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The map label for this gene is napB [H]

Identifier: 126174137

GI number: 126174137

Start: 2221844

End: 2222329

Strand: Direct

Name: napB [H]

Synonym: Sbal_1911

Alternate gene names: 126174137

Gene position: 2221844-2222329 (Clockwise)

Preceding gene: 126174136

Following gene: 126174138

Centisome position: 43.33

GC content: 49.59

Gene sequence:

>486_bases
ATGAAAACTCTACAATTAGTTTCAGCGATCGGTTTACTGGCATTCGGTTCATTGATGATGACGGCAGTCTCTGCCGCCAG
TATTCCCGACGAAAAAATAGCGACCATGCGACAAGCGCCAATTGATGTGGAACCGACGCCGCCAGCGATGCAAAACGTAC
GTAATACCGATGTGAAGGAAGTGCGCAGCTACCCCATGCAGCCGCCTGTGATTCCGCACAAGATCGACGGTTATCAGCTC
GATCTTAAAGTGAACAAGTGTATGGCTTGCCATGCTCGTAGCCGCATTGGGGATTCGCAAGCGCCTATGGTCAGCGTCAC
GCATTTTATGGACAGGGACAATAACTTCTTAGCCGAGTTATCGCCGCGCCGCTATTTCTGCACTCAGTGTCATGTGCCGC
AGTTAGATGCCAAGTTACTGGTCGAAAACGACTTTGTGGATATGGAACATCTGATCAAAGCCAACACTGAGCAGAAAAAA
CACTAG

Upstream 100 bases:

>100_bases
CTCATTAATAAAGTCACTTTAGATGCGACAGATCCCTTGTCAAAACAAACTGACTTTAAAAAATGTGCGGTGAAGATCGT
CAAGGTTTAAGGAGAACACG

Downstream 100 bases:

>100_bases
GAGTTAGCATGTTAGATAAACTGAAAAAAATCTGGCAGGTACTCAATCGTCCGAGTGTGCATTACAGTTTAGGTTTTTTG
ACCTTAGGTGGCTTTGTGGC

Product: nitrate reductase cytochrome c-type subunit (NapB)

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MKTLQLVSAIGLLAFGSLMMTAVSAASIPDEKIATMRQAPIDVEPTPPAMQNVRNTDVKEVRSYPMQPPVIPHKIDGYQL
DLKVNKCMACHARSRIGDSQAPMVSVTHFMDRDNNFLAELSPRRYFCTQCHVPQLDAKLLVENDFVDMEHLIKANTEQKK
H

Sequences:

>Translated_161_residues
MKTLQLVSAIGLLAFGSLMMTAVSAASIPDEKIATMRQAPIDVEPTPPAMQNVRNTDVKEVRSYPMQPPVIPHKIDGYQL
DLKVNKCMACHARSRIGDSQAPMVSVTHFMDRDNNFLAELSPRRYFCTQCHVPQLDAKLLVENDFVDMEHLIKANTEQKK
H
>Mature_161_residues
MKTLQLVSAIGLLAFGSLMMTAVSAASIPDEKIATMRQAPIDVEPTPPAMQNVRNTDVKEVRSYPMQPPVIPHKIDGYQL
DLKVNKCMACHARSRIGDSQAPMVSVTHFMDRDNNFLAELSPRRYFCTQCHVPQLDAKLLVENDFVDMEHLIKANTEQKK
H

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=84, Percent_Identity=48.8095238095238, Blast_Score=100, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 18090; Mature: 18090

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLQLVSAIGLLAFGSLMMTAVSAASIPDEKIATMRQAPIDVEPTPPAMQNVRNTDVKE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHH
VRSYPMQPPVIPHKIDGYQLDLKVNKCMACHARSRIGDSQAPMVSVTHFMDRDNNFLAEL
HHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCEEHHHHHHCCCCCEEEEC
SPRRYFCTQCHVPQLDAKLLVENDFVDMEHLIKANTEQKKH
CCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKTLQLVSAIGLLAFGSLMMTAVSAASIPDEKIATMRQAPIDVEPTPPAMQNVRNTDVKE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHH
VRSYPMQPPVIPHKIDGYQLDLKVNKCMACHARSRIGDSQAPMVSVTHFMDRDNNFLAEL
HHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCEEHHHHHHCCCCCEEEEC
SPRRYFCTQCHVPQLDAKLLVENDFVDMEHLIKANTEQKKH
CCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376334; 12948488 [H]