Click here to switch to the map view.

The map label for this gene is oppA [C]

Identifier: 126208475

GI number: 126208475

Start: 1163506

End: 1165056

Strand: Reverse

Name: oppA [C]

Synonym: APL_1001

Alternate gene names: 126208475

Gene position: 1165056-1163506 (Counterclockwise)

Preceding gene: 126208476

Following gene: 126208474

Centisome position: 51.22

GC content: 38.88

Gene sequence:

>1551_bases
ATGCACTCTGCGAAACAAGCGGTCAAATTTTTCCAAAATCTTGTCGGTTTATTAGCTTGTTTGCTTATATTATCCGCCTG
TGAGCGGGATTTGCCGGTCAAGCATGTTGAGCCGGCTAAACCCGAAACACTTCCGATCATAGACCATAATATTTTAAAAC
GAGCGATTTACACGAATAAATTTCAATTGGATCCGCATTTTGTTTATTCGTCGGCGGAATCGGCTCCGTTACGAGATCTA
TTTAGCGGCTTATTGGCTTATAATGAAAAAGGTATCGCTCAACCGGCATTGGCGAAAACATGGTTTACCGAAAATAATAA
AGACTGGTTGTTTATTTTAGATGAAAATGCAAAATGGTCTAACGGTAAAGCGGTTACCGCACAAGATATCGTGTTAAGCT
GGCAACGTTTAGCTAATCCAAAGAATGCCTCACCGTTAGCGCCGTATTTAGTTTATATGCAGTTAAAACACGCTAAAGAA
ATACTGAACGAAGAGAAAGCGGTCGGTGAATTGGGCGTTAAAGCGTTGAATGCGACCACATTACAAATTCGCTTGGAAAA
ACCGAATTTGGTTTTACCCAAAATGTTGGCACACGTTGCTTTATTGCCGAGTTATCAGGGAGAGATACCGAATCCTAATA
TGCTGATTACGAATGGCGATTATGCATTAGCCGCTCAAACGGACAAAAGTTTATTACTGCAAGCGGTTAAAAATAATCCT
TCTTTTGCCAAAGTGGAATATCATCGTATCTCGGTTTTACAATCGATTAAACCGTTCGATATAGTGGAGAATCCGTTAGA
AGGACAACAGCATAATATTTGGGAATTCCCTCGGTTATGTAATTATTATTATGAGTTTAATTTTGCCGATCCTCAGTTAA
AGCATAAAGAAATTCGCCAGGCAATAAAAACGATGATTTTGTCCGCCCGTATTCCACATCAATACGGTATTGCGAATTTT
TCGGTGTTACCGAAGAGTATGTTAAATTCGACTGAGCATCAGTGGAACCCGATTATTATTGAAACTTTATTGACACAAGC
GGGAATCAATTCAAATAACCCGTTACAGATTACGCTGAGTTATGACGAACAGGGCAAACATACGGAAATCGCTCAGCAAA
TGATTAGAACGTTAGGGCAATCGGATTTAATTAAAGTTACCCCTCAAGCACTCGATTGGTCGCAACTGTTAGCGGTAAGA
GAACAAAAAAATTATCAGTTAAGCAGAGCCGGATGGTGTGCCGAATATGCCGATCCGTTACCGTTTCTATTACATTTTCA
TTCTAAAAGCGTTGATAACAAAAGCAATTATCATAATCCGCAAGTAGATGAAAAGTTGGAAAGGTTACAAAATCAAAATT
TAACGGAAGAAGTAAGAACACAATTGATTCAGAGTGTCGTTAAACACCTTGATGACGATGTCGCCGTACTTCCGATGTTC
CAATACTATCGCCGTGTTGCATTAGATCCCACCATTTTAGGTATTGATTTAAATAACGATAGCGAAGTAATTTATAGTAA
ACATTTATATCGAATGAAGCCCAAGGATTAA

Upstream 100 bases:

>100_bases
TGGGGCTGATCCTCATGGCTTTAGGTATAGAAATTATTGTAGCTGGGCTTAGTAATTTATTTCCGGGATTAACTTCCGTT
CATTAGAAGGATAGAATGAG

Downstream 100 bases:

>100_bases
TTGATGAATCAACTTAACGAACAGCAATTAAGTGAAATTAAATTCGTGTTACAAAATTTCACTCACCCTACTTTACAAAA
GGACTTAATTGCATTGAATG

Product: putative binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MHSAKQAVKFFQNLVGLLACLLILSACERDLPVKHVEPAKPETLPIIDHNILKRAIYTNKFQLDPHFVYSSAESAPLRDL
FSGLLAYNEKGIAQPALAKTWFTENNKDWLFILDENAKWSNGKAVTAQDIVLSWQRLANPKNASPLAPYLVYMQLKHAKE
ILNEEKAVGELGVKALNATTLQIRLEKPNLVLPKMLAHVALLPSYQGEIPNPNMLITNGDYALAAQTDKSLLLQAVKNNP
SFAKVEYHRISVLQSIKPFDIVENPLEGQQHNIWEFPRLCNYYYEFNFADPQLKHKEIRQAIKTMILSARIPHQYGIANF
SVLPKSMLNSTEHQWNPIIIETLLTQAGINSNNPLQITLSYDEQGKHTEIAQQMIRTLGQSDLIKVTPQALDWSQLLAVR
EQKNYQLSRAGWCAEYADPLPFLLHFHSKSVDNKSNYHNPQVDEKLERLQNQNLTEEVRTQLIQSVVKHLDDDVAVLPMF
QYYRRVALDPTILGIDLNNDSEVIYSKHLYRMKPKD

Sequences:

>Translated_516_residues
MHSAKQAVKFFQNLVGLLACLLILSACERDLPVKHVEPAKPETLPIIDHNILKRAIYTNKFQLDPHFVYSSAESAPLRDL
FSGLLAYNEKGIAQPALAKTWFTENNKDWLFILDENAKWSNGKAVTAQDIVLSWQRLANPKNASPLAPYLVYMQLKHAKE
ILNEEKAVGELGVKALNATTLQIRLEKPNLVLPKMLAHVALLPSYQGEIPNPNMLITNGDYALAAQTDKSLLLQAVKNNP
SFAKVEYHRISVLQSIKPFDIVENPLEGQQHNIWEFPRLCNYYYEFNFADPQLKHKEIRQAIKTMILSARIPHQYGIANF
SVLPKSMLNSTEHQWNPIIIETLLTQAGINSNNPLQITLSYDEQGKHTEIAQQMIRTLGQSDLIKVTPQALDWSQLLAVR
EQKNYQLSRAGWCAEYADPLPFLLHFHSKSVDNKSNYHNPQVDEKLERLQNQNLTEEVRTQLIQSVVKHLDDDVAVLPMF
QYYRRVALDPTILGIDLNNDSEVIYSKHLYRMKPKD
>Mature_516_residues
MHSAKQAVKFFQNLVGLLACLLILSACERDLPVKHVEPAKPETLPIIDHNILKRAIYTNKFQLDPHFVYSSAESAPLRDL
FSGLLAYNEKGIAQPALAKTWFTENNKDWLFILDENAKWSNGKAVTAQDIVLSWQRLANPKNASPLAPYLVYMQLKHAKE
ILNEEKAVGELGVKALNATTLQIRLEKPNLVLPKMLAHVALLPSYQGEIPNPNMLITNGDYALAAQTDKSLLLQAVKNNP
SFAKVEYHRISVLQSIKPFDIVENPLEGQQHNIWEFPRLCNYYYEFNFADPQLKHKEIRQAIKTMILSARIPHQYGIANF
SVLPKSMLNSTEHQWNPIIIETLLTQAGINSNNPLQITLSYDEQGKHTEIAQQMIRTLGQSDLIKVTPQALDWSQLLAVR
EQKNYQLSRAGWCAEYADPLPFLLHFHSKSVDNKSNYHNPQVDEKLERLQNQNLTEEVRTQLIQSVVKHLDDDVAVLPMF
QYYRRVALDPTILGIDLNNDSEVIYSKHLYRMKPKD

