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The map label for this gene is nrfX [H]

Identifier: 126208523

GI number: 126208523

Start: 1219936

End: 1220469

Strand: Reverse

Name: nrfX [H]

Synonym: APL_1051

Alternate gene names: 126208523

Gene position: 1220469-1219936 (Counterclockwise)

Preceding gene: 126208524

Following gene: 126208522

Centisome position: 53.66

GC content: 39.51

Gene sequence:

>534_bases
ATGAAAAAAACCTTTTTATTTTTACCTTTATTATTGTTGATTGCACTGGTAGCGTTTTTAACCGTACCGTTAATGAATAA
GGATGCCCTTTCGCCGACCGAAGATTGGCGAGATAAACCTTTTCCGGAATTTGTCGGTAAAAATTTACTCGAGCATAATG
CGCATATTAATAATAACAGTTTGCCGAAAGAGCCTTATATTTTAAACGTGTGGGCAAGTTGGTGCACTTGGTGTATTAAA
GAATTTCCGATTTTACTCAAATTGAAAGAACAAGGCGTGCCGATTGTCGGGCTTACCTATTCCGATCAACCGAAGGATGC
GCGCGAAGCCTTGGCTCGTTGGGGCAATCCGTTCGATTTGGTTATTGATGATTATGAAAAAGGGTTTCTAATTCAGACGC
TGAAAGTTTCGTCAGCACCTTCCAGTTATTTAATTGATAAACACGGTGTGGTACGTTACCAACAAAAAGGATATAACCCG
GATTTCGAGCAGGATTTTCTCCCTCGTTTAAAAGCGTTACAAGAGGAAAAATAA

Upstream 100 bases:

>100_bases
GTCGGCGCATTATGGCTTGGCGGTATTATTATGGTGATAGCTTCCATACTTGCCTTATTCGGTTATCGTCGAAAATCGGA
TCTTACAGCGGATAAATAAT

Downstream 100 bases:

>100_bases
TGACGAAACTATTGCAAAAAATCGCGTTTATTTTACCGCTTGTCTTCAGTTTGGTTGCTAAAGCGGAAATGGTGGATACT
TTTCAGTTTCAGAACGAAAC

Product: disulfide bound formation protein E (dsbE)

Products: NA

Alternate protein names: Cytochrome c biogenesis protein NrfX [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MKKTFLFLPLLLLIALVAFLTVPLMNKDALSPTEDWRDKPFPEFVGKNLLEHNAHINNNSLPKEPYILNVWASWCTWCIK
EFPILLKLKEQGVPIVGLTYSDQPKDAREALARWGNPFDLVIDDYEKGFLIQTLKVSSAPSSYLIDKHGVVRYQQKGYNP
DFEQDFLPRLKALQEEK

Sequences:

>Translated_177_residues
MKKTFLFLPLLLLIALVAFLTVPLMNKDALSPTEDWRDKPFPEFVGKNLLEHNAHINNNSLPKEPYILNVWASWCTWCIK
EFPILLKLKEQGVPIVGLTYSDQPKDAREALARWGNPFDLVIDDYEKGFLIQTLKVSSAPSSYLIDKHGVVRYQQKGYNP
DFEQDFLPRLKALQEEK
>Mature_177_residues
MKKTFLFLPLLLLIALVAFLTVPLMNKDALSPTEDWRDKPFPEFVGKNLLEHNAHINNNSLPKEPYILNVWASWCTWCIK
EFPILLKLKEQGVPIVGLTYSDQPKDAREALARWGNPFDLVIDDYEKGFLIQTLKVSSAPSSYLIDKHGVVRYQQKGYNP
DFEQDFLPRLKALQEEK

Specific function: Could be involved in disulfide bond formation. Could catalyzes a late, reductive step in the assembly of periplasmic NrfA c-type cytochrome, probably the reduction of disulfide bonds of the apocytochrome c to allow covalent linkage with the heme. Possible

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1788523, Length=162, Percent_Identity=34.5679012345679, Blast_Score=88, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004799
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF08534 Redoxin [H]

EC number: NA

Molecular weight: Translated: 20447; Mature: 20447

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTFLFLPLLLLIALVAFLTVPLMNKDALSPTEDWRDKPFPEFVGKNLLEHNAHINNNS
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCC
LPKEPYILNVWASWCTWCIKEFPILLKLKEQGVPIVGLTYSDQPKDAREALARWGNPFDL
CCCCCEEEEHHHHHHHHHHHHCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCCCEEE
VIDDYEKGFLIQTLKVSSAPSSYLIDKHGVVRYQQKGYNPDFEQDFLPRLKALQEEK
EEECCCCCEEEEEEECCCCCCCCEECCCCCEEECCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKTFLFLPLLLLIALVAFLTVPLMNKDALSPTEDWRDKPFPEFVGKNLLEHNAHINNNS
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCC
LPKEPYILNVWASWCTWCIKEFPILLKLKEQGVPIVGLTYSDQPKDAREALARWGNPFDL
CCCCCEEEEHHHHHHHHHHHHCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCCCEEE
VIDDYEKGFLIQTLKVSSAPSSYLIDKHGVVRYQQKGYNPDFEQDFLPRLKALQEEK
EEECCCCCEEEEEEECCCCCCCCEECCCCCEEECCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]