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The map label for this gene is pheT [H]

Identifier: 126174100

GI number: 126174100

Start: 2173429

End: 2175816

Strand: Direct

Name: pheT [H]

Synonym: Sbal_1874

Alternate gene names: 126174100

Gene position: 2173429-2175816 (Clockwise)

Preceding gene: 126174099

Following gene: 126174101

Centisome position: 42.39

GC content: 48.07

Gene sequence:

>2388_bases
ATGAAATTTAGCGAATCTTGGCTTCGTGAATGGGTAAACCCTTCAGTCAGCCGTGAAGCATTATCGCACCAGATCACTAT
GGCCGGTCTCGAAGTCGATGACGTTGAAGCCGTAGCAGCCGATTTTAGCGGTGTCGTGATTGGTGAAGTGGTTGAGTGTG
GTCAGCATCCCGATGCTGATAAACTGCGCGTGACCAAAATCAACGTTGGTGGTAGCGAACTTATCGATATCGTCTGCGGT
GCACCAAACTGCCGTCAGGGCCTTAAAGTGGCCGTGGCTATGGTTGGTGCTGTTTTACCTGGCGATTTCAAAATCAAGAA
AGCTAAGCTGCGTGGCATGCCATCAGAAGGCATGTTGTGCTCGTACAGCGAGCTTGGCATTAACGTCGAAAGCGATGGCA
TTATCGAATTGCCATTGGACGCGCCACTTGGCACTGACATCCGCGATTATCTGCAACTTAATGATGCCGTTATCGATGTG
GATTTAACGGCTAACCGCGCCGATTGCTTAGGCATGGCAGGCCTTGCCCGTGAAGTGGGCGTGTTAAACCGCCAAGCCGT
GGTTGAGCCACAGTGGCAAGCCGTCACGGCAACGACAGATGCACAAGTCACGATTAACGTTAAGGCGCCAGACGCTTGTC
CACGTTACTTAGGCCGTGTGGTTAAAAACGTCAATGTTAAAGCGCCTACGCCATTATGGATGCAGGAAAAACTGCGCCGT
AGCGGTATTCGCTCTATCGATCCTATCGTTGATATCACTAACTTTGTGTTGATTGAATTCGGTCAACCTATGCACGCTTT
CGATCTTGCCAAGTTAACGGGCGATATCCAAGTGCGTTTAGGCACTGGCGAAGAGAAAATCACCCTGCTTGATGGCAGCG
AAGTGACCATCCCAAGCGATACCTTAGTGATCGCCGATGATGCGCGTCCATTAGCCCTTGCAGGCGTGTTTGGCGGCGAA
TATTCAGGCGTGAGTGACACGACCCAAGACATACTGCTTGAATGTGCGTTCTTCGCGCCTCTTGCCATTATGGGTAAGTC
ACGTCGTTTAGGCTTGCACACAGATTCATCACACCGCTTTGAGCGTGGCGTTGACCCTGAGATGCAGCACAAGGTTATCG
ACCGTGCGACCCTTTTAGTGCTGGATATCTGTGGCGGTGAAGCGGGCCCTGTAGTTGAAGCTAAATCAGAGGCTGATTTA
CCTAAACCTGCACAAATCGTGTTACGTCGCAGCAAGTTAGATAGAATTTTAGGTCACCATGTGCCAGACACAGATGTGGT
CGAAATTCTACAACGTCTCGGCTTCAGTGTGGTTGTGGGCCAAGATAGCTGGCAGGTCACCACTGCGACTTATCGTTTCG
ATATGGCGATTGAAGAAGATTTGATTGAAGAAGTGGCGCGTATTTACGGTTATAACAATATCCCGAATATCGCACCCGTT
GCCGCGCTGCGCATGTCAGATCATAAAGAATCTGACTTAAGCTTAAAACGCGTGCGTGCCTTATTAGTCGCTCGTGGCTT
CCAAGAAGCGGTAACCTATAGCTTTGTCGATCCAAAACTGCAAAATCTGGTTCACCCTGGTTTAGACGCTATGGTGTTAC
CAAACCCAATTTCCAGTGAAATGTCGGCCATGCGCCTGTCAATGTTCACCGGTTTATTGGCCGCCGTTGGCTACAACCAA
AGTCGTCAACAGAGTCGCGTGAGACTTTTTGAAACTGGCCTGCGCTTTGTTCCTGATATCAATGCAGAATCCGGTGTAAG
ACAGCAAGCAATGCTCGCTTGTGTCATTACTGGGCTCCAAAATGATGAACATTGGGCTATGGAATCGAAAACTGTTGATT
TTTTCGACCTTAAAGGTGATTTAGAAGCAATTATCGGCTTGACAGTTTCCGCATCAGAATTTAGCTTTAGAGGAGCGACG
CATTCTGCTCTCCATCCGGGGCAATGTGCTGAAATATTGAGAAATGATCGAGTGATCGGTCATATAGGTGCAATCCATCC
TAGTTTGGAGAAGCCTTTTGGACTCAATGGTAAAACCATCGTTTTTGAGTTGGAATTGGATGCTTTATTGCATACCAGTT
TGCCGCTAGCCCAAGCTGTATCTAAGTTTCCAGCTAATCGTCGTGACATCGCAGTCGTAGTAGATGAGAGTGTATCTGCA
GGTGATGTAATGAAATTGATAAGAAAAGTTGGCGAAAATCAGTTGGTTGGCATAAACTTGTTCGATGTATACTTAGGTAA
AGGTGTTGAACCTGGCAAGAAGAGCCTGGCGATAGCACTTACATTACAAGACACTACTCGTACGCTTGAGGAAAAAGAGA
TTGCTGAGACCGTTGGTTCAGTCGTTTCTGCCCTTAAGTCCGAGTTCAACGCATCGTTGAGGGATTAA

Upstream 100 bases:

>100_bases
GCGCTTAACTATGTTGCGTTACGGCGTAAACGATTTACGTGCGTTTTTCGAAAACGACCTACGTTTCCTGAAGCAATTTA
AATAACGGAGCTGTAATAGC

Downstream 100 bases:

>100_bases
AGTATGGCACTTACCAAAGCCGAAATGGCAGAACATCTTTTTGAAACACTCGGAATGAACAAACGTGTCGCCAAAGAGAT
GGTTGAGTCGTTCTTTGAAG

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 795; Mature: 795

Protein sequence:

>795_residues
MKFSESWLREWVNPSVSREALSHQITMAGLEVDDVEAVAADFSGVVIGEVVECGQHPDADKLRVTKINVGGSELIDIVCG
APNCRQGLKVAVAMVGAVLPGDFKIKKAKLRGMPSEGMLCSYSELGINVESDGIIELPLDAPLGTDIRDYLQLNDAVIDV
DLTANRADCLGMAGLAREVGVLNRQAVVEPQWQAVTATTDAQVTINVKAPDACPRYLGRVVKNVNVKAPTPLWMQEKLRR
SGIRSIDPIVDITNFVLIEFGQPMHAFDLAKLTGDIQVRLGTGEEKITLLDGSEVTIPSDTLVIADDARPLALAGVFGGE
YSGVSDTTQDILLECAFFAPLAIMGKSRRLGLHTDSSHRFERGVDPEMQHKVIDRATLLVLDICGGEAGPVVEAKSEADL
PKPAQIVLRRSKLDRILGHHVPDTDVVEILQRLGFSVVVGQDSWQVTTATYRFDMAIEEDLIEEVARIYGYNNIPNIAPV
AALRMSDHKESDLSLKRVRALLVARGFQEAVTYSFVDPKLQNLVHPGLDAMVLPNPISSEMSAMRLSMFTGLLAAVGYNQ
SRQQSRVRLFETGLRFVPDINAESGVRQQAMLACVITGLQNDEHWAMESKTVDFFDLKGDLEAIIGLTVSASEFSFRGAT
HSALHPGQCAEILRNDRVIGHIGAIHPSLEKPFGLNGKTIVFELELDALLHTSLPLAQAVSKFPANRRDIAVVVDESVSA
GDVMKLIRKVGENQLVGINLFDVYLGKGVEPGKKSLAIALTLQDTTRTLEEKEIAETVGSVVSALKSEFNASLRD

Sequences:

>Translated_795_residues
MKFSESWLREWVNPSVSREALSHQITMAGLEVDDVEAVAADFSGVVIGEVVECGQHPDADKLRVTKINVGGSELIDIVCG
APNCRQGLKVAVAMVGAVLPGDFKIKKAKLRGMPSEGMLCSYSELGINVESDGIIELPLDAPLGTDIRDYLQLNDAVIDV
DLTANRADCLGMAGLAREVGVLNRQAVVEPQWQAVTATTDAQVTINVKAPDACPRYLGRVVKNVNVKAPTPLWMQEKLRR
SGIRSIDPIVDITNFVLIEFGQPMHAFDLAKLTGDIQVRLGTGEEKITLLDGSEVTIPSDTLVIADDARPLALAGVFGGE
YSGVSDTTQDILLECAFFAPLAIMGKSRRLGLHTDSSHRFERGVDPEMQHKVIDRATLLVLDICGGEAGPVVEAKSEADL
PKPAQIVLRRSKLDRILGHHVPDTDVVEILQRLGFSVVVGQDSWQVTTATYRFDMAIEEDLIEEVARIYGYNNIPNIAPV
AALRMSDHKESDLSLKRVRALLVARGFQEAVTYSFVDPKLQNLVHPGLDAMVLPNPISSEMSAMRLSMFTGLLAAVGYNQ
SRQQSRVRLFETGLRFVPDINAESGVRQQAMLACVITGLQNDEHWAMESKTVDFFDLKGDLEAIIGLTVSASEFSFRGAT
HSALHPGQCAEILRNDRVIGHIGAIHPSLEKPFGLNGKTIVFELELDALLHTSLPLAQAVSKFPANRRDIAVVVDESVSA
GDVMKLIRKVGENQLVGINLFDVYLGKGVEPGKKSLAIALTLQDTTRTLEEKEIAETVGSVVSALKSEFNASLRD
>Mature_795_residues
MKFSESWLREWVNPSVSREALSHQITMAGLEVDDVEAVAADFSGVVIGEVVECGQHPDADKLRVTKINVGGSELIDIVCG
APNCRQGLKVAVAMVGAVLPGDFKIKKAKLRGMPSEGMLCSYSELGINVESDGIIELPLDAPLGTDIRDYLQLNDAVIDV
DLTANRADCLGMAGLAREVGVLNRQAVVEPQWQAVTATTDAQVTINVKAPDACPRYLGRVVKNVNVKAPTPLWMQEKLRR
SGIRSIDPIVDITNFVLIEFGQPMHAFDLAKLTGDIQVRLGTGEEKITLLDGSEVTIPSDTLVIADDARPLALAGVFGGE
YSGVSDTTQDILLECAFFAPLAIMGKSRRLGLHTDSSHRFERGVDPEMQHKVIDRATLLVLDICGGEAGPVVEAKSEADL
PKPAQIVLRRSKLDRILGHHVPDTDVVEILQRLGFSVVVGQDSWQVTTATYRFDMAIEEDLIEEVARIYGYNNIPNIAPV
AALRMSDHKESDLSLKRVRALLVARGFQEAVTYSFVDPKLQNLVHPGLDAMVLPNPISSEMSAMRLSMFTGLLAAVGYNQ
SRQQSRVRLFETGLRFVPDINAESGVRQQAMLACVITGLQNDEHWAMESKTVDFFDLKGDLEAIIGLTVSASEFSFRGAT
HSALHPGQCAEILRNDRVIGHIGAIHPSLEKPFGLNGKTIVFELELDALLHTSLPLAQAVSKFPANRRDIAVVVDESVSA
GDVMKLIRKVGENQLVGINLFDVYLGKGVEPGKKSLAIALTLQDTTRTLEEKEIAETVGSVVSALKSEFNASLRD

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=795, Percent_Identity=63.5220125786163, Blast_Score=1029, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17533403, Length=570, Percent_Identity=22.1052631578947, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21355211, Length=572, Percent_Identity=21.6783216783217, Blast_Score=80, Evalue=8e-15,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 86384; Mature: 86384

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSESWLREWVNPSVSREALSHQITMAGLEVDDVEAVAADFSGVVIGEVVECGQHPDAD
CCCCHHHHHHHCCCCHHHHHHHHEEEEECCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCC
KLRVTKINVGGSELIDIVCGAPNCRQGLKVAVAMVGAVLPGDFKIKKAKLRGMPSEGMLC
CEEEEEEECCHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCCCEE
SYSELGINVESDGIIELPLDAPLGTDIRDYLQLNDAVIDVDLTANRADCLGMAGLAREVG
EHHHHCCEECCCCEEEEECCCCCCCCHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHH
VLNRQAVVEPQWQAVTATTDAQVTINVKAPDACPRYLGRVVKNVNVKAPTPLWMQEKLRR
HCCCCEECCCCCEEEEEECCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
SGIRSIDPIVDITNFVLIEFGQPMHAFDLAKLTGDIQVRLGTGEEKITLLDGSEVTIPSD
CCCCCCCHHHHHHCEEEEECCCCHHHHHHHHCCCCEEEEECCCCCEEEEECCCEEECCCC
TLVIADDARPLALAGVFGGEYSGVSDTTQDILLECAFFAPLAIMGKSRRLGLHTDSSHRF
EEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHH
ERGVDPEMQHKVIDRATLLVLDICGGEAGPVVEAKSEADLPKPAQIVLRRSKLDRILGHH
HCCCCHHHHHHHHHHHEEEEEEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
VPDTDVVEILQRLGFSVVVGQDSWQVTTATYRFDMAIEEDLIEEVARIYGYNNIPNIAPV
CCCHHHHHHHHHCCCEEEEECCCCEEEEEEEEEECHHHHHHHHHHHHHCCCCCCCCHHHH
AALRMSDHKESDLSLKRVRALLVARGFQEAVTYSFVDPKLQNLVHPGLDAMVLPNPISSE
HHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHCCCCCEEECCCCCHHH
MSAMRLSMFTGLLAAVGYNQSRQQSRVRLFETGLRFVPDINAESGVRQQAMLACVITGLQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHCCC
NDEHWAMESKTVDFFDLKGDLEAIIGLTVSASEFSFRGATHSALHPGQCAEILRNDRVIG
CCCCCEECCCCEEEEECCCCHHHHEEEEEECCCCEECCCCCCCCCCHHHHHHHHCCCEEE
HIGAIHPSLEKPFGLNGKTIVFELELDALLHTSLPLAQAVSKFPANRRDIAVVVDESVSA
ECCCCCCCCCCCCCCCCCEEEEEEEHHHHHHCCCCHHHHHHHCCCCCCCEEEEEECCCCH
GDVMKLIRKVGENQLVGINLFDVYLGKGVEPGKKSLAIALTLQDTTRTLEEKEIAETVGS
HHHHHHHHHCCCCCEEEEEEEEHHHCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHH
VVSALKSEFNASLRD
HHHHHHHHHCCCCCC
>Mature Secondary Structure
MKFSESWLREWVNPSVSREALSHQITMAGLEVDDVEAVAADFSGVVIGEVVECGQHPDAD
CCCCHHHHHHHCCCCHHHHHHHHEEEEECCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCC
KLRVTKINVGGSELIDIVCGAPNCRQGLKVAVAMVGAVLPGDFKIKKAKLRGMPSEGMLC
CEEEEEEECCHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCCCEE
SYSELGINVESDGIIELPLDAPLGTDIRDYLQLNDAVIDVDLTANRADCLGMAGLAREVG
EHHHHCCEECCCCEEEEECCCCCCCCHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHH
VLNRQAVVEPQWQAVTATTDAQVTINVKAPDACPRYLGRVVKNVNVKAPTPLWMQEKLRR
HCCCCEECCCCCEEEEEECCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
SGIRSIDPIVDITNFVLIEFGQPMHAFDLAKLTGDIQVRLGTGEEKITLLDGSEVTIPSD
CCCCCCCHHHHHHCEEEEECCCCHHHHHHHHCCCCEEEEECCCCCEEEEECCCEEECCCC
TLVIADDARPLALAGVFGGEYSGVSDTTQDILLECAFFAPLAIMGKSRRLGLHTDSSHRF
EEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHH
ERGVDPEMQHKVIDRATLLVLDICGGEAGPVVEAKSEADLPKPAQIVLRRSKLDRILGHH
HCCCCHHHHHHHHHHHEEEEEEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
VPDTDVVEILQRLGFSVVVGQDSWQVTTATYRFDMAIEEDLIEEVARIYGYNNIPNIAPV
CCCHHHHHHHHHCCCEEEEECCCCEEEEEEEEEECHHHHHHHHHHHHHCCCCCCCCHHHH
AALRMSDHKESDLSLKRVRALLVARGFQEAVTYSFVDPKLQNLVHPGLDAMVLPNPISSE
HHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHCCCCCEEECCCCCHHH
MSAMRLSMFTGLLAAVGYNQSRQQSRVRLFETGLRFVPDINAESGVRQQAMLACVITGLQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHCCC
NDEHWAMESKTVDFFDLKGDLEAIIGLTVSASEFSFRGATHSALHPGQCAEILRNDRVIG
CCCCCEECCCCEEEEECCCCHHHHEEEEEECCCCEECCCCCCCCCCHHHHHHHHCCCEEE
HIGAIHPSLEKPFGLNGKTIVFELELDALLHTSLPLAQAVSKFPANRRDIAVVVDESVSA
ECCCCCCCCCCCCCCCCCEEEEEEEHHHHHHCCCCHHHHHHHCCCCCCCEEEEEECCCCH
GDVMKLIRKVGENQLVGINLFDVYLGKGVEPGKKSLAIALTLQDTTRTLEEKEIAETVGS
HHHHHHHHHCCCCCEEEEEEEEHHHCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHH
VVSALKSEFNASLRD
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]