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The map label for this gene is rfbD [C]

Identifier: 126175091

GI number: 126175091

Start: 3399818

End: 3400693

Strand: Reverse

Name: rfbD [C]

Synonym: Sbal_2888

Alternate gene names: 126175091

Gene position: 3400693-3399818 (Counterclockwise)

Preceding gene: 126175092

Following gene: 126175090

Centisome position: 66.32

GC content: 43.15

Gene sequence:

>876_bases
ATGAAAATATTAGTCACCGGTAGTAATGGCCAAGTGGGTAGTTGCCTCGTTAAACAATTGAGCCAGATGCCAGAGATTGA
ATTTTGGGCGGTTGATCGTACTCAATTAGATATAACAGATGCTGCTGCTGTCGCCAAGTTAGTCAATGACTTTAAACCTC
ATGCGATCATCAATGCGGCTGCGCACACTGCAGTAGATAAAGCAGAAGATGAAGTTGCGCTTTCCTATGCGATCAACCGT
GATGGCCCTCAATGGCTAGCCGAAGCAGCAAATAGTGCCGGCGCAGTTATGTTGCATATTTCCACTGACTATGTTTTTGC
GGGAGATAAACAGGGCGAATATCGAGAGACTGATGCGATTGATCCCCAAGGAGTCTATGGTAAAAGCAAACTGGCTGGTG
AGTTAGCCGTTGCAGCCGCTTGCCCCCGACATATAATTTTACGGACTGCTTGGGTATTTTGCGAAACTGGCAATAATTTT
GTTAAAACCATGCTTCGTCTTTCGCAATCTCGGGACGAATTAGGCGTGGTAGCAGATCAGTTCGGTGGCCCCACTTATGC
TGGTGATATTGCTAATGCGTTAATTACCATGGCTAAAGCCATTAGTAATGGCAATCAATCATTTGGCATCTATCATTACT
CTGGCTTACCTCATGTAAATTGGCACTTATTTGCTTGTGAGATTTTCAAGCAAGCTAAATCACAAGGTTTACTTGATAAA
GAAATGAATGTTAAAGCCATTACTAGCGAACAGTACCCAACACCAGCTAAGCGGCCGAAAAACTCTAAATTGGATTGTAA
TAAGATTCTACAACACTTTGGTATTCAAGCGAGTGATTGGCGAGCGGCATTGAATAAGTTGAATGGCTATTTATGA

Upstream 100 bases:

>100_bases
CGCCAAGCCGATGCTTAAAAATGACTATGGCCAGTATTTGGCCAAACTAGTGTAAGTGGTTAATAGTTAATGGTAAGTGA
AACACAAATAACGAATAATT

Downstream 100 bases:

>100_bases
GAGGTTGTGCTTAGTGTTTGTTGGCAATAGTTCAAAGTTCATAATTAAACCCCAACACTCAGACTTTAATAATCCGTAAC
TTAAGACTTATGAATCTCGA

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MKILVTGSNGQVGSCLVKQLSQMPEIEFWAVDRTQLDITDAAAVAKLVNDFKPHAIINAAAHTAVDKAEDEVALSYAINR
DGPQWLAEAANSAGAVMLHISTDYVFAGDKQGEYRETDAIDPQGVYGKSKLAGELAVAAACPRHIILRTAWVFCETGNNF
VKTMLRLSQSRDELGVVADQFGGPTYAGDIANALITMAKAISNGNQSFGIYHYSGLPHVNWHLFACEIFKQAKSQGLLDK
EMNVKAITSEQYPTPAKRPKNSKLDCNKILQHFGIQASDWRAALNKLNGYL

Sequences:

>Translated_291_residues
MKILVTGSNGQVGSCLVKQLSQMPEIEFWAVDRTQLDITDAAAVAKLVNDFKPHAIINAAAHTAVDKAEDEVALSYAINR
DGPQWLAEAANSAGAVMLHISTDYVFAGDKQGEYRETDAIDPQGVYGKSKLAGELAVAAACPRHIILRTAWVFCETGNNF
VKTMLRLSQSRDELGVVADQFGGPTYAGDIANALITMAKAISNGNQSFGIYHYSGLPHVNWHLFACEIFKQAKSQGLLDK
EMNVKAITSEQYPTPAKRPKNSKLDCNKILQHFGIQASDWRAALNKLNGYL
>Mature_291_residues
MKILVTGSNGQVGSCLVKQLSQMPEIEFWAVDRTQLDITDAAAVAKLVNDFKPHAIINAAAHTAVDKAEDEVALSYAINR
DGPQWLAEAANSAGAVMLHISTDYVFAGDKQGEYRETDAIDPQGVYGKSKLAGELAVAAACPRHIILRTAWVFCETGNNF
VKTMLRLSQSRDELGVVADQFGGPTYAGDIANALITMAKAISNGNQSFGIYHYSGLPHVNWHLFACEIFKQAKSQGLLDK
EMNVKAITSEQYPTPAKRPKNSKLDCNKILQHFGIQASDWRAALNKLNGYL

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=303, Percent_Identity=28.0528052805281, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI11034825, Length=303, Percent_Identity=28.0528052805281, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1788352, Length=289, Percent_Identity=39.7923875432526, Blast_Score=219, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 31699; Mature: 31699

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGSNGQVGSCLVKQLSQMPEIEFWAVDRTQLDITDAAAVAKLVNDFKPHAIINAA
CEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCEECHHHHHHHHHHHHCCCCCHHHHHH
AHTAVDKAEDEVALSYAINRDGPQWLAEAANSAGAVMLHISTDYVFAGDKQGEYRETDAI
HHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCCCEEEEEEECCEEEECCCCCCCCCCCCC
DPQGVYGKSKLAGELAVAAACPRHIILRTAWVFCETGNNFVKTMLRLSQSRDELGVVADQ
CCCCCCCCHHHHHHHHHHHHCCHHHHHHEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHH
FGGPTYAGDIANALITMAKAISNGNQSFGIYHYSGLPHVNWHLFACEIFKQAKSQGLLDK
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
EMNVKAITSEQYPTPAKRPKNSKLDCNKILQHFGIQASDWRAALNKLNGYL
CCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKILVTGSNGQVGSCLVKQLSQMPEIEFWAVDRTQLDITDAAAVAKLVNDFKPHAIINAA
CEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCEECHHHHHHHHHHHHCCCCCHHHHHH
AHTAVDKAEDEVALSYAINRDGPQWLAEAANSAGAVMLHISTDYVFAGDKQGEYRETDAI
HHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCCCEEEEEEECCEEEECCCCCCCCCCCCC
DPQGVYGKSKLAGELAVAAACPRHIILRTAWVFCETGNNFVKTMLRLSQSRDELGVVADQ
CCCCCCCCHHHHHHHHHHHHCCHHHHHHEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHH
FGGPTYAGDIANALITMAKAISNGNQSFGIYHYSGLPHVNWHLFACEIFKQAKSQGLLDK
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
EMNVKAITSEQYPTPAKRPKNSKLDCNKILQHFGIQASDWRAALNKLNGYL
CCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]