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The map label for this gene is lipA [H]

Identifier: 126175482

GI number: 126175482

Start: 3843877

End: 3844842

Strand: Direct

Name: lipA [H]

Synonym: Sbal_3282

Alternate gene names: 126175482

Gene position: 3843877-3844842 (Clockwise)

Preceding gene: 126175481

Following gene: 126175485

Centisome position: 74.97

GC content: 48.76

Gene sequence:

>966_bases
ATGAATAGGCCTGAACGTTTACAACCCGGAGTAAAATTAAGAGATGCCGATAAAGTGTCTCGTATTCCGGTCAAAATCGT
CCCCTCAGAGCGCGACACTATGTTGCGTAAACCCGATTGGTTACGGGTTAAACTGCCGGCATCGAATCAACGCATTTTAG
AGATCAAGCAAGCGCTGCGTAAAAATGGCCTGCACTCTGTCTGTGAAGAGGCATCTTGCCCTAACCTCGCAGAATGCTTT
AACCACGGCACTGCGACCTTTATGATTTTGGGTGCGATTTGTACACGTCGTTGCCCATTCTGCGACGTAGCCCATGGTCG
TCCGCTTAAGCCTGACGCTGAAGAGCCGGTTAAATTGGCGCAAACAATCCGCGATATGAAGCTGAAATACGTGGTCATTA
CCTCGGTAGATCGTGACGATTTACGTGATGGTGGCGCCCAGCATTTTGCCGATTGTATCCGCGAAATTCGTAAACTGAAT
CCAGACATCAAGATCGAAACCTTAGTACCTGATTTCCGTGGTCGTATCGATGCTGCCTTAGATATTTTATCGACTGAGCC
GCCTGATGTGTTCAACCACAACTTAGAAACGGCGCCAATGCATTATCGTAAAGCGCGCCCAGGTGCGAATTACCAATGGT
CGCTCGATCTGCTAAAACGCTTTAAAGAACGTCATCCAAATGTGCCAACTAAGTCAGGCCTGATGATGGGACTGGGTGAG
ACCAACGAAGAAATCGCTCAAGTATTACGTGACTTGCGCGCCCACAACGTTGAAATGCTGACCTTAGGCCAATACCTACA
GCCGTCTAAGTTCCACTTGCCGGTTGAACGTTATGTGCCACCAGCAGAGTTTGACGAGCTTAAAGCCTTAGCCGATGAGC
TAGGTTTTACCCATGCAGCCTGTGGCCCATTAGTGCGCTCAAGCTACCATGCAGACTTGCAGGCCCAAGGTAAAGAAGTG
AAATAA

Upstream 100 bases:

>100_bases
GACCACAAACCGTGATTGAAGCGGGCGAGCAACTCACCATTACTTTTAGCCAATTACTGGGCTATCAACACCTAGTTCAT
CATCAAGGATTAGCAGCGTC

Downstream 100 bases:

>100_bases
CCTTCTTGCTCAGATGCAGATAGCCTTGATGCAAACTTCAGGTGATGCTGAATTTAAGTGATATAGAGTTCAGGGTTATC
CAAATAAAAAAACAGCGCTT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNRPERLQPGVKLRDADKVSRIPVKIVPSERDTMLRKPDWLRVKLPASNQRILEIKQALRKNGLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPLKPDAEEPVKLAQTIRDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRKLN
PDIKIETLVPDFRGRIDAALDILSTEPPDVFNHNLETAPMHYRKARPGANYQWSLDLLKRFKERHPNVPTKSGLMMGLGE
TNEEIAQVLRDLRAHNVEMLTLGQYLQPSKFHLPVERYVPPAEFDELKALADELGFTHAACGPLVRSSYHADLQAQGKEV
K

Sequences:

>Translated_321_residues
MNRPERLQPGVKLRDADKVSRIPVKIVPSERDTMLRKPDWLRVKLPASNQRILEIKQALRKNGLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPLKPDAEEPVKLAQTIRDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRKLN
PDIKIETLVPDFRGRIDAALDILSTEPPDVFNHNLETAPMHYRKARPGANYQWSLDLLKRFKERHPNVPTKSGLMMGLGE
TNEEIAQVLRDLRAHNVEMLTLGQYLQPSKFHLPVERYVPPAEFDELKALADELGFTHAACGPLVRSSYHADLQAQGKEV
K
>Mature_321_residues
MNRPERLQPGVKLRDADKVSRIPVKIVPSERDTMLRKPDWLRVKLPASNQRILEIKQALRKNGLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPLKPDAEEPVKLAQTIRDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRKLN
PDIKIETLVPDFRGRIDAALDILSTEPPDVFNHNLETAPMHYRKARPGANYQWSLDLLKRFKERHPNVPTKSGLMMGLGE
TNEEIAQVLRDLRAHNVEMLTLGQYLQPSKFHLPVERYVPPAEFDELKALADELGFTHAACGPLVRSSYHADLQAQGKEV
K

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=284, Percent_Identity=45.0704225352113, Blast_Score=248, Evalue=8e-66,
Organism=Homo sapiens, GI37577164, Length=248, Percent_Identity=44.3548387096774, Blast_Score=209, Evalue=3e-54,
Organism=Escherichia coli, GI1786846, Length=321, Percent_Identity=75.7009345794392, Blast_Score=518, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI32564533, Length=305, Percent_Identity=41.6393442622951, Blast_Score=226, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=283, Percent_Identity=45.9363957597173, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=45.4545454545455, Blast_Score=244, Evalue=7e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36376; Mature: 36376

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRPERLQPGVKLRDADKVSRIPVKIVPSERDTMLRKPDWLRVKLPASNQRILEIKQALR
CCCCCCCCCCCEECCCCHHHCCCEEEECCCCHHHCCCCCEEEEECCCCCHHHHHHHHHHH
KNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPLKPDAEEPVKLA
HCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
QTIRDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRKLNPDIKIETLVPDFRGRIDAAL
HHHHHCCEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHH
DILSTEPPDVFNHNLETAPMHYRKARPGANYQWSLDLLKRFKERHPNVPTKSGLMMGLGE
HHHCCCCCHHHCCCCCCCCCHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCCCCEEECCCC
TNEEIAQVLRDLRAHNVEMLTLGQYLQPSKFHLPVERYVPPAEFDELKALADELGFTHAA
CHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCHHHH
CGPLVRSSYHADLQAQGKEVK
HHHHHHHCCCCCHHCCCCCCC
>Mature Secondary Structure
MNRPERLQPGVKLRDADKVSRIPVKIVPSERDTMLRKPDWLRVKLPASNQRILEIKQALR
CCCCCCCCCCCEECCCCHHHCCCEEEECCCCHHHCCCCCEEEEECCCCCHHHHHHHHHHH
KNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPLKPDAEEPVKLA
HCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
QTIRDMKLKYVVITSVDRDDLRDGGAQHFADCIREIRKLNPDIKIETLVPDFRGRIDAAL
HHHHHCCEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHH
DILSTEPPDVFNHNLETAPMHYRKARPGANYQWSLDLLKRFKERHPNVPTKSGLMMGLGE
HHHCCCCCHHHCCCCCCCCCHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCCCCEEECCCC
TNEEIAQVLRDLRAHNVEMLTLGQYLQPSKFHLPVERYVPPAEFDELKALADELGFTHAA
CHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCHHHH
CGPLVRSSYHADLQAQGKEVK
HHHHHHHCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA