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The map label for this gene is ipk

Identifier: 126208254

GI number: 126208254

Start: 892484

End: 893341

Strand: Reverse

Name: ipk

Synonym: APL_0776

Alternate gene names: 126208254

Gene position: 893341-892484 (Counterclockwise)

Preceding gene: 126208255

Following gene: 126208253

Centisome position: 39.28

GC content: 44.06

Gene sequence:

>858_bases
ATGACGGAAAAAATAATTTTACCCAGTCCGGCCAAGTTAAATCTGTTTCTTTATATCACTAATAAACGTGCGGACGGTTA
TCACGAATTACAGACGCTGTTTCAGTTCTTAGATTTCGGCGATGATATTTCCCTTGAAGTGAATGAAAGCGGTGAAATCG
AGTTATTAAACGCAATAGAAGGGGTCGCGAAGGAACAAAACCTGATTTACCGTGCGGCAAAATTATTGCAAAATCATACC
GCTTGTAGCAAAGGGGCGAAAATCGGTGTGACGAAACGTTTGCCGATGGGCGGCGGTGTCGGCGGCGGTTCATCGAATGC
GGCGACCGTATTGGTCGGATTAAATCATTTCTGGCAAACCGGTTTAAGTTTGGAACAGCTTGCGGAATTAGGACTAAGTT
TGGGGGCGGACGTACCTATTTTTGTACGAGGTTTTGCCGCTTTTGCCGAAGGTGTCGGCGAAAAATTGGTGGCTTGCCAG
CCGCGAGAGTCGTGGTATGTGGTATTAAAACCGAACGTTTCCATTTCGACCGCTGCGGTTTTTCAAGATCCGAATTTGCC
GAGAAATACGCCGAAAAGAACATTAAGCCGACTTTTATCGGAAGAATGGACAAACGATTGCGAAAAAGTTGTGCGAGATC
ATTATTTCGAGGTTGAAGATCTTATTGCAGAATTGTTACAATATGCAACGTTTCGGCTGACAGGCACAGGAGCTTGTATT
TTCGCCGAATTTGAGAGTGAAGCGGAAGCAAAAGCCGTTTTTGCACATAAACCGCACAATATTTTCGGTTTTATTGCAAA
AGGACAAAATCGCTCTCCTTTACATCAAATGCTCAATTTAACCACTTCCCCACAATAG

Upstream 100 bases:

>100_bases
TGAAGATCGTCAGCCGAACTTACCGAAATTGATTGTTCTGGAAAACGGTTCTCAAACGTTAAAAATTCGTGTTGATCAAT
GGGCGTTTTAGGCGGAAAAC

Downstream 100 bases:

>100_bases
GATTCATTCCAATGCCAGATATTAAATTGTTTGCAGGTAATGCAACGCCTGAACTTGCAAAACGTATTGCAGAACGTCTT
TATACATCTCTCGGAGATGC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTEKIILPSPAKLNLFLYITNKRADGYHELQTLFQFLDFGDDISLEVNESGEIELLNAIEGVAKEQNLIYRAAKLLQNHT
ACSKGAKIGVTKRLPMGGGVGGGSSNAATVLVGLNHFWQTGLSLEQLAELGLSLGADVPIFVRGFAAFAEGVGEKLVACQ
PRESWYVVLKPNVSISTAAVFQDPNLPRNTPKRTLSRLLSEEWTNDCEKVVRDHYFEVEDLIAELLQYATFRLTGTGACI
FAEFESEAEAKAVFAHKPHNIFGFIAKGQNRSPLHQMLNLTTSPQ

Sequences:

>Translated_285_residues
MTEKIILPSPAKLNLFLYITNKRADGYHELQTLFQFLDFGDDISLEVNESGEIELLNAIEGVAKEQNLIYRAAKLLQNHT
ACSKGAKIGVTKRLPMGGGVGGGSSNAATVLVGLNHFWQTGLSLEQLAELGLSLGADVPIFVRGFAAFAEGVGEKLVACQ
PRESWYVVLKPNVSISTAAVFQDPNLPRNTPKRTLSRLLSEEWTNDCEKVVRDHYFEVEDLIAELLQYATFRLTGTGACI
FAEFESEAEAKAVFAHKPHNIFGFIAKGQNRSPLHQMLNLTTSPQ
>Mature_284_residues
TEKIILPSPAKLNLFLYITNKRADGYHELQTLFQFLDFGDDISLEVNESGEIELLNAIEGVAKEQNLIYRAAKLLQNHTA
CSKGAKIGVTKRLPMGGGVGGGSSNAATVLVGLNHFWQTGLSLEQLAELGLSLGADVPIFVRGFAAFAEGVGEKLVACQP
RESWYVVLKPNVSISTAAVFQDPNLPRNTPKRTLSRLLSEEWTNDCEKVVRDHYFEVEDLIAELLQYATFRLTGTGACIF
AEFESEAEAKAVFAHKPHNIFGFIAKGQNRSPLHQMLNLTTSPQ

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=277, Percent_Identity=61.0108303249097, Blast_Score=337, Evalue=7e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_ACTP2 (A3N0D8)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053479.1
- ProteinModelPortal:   A3N0D8
- SMR:   A3N0D8
- STRING:   A3N0D8
- GeneID:   4850291
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0776
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   LNLFLHV
- PhylomeDB:   A3N0D8
- ProtClustDB:   PRK00343
- BioCyc:   APLE416269:APL_0776-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31309; Mature: 31178

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKIILPSPAKLNLFLYITNKRADGYHELQTLFQFLDFGDDISLEVNESGEIELLNAIE
CCCCEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEHHHHHH
GVAKEQNLIYRAAKLLQNHTACSKGAKIGVTKRLPMGGGVGGGSSNAATVLVGLNHFWQT
HHHHHHHHHHHHHHHHHHHHHHHCCCEECCEEECCCCCCCCCCCCCCEEEEEEHHHHHHC
GLSLEQLAELGLSLGADVPIFVRGFAAFAEGVGEKLVACQPRESWYVVLKPNVSISTAAV
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHEEECCCCCEEEEECCCCCEEEEEE
FQDPNLPRNTPKRTLSRLLSEEWTNDCEKVVRDHYFEVEDLIAELLQYATFRLTGTGACI
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCEEE
FAEFESEAEAKAVFAHKPHNIFGFIAKGQNRSPLHQMLNLTTSPQ
EEECCCCCCHHEEEECCCCCEEEEEECCCCCCHHHHHHHCCCCCC
>Mature Secondary Structure 
TEKIILPSPAKLNLFLYITNKRADGYHELQTLFQFLDFGDDISLEVNESGEIELLNAIE
CCCEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEHHHHHH
GVAKEQNLIYRAAKLLQNHTACSKGAKIGVTKRLPMGGGVGGGSSNAATVLVGLNHFWQT
HHHHHHHHHHHHHHHHHHHHHHHCCCEECCEEECCCCCCCCCCCCCCEEEEEEHHHHHHC
GLSLEQLAELGLSLGADVPIFVRGFAAFAEGVGEKLVACQPRESWYVVLKPNVSISTAAV
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHEEECCCCCEEEEECCCCCEEEEEE
FQDPNLPRNTPKRTLSRLLSEEWTNDCEKVVRDHYFEVEDLIAELLQYATFRLTGTGACI
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCEEE
FAEFESEAEAKAVFAHKPHNIFGFIAKGQNRSPLHQMLNLTTSPQ
EEECCCCCCHHEEEECCCCCEEEEEECCCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA