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The map label for this gene is glgX [H]

Identifier: 126173570

GI number: 126173570

Start: 1520044

End: 1522245

Strand: Direct

Name: glgX [H]

Synonym: Sbal_1332

Alternate gene names: 126173570

Gene position: 1520044-1522245 (Clockwise)

Preceding gene: 126173569

Following gene: 126173571

Centisome position: 29.65

GC content: 49.23

Gene sequence:

>2202_bases
ATGCAAAAGCCCACAGCTGATGATATCAAGCTCAACCCTGACGCTAAACATAACCCTTCTCAAAATGAGAAATGGGCCTT
GGGAGCCGGTGAGCCTTTTCCGCTCGGTGCGAGTGTCGAGGGTGATGGCGTCAACTTTGCGTTATTTTCAGCTAACGCGA
CAGCGGTCGAGTTATGCCTATTTGATGACAATGGCGAGCAAGAAATCGCGCGGATTGCGTTAACTGAACAAACCCAGCAA
ATCTGGCATGTGTTTGTGCATGGGCTCAAGGCGGGGCAACTTTACGGCTATCGCGTTTATGGCTCCTATGAGCCTCTGCT
TGGCCACAGATTTAACCCCAATAAGTTATTGCTCGACCCTTATGCGCGCCAGCTGGTCGGTGAATATAGCGACCATGAGT
CTAACTATGGTTACGAGCTGAATCACAAGGATGAAGACTTATCTTTCAGTAAGTTAGATAACGCTGCCCATGTGCCAAAA
TGTAAAGTCGTGGACATTCGTCCATTAATCGACATCGCCGCTAAGCAGTCGATTCAGCCCGTAGCGACGAGGTTTCAGCC
CAACCCCCTAGAGCGCAGCATTATTTATGAAATGCACGTTAAAGGCTTTACGGCGGCGCATCCTGAGATTGAACCTAATA
AACGCGCCACCTTTGCGGGTTTGGCGACTCAGCCTGCTATCGATTATTTGGCTGAACTTGGGGTGACGAGCGTCGAGTTA
CTCCCTGTACAGGCCTTTTTCACTGAGCCGTTTTTGCTCGAGAAAAAGCTCACTAATTATTGGGGCTACAACAGTATCGG
TTTTTTTGCCCCTGAGCCGAGTTATCTGTCATCCGACGATATTGGTGAGTTTCGCGCTATGGTCGATGCACTTCACGGTG
CGGGTATCGAAGTGATCCTCGATGTGGTCTATAACCACAGCGCCGAGGGCAGTCGACTTGGGCCAACCTTTAGTTTTCGT
GGCATAGATAATCTCAGTTATTACCGCCTACATCCTAATGATAAGCGTTTTTACATTAACGATACGGGTTGCGGAAACAC
CTTAAATATCAACCATCCACGCATGTTGCAGCTCGTGTTAGATTCGCTGCGTTACTGGGTAGAAATCATGGGCGTCGATG
GATTTCGCTTCGATTTAGCGGCCTGTTTAGGTCGCGAGGCCTATGGTTTTGATCCCGGCTCAGGTTTCTTCGATGCGCTG
TTGCAAGACCCTGTGTTGTGCCGAGTGAAATTGATTGCTGAGCCTTGGGATATTGGCCCTGGCGGTTATCAATTAGGTAA
TTTTCCCGTGGCCTTTAGCGAGTGGAATGACAGATATAGAGACACTATGCGCCGTTTCTGGCGCGGCGATCACAGCATGT
TGCCCGAATTTGCTCGGCGATTTCACGGCTCGGGGGATTTCTTCGAGCATGGCGGCCGTCCACCTGCGACCAGTCTTAAT
TTTTTAACCAGTCACGACGGTTTTACCCTCAAAGATTTAGTGAGTTATACCCAGCGCCACAATTTGGCGAATGGGGAAGA
TAATCGCGACGGTCACCAAGAGAACTTCAGTCATCATTATGGGATTGAAGGGGAAACCGAAGATAGCGCAATTCTGGCAC
TTCGCAGCCGTCAACAGCGTAACTTATTGACGACACTATTTTTATCCCAAGGTGTGCCTATGTTGTTGAGCGGCGATGAA
ACGGGCCGCACCCAGCAAGGTAACAATAATGCCTATTGCCAAGACAATGAACTGAACTGGTTTGATTGGTCGGCAAAGGG
CATGGACACTGAGCTGTTAGCCTTTACTCAGCAGCTTATCGCCCTGCGAAAACGTTTCCCGCTGTTATGCGCTAAACGCT
TCATTCATGAACAGTTATTGGCCGAATCTTTAGCCGATACCGGTGCTCGCTTAGATTGGTTTAGTCGTCAAGGTGAGCAA
ATGACCAAGAGCCTGTGGACTGAGTCTATGTGTCGCAGTTTATCTGTGGTGCTTTCTGGTGACTTAGAAGGCCAAGGTAA
GCAGCAAGCTCTACTGTTGATGGTCAATGCCGACGACAACCCTTTGGCATTTACGCCGCCGACCTTTGAGCACTTGAGTC
CGTGGCAATGCCTGATCCACACCCAAACTGAGCCGCTGGATGCTCAGATCTCTACGACGCGATACTTGCTGCAAGATCGC
AGTCTCATGCTTTTTCATGCTGATTTGATTTTCAAGAAATGA

Upstream 100 bases:

>100_bases
CTGCCGTGGCAGGGCATGGCGCAGAGCGCGCTGATCACTGTGCCGCCCTTGGGTTGTCTAGTGATTGGGCCTGCAACTGG
ATTGGCTGAGGCGAACTAGT

Downstream 100 bases:

>100_bases
AGGAATTAACTATGACTGACGAAAGCGAACTGAGCCCAAATACCAGCGAAAAACCCGTATCATCCGCGCCAAAAGCGGCG
AGTAAGAAGGCGCCAGCTAA

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 733; Mature: 733

Protein sequence:

>733_residues
MQKPTADDIKLNPDAKHNPSQNEKWALGAGEPFPLGASVEGDGVNFALFSANATAVELCLFDDNGEQEIARIALTEQTQQ
IWHVFVHGLKAGQLYGYRVYGSYEPLLGHRFNPNKLLLDPYARQLVGEYSDHESNYGYELNHKDEDLSFSKLDNAAHVPK
CKVVDIRPLIDIAAKQSIQPVATRFQPNPLERSIIYEMHVKGFTAAHPEIEPNKRATFAGLATQPAIDYLAELGVTSVEL
LPVQAFFTEPFLLEKKLTNYWGYNSIGFFAPEPSYLSSDDIGEFRAMVDALHGAGIEVILDVVYNHSAEGSRLGPTFSFR
GIDNLSYYRLHPNDKRFYINDTGCGNTLNINHPRMLQLVLDSLRYWVEIMGVDGFRFDLAACLGREAYGFDPGSGFFDAL
LQDPVLCRVKLIAEPWDIGPGGYQLGNFPVAFSEWNDRYRDTMRRFWRGDHSMLPEFARRFHGSGDFFEHGGRPPATSLN
FLTSHDGFTLKDLVSYTQRHNLANGEDNRDGHQENFSHHYGIEGETEDSAILALRSRQQRNLLTTLFLSQGVPMLLSGDE
TGRTQQGNNNAYCQDNELNWFDWSAKGMDTELLAFTQQLIALRKRFPLLCAKRFIHEQLLAESLADTGARLDWFSRQGEQ
MTKSLWTESMCRSLSVVLSGDLEGQGKQQALLLMVNADDNPLAFTPPTFEHLSPWQCLIHTQTEPLDAQISTTRYLLQDR
SLMLFHADLIFKK

Sequences:

>Translated_733_residues
MQKPTADDIKLNPDAKHNPSQNEKWALGAGEPFPLGASVEGDGVNFALFSANATAVELCLFDDNGEQEIARIALTEQTQQ
IWHVFVHGLKAGQLYGYRVYGSYEPLLGHRFNPNKLLLDPYARQLVGEYSDHESNYGYELNHKDEDLSFSKLDNAAHVPK
CKVVDIRPLIDIAAKQSIQPVATRFQPNPLERSIIYEMHVKGFTAAHPEIEPNKRATFAGLATQPAIDYLAELGVTSVEL
LPVQAFFTEPFLLEKKLTNYWGYNSIGFFAPEPSYLSSDDIGEFRAMVDALHGAGIEVILDVVYNHSAEGSRLGPTFSFR
GIDNLSYYRLHPNDKRFYINDTGCGNTLNINHPRMLQLVLDSLRYWVEIMGVDGFRFDLAACLGREAYGFDPGSGFFDAL
LQDPVLCRVKLIAEPWDIGPGGYQLGNFPVAFSEWNDRYRDTMRRFWRGDHSMLPEFARRFHGSGDFFEHGGRPPATSLN
FLTSHDGFTLKDLVSYTQRHNLANGEDNRDGHQENFSHHYGIEGETEDSAILALRSRQQRNLLTTLFLSQGVPMLLSGDE
TGRTQQGNNNAYCQDNELNWFDWSAKGMDTELLAFTQQLIALRKRFPLLCAKRFIHEQLLAESLADTGARLDWFSRQGEQ
MTKSLWTESMCRSLSVVLSGDLEGQGKQQALLLMVNADDNPLAFTPPTFEHLSPWQCLIHTQTEPLDAQISTTRYLLQDR
SLMLFHADLIFKK
>Mature_733_residues
MQKPTADDIKLNPDAKHNPSQNEKWALGAGEPFPLGASVEGDGVNFALFSANATAVELCLFDDNGEQEIARIALTEQTQQ
IWHVFVHGLKAGQLYGYRVYGSYEPLLGHRFNPNKLLLDPYARQLVGEYSDHESNYGYELNHKDEDLSFSKLDNAAHVPK
CKVVDIRPLIDIAAKQSIQPVATRFQPNPLERSIIYEMHVKGFTAAHPEIEPNKRATFAGLATQPAIDYLAELGVTSVEL
LPVQAFFTEPFLLEKKLTNYWGYNSIGFFAPEPSYLSSDDIGEFRAMVDALHGAGIEVILDVVYNHSAEGSRLGPTFSFR
GIDNLSYYRLHPNDKRFYINDTGCGNTLNINHPRMLQLVLDSLRYWVEIMGVDGFRFDLAACLGREAYGFDPGSGFFDAL
LQDPVLCRVKLIAEPWDIGPGGYQLGNFPVAFSEWNDRYRDTMRRFWRGDHSMLPEFARRFHGSGDFFEHGGRPPATSLN
FLTSHDGFTLKDLVSYTQRHNLANGEDNRDGHQENFSHHYGIEGETEDSAILALRSRQQRNLLTTLFLSQGVPMLLSGDE
TGRTQQGNNNAYCQDNELNWFDWSAKGMDTELLAFTQQLIALRKRFPLLCAKRFIHEQLLAESLADTGARLDWFSRQGEQ
MTKSLWTESMCRSLSVVLSGDLEGQGKQQALLLMVNADDNPLAFTPPTFEHLSPWQCLIHTQTEPLDAQISTTRYLLQDR
SLMLFHADLIFKK

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=635, Percent_Identity=47.8740157480315, Blast_Score=543, Evalue=1e-155,
Organism=Escherichia coli, GI1789839, Length=348, Percent_Identity=25.2873563218391, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 82785; Mature: 82785

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKPTADDIKLNPDAKHNPSQNEKWALGAGEPFPLGASVEGDGVNFALFSANATAVELCL
CCCCCCCCEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEE
FDDNGEQEIARIALTEQTQQIWHVFVHGLKAGQLYGYRVYGSYEPLLGHRFNPNKLLLDP
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHCCCCCCCEEEECH
YARQLVGEYSDHESNYGYELNHKDEDLSFSKLDNAAHVPKCKVVDIRPLIDIAAKQSIQP
HHHHHHHCCCCCCCCCCEECCCCCCCCCHHHCCCCCCCCCEEEEECHHHHHHHHHCCCCH
VATRFQPNPLERSIIYEMHVKGFTAAHPEIEPNKRATFAGLATQPAIDYLAELGVTSVEL
HHHCCCCCCCCHHEEEEEEECCEECCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCEEEE
LPVQAFFTEPFLLEKKLTNYWGYNSIGFFAPEPSYLSSDDIGEFRAMVDALHGAGIEVIL
EEEHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHH
DVVYNHSAEGSRLGPTFSFRGIDNLSYYRLHPNDKRFYINDTGCGNTLNINHPRMLQLVL
HHHHCCCCCCCCCCCEEEECCCCCCEEEEECCCCCEEEEECCCCCCEEECCCHHHHHHHH
DSLRYWVEIMGVDGFRFDLAACLGREAYGFDPGSGFFDALLQDPVLCRVKLIAEPWDIGP
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCCCC
GGYQLGNFPVAFSEWNDRYRDTMRRFWRGDHSMLPEFARRFHGSGDFFEHGGRPPATSLN
CCCCCCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCCEE
FLTSHDGFTLKDLVSYTQRHNLANGEDNRDGHQENFSHHYGIEGETEDSAILALRSRQQR
EEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
NLLTTLFLSQGVPMLLSGDETGRTQQGNNNAYCQDNELNWFDWSAKGMDTELLAFTQQLI
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEECCCCCEEECCCCCCCHHHHHHHHHHH
ALRKRFPLLCAKRFIHEQLLAESLADTGARLDWFSRQGEQMTKSLWTESMCRSLSVVLSG
HHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
DLEGQGKQQALLLMVNADDNPLAFTPPTFEHLSPWQCLIHTQTEPLDAQISTTRYLLQDR
CCCCCCCCEEEEEEEECCCCCEEECCCCHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCC
SLMLFHADLIFKK
CEEEEEEEEHCCC
>Mature Secondary Structure
MQKPTADDIKLNPDAKHNPSQNEKWALGAGEPFPLGASVEGDGVNFALFSANATAVELCL
CCCCCCCCEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEE
FDDNGEQEIARIALTEQTQQIWHVFVHGLKAGQLYGYRVYGSYEPLLGHRFNPNKLLLDP
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHCCCCCCCEEEECH
YARQLVGEYSDHESNYGYELNHKDEDLSFSKLDNAAHVPKCKVVDIRPLIDIAAKQSIQP
HHHHHHHCCCCCCCCCCEECCCCCCCCCHHHCCCCCCCCCEEEEECHHHHHHHHHCCCCH
VATRFQPNPLERSIIYEMHVKGFTAAHPEIEPNKRATFAGLATQPAIDYLAELGVTSVEL
HHHCCCCCCCCHHEEEEEEECCEECCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCEEEE
LPVQAFFTEPFLLEKKLTNYWGYNSIGFFAPEPSYLSSDDIGEFRAMVDALHGAGIEVIL
EEEHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHH
DVVYNHSAEGSRLGPTFSFRGIDNLSYYRLHPNDKRFYINDTGCGNTLNINHPRMLQLVL
HHHHCCCCCCCCCCCEEEECCCCCCEEEEECCCCCEEEEECCCCCCEEECCCHHHHHHHH
DSLRYWVEIMGVDGFRFDLAACLGREAYGFDPGSGFFDALLQDPVLCRVKLIAEPWDIGP
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCCCC
GGYQLGNFPVAFSEWNDRYRDTMRRFWRGDHSMLPEFARRFHGSGDFFEHGGRPPATSLN
CCCCCCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCCEE
FLTSHDGFTLKDLVSYTQRHNLANGEDNRDGHQENFSHHYGIEGETEDSAILALRSRQQR
EEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
NLLTTLFLSQGVPMLLSGDETGRTQQGNNNAYCQDNELNWFDWSAKGMDTELLAFTQQLI
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEECCCCCEEECCCCCCCHHHHHHHHHHH
ALRKRFPLLCAKRFIHEQLLAESLADTGARLDWFSRQGEQMTKSLWTESMCRSLSVVLSG
HHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
DLEGQGKQQALLLMVNADDNPLAFTPPTFEHLSPWQCLIHTQTEPLDAQISTTRYLLQDR
CCCCCCCCEEEEEEEECCCCCEEECCCCHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCC
SLMLFHADLIFKK
CEEEEEEEEHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]