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The map label for this gene is efpA [H]

Identifier: 125624838

GI number: 125624838

Start: 2048995

End: 2050320

Strand: Reverse

Name: efpA [H]

Synonym: llmg_2066

Alternate gene names: 125624838

Gene position: 2050320-2048995 (Counterclockwise)

Preceding gene: 125624839

Following gene: 125624837

Centisome position: 81.06

GC content: 34.24

Gene sequence:

>1326_bases
ATGAAAAATAAAAAAACTTTTGGCTTAGTCCTCTTTATTATCTTATTTTCCTATTTTTTAATTTTGATGGATAATTCGAT
AATTTTCACAAGTACTGTAAAAATATCTCAGGATTTAAATATGAACGAATCAAGCCTTTCTTGGGTTTCAAATGCCTATA
CCATTACATTTGGAGGTTTTCTTTTGTTGGCAGGACGTTTAGGAGATTTAATGGGTCGGAAAATAATTTTTGTCTCTGGC
CTTTTTATTTTTGGACTATCAAGTTTAGCGGTTGGTCTATCTACGTCAACAGAAATGATGATTATAGCAAGAGCAGTTCA
AGGAATTGGCTCAGCTATCATTGCGCCAACGAGCTTGGCTTTACTGATGGATAGCTATCAGGGAAATTTTCGAATGAAGG
CCATCTCTTACTATGGAGCAACCGCGGGTATTGGTTCAAGTTTTGGTTTAATTCTAGGCGGTTGGCTTACCTCAGCAATT
TCATGGCGTGTCGGCTTTTTATTGAATGTCCCTTTTAGTTTATTACTAATTGTTTTAACGCTTGCGAAAGTTCAACAAAA
TGAAATCAAGCCAAGTAAAATTGATTTTCTTGGAAGTTTATTATCGGTCATTATTTCAGTTCTATTTGTTTACGGAATTA
CCATAAGTGATTTATTAATTATAGTTGCTTCTTTAATTTTGATTTTGGTTTTTCTTTTATGGGAAAAAAGAATTACTTAT
GCTCTCATACCTTTAGAGTTATTTAAGAATCGAACTCGAACAGGTTCTTACATTGCTCGTTTTACTTTTATGATGGCCAT
GTTGACTTACTGGTTTATTCTTCCACAAATTATGCAAAAAATGTATCACTTTTCACCATTAGAGTCAGGGTTTGCTTTTC
TGCCTTTAACACTTGTTAATTTTATTGCTGCTCTATTTTTGCCTAAATTAACAAAAGCTTTGGGAAATAGTAAAGTCATG
ATTTTAGGACAAGTGATTTTACTGACAGGAATGTTTTTGTCATTTATATCACAACCAGAATGGGGATATTTTTATGCAAT
GGCTTTTCAAATGATGATTATTGGTCTTGGGCAAGGCTGGCTACTTGCCCCACTTACAGCGGCAGGTGTGGCAGGAGTTT
CCCCGGAATTATCAGGAGCTGCGTCGGGCTTAACAAATATGATGCATCAACTTGGAGGACCGGTAGGTTTATCAATCGTT
GTTCTATTTAGTTCATCTGCCATTGATTTAAGTGCTTATTATCATTTGATAATGGCTTTCATTACAGTATTTTTACTGAT
AGGATTTTTAGTTTTAATTTTCACGAACCCAGTTAATGAAAAATAA

Upstream 100 bases:

>100_bases
ATCTGTCAGTAAATTTTTTATTTTTTAATATGCTTTATAAGCATAATTAATACTAAAATAAGCATTTTTCATCAGTTGAT
TTTTGTTTTAAAATAAATTC

Downstream 100 bases:

>100_bases
AAGGAGAAAAAATGCAAAAAAAAATCGTACTTAATGATGGAAATATCATTCCTCAATTTGGCTTGGGAGTTTATCAAATT
CCTGAAGGAGAAGCAACTGA

Product: multidrug resistance protein B

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: Efflux protein A [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MKNKKTFGLVLFIILFSYFLILMDNSIIFTSTVKISQDLNMNESSLSWVSNAYTITFGGFLLLAGRLGDLMGRKIIFVSG
LFIFGLSSLAVGLSTSTEMMIIARAVQGIGSAIIAPTSLALLMDSYQGNFRMKAISYYGATAGIGSSFGLILGGWLTSAI
SWRVGFLLNVPFSLLLIVLTLAKVQQNEIKPSKIDFLGSLLSVIISVLFVYGITISDLLIIVASLILILVFLLWEKRITY
ALIPLELFKNRTRTGSYIARFTFMMAMLTYWFILPQIMQKMYHFSPLESGFAFLPLTLVNFIAALFLPKLTKALGNSKVM
ILGQVILLTGMFLSFISQPEWGYFYAMAFQMMIIGLGQGWLLAPLTAAGVAGVSPELSGAASGLTNMMHQLGGPVGLSIV
VLFSSSAIDLSAYYHLIMAFITVFLLIGFLVLIFTNPVNEK

Sequences:

>Translated_441_residues
MKNKKTFGLVLFIILFSYFLILMDNSIIFTSTVKISQDLNMNESSLSWVSNAYTITFGGFLLLAGRLGDLMGRKIIFVSG
LFIFGLSSLAVGLSTSTEMMIIARAVQGIGSAIIAPTSLALLMDSYQGNFRMKAISYYGATAGIGSSFGLILGGWLTSAI
SWRVGFLLNVPFSLLLIVLTLAKVQQNEIKPSKIDFLGSLLSVIISVLFVYGITISDLLIIVASLILILVFLLWEKRITY
ALIPLELFKNRTRTGSYIARFTFMMAMLTYWFILPQIMQKMYHFSPLESGFAFLPLTLVNFIAALFLPKLTKALGNSKVM
ILGQVILLTGMFLSFISQPEWGYFYAMAFQMMIIGLGQGWLLAPLTAAGVAGVSPELSGAASGLTNMMHQLGGPVGLSIV
VLFSSSAIDLSAYYHLIMAFITVFLLIGFLVLIFTNPVNEK
>Mature_441_residues
MKNKKTFGLVLFIILFSYFLILMDNSIIFTSTVKISQDLNMNESSLSWVSNAYTITFGGFLLLAGRLGDLMGRKIIFVSG
LFIFGLSSLAVGLSTSTEMMIIARAVQGIGSAIIAPTSLALLMDSYQGNFRMKAISYYGATAGIGSSFGLILGGWLTSAI
SWRVGFLLNVPFSLLLIVLTLAKVQQNEIKPSKIDFLGSLLSVIISVLFVYGITISDLLIIVASLILILVFLLWEKRITY
ALIPLELFKNRTRTGSYIARFTFMMAMLTYWFILPQIMQKMYHFSPLESGFAFLPLTLVNFIAALFLPKLTKALGNSKVM
ILGQVILLTGMFLSFISQPEWGYFYAMAFQMMIIGLGQGWLLAPLTAAGVAGVSPELSGAASGLTNMMHQLGGPVGLSIV
VLFSSSAIDLSAYYHLIMAFITVFLLIGFLVLIFTNPVNEK

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=455, Percent_Identity=22.4175824175824, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1789042, Length=396, Percent_Identity=25, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI87081983, Length=419, Percent_Identity=26.491646778043, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1788710, Length=409, Percent_Identity=27.6283618581907, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788392, Length=402, Percent_Identity=24.8756218905473, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324954, Length=337, Percent_Identity=28.1899109792285, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323735, Length=253, Percent_Identity=26.8774703557312, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323520, Length=347, Percent_Identity=25.9365994236311, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=230, Percent_Identity=30, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48316; Mature: 48316

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKKTFGLVLFIILFSYFLILMDNSIIFTSTVKISQDLNMNESSLSWVSNAYTITFGGF
CCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHCCEEEEHHHH
LLLAGRLGDLMGRKIIFVSGLFIFGLSSLAVGLSTSTEMMIIARAVQGIGSAIIAPTSLA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
LLMDSYQGNFRMKAISYYGATAGIGSSFGLILGGWLTSAISWRVGFLLNVPFSLLLIVLT
HHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LAKVQQNEIKPSKIDFLGSLLSVIISVLFVYGITISDLLIIVASLILILVFLLWEKRITY
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALIPLELFKNRTRTGSYIARFTFMMAMLTYWFILPQIMQKMYHFSPLESGFAFLPLTLVN
EEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
FIAALFLPKLTKALGNSKVMILGQVILLTGMFLSFISQPEWGYFYAMAFQMMIIGLGQGW
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCH
LLAPLTAAGVAGVSPELSGAASGLTNMMHQLGGPVGLSIVVLFSSSAIDLSAYYHLIMAF
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHEEEEEECCCCHHHHHHHHHHHHH
ITVFLLIGFLVLIFTNPVNEK
HHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKNKKTFGLVLFIILFSYFLILMDNSIIFTSTVKISQDLNMNESSLSWVSNAYTITFGGF
CCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHCCEEEEHHHH
LLLAGRLGDLMGRKIIFVSGLFIFGLSSLAVGLSTSTEMMIIARAVQGIGSAIIAPTSLA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
LLMDSYQGNFRMKAISYYGATAGIGSSFGLILGGWLTSAISWRVGFLLNVPFSLLLIVLT
HHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LAKVQQNEIKPSKIDFLGSLLSVIISVLFVYGITISDLLIIVASLILILVFLLWEKRITY
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALIPLELFKNRTRTGSYIARFTFMMAMLTYWFILPQIMQKMYHFSPLESGFAFLPLTLVN
EEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
FIAALFLPKLTKALGNSKVMILGQVILLTGMFLSFISQPEWGYFYAMAFQMMIIGLGQGW
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCH
LLAPLTAAGVAGVSPELSGAASGLTNMMHQLGGPVGLSIVVLFSSSAIDLSAYYHLIMAF
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHEEEEEECCCCHHHHHHHHHHHHH
ITVFLLIGFLVLIFTNPVNEK
HHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]