Click here to switch to the map view.

The map label for this gene is rumA [H]

Identifier: 126175348

GI number: 126175348

Start: 3684955

End: 3686307

Strand: Reverse

Name: rumA [H]

Synonym: Sbal_3147

Alternate gene names: 126175348

Gene position: 3686307-3684955 (Counterclockwise)

Preceding gene: 126175352

Following gene: 126175347

Centisome position: 71.89

GC content: 48.71

Gene sequence:

>1353_bases
ATGGCGCAATTTTTTAAAGCAAAACCAAATAGTTCCAAACAGTTGTCGGCTAAGTTATCGCTGAGCGTGAATCAGCTCGA
TCACTTAGGGGCAGGTATAGCACAACATCAAGGTAAAGTCGTGTTTATCCCCGGCGCACTGCCCGATGAAACCGTGACGG
TACAGTTCACCGAGCAAAAGAAAAATTATGCCCGCGCTAAGTTAATTAAAGTCGATACACCTAGCTCTGAGCGCGTCGAG
CCTGAGTGTCCACACTATCACACCTGCGGCGGCTGCGACTTACAGCATATGTCATTATCGGGCCAGCGTGAGCATAAAGA
AGCGGCGCTGCTGGATATTATGGCTAAGTTTGCCGGCGCCGAAGGCGGCACGTTATCGCCCGAATTAACGGGGGAAGGTT
GGCATTATCGTCGCCGTGCCCGTCTTGCGACTTTATTTGATAAAAATACTAAACACTTAAGCTTAGGTTTTCGTGCCGCT
AGTAGCAGCAATGTGGTGCCTATCAGTCAGTGTCAGGTGTTAGCTAAGCCGTTATCAGATTTGATCGTCCCCTTTGCTAA
GTTGCTGAATCAATTAACGGCTAAAGCCAGCTTAGGTCACCTTGAGTTAATTGCCGCCGATAATGGTCACTTCGCTGTGC
TGCGTATCACTAAGGCGCTCAACGATAAAGATCTCGCTAAGCTGTCAGCCTTTGCCGAGCAGCATCAAATTCACATTTGT
CTGCAGGATAATGAAGGCCAATTCCAAGGCGTTGGCGCAGAGTTAGTGTTACCGGTTTATCAGCTGTTAGATGACAAAGC
TGAGTCGGATGCGGTGAGTTTAAGCTTCACCCCAGGTAACTTTGTGCAAGTGAATGGGCAGATTAACAAGGCCATGGTCG
CCCAAGCGATGGATTGGTTAGCGCCAGCGCTAGATGAGCGCATTTTGGATTTGTTCTGCGGTATGGGGAATTTTAGTTTG
CCCTTAGCGAAAATGGGCGCGGATGTGATTGGCGTCGAAGGGGTTGCCGAGATGGTAACTCAGGCACGGGTGAACGCGAA
AGCTAACAATTTGGATAAACTGACCTTTTTCCACGGCGATTTAAGTGCGGATTTATCCCTTGAGCCTTGGATGGGCAAAA
TTGATAAATTACTGCTCGATCCTGCCCGTGCTGGGGCGTTTGAAAGCTTGCAATGGCTGAAGAAAATGAAGCCGCGCAAA
GTCGTGTATGTTTCGTGTAATCCTGCGAGCTTGGCCCGCGATAGCGCTGTACTGTTAGAGCGCGGTTACCGATTACAGCA
ATTGGGACTTATCGACATGTTCCCACAAACACACCATATCGAAGCCATGGCACTGTTTGAATTAACCAAGTAA

Upstream 100 bases:

>100_bases
AGCAGCGGCGGTGTTTCCTGTGACAGAGACTAAGATAATTTTATAAGACTGCCAGAAAGAGGGCGGTCTTTGCCAGTATT
TTATTGATTTAGAGGCCGAA

Downstream 100 bases:

>100_bases
TTTGCGCTGGCAAAGTAAGCAAAGCGTTTGAATAACGTAAAAAATGTGATTATTACGTCGGTTGGGTTTGACAAGTTCGC
GCCAATGCTGAAGATAGGAG

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MAQFFKAKPNSSKQLSAKLSLSVNQLDHLGAGIAQHQGKVVFIPGALPDETVTVQFTEQKKNYARAKLIKVDTPSSERVE
PECPHYHTCGGCDLQHMSLSGQREHKEAALLDIMAKFAGAEGGTLSPELTGEGWHYRRRARLATLFDKNTKHLSLGFRAA
SSSNVVPISQCQVLAKPLSDLIVPFAKLLNQLTAKASLGHLELIAADNGHFAVLRITKALNDKDLAKLSAFAEQHQIHIC
LQDNEGQFQGVGAELVLPVYQLLDDKAESDAVSLSFTPGNFVQVNGQINKAMVAQAMDWLAPALDERILDLFCGMGNFSL
PLAKMGADVIGVEGVAEMVTQARVNAKANNLDKLTFFHGDLSADLSLEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRK
VVYVSCNPASLARDSAVLLERGYRLQQLGLIDMFPQTHHIEAMALFELTK

Sequences:

>Translated_450_residues
MAQFFKAKPNSSKQLSAKLSLSVNQLDHLGAGIAQHQGKVVFIPGALPDETVTVQFTEQKKNYARAKLIKVDTPSSERVE
PECPHYHTCGGCDLQHMSLSGQREHKEAALLDIMAKFAGAEGGTLSPELTGEGWHYRRRARLATLFDKNTKHLSLGFRAA
SSSNVVPISQCQVLAKPLSDLIVPFAKLLNQLTAKASLGHLELIAADNGHFAVLRITKALNDKDLAKLSAFAEQHQIHIC
LQDNEGQFQGVGAELVLPVYQLLDDKAESDAVSLSFTPGNFVQVNGQINKAMVAQAMDWLAPALDERILDLFCGMGNFSL
PLAKMGADVIGVEGVAEMVTQARVNAKANNLDKLTFFHGDLSADLSLEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRK
VVYVSCNPASLARDSAVLLERGYRLQQLGLIDMFPQTHHIEAMALFELTK
>Mature_449_residues
AQFFKAKPNSSKQLSAKLSLSVNQLDHLGAGIAQHQGKVVFIPGALPDETVTVQFTEQKKNYARAKLIKVDTPSSERVEP
ECPHYHTCGGCDLQHMSLSGQREHKEAALLDIMAKFAGAEGGTLSPELTGEGWHYRRRARLATLFDKNTKHLSLGFRAAS
SSNVVPISQCQVLAKPLSDLIVPFAKLLNQLTAKASLGHLELIAADNGHFAVLRITKALNDKDLAKLSAFAEQHQIHICL
QDNEGQFQGVGAELVLPVYQLLDDKAESDAVSLSFTPGNFVQVNGQINKAMVAQAMDWLAPALDERILDLFCGMGNFSLP
LAKMGADVIGVEGVAEMVTQARVNAKANNLDKLTFFHGDLSADLSLEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRKV
VYVSCNPASLARDSAVLLERGYRLQQLGLIDMFPQTHHIEAMALFELTK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=290, Percent_Identity=29.6551724137931, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI51173878, Length=290, Percent_Identity=29.6551724137931, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789148, Length=452, Percent_Identity=42.9203539823009, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1787083, Length=177, Percent_Identity=32.2033898305085, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790403, Length=183, Percent_Identity=27.8688524590164, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24666579, Length=198, Percent_Identity=32.8282828282828, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 49243; Mature: 49112

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFFKAKPNSSKQLSAKLSLSVNQLDHLGAGIAQHQGKVVFIPGALPDETVTVQFTEQK
CCCCCCCCCCCCCEEEEEEEEEHHHHHHHCCCHHHHCCCEEEECCCCCCCEEEEEEECCC
KNYARAKLIKVDTPSSERVEPECPHYHTCGGCDLQHMSLSGQREHKEAALLDIMAKFAGA
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCC
EGGTLSPELTGEGWHYRRRARLATLFDKNTKHLSLGFRAASSSNVVPISQCQVLAKPLSD
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEHHHHHHHHHHHHH
LIVPFAKLLNQLTAKASLGHLELIAADNGHFAVLRITKALNDKDLAKLSAFAEQHQIHIC
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHCCEEEEE
LQDNEGQFQGVGAELVLPVYQLLDDKAESDAVSLSFTPGNFVQVNGQINKAMVAQAMDWL
EECCCCCEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHH
APALDERILDLFCGMGNFSLPLAKMGADVIGVEGVAEMVTQARVNAKANNLDKLTFFHGD
HHHHHHHHHHHHHCCCCCCCCHHHHCCCEECHHHHHHHHHHHHHCCCCCCCCEEEEEECC
LSADLSLEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRKVVYVSCNPASLARDSAVLLE
CCCCCCCCCHHHHHHHHHHCCHHCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHHH
RGYRLQQLGLIDMFPQTHHIEAMALFELTK
CCCCCHHCCHHHCCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
AQFFKAKPNSSKQLSAKLSLSVNQLDHLGAGIAQHQGKVVFIPGALPDETVTVQFTEQK
CCCCCCCCCCCCEEEEEEEEEHHHHHHHCCCHHHHCCCEEEECCCCCCCEEEEEEECCC
KNYARAKLIKVDTPSSERVEPECPHYHTCGGCDLQHMSLSGQREHKEAALLDIMAKFAGA
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCC
EGGTLSPELTGEGWHYRRRARLATLFDKNTKHLSLGFRAASSSNVVPISQCQVLAKPLSD
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEHHHHHHHHHHHHH
LIVPFAKLLNQLTAKASLGHLELIAADNGHFAVLRITKALNDKDLAKLSAFAEQHQIHIC
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHCCEEEEE
LQDNEGQFQGVGAELVLPVYQLLDDKAESDAVSLSFTPGNFVQVNGQINKAMVAQAMDWL
EECCCCCEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHH
APALDERILDLFCGMGNFSLPLAKMGADVIGVEGVAEMVTQARVNAKANNLDKLTFFHGD
HHHHHHHHHHHHHCCCCCCCCHHHHCCCEECHHHHHHHHHHHHHCCCCCCCCEEEEEECC
LSADLSLEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRKVVYVSCNPASLARDSAVLLE
CCCCCCCCCHHHHHHHHHHCCHHCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHHH
RGYRLQQLGLIDMFPQTHHIEAMALFELTK
CCCCCHHCCHHHCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA