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The map label for this gene is pdhC [H]

Identifier: 126174436

GI number: 126174436

Start: 2568012

End: 2569637

Strand: Reverse

Name: pdhC [H]

Synonym: Sbal_2220

Alternate gene names: 126174436

Gene position: 2569637-2568012 (Counterclockwise)

Preceding gene: 126174437

Following gene: 126174435

Centisome position: 50.12

GC content: 50.06

Gene sequence:

>1626_bases
ATGATTAAAGATTTTATTTTGCCGGATATTGGCGAAGGTGTAGTGGAGTGTGAATTGGTTGAATGGTTGGTCAAAGAAGG
CGACACAGTCGTTGAAGATCAGCCGATTGCCGATGTCATGACAGACAAAGCCCTAGTGCAAATCCCTGCGCCATTTGCGG
GCGTCGTCACTAAGCTGTATTACGCCAAGGGCGATATTGCGAAAGTGCATGCGCCGCTCTATGCCGTACAAATTGAAGGT
GCCGTTGACATCGCGAGTGAAGAGTCTATTGCGACTGAACCTGCTGCGACGACTGCAAAAGTGACTGAGCCTGTGGCGGC
AACAACTGCCAATACGTCATCCAGTTCATCCGTCAGTATTGAAGAATTCTTACTACCGGATATCGGCGAAGGCATTGTTG
AATGTGAACTCGTTGAATGGTTAGTGAGTGAAGGTGATTGGGTTGAGGAAGATCAACCGATTGCCGACGTAATGACAGAT
AAAGCGCTGGTGCAAATTCCTGCCATTAAAGCGGGTAAAATTGCCAAACTGCATTATCGCAAAGGTCAGCTAGCTAAAGT
ACATGCGCCTTTATTTGCCATTGAAGTTGAGCATGCGGCATCTGTACCTGCGGCGACAACAAATACTGATACTGTGGTCA
ATGCGGCACCGGCAGCGCAGATTATGAGTGCAGAACCTGCTCGTCAAGGTAAAGCCTTGGCCAGCCCTGCGGTTCGTCGC
ATGGCGCGGTCGTTAGATATCGATTTGAGCCAAGTACCTGGCACAGGAAAGCACGGCCGTGTGTACAAGGAAGACATCAC
TCGTTTCCAACAAGGCGCGAGCAATGTTTCTGCTCCAAGTACGACTCAGGTAAAAGAGGCGCCAGCTCAAGCGACGCAAG
CATCTCAAACCCAAGTTCCAACATCGACAGTGACTCAACGCGCTGACACGGTTGAGCCTATTCGCGGCGTAAAAGCTGTG
ATGGCACGCATGATGGTCGAGTCGGTGTCGAGCATTCCGCACTTTACCTATTGCGAAGAGTTTGATTTAACAGACTTAGT
CGCACTGCGTGAAAGCATGAAAGTGAAGTATTCGTCTGATGAAGTGAAGCTGACCATGATGCCTTTCTTCATGAAGTCGA
TGTCGCTCGCGTTAAGTCAGTTCCCTGTGATGAATAGCCAAGTGAATGCTGATTGTACTGAGCTGACTTACAAGGCGCGC
CACAATATCTGCATGGCGGTGGATTCTAAAGTCGGTCTCTTGGTGCCCAACATCAAAGATGTGCAGGACAAGAGCATTTT
AGAAGTGGCGGCAGAAATCACCCGTCTGACCCAAGCAGCCCGCAGTGGCCGTGTGGCGCCAGCGGATCTGAAAGACGGCA
CGATTTCCATCTCTAACATTGGCGCCTTAGGTGGCACAGTGGCAACGCCTATTATCAACAAACCTGAAGTCGCGATTGTG
GCCTTAGGTAAGTTGCAAACCTTGCCGCGCTTTAATGCGAAAGGGGAAGTCGAAGCCCGCCAAATCATGCAAGTAAGCTG
GTCTGGTGACCACAGAGTGATCGATGGTGGCACCATTGCTCGCTTCTGTAATCTGTGGAAACAATATCTGGAGCAGCCGC
AAGAGATGTTATTGGCCATGCGCTAA

Upstream 100 bases:

>100_bases
AGATACCCCGTACCCACTCATTCATGAAAAAGAATACATGCCTGATGCGTTCAAAACGTTTGAAGCCATCAAAGCATCAG
TGACCTTCTAGGAGTCCGGT

Downstream 100 bases:

>100_bases
GCCGCTGGCACGCAGATTTGACAGTAAAAGGACGCTTTAGAGCGTCCTTTTTTATTTGCTTTGCACAAATGGGTTAATCT
TTTGATCCCGATTACGTATA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MIKDFILPDIGEGVVECELVEWLVKEGDTVVEDQPIADVMTDKALVQIPAPFAGVVTKLYYAKGDIAKVHAPLYAVQIEG
AVDIASEESIATEPAATTAKVTEPVAATTANTSSSSSVSIEEFLLPDIGEGIVECELVEWLVSEGDWVEEDQPIADVMTD
KALVQIPAIKAGKIAKLHYRKGQLAKVHAPLFAIEVEHAASVPAATTNTDTVVNAAPAAQIMSAEPARQGKALASPAVRR
MARSLDIDLSQVPGTGKHGRVYKEDITRFQQGASNVSAPSTTQVKEAPAQATQASQTQVPTSTVTQRADTVEPIRGVKAV
MARMMVESVSSIPHFTYCEEFDLTDLVALRESMKVKYSSDEVKLTMMPFFMKSMSLALSQFPVMNSQVNADCTELTYKAR
HNICMAVDSKVGLLVPNIKDVQDKSILEVAAEITRLTQAARSGRVAPADLKDGTISISNIGALGGTVATPIINKPEVAIV
ALGKLQTLPRFNAKGEVEARQIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPQEMLLAMR

Sequences:

>Translated_541_residues
MIKDFILPDIGEGVVECELVEWLVKEGDTVVEDQPIADVMTDKALVQIPAPFAGVVTKLYYAKGDIAKVHAPLYAVQIEG
AVDIASEESIATEPAATTAKVTEPVAATTANTSSSSSVSIEEFLLPDIGEGIVECELVEWLVSEGDWVEEDQPIADVMTD
KALVQIPAIKAGKIAKLHYRKGQLAKVHAPLFAIEVEHAASVPAATTNTDTVVNAAPAAQIMSAEPARQGKALASPAVRR
MARSLDIDLSQVPGTGKHGRVYKEDITRFQQGASNVSAPSTTQVKEAPAQATQASQTQVPTSTVTQRADTVEPIRGVKAV
MARMMVESVSSIPHFTYCEEFDLTDLVALRESMKVKYSSDEVKLTMMPFFMKSMSLALSQFPVMNSQVNADCTELTYKAR
HNICMAVDSKVGLLVPNIKDVQDKSILEVAAEITRLTQAARSGRVAPADLKDGTISISNIGALGGTVATPIINKPEVAIV
ALGKLQTLPRFNAKGEVEARQIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPQEMLLAMR
>Mature_541_residues
MIKDFILPDIGEGVVECELVEWLVKEGDTVVEDQPIADVMTDKALVQIPAPFAGVVTKLYYAKGDIAKVHAPLYAVQIEG
AVDIASEESIATEPAATTAKVTEPVAATTANTSSSSSVSIEEFLLPDIGEGIVECELVEWLVSEGDWVEEDQPIADVMTD
KALVQIPAIKAGKIAKLHYRKGQLAKVHAPLFAIEVEHAASVPAATTNTDTVVNAAPAAQIMSAEPARQGKALASPAVRR
MARSLDIDLSQVPGTGKHGRVYKEDITRFQQGASNVSAPSTTQVKEAPAQATQASQTQVPTSTVTQRADTVEPIRGVKAV
MARMMVESVSSIPHFTYCEEFDLTDLVALRESMKVKYSSDEVKLTMMPFFMKSMSLALSQFPVMNSQVNADCTELTYKAR
HNICMAVDSKVGLLVPNIKDVQDKSILEVAAEITRLTQAARSGRVAPADLKDGTISISNIGALGGTVATPIINKPEVAIV
ALGKLQTLPRFNAKGEVEARQIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPQEMLLAMR

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=423, Percent_Identity=43.9716312056738, Blast_Score=348, Evalue=1e-95,
Organism=Homo sapiens, GI31711992, Length=571, Percent_Identity=24.6935201401051, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI203098816, Length=449, Percent_Identity=26.9487750556793, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI203098753, Length=449, Percent_Identity=26.5033407572383, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI19923748, Length=235, Percent_Identity=30.2127659574468, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI260898739, Length=136, Percent_Identity=34.5588235294118, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1786305, Length=554, Percent_Identity=30.6859205776173, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1786946, Length=424, Percent_Identity=26.1792452830189, Blast_Score=155, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17537937, Length=438, Percent_Identity=39.2694063926941, Blast_Score=317, Evalue=9e-87,
Organism=Caenorhabditis elegans, GI25146366, Length=461, Percent_Identity=27.765726681128, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=27.6643990929705, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=321, Percent_Identity=24.9221183800623, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320352, Length=443, Percent_Identity=25.2821670428894, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324258, Length=446, Percent_Identity=27.1300448430493, Blast_Score=137, Evalue=5e-33,
Organism=Drosophila melanogaster, GI18859875, Length=430, Percent_Identity=41.1627906976744, Blast_Score=318, Evalue=5e-87,
Organism=Drosophila melanogaster, GI20129315, Length=451, Percent_Identity=24.390243902439, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24582497, Length=313, Percent_Identity=26.517571884984, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24645909, Length=198, Percent_Identity=30.8080808080808, Blast_Score=99, Evalue=1e-20,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 58311; Mature: 58311

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKDFILPDIGEGVVECELVEWLVKEGDTVVEDQPIADVMTDKALVQIPAPFAGVVTKLY
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHCCCCEEEECCCHHHHHHHHH
YAKGDIAKVHAPLYAVQIEGAVDIASEESIATEPAATTAKVTEPVAATTANTSSSSSVSI
HCCCCCEEEECCEEEEEECCCEEECCCCCCCCCCCCCCHHHCCCCCEEECCCCCCCCCCH
EEFLLPDIGEGIVECELVEWLVSEGDWVEEDQPIADVMTDKALVQIPAIKAGKIAKLHYR
HHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCEEEEEEC
KGQLAKVHAPLFAIEVEHAASVPAATTNTDTVVNAAPAAQIMSAEPARQGKALASPAVRR
CCCCEEEECCEEEEEEHHHCCCCCCCCCCCCEEECCCHHHHHCCCCHHCCCHHHHHHHHH
MARSLDIDLSQVPGTGKHGRVYKEDITRFQQGASNVSAPSTTQVKEAPAQATQASQTQVP
HHHHCCCCHHHCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHCHHCCCC
TSTVTQRADTVEPIRGVKAVMARMMVESVSSIPHFTYCEEFDLTDLVALRESMKVKYSSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCEEECCC
EVKLTMMPFFMKSMSLALSQFPVMNSQVNADCTELTYKARHNICMAVDSKVGLLVPNIKD
CEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCEECCCCCC
VQDKSILEVAAEITRLTQAARSGRVAPADLKDGTISISNIGALGGTVATPIINKPEVAIV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHCCCCCCCCEEEE
ALGKLQTLPRFNAKGEVEARQIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPQEMLLAM
EECHHHCCCCCCCCCCCCHHHHEEEEECCCEEEECCHHHHHHHHHHHHHHHCHHHHHHHC
R
C
>Mature Secondary Structure
MIKDFILPDIGEGVVECELVEWLVKEGDTVVEDQPIADVMTDKALVQIPAPFAGVVTKLY
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHCCCCEEEECCCHHHHHHHHH
YAKGDIAKVHAPLYAVQIEGAVDIASEESIATEPAATTAKVTEPVAATTANTSSSSSVSI
HCCCCCEEEECCEEEEEECCCEEECCCCCCCCCCCCCCHHHCCCCCEEECCCCCCCCCCH
EEFLLPDIGEGIVECELVEWLVSEGDWVEEDQPIADVMTDKALVQIPAIKAGKIAKLHYR
HHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCEEEEEEC
KGQLAKVHAPLFAIEVEHAASVPAATTNTDTVVNAAPAAQIMSAEPARQGKALASPAVRR
CCCCEEEECCEEEEEEHHHCCCCCCCCCCCCEEECCCHHHHHCCCCHHCCCHHHHHHHHH
MARSLDIDLSQVPGTGKHGRVYKEDITRFQQGASNVSAPSTTQVKEAPAQATQASQTQVP
HHHHCCCCHHHCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHCHHCCCC
TSTVTQRADTVEPIRGVKAVMARMMVESVSSIPHFTYCEEFDLTDLVALRESMKVKYSSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCEEECCC
EVKLTMMPFFMKSMSLALSQFPVMNSQVNADCTELTYKARHNICMAVDSKVGLLVPNIKD
CEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCEECCCCCC
VQDKSILEVAAEITRLTQAARSGRVAPADLKDGTISISNIGALGGTVATPIINKPEVAIV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHCCCCCCCCEEEE
ALGKLQTLPRFNAKGEVEARQIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPQEMLLAM
EECHHHCCCCCCCCCCCCHHHHEEEEECCCEEEECCHHHHHHHHHHHHHHHCHHHHHHHC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]