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The map label for this gene is metB [H]

Identifier: 126208163

GI number: 126208163

Start: 780978

End: 782114

Strand: Reverse

Name: metB [H]

Synonym: APL_0683

Alternate gene names: 126208163

Gene position: 782114-780978 (Counterclockwise)

Preceding gene: 126208164

Following gene: 126208162

Centisome position: 34.39

GC content: 43.62

Gene sequence:

>1137_bases
ATGACCAAATACAGTAACATTGAAACCACATTAGTACAGTTAGGCAACCGCACCGACCCTAGAACGGGAGCGGTAGCCAC
ACCGATTGTTTTATCGACCGCTTATGGTCGTGGCGGTTTGGGCGAATCGACCGGTTGGGATTATATTCGTACCAAAAACC
CGACCCGTGCCGTGTTAGAGCAAGGTATTGCTGATTTAGAGGGGGGAGATGCCGGTTTTGCAATGGCATCGGGTATGGCG
GCAATTCAGTTAGTGATGTCATTGTTTAAAGCGCCTGATGAGTGGATTATTTCAAGTGATGTATATGGCGGCTCTTACCG
TTTATTCGATTTCAGTCATAAACACCACAATACAGTAAAACCGGTTTATGTAAATACCGCAGATTTGGCGGCGATTGAAG
CGGCAATTACCCCAAACACTAAAGCGATTTTTGTCGAAACACCGTCAAACCCATTAATGGAAGAGTGTGATGTTGATGCA
ATTTCTAAAATTGCGAAAAAACATAACTTAATGTTAATTGTGGATAATACCTTCTTAACGCCGATTTTATTCCGTCCGAT
TGAACACGGTGCGGATATCGTGATTCACAGTGCAACGAAATACCTTTCCGGTCATAATGATGTGTTAGCCGGTTTAATCG
TTGCGAAAGATTCGGAAGCAACCAAAAACGAAGCGGGTCAAAAGCTTTCGGAACGTTTATTCTACTTCCAAAACTGTGCT
GGTGCGGTGCTTTCGCCGTTTGATTCTTACCTTGCGGTGCGTGGCTTAAAAACCCTTGCGTTACGTATGGAACGCCACCA
ATCGAATGCTACGGAATTAGCGAGATTCTTAAGCGAGCAACCGGAGATTGAGTGCGTACTTTATTCAGGTAAGAGCGGTA
TGCTTTCATTCCGTTTACAAAAAGAAGAGTGGGTACCGAAATTCTTAAAAGCGATTAAGTTGATTACCTTTGCGGAAAGT
TTAGGCGGTACGGAAAGTTTTATCACTTATCCGTCCACTCAAACACATATGGATATTCCGGAAGCGGAGCGTATTGCACG
TGGTATCACCAATAACTTACTGCGTTTCTCAGTTGGCTTGGAACATGTTGAAGATTTAAAAGTGGATTTACGCCAAGCGT
TCGGACAACTAAAATAA

Upstream 100 bases:

>100_bases
TAGAAAGGATACACCTAGCGTGTCCCTATTGGTAAAATTGCAAGAATTGTGTATAGGGGCGCATTGAGTGTGTCCAAAAT
AAACTCAAAAAGGAATGAAA

Downstream 100 bases:

>100_bases
GTAAATTGGCATAACATTTTGGTGTTATGCCAAATTTATTTTGATTTTAGTATTAATTGTCATTATCATTACCCTCTATT
TTTATTAAGAGAGCTTAAAC

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTKYSNIETTLVQLGNRTDPRTGAVATPIVLSTAYGRGGLGESTGWDYIRTKNPTRAVLEQGIADLEGGDAGFAMASGMA
AIQLVMSLFKAPDEWIISSDVYGGSYRLFDFSHKHHNTVKPVYVNTADLAAIEAAITPNTKAIFVETPSNPLMEECDVDA
ISKIAKKHNLMLIVDNTFLTPILFRPIEHGADIVIHSATKYLSGHNDVLAGLIVAKDSEATKNEAGQKLSERLFYFQNCA
GAVLSPFDSYLAVRGLKTLALRMERHQSNATELARFLSEQPEIECVLYSGKSGMLSFRLQKEEWVPKFLKAIKLITFAES
LGGTESFITYPSTQTHMDIPEAERIARGITNNLLRFSVGLEHVEDLKVDLRQAFGQLK

Sequences:

>Translated_378_residues
MTKYSNIETTLVQLGNRTDPRTGAVATPIVLSTAYGRGGLGESTGWDYIRTKNPTRAVLEQGIADLEGGDAGFAMASGMA
AIQLVMSLFKAPDEWIISSDVYGGSYRLFDFSHKHHNTVKPVYVNTADLAAIEAAITPNTKAIFVETPSNPLMEECDVDA
ISKIAKKHNLMLIVDNTFLTPILFRPIEHGADIVIHSATKYLSGHNDVLAGLIVAKDSEATKNEAGQKLSERLFYFQNCA
GAVLSPFDSYLAVRGLKTLALRMERHQSNATELARFLSEQPEIECVLYSGKSGMLSFRLQKEEWVPKFLKAIKLITFAES
LGGTESFITYPSTQTHMDIPEAERIARGITNNLLRFSVGLEHVEDLKVDLRQAFGQLK
>Mature_377_residues
TKYSNIETTLVQLGNRTDPRTGAVATPIVLSTAYGRGGLGESTGWDYIRTKNPTRAVLEQGIADLEGGDAGFAMASGMAA
IQLVMSLFKAPDEWIISSDVYGGSYRLFDFSHKHHNTVKPVYVNTADLAAIEAAITPNTKAIFVETPSNPLMEECDVDAI
SKIAKKHNLMLIVDNTFLTPILFRPIEHGADIVIHSATKYLSGHNDVLAGLIVAKDSEATKNEAGQKLSERLFYFQNCAG
AVLSPFDSYLAVRGLKTLALRMERHQSNATELARFLSEQPEIECVLYSGKSGMLSFRLQKEEWVPKFLKAIKLITFAESL
GGTESFITYPSTQTHMDIPEAERIARGITNNLLRFSVGLEHVEDLKVDLRQAFGQLK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=378, Percent_Identity=38.8888888888889, Blast_Score=258, Evalue=7e-69,
Organism=Homo sapiens, GI299473759, Length=378, Percent_Identity=35.1851851851852, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI25453487, Length=377, Percent_Identity=34.7480106100796, Blast_Score=200, Evalue=1e-51,
Organism=Escherichia coli, GI1790375, Length=384, Percent_Identity=39.5833333333333, Blast_Score=264, Evalue=7e-72,
Organism=Escherichia coli, GI1789383, Length=355, Percent_Identity=27.887323943662, Blast_Score=127, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17538055, Length=381, Percent_Identity=39.3700787401575, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17560056, Length=381, Percent_Identity=39.1076115485564, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI71980708, Length=407, Percent_Identity=32.1867321867322, Blast_Score=186, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6319307, Length=393, Percent_Identity=36.8956743002545, Blast_Score=238, Evalue=8e-64,
Organism=Saccharomyces cerevisiae, GI6321254, Length=410, Percent_Identity=30.2439024390244, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=31.3364055299539, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6321170, Length=341, Percent_Identity=30.2052785923754, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321904, Length=409, Percent_Identity=29.3398533007335, Blast_Score=115, Evalue=9e-27,
Organism=Drosophila melanogaster, GI19922554, Length=390, Percent_Identity=39.4871794871795, Blast_Score=263, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41605; Mature: 41474

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYSNIETTLVQLGNRTDPRTGAVATPIVLSTAYGRGGLGESTGWDYIRTKNPTRAVLE
CCCCCCHHHHHHHHCCCCCCCCCCEECCHHEEECCCCCCCCCCCCCCCEECCCHHHHHHH
QGIADLEGGDAGFAMASGMAAIQLVMSLFKAPDEWIISSDVYGGSYRLFDFSHKHHNTVK
HCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEECCCCCCCCC
PVYVNTADLAAIEAAITPNTKAIFVETPSNPLMEECDVDAISKIAKKHNLMLIVDNTFLT
EEEECCHHHHHEEEEECCCCCEEEEECCCCCCHHHCCHHHHHHHHHHCCEEEEECCCHHH
PILFRPIEHGADIVIHSATKYLSGHNDVLAGLIVAKDSEATKNEAGQKLSERLFYFQNCA
HHHHHHHCCCCCEEEECHHHHHCCCCHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHC
GAVLSPFDSYLAVRGLKTLALRMERHQSNATELARFLSEQPEIECVLYSGKSGMLSFRLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEECCCCEEEEEEC
KEEWVPKFLKAIKLITFAESLGGTESFITYPSTQTHMDIPEAERIARGITNNLLRFSVGL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EHVEDLKVDLRQAFGQLK
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKYSNIETTLVQLGNRTDPRTGAVATPIVLSTAYGRGGLGESTGWDYIRTKNPTRAVLE
CCCCCHHHHHHHHCCCCCCCCCCEECCHHEEECCCCCCCCCCCCCCCEECCCHHHHHHH
QGIADLEGGDAGFAMASGMAAIQLVMSLFKAPDEWIISSDVYGGSYRLFDFSHKHHNTVK
HCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEECCCCCCCCC
PVYVNTADLAAIEAAITPNTKAIFVETPSNPLMEECDVDAISKIAKKHNLMLIVDNTFLT
EEEECCHHHHHEEEEECCCCCEEEEECCCCCCHHHCCHHHHHHHHHHCCEEEEECCCHHH
PILFRPIEHGADIVIHSATKYLSGHNDVLAGLIVAKDSEATKNEAGQKLSERLFYFQNCA
HHHHHHHCCCCCEEEECHHHHHCCCCHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHC
GAVLSPFDSYLAVRGLKTLALRMERHQSNATELARFLSEQPEIECVLYSGKSGMLSFRLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEECCCCEEEEEEC
KEEWVPKFLKAIKLITFAESLGGTESFITYPSTQTHMDIPEAERIARGITNNLLRFSVGL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EHVEDLKVDLRQAFGQLK
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]