Specific function: Part of a binding-protein-dependent transport system [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=494, Percent_Identity=29.1497975708502, Blast_Score=192, Evalue=6e-50,
Organism=Escherichia coli, GI87081878, Length=541, Percent_Identity=27.3567467652495, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1789397, Length=526, Percent_Identity=26.0456273764259, Blast_Score=162, Evalue=6e-41,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58911; Mature: 58911

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSAKQAVKFFQNLVGLLACLLILSACERDLPVKHVEPAKPETLPIIDHNILKRAIYTNK
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCE
FQLDPHFVYSSAESAPLRDLFSGLLAYNEKGIAQPALAKTWFTENNKDWLFILDENAKWS
EECCCHHEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCC
NGKAVTAQDIVLSWQRLANPKNASPLAPYLVYMQLKHAKEILNEEKAVGELGVKALNATT
CCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
LQIRLEKPNLVLPKMLAHVALLPSYQGEIPNPNMLITNGDYALAAQTDKSLLLQAVKNNP
EEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHCCC
SFAKVEYHRISVLQSIKPFDIVENPLEGQQHNIWEFPRLCNYYYEFNFADPQLKHKEIRQ
CEEEEHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHEEECCCCCCHHHHHHHH
AIKTMILSARIPHQYGIANFSVLPKSMLNSTEHQWNPIIIETLLTQAGINSNNPLQITLS
HHHHHHHHHCCCHHHCCCCHHHCCHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEE
YDEQGKHTEIAQQMIRTLGQSDLIKVTPQALDWSQLLAVREQKNYQLSRAGWCAEYADPL
CCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHCCCCHHHCCCH
PFLLHFHSKSVDNKSNYHNPQVDEKLERLQNQNLTEEVRTQLIQSVVKHLDDDVAVLPMF
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEHHHH
QYYRRVALDPTILGIDLNNDSEVIYSKHLYRMKPKD
HHHHHHHCCCEEEEEEECCCCCEEEHHHHEECCCCC
>Mature Secondary Structure
MHSAKQAVKFFQNLVGLLACLLILSACERDLPVKHVEPAKPETLPIIDHNILKRAIYTNK
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCE
FQLDPHFVYSSAESAPLRDLFSGLLAYNEKGIAQPALAKTWFTENNKDWLFILDENAKWS
EECCCHHEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCC
NGKAVTAQDIVLSWQRLANPKNASPLAPYLVYMQLKHAKEILNEEKAVGELGVKALNATT
CCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
LQIRLEKPNLVLPKMLAHVALLPSYQGEIPNPNMLITNGDYALAAQTDKSLLLQAVKNNP
EEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHCCC
SFAKVEYHRISVLQSIKPFDIVENPLEGQQHNIWEFPRLCNYYYEFNFADPQLKHKEIRQ
CEEEEHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHEEECCCCCCHHHHHHHH
AIKTMILSARIPHQYGIANFSVLPKSMLNSTEHQWNPIIIETLLTQAGINSNNPLQITLS
HHHHHHHHHCCCHHHCCCCHHHCCHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEE
YDEQGKHTEIAQQMIRTLGQSDLIKVTPQALDWSQLLAVREQKNYQLSRAGWCAEYADPL
CCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHCCCCHHHCCCH
PFLLHFHSKSVDNKSNYHNPQVDEKLERLQNQNLTEEVRTQLIQSVVKHLDDDVAVLPMF
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEHHHH
QYYRRVALDPTILGIDLNNDSEVIYSKHLYRMKPKD
HHHHHHHCCCEEEEEEECCCCCEEEHHHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]