Click here to switch to the map view.

The map label for this gene is tyrS [H]

Identifier: 126173409

GI number: 126173409

Start: 1349816

End: 1351009

Strand: Direct

Name: tyrS [H]

Synonym: Sbal_1170

Alternate gene names: 126173409

Gene position: 1349816-1351009 (Clockwise)

Preceding gene: 126173403

Following gene: 126173417

Centisome position: 26.33

GC content: 47.07

Gene sequence:

>1194_bases
ATGGCTGATTTAGACCAAGTATTAGCAGAAATTAGACGTGGTACCGATGAGATTTTACTCGAAGCGGATCTGCTGGAAAA
ACTCAAAGAAGGACGTCCACTCCGTATTAAGTTAGGCGCCGATCCTACCGCTCCGGACATCCATCTTGGACACACAGTAA
TCTTAAATAAATTAAGGTTATTCCAAGAGTTAGGTCATGAAGTCATTTTCTTGATCGGTGACTTTACCGGTATGGTGGGT
GATCCAAGCGGTAAAAACAGTACGCGTCCACCGTTAACCCGTGAACAAGTATTAGCGAACGCAGAAACCTATAAAGAACA
GGTTTATAAGATACTTGATCCGGTTAAAACCCGTATCGAATTTAACTCAAGCTGGTTAGAGCCATTGGGCGCAGCGGGGA
TGATCCGCCTTGCTTCTAAGCAAACTGTGGCTCGTATGATGGAGCGCGATGACTTTAAGAAGCGTTATGCCTCGGGCCAA
TCTATTGCTATCCATGAGTTTATGTATCCATTACTTCAAGGTTACGACTCAGTTGCGCTGCAGGCCGATGTTGAGCTGGG
TGGTACAGATCAAAAGTTCAACCTGTTAATGGGCCGTGAACTGCAAAAAGCGGAAGGCCAAAAGCCACAAGCTGTGATCA
TGATGCCGCTGCTCGAAGGTTTAGACGGCGTGAAGAAAATGTCTAAGTCGGCGCACAACTATATTGGCGTGAGTGAACCT
GCCAACGAAATGTTTGGCAAGCTGATGTCGATTTCGGACGATTTAATGTGGCGCTACTTTGAGCTGTTGTCATTCCGCCC
ACTGGCTGAAATTGAGCAGTTTAAGCAAGATGTGGCAAATGGGTCTAACCCACGCGACATTAAAATTGCCTTGGCTAAAG
AAATCATCACACGTTTCCACGATGAAGCGTCTGCACAAAGTGCACACCAAGCCTTTGTTGACCGTGCTAAGGGCATGATC
CCTGACGATATCGACGCTGTCGAGTTAGTGGCGGGTGACGGTCTGGCGATTGCTAATCTGTTGAAAGATGCTGATTTAGT
GGCGTCGACTTCAGAAGCCATGCGGATGATCAAACAGGGCGCGGTGAAGATGGACGGCGAAAAGCTGGACGATAGCCGTA
TGATCTTGTCTGCTGGTACTGTTGCGGTATTCCAAGTCGGTAAACGCAAGTTTGCCAAAGTGACTTTGGTTTAA

Upstream 100 bases:

>100_bases
TTAAATAATCCCGTTAGTGTACACACTTTCAATTGTGGTGGCTAACAAGTAACATAAGTCTCTTCATTTTATGATCATGG
CCTTAGGAGTCCCTGCCGAG

Downstream 100 bases:

>100_bases
GTAGCTTTAGTGTAAGTGACTTTGATTTAAGTCGCTTTAGTTCAGATGACTGTGGTTTACCTGACTCTAGTTTGACTTAC
AATGTTTTAAGCCATTGTCT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MADLDQVLAEIRRGTDEILLEADLLEKLKEGRPLRIKLGADPTAPDIHLGHTVILNKLRLFQELGHEVIFLIGDFTGMVG
DPSGKNSTRPPLTREQVLANAETYKEQVYKILDPVKTRIEFNSSWLEPLGAAGMIRLASKQTVARMMERDDFKKRYASGQ
SIAIHEFMYPLLQGYDSVALQADVELGGTDQKFNLLMGRELQKAEGQKPQAVIMMPLLEGLDGVKKMSKSAHNYIGVSEP
ANEMFGKLMSISDDLMWRYFELLSFRPLAEIEQFKQDVANGSNPRDIKIALAKEIITRFHDEASAQSAHQAFVDRAKGMI
PDDIDAVELVAGDGLAIANLLKDADLVASTSEAMRMIKQGAVKMDGEKLDDSRMILSAGTVAVFQVGKRKFAKVTLV

Sequences:

>Translated_397_residues
MADLDQVLAEIRRGTDEILLEADLLEKLKEGRPLRIKLGADPTAPDIHLGHTVILNKLRLFQELGHEVIFLIGDFTGMVG
DPSGKNSTRPPLTREQVLANAETYKEQVYKILDPVKTRIEFNSSWLEPLGAAGMIRLASKQTVARMMERDDFKKRYASGQ
SIAIHEFMYPLLQGYDSVALQADVELGGTDQKFNLLMGRELQKAEGQKPQAVIMMPLLEGLDGVKKMSKSAHNYIGVSEP
ANEMFGKLMSISDDLMWRYFELLSFRPLAEIEQFKQDVANGSNPRDIKIALAKEIITRFHDEASAQSAHQAFVDRAKGMI
PDDIDAVELVAGDGLAIANLLKDADLVASTSEAMRMIKQGAVKMDGEKLDDSRMILSAGTVAVFQVGKRKFAKVTLV
>Mature_396_residues
ADLDQVLAEIRRGTDEILLEADLLEKLKEGRPLRIKLGADPTAPDIHLGHTVILNKLRLFQELGHEVIFLIGDFTGMVGD
PSGKNSTRPPLTREQVLANAETYKEQVYKILDPVKTRIEFNSSWLEPLGAAGMIRLASKQTVARMMERDDFKKRYASGQS
IAIHEFMYPLLQGYDSVALQADVELGGTDQKFNLLMGRELQKAEGQKPQAVIMMPLLEGLDGVKKMSKSAHNYIGVSEPA
NEMFGKLMSISDDLMWRYFELLSFRPLAEIEQFKQDVANGSNPRDIKIALAKEIITRFHDEASAQSAHQAFVDRAKGMIP
DDIDAVELVAGDGLAIANLLKDADLVASTSEAMRMIKQGAVKMDGEKLDDSRMILSAGTVAVFQVGKRKFAKVTLV

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=419, Percent_Identity=25.5369928400955, Blast_Score=112, Evalue=6e-25,
Organism=Escherichia coli, GI1787925, Length=404, Percent_Identity=27.2277227722772, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25145802, Length=304, Percent_Identity=28.9473684210526, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325160, Length=424, Percent_Identity=24.5283018867925, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI20130383, Length=403, Percent_Identity=23.3250620347395, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44002; Mature: 43871

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLDQVLAEIRRGTDEILLEADLLEKLKEGRPLRIKLGADPTAPDIHLGHTVILNKLRL
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
FQELGHEVIFLIGDFTGMVGDPSGKNSTRPPLTREQVLANAETYKEQVYKILDPVKTRIE
HHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
FNSSWLEPLGAAGMIRLASKQTVARMMERDDFKKRYASGQSIAIHEFMYPLLQGYDSVAL
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEE
QADVELGGTDQKFNLLMGRELQKAEGQKPQAVIMMPLLEGLDGVKKMSKSAHNYIGVSEP
EEECCCCCCCCHHHHHHCCHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCC
ANEMFGKLMSISDDLMWRYFELLSFRPLAEIEQFKQDVANGSNPRDIKIALAKEIITRFH
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHH
DEASAQSAHQAFVDRAKGMIPDDIDAVELVAGDGLAIANLLKDADLVASTSEAMRMIKQG
HHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHC
AVKMDGEKLDDSRMILSAGTVAVFQVGKRKFAKVTLV
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
ADLDQVLAEIRRGTDEILLEADLLEKLKEGRPLRIKLGADPTAPDIHLGHTVILNKLRL
CCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
FQELGHEVIFLIGDFTGMVGDPSGKNSTRPPLTREQVLANAETYKEQVYKILDPVKTRIE
HHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
FNSSWLEPLGAAGMIRLASKQTVARMMERDDFKKRYASGQSIAIHEFMYPLLQGYDSVAL
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEE
QADVELGGTDQKFNLLMGRELQKAEGQKPQAVIMMPLLEGLDGVKKMSKSAHNYIGVSEP
EEECCCCCCCCHHHHHHCCHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCC
ANEMFGKLMSISDDLMWRYFELLSFRPLAEIEQFKQDVANGSNPRDIKIALAKEIITRFH
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHH
DEASAQSAHQAFVDRAKGMIPDDIDAVELVAGDGLAIANLLKDADLVASTSEAMRMIKQG
HHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHC
AVKMDGEKLDDSRMILSAGTVAVFQVGKRKFAKVTLV
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]