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The map label for this gene is rrmJ

Identifier: 126208076

GI number: 126208076

Start: 678772

End: 679398

Strand: Direct

Name: rrmJ

Synonym: APL_0594

Alternate gene names: 126208076

Gene position: 678772-679398 (Clockwise)

Preceding gene: 126208073

Following gene: 126208077

Centisome position: 29.84

GC content: 45.93

Gene sequence:

>627_bases
ATGGGCAGAAAAAGAAGTGCCAGTTCCTCTCGCTGGTTGGCGGAGCATTTTAAAGACCAGTTCGTACAGAAAGCACACAA
ACAAAAGTTGCGTTCGCGCGCTTATTTTAAATTGGATGAAATTCAACAAACCGACCGTTTGTTCAAACCGGGCATGACCG
TAGTGGATTTAGGTGCGGCACCGGGCGGTTGGTCACAATATGCGGTAACGCAAATCGGTAGTAAAGGTCGAATTATCGCT
TGCGATATTTTAGATATGAACCCGATTGTCGGGGTGGACTTTCTACAAGGTGACTTCCGAGAAGAATCGGTGTTGAATGC
GCTATTAGAGCGAGTCGGCGACGATATGGTGGATGTGGTAATGTCGGATATGGCGCCGAATTTCAGCGGTATGCCGTCGG
TAGATATTCCTCGTGCAATGTATTTAGTGGAGTTGGCTTTAGATATGTGCCGTCAGGTACTTGCACCTAAAGGCAGTTTT
GTCGTCAAAGTGTTCCAAGGTGAAGGCTTTGACGATTATTTAAGAGATATTCGCGCAATGTTTACGACGGTGAAAGTACG
TAAACCGGAGGCTTCTCGAGATCGTTCAAGAGAAGTTTATATTGTCGCAACGGGATATAAAGGTTGA

Upstream 100 bases:

>100_bases
ATTTAACTTTCCGATAGCTTTTCTCTCTTGAATTGAGTTAAATTGCCCTTATTATGGTAAACTATATTTTTTAGGTAGAT
AGAGAAGAGATTATTAGAGA

Downstream 100 bases:

>100_bases
GATAAGCGGTCAAGTTTTGCAGAAATTTTGTAAAATCCGACCGCTTGTTAAAATGAAGATAACGAATTTTTTATTCTAAA
AGAGGAAATTAACCTTGAAC

Product: 23S rRNA methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MGRKRSASSSRWLAEHFKDQFVQKAHKQKLRSRAYFKLDEIQQTDRLFKPGMTVVDLGAAPGGWSQYAVTQIGSKGRIIA
CDILDMNPIVGVDFLQGDFREESVLNALLERVGDDMVDVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCRQVLAPKGSF
VVKVFQGEGFDDYLRDIRAMFTTVKVRKPEASRDRSREVYIVATGYKG

Sequences:

>Translated_208_residues
MGRKRSASSSRWLAEHFKDQFVQKAHKQKLRSRAYFKLDEIQQTDRLFKPGMTVVDLGAAPGGWSQYAVTQIGSKGRIIA
CDILDMNPIVGVDFLQGDFREESVLNALLERVGDDMVDVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCRQVLAPKGSF
VVKVFQGEGFDDYLRDIRAMFTTVKVRKPEASRDRSREVYIVATGYKG
>Mature_207_residues
GRKRSASSSRWLAEHFKDQFVQKAHKQKLRSRAYFKLDEIQQTDRLFKPGMTVVDLGAAPGGWSQYAVTQIGSKGRIIAC
DILDMNPIVGVDFLQGDFREESVLNALLERVGDDMVDVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCRQVLAPKGSFV
VKVFQGEGFDDYLRDIRAMFTTVKVRKPEASRDRSREVYIVATGYKG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=215, Percent_Identity=34.4186046511628, Blast_Score=138, Evalue=3e-33,
Organism=Homo sapiens, GI29029591, Length=191, Percent_Identity=32.9842931937173, Blast_Score=108, Evalue=2e-24,
Organism=Homo sapiens, GI29029589, Length=191, Percent_Identity=32.9842931937173, Blast_Score=108, Evalue=2e-24,
Organism=Homo sapiens, GI7110661, Length=191, Percent_Identity=32.9842931937173, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI194097365, Length=206, Percent_Identity=32.0388349514563, Blast_Score=105, Evalue=4e-23,
Organism=Escherichia coli, GI1789569, Length=206, Percent_Identity=81.0679611650485, Blast_Score=349, Evalue=7e-98,
Organism=Caenorhabditis elegans, GI17553474, Length=217, Percent_Identity=32.7188940092166, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17554650, Length=192, Percent_Identity=30.2083333333333, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71987550, Length=207, Percent_Identity=30.9178743961353, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71987561, Length=207, Percent_Identity=30.9178743961353, Blast_Score=91, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17553860, Length=207, Percent_Identity=30.9178743961353, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6319535, Length=200, Percent_Identity=32.5, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319796, Length=209, Percent_Identity=28.7081339712919, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321302, Length=99, Percent_Identity=41.4141414141414, Blast_Score=75, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21356387, Length=213, Percent_Identity=34.2723004694836, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI18859957, Length=206, Percent_Identity=33.0097087378641, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648639, Length=194, Percent_Identity=34.5360824742268, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24647580, Length=199, Percent_Identity=29.1457286432161, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_ACTP2 (A3MZW0)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053301.1
- ProteinModelPortal:   A3MZW0
- SMR:   A3MZW0
- STRING:   A3MZW0
- GeneID:   4849193
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0594
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   A3MZW0
- ProtClustDB:   PRK11188
- BioCyc:   APLE416269:APL_0594-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR004512
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461
- TIGRFAMs:   TIGR00438

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 23410; Mature: 23279

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: ACT_SITE 163-163 BINDING 62-62 BINDING 64-64 BINDING 82-82 BINDING 98-98 BINDING 123-123

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRKRSASSSRWLAEHFKDQFVQKAHKQKLRSRAYFKLDEIQQTDRLFKPGMTVVDLGAA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC
PGGWSQYAVTQIGSKGRIIACDILDMNPIVGVDFLQGDFREESVLNALLERVGDDMVDVV
CCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHHHCHHHHHHH
MSDMAPNFSGMPSVDIPRAMYLVELALDMCRQVLAPKGSFVVKVFQGEGFDDYLRDIRAM
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHH
FTTVKVRKPEASRDRSREVYIVATGYKG
HHHHHEECCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure 
GRKRSASSSRWLAEHFKDQFVQKAHKQKLRSRAYFKLDEIQQTDRLFKPGMTVVDLGAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC
PGGWSQYAVTQIGSKGRIIACDILDMNPIVGVDFLQGDFREESVLNALLERVGDDMVDVV
CCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHHHCHHHHHHH
MSDMAPNFSGMPSVDIPRAMYLVELALDMCRQVLAPKGSFVVKVFQGEGFDDYLRDIRAM
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHH
FTTVKVRKPEASRDRSREVYIVATGYKG
HHHHHEECCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA