMPIINIPGCPPNPHNFLATVAYIITYKKLPNMDKLNRPLFAYDRLIHENCYRRPHFD
HCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHCCCCCC
AGRFAREYGDEGHRQGWCLYHLGCKCPETYGNCSTLEFCDVGGNNWPVGIGHPCYGCNEQ
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
GVGFTKGIFQLATVENPTPRVDKPSVNITEGNPASKTVIGLLGGAAGVLTGVSVMTLRSL
CCCHHHHHHHEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SIQHKAQQQASNNSREQYHE
HHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]