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The map label for this gene is napA [H]

Identifier: 126174136

GI number: 126174136

Start: 2219344

End: 2221833

Strand: Direct

Name: napA [H]

Synonym: Sbal_1910

Alternate gene names: 126174136

Gene position: 2219344-2221833 (Clockwise)

Preceding gene: 126174135

Following gene: 126174137

Centisome position: 43.28

GC content: 50.6

Gene sequence:

>2490_bases
ATGAACAGACGTGATTTTATTAAAGCCAATGCGGTGATTGCTGCCGCAGGGGCCGCAGGATTAGCTTTGCCCGCAGGCGC
AAGCAACCTTATTACCAGCTCAGAACAAACCAAATTAGAGTGGAACAAGGCGCCGTGCCGCTTCTGTGGCACTGGCTGCT
CTGTGATTGTCGCGACACGCGACGGCAAAGTGGTCGCCACCCATGGCGATGCTAACAGTGAAGTGAATCGTGGCCTTAAC
TGTATTAAAGGCTATTTTCTGTCGAAGATTATGTACGGCAGCGATCGCTTAACCACGCCCATGCTGCGGATGACGGATGG
CAAGTACGACAAACACGGTGAGTTTTCGCCAATTAGCTGGGACAGCGCCTTCGACATCATGGCACAAAAGTGGAAAGAAA
CCATTAAAGCCAAAGGGCCAACGGCCGTGGGCATGTTTGGTTCGGGCCAATGGACTGTGTGGGAAGGTTACGCCGCCGTT
AAGTTGATGAAGGCGGGCTTTGGTTCGAACAATATCGACCCTAATGCGCGCCATTGTATGGCTTCAGCCGTGGTCGGATT
TATGCGCACTTTCGGTATCGACGAGCCTATGGGCTGCTATGACGATATGGAAGCCGCCGATGCATTCGTGCTTTGGGGCT
CGAACATGGCCGAGATGCACCCGATCCTGTGGAGCCGAGTAACAGACAGACGCTTGAGTGAACCCCATGTCAAAGTAGCT
GTGTTATCGACTTTCCAGAATCGTTCCTTCGATCTGGCGGATATTCCGATAGTGTTCACCCCGCAAACGGATTTGGCCAT
GTTGAATTTTATTGCCAACTACATTATTCAAAACGATAAAGTGAACTGGGATTTCGTTAAAAAGCACGTGAATTTCCGCC
AAGGCACGACTGATATCGGCTACGGTTTACGTCCGACTCATCCCCTTGAGCAAAAAGCTAAAAATGTGAAAACCGCAGGG
GATTCAACGGCAATTGATTTTGAAGCCTTCAAAGCCTTTGTTGCCGAATATACAGTCGAGAAAGTCAGTAAACTGTCTGG
GGTGCCGGAAGATAAACTGATCGAATTGGCTAAGTTATATGCCGATCCGAATGTCAAAGTGACGTCGTTCTGGACCATGG
GATTTAACCAACATACCCGTGGTGTGTGGTGTAATAACCTGATTTACAACATCCATTTGTTAACAGGTAAGATTTCAACC
CCAGGTAACAGCCCATTTTCGCTGACCGGTCAGCCATCGGCCTGTGGCACGGCACGTGAAGTCGGGACTTTCTCCCATCG
TCTACCCGCCGACATGGTGGTGACCAATCCCGAGCACAGAAAACGTGCCGAAGAAACGTGGAAAATTCCAAGCGGCATCA
TTCCGCCCAAACCCGGTTATCACGCCGTTGAGCAAAACCGCCGCCTTAAAGACGGTGACTTGAACTGCTATTGGGTACAA
GTGAATAACAATATGCAGGCGGGTCCGAATATCAACGAAGAAGGGTTGCCGGGCTATCGTAATCCCGAAAACTTTATCGT
GGTGTCCGATGCTTATCCCACCGTCACCACTCAAGCTGCCGATCTTATTTTGCCCACTGCAATGTGGGTCGAGAAAGAGG
GCGCTTACGGTAACGCCGAGCGCCGTACTCAGTTCTGGCATCAAATGGTGCAAGCGCCGGGCGAATCGCATTCGGATCTG
TGGCAGTTGATGGAATTTGCGAAACGTTTCACCACAGATGAAGTGTGGCCCAAGGATGTATTAGCCGCGAATCCAGAGTT
CAAAGGCAAAACGCTTTTCGAAGTCCTGTATCAAAATGGCCATGTCGATAAGTTTCCGTTGGCGGATGCCGATCCTAAGT
ATCTGAACGATGAAGCGAAACATTTTGGCTTCTATGTGCAAAAAGGTCTGTTTGAAGAGTACGCCTCCTTTGGCCGTGGT
CATGGTCATGACTTAGCGCCGTTTGATGCCTATCACGAGGCCCATGGTCTGCGTTGGCCTGTGGTCGATGGCAAGGAAAC
CAAGTGGCGTTTCCGTGAGGGGAGCGATCCTTATGTGAAAGCGGGGACAGGCTTTGAGTTTTATGGTCATCCCGATGGCC
GTGCGGTGATTTTTGCGCTGCCCTATGAGCCCGCCGCCGAGTCGCCAGACGAAGAATACGATATCTGGTTATCGACGGGC
CGAGTGCTGGAACATTGGCACTCAGGTTCTATGACCCAACGTGTGCCTGAGCTTTATCGCGCTTTCCCTGATGCCGTGTG
TTTTATGCATCCAGAAGATGCCAAGAAGCGTGGTCTGCGCCGTGGCGATGAAATTCGTGTGGTGTCACGCCGTGGTGAAA
TTAAGACCCGAGTTGAAACCCGTGGCCGTAACAAACCGCCTGTGGGCTTAGTGTTTGTGCCTTGGTTCGATGCTAGCCAG
CTCATTAATAAAGTCACTTTAGATGCGACAGATCCCTTGTCAAAACAAACTGACTTTAAAAAATGTGCGGTGAAGATCGT
CAAGGTTTAA

Upstream 100 bases:

>100_bases
TGATGGACAGTATAGAAGCGATCAATGCCATTCCGGGCGTGTTATCCGCCACTATGGTTTACCACCAGAGTGAAGTGCTC
GAAGAGGGTGAAGAATGAAT

Downstream 100 bases:

>100_bases
GGAGAACACGATGAAAACTCTACAATTAGTTTCAGCGATCGGTTTACTGGCATTCGGTTCATTGATGATGACGGCAGTCT
CTGCCGCCAGTATTCCCGAC

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 829; Mature: 829

Protein sequence:

>829_residues
MNRRDFIKANAVIAAAGAAGLALPAGASNLITSSEQTKLEWNKAPCRFCGTGCSVIVATRDGKVVATHGDANSEVNRGLN
CIKGYFLSKIMYGSDRLTTPMLRMTDGKYDKHGEFSPISWDSAFDIMAQKWKETIKAKGPTAVGMFGSGQWTVWEGYAAV
KLMKAGFGSNNIDPNARHCMASAVVGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSEPHVKVA
VLSTFQNRSFDLADIPIVFTPQTDLAMLNFIANYIIQNDKVNWDFVKKHVNFRQGTTDIGYGLRPTHPLEQKAKNVKTAG
DSTAIDFEAFKAFVAEYTVEKVSKLSGVPEDKLIELAKLYADPNVKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKIST
PGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPEHRKRAEETWKIPSGIIPPKPGYHAVEQNRRLKDGDLNCYWVQ
VNNNMQAGPNINEEGLPGYRNPENFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAERRTQFWHQMVQAPGESHSDL
WQLMEFAKRFTTDEVWPKDVLAANPEFKGKTLFEVLYQNGHVDKFPLADADPKYLNDEAKHFGFYVQKGLFEEYASFGRG
HGHDLAPFDAYHEAHGLRWPVVDGKETKWRFREGSDPYVKAGTGFEFYGHPDGRAVIFALPYEPAAESPDEEYDIWLSTG
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPEDAKKRGLRRGDEIRVVSRRGEIKTRVETRGRNKPPVGLVFVPWFDASQ
LINKVTLDATDPLSKQTDFKKCAVKIVKV

Sequences:

