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The map label for this gene is metF [H]

Identifier: 125624052

GI number: 125624052

Start: 1197480

End: 1198337

Strand: Direct

Name: metF [H]

Synonym: llmg_1226

Alternate gene names: 125624052

Gene position: 1197480-1198337 (Clockwise)

Preceding gene: 125624051

Following gene: 125624053

Centisome position: 47.34

GC content: 33.22

Gene sequence:

>858_bases
ATGACAAGTGATTCTAAAATTCTATCTTTTGAAGTTTTTCCTCCAACAACTCAAGTTGGCAGTTCTCATTTAGTGAAAAC
ACTTGATAGTTTAAGAGCGCTTTCTCCTGACTTTATCAGCGTTACCTGTAGTAATAATAATTATGAGAATATTGCTGATA
CAACCATAAAATTTGCAGATTATGTTAATAATACATTAAATATTCCTGCTGTTGCTCATTTACCCGCGGCCTATCTCGAC
AAAACCCAAGTGATAGAGATTTTGGAACGTTTAAGAGAAAAGCAGACTATGAAAGTTTTAGCACTGAGAGGTGATATAAG
TGATGAAGCAACTAAAAAAGACTTCAAATATGCTAGTGACTTGGTAAAATTTATAAAGGATTATGATAGTAGTTTTGAAG
TTTTGGGCGCTTGTTATCCAGATGTTCACCCTGAATCAATAAATCGTATCAGTGATTTTCATTATTTGAAAGAGAAAGTT
GATTCAGGGGCTGATAAGTTAATTACTCAGTTATTTTTTGATAATAATAGCTTCTATGATTTTCAAGAACGTTGTGCCAT
TGCGGAAATAAATGTCCCCATTCTTGCTGGAATTATGCCCGTCATAAACCGTAATCAGATTCTGCGTTTATTAAAAAATT
GCAATACACCTCTACCAGCAAAATTTATCAGAATACTGGAAAAGTATGAACATAATCCTGTTGCGCTAAGAGATGCAGGA
ATCGCTTATGCAATAGATCAAATAGTGGATTTAGTAACTGAAGATGTTGCCGGAATTCATCTATATACAATGAATAATTC
GGAAACCGCACGTTCCATTCATTCTTCAATCTCATCTTTATTTAAAATAAATACATAA

Upstream 100 bases:

>100_bases
CCTGATTGTGGTCTTAAAACAAGAGGGAATAAGGAAACAATCCCTAGTTTGACTCACCTGGTTGAGGCAACAAAAGAGGT
AAGGAAGGGTAAGATAACAT

Downstream 100 bases:

>100_bases
TTATTGTTATGTATTTACTGAAATTGGAGAAAAATTTAGAAAAAGTAGATAAATAATTGACAAATCAAAAAATTTTAATT
ATACTAGCTATTAATATAAA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTSDSKILSFEVFPPTTQVGSSHLVKTLDSLRALSPDFISVTCSNNNYENIADTTIKFADYVNNTLNIPAVAHLPAAYLD
KTQVIEILERLREKQTMKVLALRGDISDEATKKDFKYASDLVKFIKDYDSSFEVLGACYPDVHPESINRISDFHYLKEKV
DSGADKLITQLFFDNNSFYDFQERCAIAEINVPILAGIMPVINRNQILRLLKNCNTPLPAKFIRILEKYEHNPVALRDAG
IAYAIDQIVDLVTEDVAGIHLYTMNNSETARSIHSSISSLFKINT

Sequences:

>Translated_285_residues
MTSDSKILSFEVFPPTTQVGSSHLVKTLDSLRALSPDFISVTCSNNNYENIADTTIKFADYVNNTLNIPAVAHLPAAYLD
KTQVIEILERLREKQTMKVLALRGDISDEATKKDFKYASDLVKFIKDYDSSFEVLGACYPDVHPESINRISDFHYLKEKV
DSGADKLITQLFFDNNSFYDFQERCAIAEINVPILAGIMPVINRNQILRLLKNCNTPLPAKFIRILEKYEHNPVALRDAG
IAYAIDQIVDLVTEDVAGIHLYTMNNSETARSIHSSISSLFKINT
>Mature_284_residues
TSDSKILSFEVFPPTTQVGSSHLVKTLDSLRALSPDFISVTCSNNNYENIADTTIKFADYVNNTLNIPAVAHLPAAYLDK
TQVIEILERLREKQTMKVLALRGDISDEATKKDFKYASDLVKFIKDYDSSFEVLGACYPDVHPESINRISDFHYLKEKVD
SGADKLITQLFFDNNSFYDFQERCAIAEINVPILAGIMPVINRNQILRLLKNCNTPLPAKFIRILEKYEHNPVALRDAGI
AYAIDQIVDLVTEDVAGIHLYTMNNSETARSIHSSISSLFKINT

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=272, Percent_Identity=30.5147058823529, Blast_Score=138, Evalue=4e-33,
Organism=Escherichia coli, GI1790377, Length=266, Percent_Identity=33.0827067669173, Blast_Score=143, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI25147441, Length=286, Percent_Identity=28.3216783216783, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI25147436, Length=286, Percent_Identity=28.3216783216783, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6325234, Length=281, Percent_Identity=31.6725978647687, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6321313, Length=274, Percent_Identity=28.8321167883212, Blast_Score=112, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 32075; Mature: 31944

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDSKILSFEVFPPTTQVGSSHLVKTLDSLRALSPDFISVTCSNNNYENIADTTIKFAD
CCCCCCEEEEEECCCCHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHH
YVNNTLNIPAVAHLPAAYLDKTQVIEILERLREKQTMKVLALRGDISDEATKKDFKYASD
HHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHH
LVKFIKDYDSSFEVLGACYPDVHPESINRISDFHYLKEKVDSGADKLITQLFFDNNSFYD
HHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
FQERCAIAEINVPILAGIMPVINRNQILRLLKNCNTPLPAKFIRILEKYEHNPVALRDAG
HHHCCEEEECCCHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCC
IAYAIDQIVDLVTEDVAGIHLYTMNNSETARSIHSSISSLFKINT
HHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSDSKILSFEVFPPTTQVGSSHLVKTLDSLRALSPDFISVTCSNNNYENIADTTIKFAD
CCCCCEEEEEECCCCHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHH
YVNNTLNIPAVAHLPAAYLDKTQVIEILERLREKQTMKVLALRGDISDEATKKDFKYASD
HHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHH
LVKFIKDYDSSFEVLGACYPDVHPESINRISDFHYLKEKVDSGADKLITQLFFDNNSFYD
HHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
FQERCAIAEINVPILAGIMPVINRNQILRLLKNCNTPLPAKFIRILEKYEHNPVALRDAG
HHHCCEEEECCCHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCC
IAYAIDQIVDLVTEDVAGIHLYTMNNSETARSIHSSISSLFKINT
HHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]