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The map label for this gene is priA [H]

Identifier: 126176035

GI number: 126176035

Start: 4517181

End: 4519376

Strand: Direct

Name: priA [H]

Synonym: Sbal_3844

Alternate gene names: 126176035

Gene position: 4517181-4519376 (Clockwise)

Preceding gene: 126176001

Following gene: 126176036

Centisome position: 88.1

GC content: 50.59

Gene sequence:

>2196_bases
ATGTCATTGTTTGTCGAAGTTGCGTTACCTGTGCCTATGCGGCAAACCTTTAGCTACCGCGTACCCAAAACTTATCCTAG
TACACCGCAGATAGGCGTGCGCGTTAAAGTCCCCTTCGGTCGTCAACAATTGATCGGATTAGTCACAGGTATCACAGATA
CATGTAGCTTAGCGCCTAAGCAGATTAAGTCTGTGCTTGAGTTTATCGACGATAAACCTTTGTTGCCTGAATCACTTTAT
AAATTAACCCTTTGGGCCGCGAGATACTATTTTTGCAGCCAAGGGCAGATGCTAACCCAAGCCTTACCTGTCGCCCTACG
TAAAGGGCTCGATGCCGCGCCGCAAAAGATCCAATTCTGGCAATTAACTGAGATAGGCCAAAATATTGCGCCAGAAGCGG
TTAAGCGTGCACCCGCACAGAAAAAATTACTCGAGTTACTCAAACCCGCCAGCTTAACCCAAGAAGAAGTGATCAGCTTA
GATATCAGTAAAGCGGCACTGAAAGCCCTAGAAGATAAAGGCTGGGTGGCGCGCCACGAAAAAATCGCCGAGCTTAACCT
CGACTGGCGCACCACACTCGAAATGGACGAAACGCCACATAAACTCAATAAAGAACAAGCCGTCGCCGTTGCCACTTTAA
ATCTGCAACAAGGGTATCACTGTACTTTATTAGAAGGCATTACCGGTTCGGGTAAAACTGAAGTCTATTTGGCGGTGCTT
GAGGAAATCCTCAAGCAAGGCAAGCAAGCGTTAATTTTAGTGCCTGAAATCGGCTTGACGCCCCAGACAATCAATCGCTT
TAAGCGTCGTTTCAAAGTCAATGTCGCCATATTACACTCAGGGTTAACCGACAATCAAAGACTCGAAGCTTGGCGCCAAG
CCCGCTGTGGTCAGGCCGCAATCATTATCGGCACCCGCTCGGCCTTGTTCACGCCGATGGCCTATCCCGGCATCATCATA
CTCGATGAAGAACACGACAGTAGCTTCAAGCAACAGGAAGGCGTCGGATATCACGCCCGCGACTTGGCTGTCATGCGCGG
TCATTTAGAATCAATTCCGGTGATATTAGGTACGGCAACGCCCTCACTCGAAAGTCTACAAAATGCCTTAAGCGGCCGTT
ACCATCATTTGCAACTGGATGAGCGTGCAGGCGCAGCTAAAAAAGTGCGCCAAGGCATTATCGACATTAAAAATATGCCG
CTCAAAGCAGGAATGTCAGTACCGCTGCTGAACGAAATCCGCCTACATTTAGACGCAGGCAACCAAGTGTTACTGTTTCT
AAATCGCCGCGGCTTCGCACCAGCCCTACTTTGCCACGAGTGTGGGCACTTACACGAGTGCGACCGCTGCGACGCGTTTT
TTACTGTACATCAATCCCTTGGTGAAATTTGCTGCCACCATTGTGGCAATCAATATGCCATCCCTAAGCAATGCCACAAA
TGCGGCAGCACTATGCTGATGGGCCAAGGTATAGGCACAGAACAACTCGCCGAGGCACTGCAGCTGGAGTTTCCGAAACA
TCCAGTGGTACGCATCGACCGCGATACCACTCGCTTAAAAGGCTCACTAGAAACCCACTTAAACGCAATTCATAAGGGCG
AATATAAAATCCTCGTCGGCACGCAAATGCTGGCTAAAGGCCATCACTTCCCTGATGTGACACTTGTTGGGCTACTCGAT
GTCGATGGTGCCTTATTTAGCGCCGACTTCCGCGCGCCCGAGCGTTTTGGGCAACTCTACACCCAAGTCGCTGGTCGTGC
GGGCCGCGCCAGTAAACCCGGCACGGTATTATTGCAAACTCACCAGAGCGACAATCCCATTTTGCGGGATTTACTGCACC
GTGGTTATGGCGAGTTTGCTCGCAGCCAGCTAAAAGAGCGTCAACAAGCACTGCTGCCGCCAGCATGGCACATGGTGCTG
GTGCGCGCCGAAGCGCATTTAGCCAGTGATGCCGATAACTTTCTCAACGCCTTCGCCGCGCTTTTGCCCCAAGATAAAGA
ATTTGAGGTGATTGGCCCTATGCCAGCGCCCTTAGATAAAAAAGCCGGCAAGTTCCGTCGCCAACTGCTATTCCAAGCGA
AAAACCGCAACCGTCTGCAGCAAGAGTTTGAACGTATTCATCCGCTTGTAGAGCAGCTTATCGAAGCCAAGCGCTGCCGA
TGGAGCTTAGACAGAGATCCGCAGGATCTCCTCTGA

Upstream 100 bases:

>100_bases
ATTTACTCTCAACTATCCTTGGTAAACCATCATTAGGGCTAGCCCTAGCCAGCCGCGCTGAGTACACTAAACCACATTCT
TGCCTAAGACTTGCCAGCAT

Downstream 100 bases:

>100_bases
GTCGAGTATTTGACAATCATTTGATAAAAAATCATCGGGAAGATAGCAATTGTTAACCACAAACGGCTAAAATATGCGGT
TATTCCCTGTATTCACTCTT

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 731; Mature: 730

Protein sequence:

>731_residues
MSLFVEVALPVPMRQTFSYRVPKTYPSTPQIGVRVKVPFGRQQLIGLVTGITDTCSLAPKQIKSVLEFIDDKPLLPESLY
KLTLWAARYYFCSQGQMLTQALPVALRKGLDAAPQKIQFWQLTEIGQNIAPEAVKRAPAQKKLLELLKPASLTQEEVISL
DISKAALKALEDKGWVARHEKIAELNLDWRTTLEMDETPHKLNKEQAVAVATLNLQQGYHCTLLEGITGSGKTEVYLAVL
EEILKQGKQALILVPEIGLTPQTINRFKRRFKVNVAILHSGLTDNQRLEAWRQARCGQAAIIIGTRSALFTPMAYPGIII
LDEEHDSSFKQQEGVGYHARDLAVMRGHLESIPVILGTATPSLESLQNALSGRYHHLQLDERAGAAKKVRQGIIDIKNMP
LKAGMSVPLLNEIRLHLDAGNQVLLFLNRRGFAPALLCHECGHLHECDRCDAFFTVHQSLGEICCHHCGNQYAIPKQCHK
CGSTMLMGQGIGTEQLAEALQLEFPKHPVVRIDRDTTRLKGSLETHLNAIHKGEYKILVGTQMLAKGHHFPDVTLVGLLD
VDGALFSADFRAPERFGQLYTQVAGRAGRASKPGTVLLQTHQSDNPILRDLLHRGYGEFARSQLKERQQALLPPAWHMVL
VRAEAHLASDADNFLNAFAALLPQDKEFEVIGPMPAPLDKKAGKFRRQLLFQAKNRNRLQQEFERIHPLVEQLIEAKRCR
WSLDRDPQDLL

Sequences:

>Translated_731_residues
MSLFVEVALPVPMRQTFSYRVPKTYPSTPQIGVRVKVPFGRQQLIGLVTGITDTCSLAPKQIKSVLEFIDDKPLLPESLY
KLTLWAARYYFCSQGQMLTQALPVALRKGLDAAPQKIQFWQLTEIGQNIAPEAVKRAPAQKKLLELLKPASLTQEEVISL
DISKAALKALEDKGWVARHEKIAELNLDWRTTLEMDETPHKLNKEQAVAVATLNLQQGYHCTLLEGITGSGKTEVYLAVL
EEILKQGKQALILVPEIGLTPQTINRFKRRFKVNVAILHSGLTDNQRLEAWRQARCGQAAIIIGTRSALFTPMAYPGIII
LDEEHDSSFKQQEGVGYHARDLAVMRGHLESIPVILGTATPSLESLQNALSGRYHHLQLDERAGAAKKVRQGIIDIKNMP
LKAGMSVPLLNEIRLHLDAGNQVLLFLNRRGFAPALLCHECGHLHECDRCDAFFTVHQSLGEICCHHCGNQYAIPKQCHK
CGSTMLMGQGIGTEQLAEALQLEFPKHPVVRIDRDTTRLKGSLETHLNAIHKGEYKILVGTQMLAKGHHFPDVTLVGLLD
VDGALFSADFRAPERFGQLYTQVAGRAGRASKPGTVLLQTHQSDNPILRDLLHRGYGEFARSQLKERQQALLPPAWHMVL
VRAEAHLASDADNFLNAFAALLPQDKEFEVIGPMPAPLDKKAGKFRRQLLFQAKNRNRLQQEFERIHPLVEQLIEAKRCR
WSLDRDPQDLL
>Mature_730_residues
SLFVEVALPVPMRQTFSYRVPKTYPSTPQIGVRVKVPFGRQQLIGLVTGITDTCSLAPKQIKSVLEFIDDKPLLPESLYK
LTLWAARYYFCSQGQMLTQALPVALRKGLDAAPQKIQFWQLTEIGQNIAPEAVKRAPAQKKLLELLKPASLTQEEVISLD
ISKAALKALEDKGWVARHEKIAELNLDWRTTLEMDETPHKLNKEQAVAVATLNLQQGYHCTLLEGITGSGKTEVYLAVLE
EILKQGKQALILVPEIGLTPQTINRFKRRFKVNVAILHSGLTDNQRLEAWRQARCGQAAIIIGTRSALFTPMAYPGIIIL
DEEHDSSFKQQEGVGYHARDLAVMRGHLESIPVILGTATPSLESLQNALSGRYHHLQLDERAGAAKKVRQGIIDIKNMPL
KAGMSVPLLNEIRLHLDAGNQVLLFLNRRGFAPALLCHECGHLHECDRCDAFFTVHQSLGEICCHHCGNQYAIPKQCHKC
GSTMLMGQGIGTEQLAEALQLEFPKHPVVRIDRDTTRLKGSLETHLNAIHKGEYKILVGTQMLAKGHHFPDVTLVGLLDV
DGALFSADFRAPERFGQLYTQVAGRAGRASKPGTVLLQTHQSDNPILRDLLHRGYGEFARSQLKERQQALLPPAWHMVLV
RAEAHLASDADNFLNAFAALLPQDKEFEVIGPMPAPLDKKAGKFRRQLLFQAKNRNRLQQEFERIHPLVEQLIEAKRCRW
SLDRDPQDLL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=727, Percent_Identity=50.2063273727648, Blast_Score=714, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81785; Mature: 81654

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFVEVALPVPMRQTFSYRVPKTYPSTPQIGVRVKVPFGRQQLIGLVTGITDTCSLAPK
CCEEEEEECCCCCHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHCCHH
QIKSVLEFIDDKPLLPESLYKLTLWAARYYFCSQGQMLTQALPVALRKGLDAAPQKIQFW
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEE
QLTEIGQNIAPEAVKRAPAQKKLLELLKPASLTQEEVISLDISKAALKALEDKGWVARHE
EHHHHCCCCCCHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
KIAELNLDWRTTLEMDETPHKLNKEQAVAVATLNLQQGYHCTLLEGITGSGKTEVYLAVL
HHHHCCCCEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHH
EEILKQGKQALILVPEIGLTPQTINRFKRRFKVNVAILHSGLTDNQRLEAWRQARCGQAA
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHCCCCCEE
IIIGTRSALFTPMAYPGIIILDEEHDSSFKQQEGVGYHARDLAVMRGHLESIPVILGTAT
EEEECCHHHHCCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCC
PSLESLQNALSGRYHHLQLDERAGAAKKVRQGIIDIKNMPLKAGMSVPLLNEIRLHLDAG
CCHHHHHHHHCCCEEEEEEHHHCCHHHHHHHHHHHHCCCCHHCCCCCCCHHHEEEEEECC
NQVLLFLNRRGFAPALLCHECGHLHECDRCDAFFTVHQSLGEICCHHCGNQYAIPKQCHK
CEEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
CGSTMLMGQGIGTEQLAEALQLEFPKHPVVRIDRDTTRLKGSLETHLNAIHKGEYKILVG
CCCCEEECCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHCCHHHHHHHHHCCCEEEEEE
TQMLAKGHHFPDVTLVGLLDVDGALFSADFRAPERFGQLYTQVAGRAGRASKPGTVLLQT
HHHHHCCCCCCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEE
HQSDNPILRDLLHRGYGEFARSQLKERQQALLPPAWHMVLVRAEAHLASDADNFLNAFAA
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHEEEEEHHHHHHCCHHHHHHHHHH
LLPQDKEFEVIGPMPAPLDKKAGKFRRQLLFQAKNRNRLQQEFERIHPLVEQLIEAKRCR
HCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
WSLDRDPQDLL
CCCCCCHHHCC
>Mature Secondary Structure 
SLFVEVALPVPMRQTFSYRVPKTYPSTPQIGVRVKVPFGRQQLIGLVTGITDTCSLAPK
CEEEEEECCCCCHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHCCHH
QIKSVLEFIDDKPLLPESLYKLTLWAARYYFCSQGQMLTQALPVALRKGLDAAPQKIQFW
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEE
QLTEIGQNIAPEAVKRAPAQKKLLELLKPASLTQEEVISLDISKAALKALEDKGWVARHE
EHHHHCCCCCCHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
KIAELNLDWRTTLEMDETPHKLNKEQAVAVATLNLQQGYHCTLLEGITGSGKTEVYLAVL
HHHHCCCCEEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHH
EEILKQGKQALILVPEIGLTPQTINRFKRRFKVNVAILHSGLTDNQRLEAWRQARCGQAA
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHCCCCCEE
IIIGTRSALFTPMAYPGIIILDEEHDSSFKQQEGVGYHARDLAVMRGHLESIPVILGTAT
EEEECCHHHHCCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCC
PSLESLQNALSGRYHHLQLDERAGAAKKVRQGIIDIKNMPLKAGMSVPLLNEIRLHLDAG
CCHHHHHHHHCCCEEEEEEHHHCCHHHHHHHHHHHHCCCCHHCCCCCCCHHHEEEEEECC
NQVLLFLNRRGFAPALLCHECGHLHECDRCDAFFTVHQSLGEICCHHCGNQYAIPKQCHK
CEEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
CGSTMLMGQGIGTEQLAEALQLEFPKHPVVRIDRDTTRLKGSLETHLNAIHKGEYKILVG
CCCCEEECCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHCCHHHHHHHHHCCCEEEEEE
TQMLAKGHHFPDVTLVGLLDVDGALFSADFRAPERFGQLYTQVAGRAGRASKPGTVLLQT
HHHHHCCCCCCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEE
HQSDNPILRDLLHRGYGEFARSQLKERQQALLPPAWHMVLVRAEAHLASDADNFLNAFAA
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHEEEEEHHHHHHCCHHHHHHHHHH
LLPQDKEFEVIGPMPAPLDKKAGKFRRQLLFQAKNRNRLQQEFERIHPLVEQLIEAKRCR
HCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
WSLDRDPQDLL
CCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]