Click here to switch to the map view.

The map label for this gene is ada [H]

Identifier: 126175667

GI number: 126175667

Start: 4071933

End: 4072775

Strand: Reverse

Name: ada [H]

Synonym: Sbal_3470

Alternate gene names: 126175667

Gene position: 4072775-4071933 (Counterclockwise)

Preceding gene: 126175668

Following gene: 126175666

Centisome position: 79.43

GC content: 51.01

Gene sequence:

>843_bases
ATGTCAGACTATGACCGAATCGCAAGCGCTATGGCCTATTTAGTTGATCGGGCAATAGATCAGCCCAGCCTTGAAGAGAT
AGCCGCCCATGTGCATCTGAGTCCGTATCACTTTCAAAGGCTGTTTTGTCGTTGGGCAGGTACTACGCCGAAGCGCTTTT
TACAGGTACTGACATTGGAGCGGGGTAAAGGATTGCTTAATAATTCTCGCTCCTTGCTTGATGTCTCACATGAGCTTGGA
TTAAGTGGCAGTTCAAGGTTGCATGATCACTTTGTGCAGCTGGAAGCAGTCACACCCGGCGAGTATAAAAATCGAGGTAA
GGACGTACAGATCGAGTATGGAGTCCATCCTACCCCACTGGGGCCCATGTTTGTGGCCGTAACCCTGCGTGGTGTTTGCC
GGGCAGCGTTTATGGACTTCAATAGCATGGAAGAGTTACTGAATGACTTGTATAACGATTGGCCTCTCAGTTCGATCAGA
GAAAATTTGGCATCGACGCGACACGCTATTGATGCCTTTTTTAGCAGTGAAACAGCTTCCGGGCAAGGTCCGCTGTCGCT
CCATGTGACAGGGACAAATTTCCAGATCGCCGTGTGGCGTGCTTTGTTGAAAATACCACCGGGCGCTGTGGCCAGCTATG
CCGAAGTAGCCAGATCACTCGGCGCACCCAGGTCTGCAAGGGCTGTTGGTAACGCTATTGGAGCCAATCCAGTTGCTTTG
CTGATCCCCTGTCACCGGGTTATACAGCAAAGTGGCGCGCTGGGGGGGTATCGCTGGGGACCAACCAAGAAGCTTATGGT
TCAAACATGGGAAAAAATGCGTGATGAACCGGTTGCGCTATAA

Upstream 100 bases:

>100_bases
CTGCATTGTACCTTGCCTCCGACGCCTCCAGCTTCACCACGGGATCTGCAATGCTGGTCGATGGCGGTGTATCTATAAAT
CGTGTTTGAGAGGCCGCCCC

Downstream 100 bases:

>100_bases
CAATGCCATGCAACCGACGCTCGAACCTCGCTCGACTGATGGTGAGCGTTACCCATAGAAATCTTAATCCGAAAGTTACA
TATCAGAGTATTCGCACTAA

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSDYDRIASAMAYLVDRAIDQPSLEEIAAHVHLSPYHFQRLFCRWAGTTPKRFLQVLTLERGKGLLNNSRSLLDVSHELG
LSGSSRLHDHFVQLEAVTPGEYKNRGKDVQIEYGVHPTPLGPMFVAVTLRGVCRAAFMDFNSMEELLNDLYNDWPLSSIR
ENLASTRHAIDAFFSSETASGQGPLSLHVTGTNFQIAVWRALLKIPPGAVASYAEVARSLGAPRSARAVGNAIGANPVAL
LIPCHRVIQQSGALGGYRWGPTKKLMVQTWEKMRDEPVAL

Sequences:

>Translated_280_residues
MSDYDRIASAMAYLVDRAIDQPSLEEIAAHVHLSPYHFQRLFCRWAGTTPKRFLQVLTLERGKGLLNNSRSLLDVSHELG
LSGSSRLHDHFVQLEAVTPGEYKNRGKDVQIEYGVHPTPLGPMFVAVTLRGVCRAAFMDFNSMEELLNDLYNDWPLSSIR
ENLASTRHAIDAFFSSETASGQGPLSLHVTGTNFQIAVWRALLKIPPGAVASYAEVARSLGAPRSARAVGNAIGANPVAL
LIPCHRVIQQSGALGGYRWGPTKKLMVQTWEKMRDEPVAL
>Mature_279_residues
SDYDRIASAMAYLVDRAIDQPSLEEIAAHVHLSPYHFQRLFCRWAGTTPKRFLQVLTLERGKGLLNNSRSLLDVSHELGL
SGSSRLHDHFVQLEAVTPGEYKNRGKDVQIEYGVHPTPLGPMFVAVTLRGVCRAAFMDFNSMEELLNDLYNDWPLSSIRE
NLASTRHAIDAFFSSETASGQGPLSLHVTGTNFQIAVWRALLKIPPGAVASYAEVARSLGAPRSARAVGNAIGANPVALL
IPCHRVIQQSGALGGYRWGPTKKLMVQTWEKMRDEPVAL

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=73, Percent_Identity=46.5753424657534, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1788542, Length=255, Percent_Identity=30.9803921568627, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787596, Length=161, Percent_Identity=37.2670807453416, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115533068, Length=79, Percent_Identity=45.5696202531646, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=79, Percent_Identity=45.5696202531646, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=93, Percent_Identity=40.8602150537634, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI17137554, Length=128, Percent_Identity=34.375, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 30877; Mature: 30745

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYDRIASAMAYLVDRAIDQPSLEEIAAHVHLSPYHFQRLFCRWAGTTPKRFLQVLTLE
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
RGKGLLNNSRSLLDVSHELGLSGSSRLHDHFVQLEAVTPGEYKNRGKDVQIEYGVHPTPL
HCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHEEEEEECCCHHHCCCCCEEEEECCCCCCC
GPMFVAVTLRGVCRAAFMDFNSMEELLNDLYNDWPLSSIRENLASTRHAIDAFFSSETAS
CHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
GQGPLSLHVTGTNFQIAVWRALLKIPPGAVASYAEVARSLGAPRSARAVGNAIGANPVAL
CCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEE
LIPCHRVIQQSGALGGYRWGPTKKLMVQTWEKMRDEPVAL
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDYDRIASAMAYLVDRAIDQPSLEEIAAHVHLSPYHFQRLFCRWAGTTPKRFLQVLTLE
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
RGKGLLNNSRSLLDVSHELGLSGSSRLHDHFVQLEAVTPGEYKNRGKDVQIEYGVHPTPL
HCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHEEEEEECCCHHHCCCCCEEEEECCCCCCC
GPMFVAVTLRGVCRAAFMDFNSMEELLNDLYNDWPLSSIRENLASTRHAIDAFFSSETAS
CHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
GQGPLSLHVTGTNFQIAVWRALLKIPPGAVASYAEVARSLGAPRSARAVGNAIGANPVAL
CCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEE
LIPCHRVIQQSGALGGYRWGPTKKLMVQTWEKMRDEPVAL
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1904855; 11677609 [H]