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The map label for this gene is smpB [H]

Identifier: 126208343

GI number: 126208343

Start: 1006549

End: 1007028

Strand: Direct

Name: smpB [H]

Synonym: APL_0867

Alternate gene names: 126208343

Gene position: 1006549-1007028 (Clockwise)

Preceding gene: 126208342

Following gene: 126208344

Centisome position: 44.25

GC content: 41.67

Gene sequence:

>480_bases
ATGGCAAAAAAACCAAAAGTAGCTTCGAATACGATTGCGTTGAATAAACGTGCTCGTCATGAATATTTTATCGAAGAAGA
AATTGAAGCCGGCTTAGAATTACAAGGCTGGGAAGTAAAATCGTTGCGTGCCGGTAAAGCGAATATCGGCGACAGTTACG
TGACATTTCGTAACGGCGAAGCGTTTCTATTCGGTGCAACCATCACACCATTAAATATGGCTTCTACTCACATTGTTTGT
GATCCGACTCGCACCCGTAAATTATTATTGAATAAACGTGAATTAGATTCGCTATTCGGTAAAGTAAATCGAGACGGTAT
GACGGTTGTGGCATTATCGCTCTACTGGAAAAACGCGTGGGCGAAAGTGAAAGTCGGCGTAGCAAAAGGTAAAAAACTAC
ACGATAAACGTGAGGACATTAAAGACCGTGAATGGCAAGTAGCGAAACAGCGTATTATGAAAAACGCAAATCGTGGCTAA

Upstream 100 bases:

>100_bases
GCAAATTTAACCGCTTGTCACTTGATTTTAAACGGTTACTGTTCACTGTTAGGCGGCTTTGCGGTAGAATCTTATAAATT
TTTCTCAATCTTTTTAAATT

Downstream 100 bases:

>100_bases
TTTCAAAAACTACGGATAACAGCAAGCACGGAACAAGCGGTCAAATTTGACCCGTTTTTTGCCTCTCCGTGTTGCCTGTG
CCGTCCGTGGTCACAATTAA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MAKKPKVASNTIALNKRARHEYFIEEEIEAGLELQGWEVKSLRAGKANIGDSYVTFRNGEAFLFGATITPLNMASTHIVC
DPTRTRKLLLNKRELDSLFGKVNRDGMTVVALSLYWKNAWAKVKVGVAKGKKLHDKREDIKDREWQVAKQRIMKNANRG

Sequences:

>Translated_159_residues
MAKKPKVASNTIALNKRARHEYFIEEEIEAGLELQGWEVKSLRAGKANIGDSYVTFRNGEAFLFGATITPLNMASTHIVC
DPTRTRKLLLNKRELDSLFGKVNRDGMTVVALSLYWKNAWAKVKVGVAKGKKLHDKREDIKDREWQVAKQRIMKNANRG
>Mature_158_residues
AKKPKVASNTIALNKRARHEYFIEEEIEAGLELQGWEVKSLRAGKANIGDSYVTFRNGEAFLFGATITPLNMASTHIVCD
PTRTRKLLLNKRELDSLFGKVNRDGMTVVALSLYWKNAWAKVKVGVAKGKKLHDKREDIKDREWQVAKQRIMKNANRG

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=156, Percent_Identity=81.4102564102564, Blast_Score=265, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18056; Mature: 17925

Theoretical pI: Translated: 10.70; Mature: 10.70

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKPKVASNTIALNKRARHEYFIEEEIEAGLELQGWEVKSLRAGKANIGDSYVTFRNGE
CCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCEECCCEEECCCCCCCCCCCCEEEEECCC
AFLFGATITPLNMASTHIVCDPTRTRKLLLNKRELDSLFGKVNRDGMTVVALSLYWKNAW
EEEEEEEECCCCCCCEEEEECCCHHHHHHHCHHHHHHHHHCCCCCCEEEEEEEEECCCCC
AKVKVGVAKGKKLHDKREDIKDREWQVAKQRIMKNANRG
EEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKKPKVASNTIALNKRARHEYFIEEEIEAGLELQGWEVKSLRAGKANIGDSYVTFRNGE
CCCCCCCCCEEEECCCCCHHHHHHHHHHCCCEECCCEEECCCCCCCCCCCCEEEEECCC
AFLFGATITPLNMASTHIVCDPTRTRKLLLNKRELDSLFGKVNRDGMTVVALSLYWKNAW
EEEEEEEECCCCCCCEEEEECCCHHHHHHHCHHHHHHHHHCCCCCCEEEEEEEEECCCCC
AKVKVGVAKGKKLHDKREDIKDREWQVAKQRIMKNANRG
EEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA