BacMap

Definition	Lactococcus lactis subsp. cremoris MG1363, complete genome.
Accession	NC_009004
Length	2,529,478

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The map label for this gene is yqhE [C]

Identifier: 125624837

GI number: 125624837

Start: 2048138

End: 2048983

Strand: Reverse

Name: yqhE [C]

Synonym: llmg_2065

Alternate gene names: 125624837

Gene position: 2048983-2048138 (Counterclockwise)

Preceding gene: 125624838

Following gene: 125624836

Centisome position: 81.0

GC content: 34.75

Gene sequence:

>846_bases
ATGCAAAAAAAAATCGTACTTAATGATGGAAATATCATTCCTCAATTTGGCTTGGGAGTTTATCAAATTCCTGAAGGAGA
AGCAACTGAAAATGCAGTCAAAGAGGCGCTGAGATTGGGATATCGTCATATCGATACCGCCCATGCTTATCAAAACGAAC
GCTCTGTTGGTCGAGCCATTCATTCTTCTGGAATTCCAATTTCTGAAATTTGGATAACTAGTAAACTCTGGCCTTCTGAT
TATGATGATGCTTCACAAGCCATTGAGCGAATGCTTGAACTATTAAATCTTAAGCAAATTGACCTGCTTTTACTTCATCA
GCAGGTTGGAAATTATCGTAAAGCTTGGATTGCTTTGGAAAATGCTGTCAAATCAGGAAAAGTAAAATCAATTGGAATTT
CAAATTTTGATGGGGAGAGATTGGTTGATTTATTTAACTTTGCTGACATTAAACCAAGTGCTCTTCAAGTAGAAACACAT
CCTTACCATCAACAAAAAGAACTCCAAGAATTTCTCAAACCTTATGGGACTAAAATTGAAAGTTGGTATCCTCTTGGACA
CGGCGATCAAAGACTACTTGATGAAAGTCGTTTAAAAAAAATAGCCCAAAAATATAATAAGACAGTGGCTCAGATTATCT
TACGCTGGCATATTCAAGAAGGAATCATAGTTTTTCCACGTTCAACTCGAGTCGAACATTTAGCCGAAAATATCAATATT
TTTGATTTTGAATTATCCTGTACTGAAATCAAAGAAATTCGTAAGTTAGATAAAAAGCAACCTTATTTTACAATGACTTA
TGATGAACAAGTCCAAAATTTTTTGTCATGGAAATTGAAAGATTGA

Upstream 100 bases:

>100_bases
ATTATCATTTGATAATGGCTTTCATTACAGTATTTTTACTGATAGGATTTTTAGTTTTAATTTTCACGAACCCAGTTAAT
GAAAAATAAAAGGAGAAAAA

Downstream 100 bases:

>100_bases
TATTAGATTAAAACAAAGAAAGTTACTGACAGTTTTAAAAATGATATACTTTAAAGAGTTAGAACATTTAAAAGAAAATA
AAAATCAAGCTTAATACGTC

Product: aldo/keto reductase family oxidoreductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MQKKIVLNDGNIIPQFGLGVYQIPEGEATENAVKEALRLGYRHIDTAHAYQNERSVGRAIHSSGIPISEIWITSKLWPSD
YDDASQAIERMLELLNLKQIDLLLLHQQVGNYRKAWIALENAVKSGKVKSIGISNFDGERLVDLFNFADIKPSALQVETH
PYHQQKELQEFLKPYGTKIESWYPLGHGDQRLLDESRLKKIAQKYNKTVAQIILRWHIQEGIIVFPRSTRVEHLAENINI
FDFELSCTEIKEIRKLDKKQPYFTMTYDEQVQNFLSWKLKD

Sequences:

>Translated_281_residues
MQKKIVLNDGNIIPQFGLGVYQIPEGEATENAVKEALRLGYRHIDTAHAYQNERSVGRAIHSSGIPISEIWITSKLWPSD
YDDASQAIERMLELLNLKQIDLLLLHQQVGNYRKAWIALENAVKSGKVKSIGISNFDGERLVDLFNFADIKPSALQVETH
PYHQQKELQEFLKPYGTKIESWYPLGHGDQRLLDESRLKKIAQKYNKTVAQIILRWHIQEGIIVFPRSTRVEHLAENINI
FDFELSCTEIKEIRKLDKKQPYFTMTYDEQVQNFLSWKLKD
>Mature_281_residues
MQKKIVLNDGNIIPQFGLGVYQIPEGEATENAVKEALRLGYRHIDTAHAYQNERSVGRAIHSSGIPISEIWITSKLWPSD
YDDASQAIERMLELLNLKQIDLLLLHQQVGNYRKAWIALENAVKSGKVKSIGISNFDGERLVDLFNFADIKPSALQVETH
PYHQQKELQEFLKPYGTKIESWYPLGHGDQRLLDESRLKKIAQKYNKTVAQIILRWHIQEGIIVFPRSTRVEHLAENINI
FDFELSCTEIKEIRKLDKKQPYFTMTYDEQVQNFLSWKLKD

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=296, Percent_Identity=32.7702702702703, Blast_Score=171, Evalue=5e-43,
Organism=Homo sapiens, GI5174391, Length=296, Percent_Identity=32.7702702702703, Blast_Score=171, Evalue=5e-43,
Organism=Homo sapiens, GI5174695, Length=296, Percent_Identity=34.4594594594595, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI223468663, Length=283, Percent_Identity=34.6289752650177, Blast_Score=161, Evalue=5e-40,
Organism=Homo sapiens, GI4502049, Length=290, Percent_Identity=35.5172413793103, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI310109922, Length=281, Percent_Identity=34.1637010676157, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI5453543, Length=302, Percent_Identity=32.1192052980132, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI310109920, Length=302, Percent_Identity=32.1192052980132, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI45446745, Length=302, Percent_Identity=32.1192052980132, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI4503285, Length=302, Percent_Identity=32.1192052980132, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI93277124, Length=296, Percent_Identity=32.0945945945946, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI291291012, Length=280, Percent_Identity=31.7857142857143, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI300116273, Length=278, Percent_Identity=33.0935251798561, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI24497583, Length=301, Percent_Identity=31.2292358803987, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI24497585, Length=306, Percent_Identity=30.3921568627451, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI300116271, Length=273, Percent_Identity=34.0659340659341, Blast_Score=137, Evalue=8e-33,
Organism=Homo sapiens, GI310109924, Length=158, Percent_Identity=31.0126582278481, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI310109926, Length=115, Percent_Identity=40, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI207028673, Length=115, Percent_Identity=40, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI87082198, Length=257, Percent_Identity=39.2996108949416, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1786400, Length=254, Percent_Identity=34.6456692913386, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788081, Length=283, Percent_Identity=27.5618374558304, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17537077, Length=294, Percent_Identity=34.6938775510204, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17537075, Length=294, Percent_Identity=34.6938775510204, Blast_Score=170, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17564128, Length=303, Percent_Identity=33.993399339934, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71998625, Length=270, Percent_Identity=34.0740740740741, Blast_Score=154, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17538386, Length=276, Percent_Identity=33.695652173913, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17537079, Length=294, Percent_Identity=29.9319727891156, Blast_Score=143, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17566692, Length=281, Percent_Identity=33.4519572953737, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17561298, Length=279, Percent_Identity=30.4659498207885, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17552492, Length=265, Percent_Identity=30.5660377358491, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17562292, Length=278, Percent_Identity=29.8561151079137, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17561300, Length=279, Percent_Identity=28.673835125448, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17550248, Length=311, Percent_Identity=26.6881028938907, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6324694, Length=289, Percent_Identity=35.6401384083045, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6320576, Length=283, Percent_Identity=33.2155477031802, Blast_Score=151, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6322556, Length=269, Percent_Identity=33.8289962825279, Blast_Score=146, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6319625, Length=293, Percent_Identity=32.4232081911263, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6321896, Length=308, Percent_Identity=31.1688311688312, Blast_Score=133, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320079, Length=283, Percent_Identity=31.4487632508834, Blast_Score=119, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24662789, Length=299, Percent_Identity=33.4448160535117, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24644950, Length=299, Percent_Identity=31.7725752508361, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24663317, Length=284, Percent_Identity=34.8591549295775, Blast_Score=154, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24657054, Length=272, Percent_Identity=34.9264705882353, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI45553081, Length=290, Percent_Identity=32.7586206896552, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI20129731, Length=298, Percent_Identity=31.8791946308725, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI221378297, Length=319, Percent_Identity=29.7805642633229, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24662785, Length=278, Percent_Identity=38.1294964028777, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24662781, Length=277, Percent_Identity=36.101083032491, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI21356425, Length=301, Percent_Identity=30.5647840531561, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI281366140, Length=252, Percent_Identity=32.5396825396825, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 32591; Mature: 32591

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKIVLNDGNIIPQFGLGVYQIPEGEATENAVKEALRLGYRHIDTAHAYQNERSVGRAI
CCCEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HSSGIPISEIWITSKLWPSDYDDASQAIERMLELLNLKQIDLLLLHQQVGNYRKAWIALE
HHCCCCHHHEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHH
NAVKSGKVKSIGISNFDGERLVDLFNFADIKPSALQVETHPYHQQKELQEFLKPYGTKIE
HHHHCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCC
SWYPLGHGDQRLLDESRLKKIAQKYNKTVAQIILRWHIQEGIIVFPRSTRVEHLAENINI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCE
FDFELSCTEIKEIRKLDKKQPYFTMTYDEQVQNFLSWKLKD
EEEECCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQKKIVLNDGNIIPQFGLGVYQIPEGEATENAVKEALRLGYRHIDTAHAYQNERSVGRAI
CCCEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HSSGIPISEIWITSKLWPSDYDDASQAIERMLELLNLKQIDLLLLHQQVGNYRKAWIALE
HHCCCCHHHEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHH
NAVKSGKVKSIGISNFDGERLVDLFNFADIKPSALQVETHPYHQQKELQEFLKPYGTKIE
HHHHCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCC
SWYPLGHGDQRLLDESRLKKIAQKYNKTVAQIILRWHIQEGIIVFPRSTRVEHLAENINI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCE
FDFELSCTEIKEIRKLDKKQPYFTMTYDEQVQNFLSWKLKD
EEEECCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA