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The map label for this gene is potE [H]

Identifier: 126176271

GI number: 126176271

Start: 4792763

End: 4794082

Strand: Direct

Name: potE [H]

Synonym: Sbal_4085

Alternate gene names: 126176271

Gene position: 4792763-4794082 (Clockwise)

Preceding gene: 126176270

Following gene: 126176272

Centisome position: 93.47

GC content: 48.79

Gene sequence:

>1320_bases
ATGAGTAAATCAGAAAATAAAATTGGCGTCGTCCAATTAACTATTATCACCATAGTCAACATGATGGGTTCGGGCATTAT
TATGCTGCCAACCCAATTAGCTCAAGTCGGTACTATTTCGATTTTATCTTGGTTAGTCACAGCGGCGGGTTCAACTGCTC
TCGCCTACGCCTTTGCCAAGTGCGGTATGTTCAGTAAGAAATCCGGTGGTATGGGCGGTTATGCCGAATATGCCTTCGGC
CGATCTGGCAACTTTATGGCTAACTACACCTATGCCGTTTCGCTGCTCATCGCCAACGTCGCCATTGCGATCTCGGCCGT
GGGTTATGCCGCCGTGCTGCTTGATGTAAACCTAAGCCCAATGGCCATTTGTCTTGCAACCATTGGCGTACTTTGGCTGG
CAACGATTGCTAACTTCGGTGGTGCCCGTATCACAGGTCGCGTAAGTAGCGTGACTGTGTGGGGGATTATTCTGCCCGTG
ATTGGCGTGTCGTTAATTGGTTGGTTCTGGTTCGACATCGACCTCTACAAAGGCGCATGGAACCCGCATGATATGCCATT
CTTCAAAGCGTTAGGCGGTTCTATCGCGATGACTTTGTGGGCATTCCTTGGTTTAGAATCGGCCTGTGCCAACTCCGAAG
CCGTCGATAATCCAGAGAAAAACGTCCCTATCGCAGTAATGGGCGGTACTTTAGGTGCAGCAGTAATTTATATCGTCTCT
ACTAACGTCATTGCCGGTATTGTGCCAAACGCCGAACTTGCTATGTCGAACGCGCCCTTCGGTTTAGCCTTCGCGCAAAT
GTTTAACCCTGTGGTCGGTAAAATCGTGATGGCCTGTGCCATTATCTCTTGTACTGGCTCGCTGCTAGGTTGGCAGTTCA
CTATTGCCCAAGTGTTTAAAGCCTCTGCCGATGAAGGATTCTTCCCTAAAATCTTCTCTAAAGTCAGTAAAGCCGATGCG
CCAATTTGGGGTATGACCATTATCGTTTGTATTCAAACCGTTTTATCTCTGATGACTATTAGCCCATCACTGAGCAAACA
GTTCGAAGCACTCGTTAACCTCGCCGTCGTCACCAACATTGTGCCTTACATACTATCGATGGCCGCCCTTGGCATAATGC
AAAGACAGCAAAAAGTACCGGTTAATAAAGCCCGTATTGCCAACGTGATTGCCGTGATCGGTGCCTTATATAGCTTCTAT
GCCCTGTATAGCTCAGGCGAAACCGCAGTAATGCTTGGCGCCATGGCCACCTTCTTCGGTTGGACTATCTTCGGCGTGAT
CTCAAACAAAGCACCCGTTACCGATACGCGTCCAGCCTAA

Upstream 100 bases:

>100_bases
TACTTAATAAATAATGAAGACTGACTGCAGAGTTTATTAACCCCGCTCTGCAGTCAATTTCATACTCCCTATAATAAGTC
ACCTACATTAGGTTATTAGT

Downstream 100 bases:

>100_bases
ATCCGGCTAGCGCACTATCGAAAACGATTAATCACACCATCTAAAAGAACGGTTACTCAGGAATCATAGGTTAAGCCTAT
GATCCCCTGGAGAATACTTG

Product: putrescine transporter

Products: Proton [Cytoplasm]; putrescine [Cytoplasm] [C]

Alternate protein names: Putrescine transport protein [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSKSENKIGVVQLTIITIVNMMGSGIIMLPTQLAQVGTISILSWLVTAAGSTALAYAFAKCGMFSKKSGGMGGYAEYAFG
RSGNFMANYTYAVSLLIANVAIAISAVGYAAVLLDVNLSPMAICLATIGVLWLATIANFGGARITGRVSSVTVWGIILPV
IGVSLIGWFWFDIDLYKGAWNPHDMPFFKALGGSIAMTLWAFLGLESACANSEAVDNPEKNVPIAVMGGTLGAAVIYIVS
TNVIAGIVPNAELAMSNAPFGLAFAQMFNPVVGKIVMACAIISCTGSLLGWQFTIAQVFKASADEGFFPKIFSKVSKADA
PIWGMTIIVCIQTVLSLMTISPSLSKQFEALVNLAVVTNIVPYILSMAALGIMQRQQKVPVNKARIANVIAVIGALYSFY
ALYSSGETAVMLGAMATFFGWTIFGVISNKAPVTDTRPA

Sequences:

>Translated_439_residues
MSKSENKIGVVQLTIITIVNMMGSGIIMLPTQLAQVGTISILSWLVTAAGSTALAYAFAKCGMFSKKSGGMGGYAEYAFG
RSGNFMANYTYAVSLLIANVAIAISAVGYAAVLLDVNLSPMAICLATIGVLWLATIANFGGARITGRVSSVTVWGIILPV
IGVSLIGWFWFDIDLYKGAWNPHDMPFFKALGGSIAMTLWAFLGLESACANSEAVDNPEKNVPIAVMGGTLGAAVIYIVS
TNVIAGIVPNAELAMSNAPFGLAFAQMFNPVVGKIVMACAIISCTGSLLGWQFTIAQVFKASADEGFFPKIFSKVSKADA
PIWGMTIIVCIQTVLSLMTISPSLSKQFEALVNLAVVTNIVPYILSMAALGIMQRQQKVPVNKARIANVIAVIGALYSFY
ALYSSGETAVMLGAMATFFGWTIFGVISNKAPVTDTRPA
>Mature_438_residues
SKSENKIGVVQLTIITIVNMMGSGIIMLPTQLAQVGTISILSWLVTAAGSTALAYAFAKCGMFSKKSGGMGGYAEYAFGR
SGNFMANYTYAVSLLIANVAIAISAVGYAAVLLDVNLSPMAICLATIGVLWLATIANFGGARITGRVSSVTVWGIILPVI
GVSLIGWFWFDIDLYKGAWNPHDMPFFKALGGSIAMTLWAFLGLESACANSEAVDNPEKNVPIAVMGGTLGAAVIYIVST
NVIAGIVPNAELAMSNAPFGLAFAQMFNPVVGKIVMACAIISCTGSLLGWQFTIAQVFKASADEGFFPKIFSKVSKADAP
IWGMTIIVCIQTVLSLMTISPSLSKQFEALVNLAVVTNIVPYILSMAALGIMQRQQKVPVNKARIANVIAVIGALYSFYA
LYSSGETAVMLGAMATFFGWTIFGVISNKAPVTDTRPA

Specific function: Probable putrescine-ornithine antiporter [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786908, Length=438, Percent_Identity=72.3744292237443, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI2367353, Length=409, Percent_Identity=36.1858190709046, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1790575, Length=408, Percent_Identity=31.8627450980392, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1787890, Length=424, Percent_Identity=25.7075471698113, Blast_Score=105, Evalue=8e-24,
Organism=Drosophila melanogaster, GI45550968, Length=375, Percent_Identity=26.4, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19921172, Length=375, Percent_Identity=26.4, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004754 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 46226; Mature: 46095

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSENKIGVVQLTIITIVNMMGSGIIMLPTQLAQVGTISILSWLVTAAGSTALAYAFAK
CCCCCCCCEEEEHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
CGMFSKKSGGMGGYAEYAFGRSGNFMANYTYAVSLLIANVAIAISAVGYAAVLLDVNLSP
CCCCCCCCCCCCCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCH
MAICLATIGVLWLATIANFGGARITGRVSSVTVWGIILPVIGVSLIGWFWFDIDLYKGAW
HHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
NPHDMPFFKALGGSIAMTLWAFLGLESACANSEAVDNPEKNVPIAVMGGTLGAAVIYIVS
CCCCCHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHEE
TNVIAGIVPNAELAMSNAPFGLAFAQMFNPVVGKIVMACAIISCTGSLLGWQFTIAQVFK
HHHHEEECCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASADEGFFPKIFSKVSKADAPIWGMTIIVCIQTVLSLMTISPSLSKQFEALVNLAVVTNI
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VPYILSMAALGIMQRQQKVPVNKARIANVIAVIGALYSFYALYSSGETAVMLGAMATFFG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
WTIFGVISNKAPVTDTRPA
HHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SKSENKIGVVQLTIITIVNMMGSGIIMLPTQLAQVGTISILSWLVTAAGSTALAYAFAK
CCCCCCCEEEEHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
CGMFSKKSGGMGGYAEYAFGRSGNFMANYTYAVSLLIANVAIAISAVGYAAVLLDVNLSP
CCCCCCCCCCCCCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCH
MAICLATIGVLWLATIANFGGARITGRVSSVTVWGIILPVIGVSLIGWFWFDIDLYKGAW
HHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
NPHDMPFFKALGGSIAMTLWAFLGLESACANSEAVDNPEKNVPIAVMGGTLGAAVIYIVS
CCCCCHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHEE
TNVIAGIVPNAELAMSNAPFGLAFAQMFNPVVGKIVMACAIISCTGSLLGWQFTIAQVFK
HHHHEEECCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASADEGFFPKIFSKVSKADAPIWGMTIIVCIQTVLSLMTISPSLSKQFEALVNLAVVTNI
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VPYILSMAALGIMQRQQKVPVNKARIANVIAVIGALYSFYALYSSGETAVMLGAMATFFG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
WTIFGVISNKAPVTDTRPA
HHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; putrescine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + putrescine [Periplasm] = Proton [Cytoplasm] + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]