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The map label for this gene is topA [H]

Identifier: 126208176

GI number: 126208176

Start: 795991

End: 798600

Strand: Reverse

Name: topA [H]

Synonym: APL_0696

Alternate gene names: 126208176

Gene position: 798600-795991 (Counterclockwise)

Preceding gene: 126208178

Following gene: 126208169

Centisome position: 35.11

GC content: 43.41

Gene sequence:

>2610_bases
ATGGGAAAATCATTGGTTATTGTTGAGTCACCGGCGAAAGCTAAGACGATCAATAAATATTTAGGTAGTGATTATGTGGT
GAAATCGAGTGTCGGTCATATCCGCGATTTACCTAAAAGCGGTAGTGAAAGTGAAAAAGGCGAAAAAGCGAAACCGATTT
CAACCAAAGGCATGACGGCGGAACAAAAAGCAAAACTAAAAGCAGAAAAAGATCGTGCCGCATTGGTAAAACGTATGGGG
ATCGACCCTTACCATGATTGGAAAGCGAATTATCAAATTCTTCCGGATAAAGAAAAGGTGGTTTCCGAATTAAAATCTTT
AGCCAAAAAAGCCGATCATATTTATCTCGCAACCGACTTGGATAGAGAAGGGGAAGCGATTGCTTGGCATTTGCGAGAGG
TTATCGGCGGTGACGACGAACGTTTTAGCCGTGTCGTATTTAACGAAATTACCAGAAATGCCATTCAAAAAGCGTTTGAA
CAACCGGATCATTTGAATTTAGACCGTGTAAACGCACAACAAACACGCCGCTTTTTAGACCGCGTGGTGGGTTTTATGGT
ATCGCCTTTACTTTGGAAAAAAGTCGCACGGGGCTTATCCGCAGGGCGTGTACAGTCGGTTGCAGTGAAACTTTTAGTCG
AGCGCGAGCGTGAAATTAAAGCGTTCCAACCGGAAGAGTATTGGGAAATTTTAGCCAATACGCTAACGGCTAAAGGAAAA
TTACCGCTTGATTTAACCGGCTATAAAGGTAAAAAATTCTCGGCAAAAAACCAAAAACAAGCGGATGAAGCGGTAAAATC
GTTACAAGGTAGCGAATTTATCGTTTCAGAGATTGATAAAAAGCCGACTTCCTCAAAAGCAAGCCCGCCGTTTATTACTT
CAACGCTACAACAAACGGCAAGTACTCGTTTAGGTTTTGGTGTGAAGAAAACCATGATGTTGGCGCAGCGCTTATATGAA
GCGGGTTATATCACCTATATGCGTACCGACTCAACCAATCTAAGCCAAGACGCATTAGCGATGGCACGCGGTTATATTGA
AAATCAATTCGGAGCCGAGTACCTACCGGCTAAACCGAATTTCTATTCAAGTAAAGAAGGTGCGCAAGAAGCGCATGAAG
CGATTCGTCCGTCAGACGTAAATGTCAAAATGGCTGATTTAAAAGGTATGGAAAAAGACGCAGAGCGTCTGTATGATCTT
ATTTGGCGTCGTTTCTTAGCTTGTCAGATGACACCGGCTCGTTATGATTCAACCAGCCTTACTGTTACGGCAGGCGATTA
TGAATTAAAGGCGAAGGGGCGTGTATTACGTTTTGACGGCTGGACGAAAGCATTACCGGTACAAGGTAAAACGGCTGAAG
ACCAAGTGTTACCGGAAGTCGCACTCAATGAAAAATTAGCGCTAGAGGAAGTATTACCTAGCCAACACTTTACTAAACCG
CCGGCGCGTTTCTCTGAAGCGGCATTAGTCAAAGAATTGGAAAAACGTGGTATCGGTCGGCCATCAACCTACGCTGCGAT
TATTTCGACTATTCAAGAGCGTGGTTACGTACGTACTGAAAATCGCCGTTTCTATGCGGAAAAAATGGGTGAAATCGTTA
CCGATCGTTTAGACGAATCCTTTAGCGATCTAATGAATTACGACTTTACCGCCAATATGGAAGAAACACTTGACCAAATT
GCGGAAGGTAATAAAAACTGGAAAGAGGCGCTTAATAATTTCTTCAAAGATTTCTCTGAGCAGCTGACTAAAGCCGAATT
AGATGAGTTGGAAGGTGGTATGAAGCCGAATAGCCTTGTGCCAACGGAAATTGACTGTCCGACTTGTGCAAGAAAAATGG
CAATTCGCACCGCTTCTACCGGTGTATTCTTAGGTTGTACCGGTTATGCGTTACCGCCGAAAGAGCGTTGTAAAACTACA
ATGAATCTGATTCCGGAATCGGAATTACTCAATGTGTTGGATGAAGAATCGGAAGCGAAAGCATTATTAGCCCGTAAACG
TTGTCCGAAATGTGATTCGGCAATGGATAGCTATGTGATCGATCCGCAACGTAAATTACATATTTGTGGTAATAATCCGA
ATTGTGACGGTTACGTATTAGAACAAGGTTCATTCAAAATTAAAGGCTATGACGGTCCGATCGTTGAATGTGATAAATGC
GGTGCGGATATGCACCTCAAACTTGGTCGTTTCGGTAAATACATGGGTTGTACCGCGTGCGATAACACCCGTAAGATTCT
GAAAAACGGTGAAGTGGCTCCGCCGCGCGAAGAGCCGACGCATTTCCCGGAACTTAAATGTGAGAAATCGGATGCGTATT
TCGTATTACGTGACGGTGCAAGCGGTGTGTTTATGTCAGCACATAATTTCCCAAAATCGCGTGAAACACGCGCGCCAAAA
GTTGCCGAATTAGCACAATACCGTGATCGTTTACCGGAAAAATTACAATATCTTGCCGATGCACCTCAGCAAGATCCGGA
AGGAAATGAAGCGATTATCCGCTTTAGCCGTAAAGAAAAACGTCAATATGTGACCTCTGAAAAAGACGGTAAGGCGACTA
AATGGATTGTCGATTATCTTGACGGTAAATGGCTAGAACGTAAAAAATAA

Upstream 100 bases:

>100_bases
TATTGTCGGTGCATTTTGGGTCAAGTAGTATTAAATATCAACTAAAAAGAAATAAAATCTAATATATTTCTTAAATTATT
TATATTTTGGGTAGTTATTC

Downstream 100 bases:

>100_bases
TGTATTCGTAAATGAAAAACCACCGCAATAAGCGGTGGTTTTTTTATAGCGATTTGCAACCAATTAGCGTGTGTATTGCA
AGTAATTAGCAAGTAGGCTA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 869; Mature: 868

Protein sequence:

>869_residues
MGKSLVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPKSGSESEKGEKAKPISTKGMTAEQKAKLKAEKDRAALVKRMG
IDPYHDWKANYQILPDKEKVVSELKSLAKKADHIYLATDLDREGEAIAWHLREVIGGDDERFSRVVFNEITRNAIQKAFE
QPDHLNLDRVNAQQTRRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQPEEYWEILANTLTAKGK
LPLDLTGYKGKKFSAKNQKQADEAVKSLQGSEFIVSEIDKKPTSSKASPPFITSTLQQTASTRLGFGVKKTMMLAQRLYE
AGYITYMRTDSTNLSQDALAMARGYIENQFGAEYLPAKPNFYSSKEGAQEAHEAIRPSDVNVKMADLKGMEKDAERLYDL
IWRRFLACQMTPARYDSTSLTVTAGDYELKAKGRVLRFDGWTKALPVQGKTAEDQVLPEVALNEKLALEEVLPSQHFTKP
PARFSEAALVKELEKRGIGRPSTYAAIISTIQERGYVRTENRRFYAEKMGEIVTDRLDESFSDLMNYDFTANMEETLDQI
AEGNKNWKEALNNFFKDFSEQLTKAELDELEGGMKPNSLVPTEIDCPTCARKMAIRTASTGVFLGCTGYALPPKERCKTT
MNLIPESELLNVLDEESEAKALLARKRCPKCDSAMDSYVIDPQRKLHICGNNPNCDGYVLEQGSFKIKGYDGPIVECDKC
GADMHLKLGRFGKYMGCTACDNTRKILKNGEVAPPREEPTHFPELKCEKSDAYFVLRDGASGVFMSAHNFPKSRETRAPK
VAELAQYRDRLPEKLQYLADAPQQDPEGNEAIIRFSRKEKRQYVTSEKDGKATKWIVDYLDGKWLERKK

Sequences:

>Translated_869_residues
MGKSLVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPKSGSESEKGEKAKPISTKGMTAEQKAKLKAEKDRAALVKRMG
IDPYHDWKANYQILPDKEKVVSELKSLAKKADHIYLATDLDREGEAIAWHLREVIGGDDERFSRVVFNEITRNAIQKAFE
QPDHLNLDRVNAQQTRRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQPEEYWEILANTLTAKGK
LPLDLTGYKGKKFSAKNQKQADEAVKSLQGSEFIVSEIDKKPTSSKASPPFITSTLQQTASTRLGFGVKKTMMLAQRLYE
AGYITYMRTDSTNLSQDALAMARGYIENQFGAEYLPAKPNFYSSKEGAQEAHEAIRPSDVNVKMADLKGMEKDAERLYDL
IWRRFLACQMTPARYDSTSLTVTAGDYELKAKGRVLRFDGWTKALPVQGKTAEDQVLPEVALNEKLALEEVLPSQHFTKP
PARFSEAALVKELEKRGIGRPSTYAAIISTIQERGYVRTENRRFYAEKMGEIVTDRLDESFSDLMNYDFTANMEETLDQI
AEGNKNWKEALNNFFKDFSEQLTKAELDELEGGMKPNSLVPTEIDCPTCARKMAIRTASTGVFLGCTGYALPPKERCKTT
MNLIPESELLNVLDEESEAKALLARKRCPKCDSAMDSYVIDPQRKLHICGNNPNCDGYVLEQGSFKIKGYDGPIVECDKC
GADMHLKLGRFGKYMGCTACDNTRKILKNGEVAPPREEPTHFPELKCEKSDAYFVLRDGASGVFMSAHNFPKSRETRAPK
VAELAQYRDRLPEKLQYLADAPQQDPEGNEAIIRFSRKEKRQYVTSEKDGKATKWIVDYLDGKWLERKK
>Mature_868_residues
GKSLVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPKSGSESEKGEKAKPISTKGMTAEQKAKLKAEKDRAALVKRMGI
DPYHDWKANYQILPDKEKVVSELKSLAKKADHIYLATDLDREGEAIAWHLREVIGGDDERFSRVVFNEITRNAIQKAFEQ
PDHLNLDRVNAQQTRRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQPEEYWEILANTLTAKGKL
PLDLTGYKGKKFSAKNQKQADEAVKSLQGSEFIVSEIDKKPTSSKASPPFITSTLQQTASTRLGFGVKKTMMLAQRLYEA
GYITYMRTDSTNLSQDALAMARGYIENQFGAEYLPAKPNFYSSKEGAQEAHEAIRPSDVNVKMADLKGMEKDAERLYDLI
WRRFLACQMTPARYDSTSLTVTAGDYELKAKGRVLRFDGWTKALPVQGKTAEDQVLPEVALNEKLALEEVLPSQHFTKPP
ARFSEAALVKELEKRGIGRPSTYAAIISTIQERGYVRTENRRFYAEKMGEIVTDRLDESFSDLMNYDFTANMEETLDQIA
EGNKNWKEALNNFFKDFSEQLTKAELDELEGGMKPNSLVPTEIDCPTCARKMAIRTASTGVFLGCTGYALPPKERCKTTM
NLIPESELLNVLDEESEAKALLARKRCPKCDSAMDSYVIDPQRKLHICGNNPNCDGYVLEQGSFKIKGYDGPIVECDKCG
ADMHLKLGRFGKYMGCTACDNTRKILKNGEVAPPREEPTHFPELKCEKSDAYFVLRDGASGVFMSAHNFPKSRETRAPKV
AELAQYRDRLPEKLQYLADAPQQDPEGNEAIIRFSRKEKRQYVTSEKDGKATKWIVDYLDGKWLERKK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=568, Percent_Identity=25.1760563380282, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI4507635, Length=523, Percent_Identity=25.6214149139579, Blast_Score=133, Evalue=7e-31,
Organism=Escherichia coli, GI1787529, Length=873, Percent_Identity=71.1340206185567, Blast_Score=1316, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=574, Percent_Identity=23.5191637630662, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32563869, Length=586, Percent_Identity=24.7440273037543, Blast_Score=138, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=504, Percent_Identity=24.4047619047619, Blast_Score=129, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6323263, Length=478, Percent_Identity=25.1046025104602, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24585251, Length=634, Percent_Identity=24.9211356466877, Blast_Score=134, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24640096, Length=490, Percent_Identity=24.2857142857143, Blast_Score=131, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 97908; Mature: 97776

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSLVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPKSGSESEKGEKAKPISTKGMTA
CCCCEEEEECCCHHHHHHHHHCCCEEEHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCH
EQKAKLKAEKDRAALVKRMGIDPYHDWKANYQILPDKEKVVSELKSLAKKADHIYLATDL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCEEEEEECC
DREGEAIAWHLREVIGGDDERFSRVVFNEITRNAIQKAFEQPDHLNLDRVNAQQTRRFLD
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHH
RVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQPEEYWEILANTLTAKGK
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
LPLDLTGYKGKKFSAKNQKQADEAVKSLQGSEFIVSEIDKKPTSSKASPPFITSTLQQTA
CCEEECCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
STRLGFGVKKTMMLAQRLYEAGYITYMRTDSTNLSQDALAMARGYIENQFGAEYLPAKPN
HHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
FYSSKEGAQEAHEAIRPSDVNVKMADLKGMEKDAERLYDLIWRRFLACQMTPARYDSTSL
CCCCCCHHHHHHHCCCCCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
TVTAGDYELKAKGRVLRFDGWTKALPVQGKTAEDQVLPEVALNEKLALEEVLPSQHFTKP
EEEECCEEEECCCEEEEECCCCCCCCCCCCCCHHHHCHHHHHCCHHHHHHHCCCCCCCCC
PARFSEAALVKELEKRGIGRPSTYAAIISTIQERGYVRTENRRFYAEKMGEIVTDRLDES
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHH
FSDLMNYDFTANMEETLDQIAEGNKNWKEALNNFFKDFSEQLTKAELDELEGGMKPNSLV
HHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PTEIDCPTCARKMAIRTASTGVFLGCTGYALPPKERCKTTMNLIPESELLNVLDEESEAK
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHH
ALLARKRCPKCDSAMDSYVIDPQRKLHICGNNPNCDGYVLEQGSFKIKGYDGPIVECDKC
HHHHHHCCCCCHHHHHHEEECCCCEEEEECCCCCCCEEEEECCCEEEECCCCCEEEHHHC
GADMHLKLGRFGKYMGCTACDNTRKILKNGEVAPPREEPTHFPELKCEKSDAYFVLRDGA
CCCCEEEHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCEEEEECCC
SGVFMSAHNFPKSRETRAPKVAELAQYRDRLPEKLQYLADAPQQDPEGNEAIIRFSRKEK
CEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEEHHHHH
RQYVTSEKDGKATKWIVDYLDGKWLERKK
HHHHCCCCCCCHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure 
GKSLVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPKSGSESEKGEKAKPISTKGMTA
CCCEEEEECCCHHHHHHHHHCCCEEEHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCH
EQKAKLKAEKDRAALVKRMGIDPYHDWKANYQILPDKEKVVSELKSLAKKADHIYLATDL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCEEEEEECC
DREGEAIAWHLREVIGGDDERFSRVVFNEITRNAIQKAFEQPDHLNLDRVNAQQTRRFLD
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHH
RVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQPEEYWEILANTLTAKGK
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
LPLDLTGYKGKKFSAKNQKQADEAVKSLQGSEFIVSEIDKKPTSSKASPPFITSTLQQTA
CCEEECCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
STRLGFGVKKTMMLAQRLYEAGYITYMRTDSTNLSQDALAMARGYIENQFGAEYLPAKPN
HHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
FYSSKEGAQEAHEAIRPSDVNVKMADLKGMEKDAERLYDLIWRRFLACQMTPARYDSTSL
CCCCCCHHHHHHHCCCCCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
TVTAGDYELKAKGRVLRFDGWTKALPVQGKTAEDQVLPEVALNEKLALEEVLPSQHFTKP
EEEECCEEEECCCEEEEECCCCCCCCCCCCCCHHHHCHHHHHCCHHHHHHHCCCCCCCCC
PARFSEAALVKELEKRGIGRPSTYAAIISTIQERGYVRTENRRFYAEKMGEIVTDRLDES
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHH
FSDLMNYDFTANMEETLDQIAEGNKNWKEALNNFFKDFSEQLTKAELDELEGGMKPNSLV
HHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PTEIDCPTCARKMAIRTASTGVFLGCTGYALPPKERCKTTMNLIPESELLNVLDEESEAK
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHH
ALLARKRCPKCDSAMDSYVIDPQRKLHICGNNPNCDGYVLEQGSFKIKGYDGPIVECDKC
HHHHHHCCCCCHHHHHHEEECCCCEEEEECCCCCCCEEEEECCCEEEECCCCCEEEHHHC
GADMHLKLGRFGKYMGCTACDNTRKILKNGEVAPPREEPTHFPELKCEKSDAYFVLRDGA
CCCCEEEHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCEEEEECCC
SGVFMSAHNFPKSRETRAPKVAELAQYRDRLPEKLQYLADAPQQDPEGNEAIIRFSRKEK
CEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEEHHHHH
RQYVTSEKDGKATKWIVDYLDGKWLERKK
HHHHCCCCCCCHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]