>Translated_829_residues
MNRRDFIKANAVIAAAGAAGLALPAGASNLITSSEQTKLEWNKAPCRFCGTGCSVIVATRDGKVVATHGDANSEVNRGLN
CIKGYFLSKIMYGSDRLTTPMLRMTDGKYDKHGEFSPISWDSAFDIMAQKWKETIKAKGPTAVGMFGSGQWTVWEGYAAV
KLMKAGFGSNNIDPNARHCMASAVVGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSEPHVKVA
VLSTFQNRSFDLADIPIVFTPQTDLAMLNFIANYIIQNDKVNWDFVKKHVNFRQGTTDIGYGLRPTHPLEQKAKNVKTAG
DSTAIDFEAFKAFVAEYTVEKVSKLSGVPEDKLIELAKLYADPNVKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKIST
PGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPEHRKRAEETWKIPSGIIPPKPGYHAVEQNRRLKDGDLNCYWVQ
VNNNMQAGPNINEEGLPGYRNPENFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAERRTQFWHQMVQAPGESHSDL
WQLMEFAKRFTTDEVWPKDVLAANPEFKGKTLFEVLYQNGHVDKFPLADADPKYLNDEAKHFGFYVQKGLFEEYASFGRG
HGHDLAPFDAYHEAHGLRWPVVDGKETKWRFREGSDPYVKAGTGFEFYGHPDGRAVIFALPYEPAAESPDEEYDIWLSTG
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPEDAKKRGLRRGDEIRVVSRRGEIKTRVETRGRNKPPVGLVFVPWFDASQ
LINKVTLDATDPLSKQTDFKKCAVKIVKV
>Mature_829_residues
MNRRDFIKANAVIAAAGAAGLALPAGASNLITSSEQTKLEWNKAPCRFCGTGCSVIVATRDGKVVATHGDANSEVNRGLN
CIKGYFLSKIMYGSDRLTTPMLRMTDGKYDKHGEFSPISWDSAFDIMAQKWKETIKAKGPTAVGMFGSGQWTVWEGYAAV
KLMKAGFGSNNIDPNARHCMASAVVGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSEPHVKVA
VLSTFQNRSFDLADIPIVFTPQTDLAMLNFIANYIIQNDKVNWDFVKKHVNFRQGTTDIGYGLRPTHPLEQKAKNVKTAG
DSTAIDFEAFKAFVAEYTVEKVSKLSGVPEDKLIELAKLYADPNVKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKIST
PGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPEHRKRAEETWKIPSGIIPPKPGYHAVEQNRRLKDGDLNCYWVQ
VNNNMQAGPNINEEGLPGYRNPENFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAERRTQFWHQMVQAPGESHSDL
WQLMEFAKRFTTDEVWPKDVLAANPEFKGKTLFEVLYQNGHVDKFPLADADPKYLNDEAKHFGFYVQKGLFEEYASFGRG
HGHDLAPFDAYHEAHGLRWPVVDGKETKWRFREGSDPYVKAGTGFEFYGHPDGRAVIFALPYEPAAESPDEEYDIWLSTG
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPEDAKKRGLRRGDEIRVVSRRGEIKTRVETRGRNKPPVGLVFVPWFDASQ
LINKVTLDATDPLSKQTDFKKCAVKIVKV

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=829, Percent_Identity=71.773220747889, Blast_Score=1276, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=794, Percent_Identity=23.8035264483627, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI3868720, Length=666, Percent_Identity=23.1231231231231, Blast_Score=116, Evalue=7e-27,
Organism=Escherichia coli, GI1787870, Length=883, Percent_Identity=23.4428086070215, Blast_Score=109, Evalue=9e-25,
Organism=Escherichia coli, GI3868719, Length=480, Percent_Identity=23.5416666666667, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87081797, Length=903, Percent_Identity=22.1483942414175, Blast_Score=93, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 92872; Mature: 92872

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRDFIKANAVIAAAGAAGLALPAGASNLITSSEQTKLEWNKAPCRFCGTGCSVIVATR
CCCCCEEEECEEEEECCCCCEECCCCCCHHCCCCCCCEEECCCCCCCEECCCCEEEEEEC
DGKVVATHGDANSEVNRGLNCIKGYFLSKIMYGSDRLTTPMLRMTDGKYDKHGEFSPISW
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCC
DSAFDIMAQKWKETIKAKGPTAVGMFGSGQWTVWEGYAAVKLMKAGFGSNNIDPNARHCM
CHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHHHH
ASAVVGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSEPHVKVA
HHHHHHHHHHHCCCCCCCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEE
VLSTFQNRSFDLADIPIVFTPQTDLAMLNFIANYIIQNDKVNWDFVKKHVNFRQGTTDIG
EEEHHCCCCCCEECCCEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCC
YGLRPTHPLEQKAKNVKTAGDSTAIDFEAFKAFVAEYTVEKVSKLSGVPEDKLIELAKLY
CCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ADPNVKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKISTPGNSPFSLTGQPSACGTARE
CCCCEEEEEEEEECCCCCCCCEEECCEEEEEEEEEEEECCCCCCCEEECCCCCCCCCHHH
VGTFSHRLPADMVVTNPEHRKRAEETWKIPSGIIPPKPGYHAVEQNRRLKDGDLNCYWVQ
HCCHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEEEE
VNNNMQAGPNINEEGLPGYRNPENFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAE
ECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHEEECCCCCCCHH
RRTQFWHQMVQAPGESHSDLWQLMEFAKRFTTDEVWPKDVLAANPEFKGKTLFEVLYQNG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHHCC
HVDKFPLADADPKYLNDEAKHFGFYVQKGLFEEYASFGRGHGHDLAPFDAYHEAHGLRWP
CCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCC
VVDGKETKWRFREGSDPYVKAGTGFEFYGHPDGRAVIFALPYEPAAESPDEEYDIWLSTG
CCCCCCCCEEECCCCCCEEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEECC
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPEDAKKRGLRRGDEIRVVSRRGEIKTRVET
HHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCCEEEEECCCCEEEHHHH
RGRNKPPVGLVFVPWFDASQLINKVTLDATDPLSKQTDFKKCAVKIVKV
CCCCCCCEEEEEECCCCHHHHHHHEECCCCCCCCCCCHHHHHHHEEECC
>Mature Secondary Structure
MNRRDFIKANAVIAAAGAAGLALPAGASNLITSSEQTKLEWNKAPCRFCGTGCSVIVATR
CCCCCEEEECEEEEECCCCCEECCCCCCHHCCCCCCCEEECCCCCCCEECCCCEEEEEEC
DGKVVATHGDANSEVNRGLNCIKGYFLSKIMYGSDRLTTPMLRMTDGKYDKHGEFSPISW
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCC
DSAFDIMAQKWKETIKAKGPTAVGMFGSGQWTVWEGYAAVKLMKAGFGSNNIDPNARHCM
CHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHHHH
ASAVVGFMRTFGIDEPMGCYDDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSEPHVKVA
HHHHHHHHHHHCCCCCCCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEE
VLSTFQNRSFDLADIPIVFTPQTDLAMLNFIANYIIQNDKVNWDFVKKHVNFRQGTTDIG
EEEHHCCCCCCEECCCEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCC
YGLRPTHPLEQKAKNVKTAGDSTAIDFEAFKAFVAEYTVEKVSKLSGVPEDKLIELAKLY
CCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ADPNVKVTSFWTMGFNQHTRGVWCNNLIYNIHLLTGKISTPGNSPFSLTGQPSACGTARE
CCCCEEEEEEEEECCCCCCCCEEECCEEEEEEEEEEEECCCCCCCEEECCCCCCCCCHHH
VGTFSHRLPADMVVTNPEHRKRAEETWKIPSGIIPPKPGYHAVEQNRRLKDGDLNCYWVQ
HCCHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEEEE
VNNNMQAGPNINEEGLPGYRNPENFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAE
ECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHEEECCCCCCCHH
RRTQFWHQMVQAPGESHSDLWQLMEFAKRFTTDEVWPKDVLAANPEFKGKTLFEVLYQNG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHHCC
HVDKFPLADADPKYLNDEAKHFGFYVQKGLFEEYASFGRGHGHDLAPFDAYHEAHGLRWP
CCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCC
VVDGKETKWRFREGSDPYVKAGTGFEFYGHPDGRAVIFALPYEPAAESPDEEYDIWLSTG
CCCCCCCCEEECCCCCCEEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEECC
RVLEHWHSGSMTQRVPELYRAFPDAVCFMHPEDAKKRGLRRGDEIRVVSRRGEIKTRVET
HHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCCEEEEECCCCEEEHHHH
RGRNKPPVGLVFVPWFDASQLINKVTLDATDPLSKQTDFKKCAVKIVKV
CCCCCCCEEEEEECCCCHHHHHHHEECCCCCCCCCCCHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA