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The map label for this gene is lolB

Identifier: 126208255

GI number: 126208255

Start: 893351

End: 893983

Strand: Reverse

Name: lolB

Synonym: APL_0777

Alternate gene names: 126208255

Gene position: 893983-893351 (Counterclockwise)

Preceding gene: 126208256

Following gene: 126208254

Centisome position: 39.3

GC content: 41.07

Gene sequence:

>633_bases
ATGAAAAAATTCACTAAAATTCTCTCTCTTTCAACCTTACTTTTCCTTGCCGGCTGCCAATCGGTATTAAACGAACCGAC
CGAAGTGCAACAACCCGGCGTACAAATTCCGCATAATGACGCGCAATGGCAACAACATCTGCAACAGTTGGCAAAAATCC
AAAGTTATTCGGCTAAAGGACAAATCGGTTATATTTCTCCGGAAGAACGTTTTTCTTCTCATTTCGATTGGCAGTATCGT
ACGCCGGCTAATTTCGGTTTGGAACTTTCCTCTAATTTATCCAGTAAATCATTAAAATTGCATCGTAATGTGCGAGGCTT
AACCATTTCGGACAGCGAAGGTAATTCTCGTTCCGATCGTGATATGGACAGTTTGATGAAAGAAATTATCGGCGTTGCTT
TCCCGATAGATCAATTCGCTTATTGGTTGAAAGGGCAGCCGGAACAAGACGGTAATTATATCGTAAACGACAAACGCCAA
CTTTCACAATTTAGCTATCACATCAATGGCGAAGTGTGGAAAGCGAGTTACGTACAATATCATGAAGATCGTCAGCCGAA
CTTACCGAAATTGATTGTTCTGGAAAACGGTTCTCAAACGTTAAAAATTCGTGTTGATCAATGGGCGTTTTAG

Upstream 100 bases:

>100_bases
CACCGATAAGGTGCCTTTTTCTTTGAAGACAAGCGGTCGGATTTCCGTTAAACTTTGCAAAGTTTTTAGACATTCAGACC
GCTTGTAAAGCAGGAAATCT

Downstream 100 bases:

>100_bases
GCGGAAAACATGACGGAAAAAATAATTTTACCCAGTCCGGCCAAGTTAAATCTGTTTCTTTATATCACTAATAAACGTGC
GGACGGTTATCACGAATTAC

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKKFTKILSLSTLLFLAGCQSVLNEPTEVQQPGVQIPHNDAQWQQHLQQLAKIQSYSAKGQIGYISPEERFSSHFDWQYR
TPANFGLELSSNLSSKSLKLHRNVRGLTISDSEGNSRSDRDMDSLMKEIIGVAFPIDQFAYWLKGQPEQDGNYIVNDKRQ
LSQFSYHINGEVWKASYVQYHEDRQPNLPKLIVLENGSQTLKIRVDQWAF

Sequences:

>Translated_210_residues
MKKFTKILSLSTLLFLAGCQSVLNEPTEVQQPGVQIPHNDAQWQQHLQQLAKIQSYSAKGQIGYISPEERFSSHFDWQYR
TPANFGLELSSNLSSKSLKLHRNVRGLTISDSEGNSRSDRDMDSLMKEIIGVAFPIDQFAYWLKGQPEQDGNYIVNDKRQ
LSQFSYHINGEVWKASYVQYHEDRQPNLPKLIVLENGSQTLKIRVDQWAF
>Mature_210_residues
MKKFTKILSLSTLLFLAGCQSVLNEPTEVQQPGVQIPHNDAQWQQHLQQLAKIQSYSAKGQIGYISPEERFSSHFDWQYR
TPANFGLELSSNLSSKSLKLHRNVRGLTISDSEGNSRSDRDMDSLMKEIIGVAFPIDQFAYWLKGQPEQDGNYIVNDKRQ
LSQFSYHINGEVWKASYVQYHEDRQPNLPKLIVLENGSQTLKIRVDQWAF

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family

Homologues:

Organism=Escherichia coli, GI1787460, Length=206, Percent_Identity=26.6990291262136, Blast_Score=101, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LOLB_ACTP2 (A3N0D9)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053480.1
- ProteinModelPortal:   A3N0D9
- SMR:   A3N0D9
- STRING:   A3N0D9
- GeneID:   4848899
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0777
- eggNOG:   COG3017
- HOGENOM:   HBG296876
- OMA:   WLQRQDY
- PhylomeDB:   A3N0D9
- ProtClustDB:   PRK00022
- BioCyc:   APLE416269:APL_0777-MONOMER
- HAMAP:   MF_00233
- InterPro:   IPR004565
- TIGRFAMs:   TIGR00548

Pfam domain/function: PF03550 LolB

EC number: NA

Molecular weight: Translated: 24162; Mature: 24162

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFTKILSLSTLLFLAGCQSVLNEPTEVQQPGVQIPHNDAQWQQHLQQLAKIQSYSAKG
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
QIGYISPEERFSSHFDWQYRTPANFGLELSSNLSSKSLKLHRNVRGLTISDSEGNSRSDR
CEEECCHHHHHHCCCCEEEECCCCCCEEECCCCCCHHHHHHCCCCCEEEECCCCCCCCCH
DMDSLMKEIIGVAFPIDQFAYWLKGQPEQDGNYIVNDKRQLSQFSYHINGEVWKASYVQY
HHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEECCHHHHHHHHEECCCEEEEHHHHHH
HEDRQPNLPKLIVLENGSQTLKIRVDQWAF
HCCCCCCCCEEEEEECCCCEEEEEECCCCC
>Mature Secondary Structure
MKKFTKILSLSTLLFLAGCQSVLNEPTEVQQPGVQIPHNDAQWQQHLQQLAKIQSYSAKG
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
QIGYISPEERFSSHFDWQYRTPANFGLELSSNLSSKSLKLHRNVRGLTISDSEGNSRSDR
CEEECCHHHHHHCCCCEEEECCCCCCEEECCCCCCHHHHHHCCCCCEEEECCCCCCCCCH
DMDSLMKEIIGVAFPIDQFAYWLKGQPEQDGNYIVNDKRQLSQFSYHINGEVWKASYVQY
HHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEECCHHHHHHHHEECCCEEEEHHHHHH
HEDRQPNLPKLIVLENGSQTLKIRVDQWAF
HCCCCCCCCEEEEEECCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA