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The map label for this gene is sapA [H]

Identifier: 126208272

GI number: 126208272

Start: 917164

End: 918861

Strand: Reverse

Name: sapA [H]

Synonym: APL_0796

Alternate gene names: 126208272

Gene position: 918861-917164 (Counterclockwise)

Preceding gene: 126208273

Following gene: 126208271

Centisome position: 40.4

GC content: 40.99

Gene sequence:

>1698_bases
ATGGTTTCTTGTATGCGACAAGCGGTCGGTTTTATCCGCTTTTTTGCAATTTTAGCGATGATTCCGACCGCTTATAGTGC
GCCTCGTATTCCGCAAGCGTTATTGGATAACAGCTTGATTTATTGTACCAGCGTATCCGGTTTCAGCTTTAATCCGCAAA
AAGCGGACGTCGGCACCAATATGAATGTGGTGACGGAACAAATTTACGATAAGTTGTTCGAGTTTGACGCAGCGACAAAT
TCCTTAAAACCGAAACTGGTCGAAAGTTATCAGATTAGCGAAGACGGAAAAGTCATTACCTTAAAATTACGTCGTAAAGT
CGCCTTTCATGCTACGGAATGGTTTACGCCGACTCGTCCGTTTAATGCGGAAGACGTGGTTTTTTCATTGAACAGAATGA
TCGGGAATGTCGAAGAGCTTCCCGCTTTGGATTTTAATGAAGACGGTCAGAGAGAAGCATTCCATCAGAATCAGTATTAT
GCTTATCAATTTAAAGCGAATTTGGCGCATTATCCTTATTTTGAAAGCATCGCCTTAAAAAATAAAATCGAGCGTATTTC
GGCAGTAAACGACTATACGGTCAAGATTCATTTGGTTTCGCCCGATCAATCCGTTTTAGCGCATTTGGCAAGCCAATATG
CGGTTATTTTATCGAAAGAATACGCTTTACAATTAAATGCGGATGAAAACCTCGCACAACTAGATTTATTACCGGTCGGA
ACCGGTGTTTATCAACTGAGCAATTATGTACAGAATGAATATGTGCGTTTAAAACCGCATCCTAAATATTGGGGCAAAAA
AGCCAATATTGAAAATATGGTGGTGGATGTGTCCAGTAATGATACGGGGCGTATGGCAAAATATTTAAACGGTGAGTGTG
ATGTAGTCGCTTTGCCGGAGCCGAGCCAAAGAACGATCGTTCCTCATAATGAAATTATTGAAAGCCCGGGAGCTAATCTT
GCGTTTTTGGCGTTTAATATGCAAAAGCCGGCTATGAAAGAGATAGCGTTACGTCGCCGTATCGCCGGTGCAATTAATCG
CAAACGAATTGCCGACACGTTATTTTACGGTGCCGCCAATGTTGCGAATAACGTATTGCCAAACGTCTTATTCCCTTATT
CGAATCAACAACGTTATCAAGATCATACGACGCAAGAGCATAGTAAAAAAAGAGAAAAAACGGACCGCTTGAAATTATGG
GTATTGGATGAAAAGCGGGTTTATAATTTACATCCGTTAAAAATGGCGGAATTGATTCGGGCGGACTTGGAAAACGTGGG
GATTGATGTTGAGATTCGTACCGTCAGTCGTACGGATGTCGTGCAATTATCGGAAGACGGGCAGGCGGATTATGATTTGA
TTCTGACCGGATGGCTTGCCAATAATTTAGACCCGAATGCGTTTTTATCGCCTATTTTATCTTGCCGATCTCGTTATGAG
GTGACGAATTTAGCCAATTGGTGCAACCAAGAATTCGATCGTTGGTTGGAATTGGCGAGATTGAGTGAACAGCCCTATGT
TAAAATCGTGATTTATAAATTAATGCAAAGCCTATTAGAACGGGAATTACCGATTTTGCCGTTAGTAAATAGTAATCGAA
TTTTATTTGTAAATCCTAAAATCGAAAATGCGAAAGTCAGTCCTTTCGGGCAAGTGCATTTATCGGAACTAAAACTCAAA
CATAAAGAGAAGAAATAA

Upstream 100 bases:

>100_bases
ATACCCGAGTACGAGAAAATTGGCCCAACGTTTAGGCGTTTCTCATACGGCGGTTGCAAATAAGTTACGTGCATACGGCA
TTGGGAAATAAGGAAAGACT

Downstream 100 bases:

>100_bases
TGTTATTGGCATTTATTCGCAAATTATTTTTAACAGCGATCACGCTGATTTTGCTGACACTAATTAGCTATAACATCTTA
CTACGCGATCCTTTGAATCA

Product: peptide transport periplasmic protein SapA precursor

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MVSCMRQAVGFIRFFAILAMIPTAYSAPRIPQALLDNSLIYCTSVSGFSFNPQKADVGTNMNVVTEQIYDKLFEFDAATN
SLKPKLVESYQISEDGKVITLKLRRKVAFHATEWFTPTRPFNAEDVVFSLNRMIGNVEELPALDFNEDGQREAFHQNQYY
AYQFKANLAHYPYFESIALKNKIERISAVNDYTVKIHLVSPDQSVLAHLASQYAVILSKEYALQLNADENLAQLDLLPVG
TGVYQLSNYVQNEYVRLKPHPKYWGKKANIENMVVDVSSNDTGRMAKYLNGECDVVALPEPSQRTIVPHNEIIESPGANL
AFLAFNMQKPAMKEIALRRRIAGAINRKRIADTLFYGAANVANNVLPNVLFPYSNQQRYQDHTTQEHSKKREKTDRLKLW
VLDEKRVYNLHPLKMAELIRADLENVGIDVEIRTVSRTDVVQLSEDGQADYDLILTGWLANNLDPNAFLSPILSCRSRYE
VTNLANWCNQEFDRWLELARLSEQPYVKIVIYKLMQSLLERELPILPLVNSNRILFVNPKIENAKVSPFGQVHLSELKLK
HKEKK

Sequences:

>Translated_565_residues
MVSCMRQAVGFIRFFAILAMIPTAYSAPRIPQALLDNSLIYCTSVSGFSFNPQKADVGTNMNVVTEQIYDKLFEFDAATN
SLKPKLVESYQISEDGKVITLKLRRKVAFHATEWFTPTRPFNAEDVVFSLNRMIGNVEELPALDFNEDGQREAFHQNQYY
AYQFKANLAHYPYFESIALKNKIERISAVNDYTVKIHLVSPDQSVLAHLASQYAVILSKEYALQLNADENLAQLDLLPVG
TGVYQLSNYVQNEYVRLKPHPKYWGKKANIENMVVDVSSNDTGRMAKYLNGECDVVALPEPSQRTIVPHNEIIESPGANL
AFLAFNMQKPAMKEIALRRRIAGAINRKRIADTLFYGAANVANNVLPNVLFPYSNQQRYQDHTTQEHSKKREKTDRLKLW
VLDEKRVYNLHPLKMAELIRADLENVGIDVEIRTVSRTDVVQLSEDGQADYDLILTGWLANNLDPNAFLSPILSCRSRYE
VTNLANWCNQEFDRWLELARLSEQPYVKIVIYKLMQSLLERELPILPLVNSNRILFVNPKIENAKVSPFGQVHLSELKLK
HKEKK
>Mature_565_residues
MVSCMRQAVGFIRFFAILAMIPTAYSAPRIPQALLDNSLIYCTSVSGFSFNPQKADVGTNMNVVTEQIYDKLFEFDAATN
SLKPKLVESYQISEDGKVITLKLRRKVAFHATEWFTPTRPFNAEDVVFSLNRMIGNVEELPALDFNEDGQREAFHQNQYY
AYQFKANLAHYPYFESIALKNKIERISAVNDYTVKIHLVSPDQSVLAHLASQYAVILSKEYALQLNADENLAQLDLLPVG
TGVYQLSNYVQNEYVRLKPHPKYWGKKANIENMVVDVSSNDTGRMAKYLNGECDVVALPEPSQRTIVPHNEIIESPGANL
AFLAFNMQKPAMKEIALRRRIAGAINRKRIADTLFYGAANVANNVLPNVLFPYSNQQRYQDHTTQEHSKKREKTDRLKLW
VLDEKRVYNLHPLKMAELIRADLENVGIDVEIRTVSRTDVVQLSEDGQADYDLILTGWLANNLDPNAFLSPILSCRSRYE
VTNLANWCNQEFDRWLELARLSEQPYVKIVIYKLMQSLLERELPILPLVNSNRILFVNPKIENAKVSPFGQVHLSELKLK
HKEKK

Specific function: Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787551, Length=574, Percent_Identity=35.3658536585366, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI1789966, Length=533, Percent_Identity=30.3939962476548, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1787052, Length=482, Percent_Identity=24.2738589211618, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787762, Length=561, Percent_Identity=20.6773618538324, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789887, Length=441, Percent_Identity=23.3560090702948, Blast_Score=86, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 64596; Mature: 64596

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSCMRQAVGFIRFFAILAMIPTAYSAPRIPQALLDNSLIYCTSVSGFSFNPQKADVGTN
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCCCCCCC
MNVVTEQIYDKLFEFDAATNSLKPKLVESYQISEDGKVITLKLRRKVAFHATEWFTPTRP
HHHHHHHHHHHHHHHHCCCCCCCCHHHHCEEECCCCCEEEEEEECHHHHHHHHCCCCCCC
FNAEDVVFSLNRMIGNVEELPALDFNEDGQREAFHQNQYYAYQFKANLAHYPYFESIALK
CCHHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCHHHHHHH
NKIERISAVNDYTVKIHLVSPDQSVLAHLASQYAVILSKEYALQLNADENLAQLDLLPVG
HHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHEEEEEEEEEEEECCCCCCEEEEEEECC
TGVYQLSNYVQNEYVRLKPHPKYWGKKANIENMVVDVSSNDTGRMAKYLNGECDVVALPE
CHHHHHHHHHHCCEEEECCCCHHCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCEEECCC
PSQRTIVPHNEIIESPGANLAFLAFNMQKPAMKEIALRRRIAGAINRKRIADTLFYGAAN
CCCCEECCCHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VANNVLPNVLFPYSNQQRYQDHTTQEHSKKREKTDRLKLWVLDEKRVYNLHPLKMAELIR
HHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCHHHHHHHH
ADLENVGIDVEIRTVSRTDVVQLSEDGQADYDLILTGWLANNLDPNAFLSPILSCRSRYE
HHHHHCCCEEEEEEECCCCEEEECCCCCCCEEEEEEEHHHCCCCCHHHHHHHHHHHHHCC
VTNLANWCNQEFDRWLELARLSEQPYVKIVIYKLMQSLLERELPILPLVNSNRILFVNPK
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCC
IENAKVSPFGQVHLSELKLKHKEKK
CCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MVSCMRQAVGFIRFFAILAMIPTAYSAPRIPQALLDNSLIYCTSVSGFSFNPQKADVGTN
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCCCCCCC
MNVVTEQIYDKLFEFDAATNSLKPKLVESYQISEDGKVITLKLRRKVAFHATEWFTPTRP
HHHHHHHHHHHHHHHHCCCCCCCCHHHHCEEECCCCCEEEEEEECHHHHHHHHCCCCCCC
FNAEDVVFSLNRMIGNVEELPALDFNEDGQREAFHQNQYYAYQFKANLAHYPYFESIALK
CCHHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCHHHHHHH
NKIERISAVNDYTVKIHLVSPDQSVLAHLASQYAVILSKEYALQLNADENLAQLDLLPVG
HHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHEEEEEEEEEEEECCCCCCEEEEEEECC
TGVYQLSNYVQNEYVRLKPHPKYWGKKANIENMVVDVSSNDTGRMAKYLNGECDVVALPE
CHHHHHHHHHHCCEEEECCCCHHCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCEEECCC
PSQRTIVPHNEIIESPGANLAFLAFNMQKPAMKEIALRRRIAGAINRKRIADTLFYGAAN
CCCCEECCCHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VANNVLPNVLFPYSNQQRYQDHTTQEHSKKREKTDRLKLWVLDEKRVYNLHPLKMAELIR
HHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCHHHHHHHH
ADLENVGIDVEIRTVSRTDVVQLSEDGQADYDLILTGWLANNLDPNAFLSPILSCRSRYE
HHHHHCCCEEEEEEECCCCEEEECCCCCCCEEEEEEEHHHCCCCCHHHHHHHHHHHHHCC
VTNLANWCNQEFDRWLELARLSEQPYVKIVIYKLMQSLLERELPILPLVNSNRILFVNPK
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCC
IENAKVSPFGQVHLSELKLKHKEKK
CCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]