Click here to switch to the map view.

The map label for this gene is lipB [H]

Identifier: 126175481

GI number: 126175481

Start: 3843227

End: 3843880

Strand: Direct

Name: lipB [H]

Synonym: Sbal_3281

Alternate gene names: 126175481

Gene position: 3843227-3843880 (Clockwise)

Preceding gene: 126175480

Following gene: 126175482

Centisome position: 74.96

GC content: 49.24

Gene sequence:

>654_bases
TTGCAAGACACGACTTTGCACATTCGACATCTTGGAAAACAAGATTACGAATCAGTGTGGCATGCGATGCAGCATTACAC
TGATACGCGCGACAGCGAAAGCCATGATGAACTGTGGATTGTCGAGCATCCGCCCGTGTTTACCCAAGGCCAAGCCGGCA
AAAGCGAGCATATTTTAAATGCTGGCGATATTCCGGTTATCCAAGTGGACCGCGGCGGTCAAGTCACTTACCACGGCCCA
GGCCAACTAGTGGTATATCCTCTTCTCGATATAAAACGTAGTAAAGTCGGCGTACGTCAACTCGTCACCCATATAGAGCA
AAGCATTGTCGATATGCTCGCCAAGTATGACATTAACGCTTATGCCAAGGCCGATGCGCCGGGCGTATATGTGGATGAAC
GCAAAATCGCCTCCCTAGGATTACGCATTCGTAAAGGCTGTTCATTCCATGGATTAGCCCTTAATGTCGACATGGATCTG
GCGCCATTTCGCCGCATCAACCCTTGTGGTTATGCCGGTCTTGAAATGGTGCAATGCAAAGCGCTTGGCGGACCACAAAC
CGTGATTGAAGCGGGCGAGCAACTCACCATTACTTTTAGCCAATTACTGGGCTATCAACACCTAGTTCATCATCAAGGAT
TAGCAGCGTCATGA

Upstream 100 bases:

>100_bases
TACTATAAATATTCCTCAAGGCCATGAAATGTGATCTGTCTTACATTTATGGCCAATAAGCGTATAATAGCCCACTCATT
TCTAAGGGGAGAGGTTGCCC

Downstream 100 bases:

>100_bases
ATAGGCCTGAACGTTTACAACCCGGAGTAAAATTAAGAGATGCCGATAAAGTGTCTCGTATTCCGGTCAAAATCGTCCCC
TCAGAGCGCGACACTATGTT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MQDTTLHIRHLGKQDYESVWHAMQHYTDTRDSESHDELWIVEHPPVFTQGQAGKSEHILNAGDIPVIQVDRGGQVTYHGP
GQLVVYPLLDIKRSKVGVRQLVTHIEQSIVDMLAKYDINAYAKADAPGVYVDERKIASLGLRIRKGCSFHGLALNVDMDL
APFRRINPCGYAGLEMVQCKALGGPQTVIEAGEQLTITFSQLLGYQHLVHHQGLAAS

Sequences:

>Translated_217_residues
MQDTTLHIRHLGKQDYESVWHAMQHYTDTRDSESHDELWIVEHPPVFTQGQAGKSEHILNAGDIPVIQVDRGGQVTYHGP
GQLVVYPLLDIKRSKVGVRQLVTHIEQSIVDMLAKYDINAYAKADAPGVYVDERKIASLGLRIRKGCSFHGLALNVDMDL
APFRRINPCGYAGLEMVQCKALGGPQTVIEAGEQLTITFSQLLGYQHLVHHQGLAAS
>Mature_217_residues
MQDTTLHIRHLGKQDYESVWHAMQHYTDTRDSESHDELWIVEHPPVFTQGQAGKSEHILNAGDIPVIQVDRGGQVTYHGP
GQLVVYPLLDIKRSKVGVRQLVTHIEQSIVDMLAKYDINAYAKADAPGVYVDERKIASLGLRIRKGCSFHGLALNVDMDL
APFRRINPCGYAGLEMVQCKALGGPQTVIEAGEQLTITFSQLLGYQHLVHHQGLAAS

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=136, Percent_Identity=37.5, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI87081767, Length=177, Percent_Identity=68.361581920904, Blast_Score=256, Evalue=6e-70,
Organism=Saccharomyces cerevisiae, GI6323268, Length=117, Percent_Identity=36.7521367521368, Blast_Score=79, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21355483, Length=192, Percent_Identity=30.2083333333333, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 24100; Mature: 24100

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDTTLHIRHLGKQDYESVWHAMQHYTDTRDSESHDELWIVEHPPVFTQGQAGKSEHILN
CCCCEEHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEC
AGDIPVIQVDRGGQVTYHGPGQLVVYPLLDIKRSKVGVRQLVTHIEQSIVDMLAKYDINA
CCCCCEEEECCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
YAKADAPGVYVDERKIASLGLRIRKGCSFHGLALNVDMDLAPFRRINPCGYAGLEMVQCK
EECCCCCCEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEE
ALGGPQTVIEAGEQLTITFSQLLGYQHLVHHQGLAAS
CCCCCHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQDTTLHIRHLGKQDYESVWHAMQHYTDTRDSESHDELWIVEHPPVFTQGQAGKSEHILN
CCCCEEHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEC
AGDIPVIQVDRGGQVTYHGPGQLVVYPLLDIKRSKVGVRQLVTHIEQSIVDMLAKYDINA
CCCCCEEEECCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
YAKADAPGVYVDERKIASLGLRIRKGCSFHGLALNVDMDLAPFRRINPCGYAGLEMVQCK
EECCCCCCEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEE
ALGGPQTVIEAGEQLTITFSQLLGYQHLVHHQGLAAS
CCCCCHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]