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The map label for this gene is metE

Identifier: 125624051

GI number: 125624051

Start: 1195217

End: 1197490

Strand: Direct

Name: metE

Synonym: llmg_1225

Alternate gene names: 125624051

Gene position: 1195217-1197490 (Clockwise)

Preceding gene: 125624050

Following gene: 125624052

Centisome position: 47.25

GC content: 34.7

Gene sequence:

>2274_bases
ATGAAAAAATCAATTATTGCCTTCCCTCGTATCGGTTCAAATCGTGAATTGAAATTTGCTCTGGAAAAATACTTCCGTAA
AGAAATTTCTGAGGATGAATTACAAATTGTGGCCAAAGAGCTTCGTTTAGAAAGTTGGAAAAGTCAAAAAGAAGCTGGAA
TTGATTACCCCATTTCCAACGATTTCTCTTTCTATGACCAAACTTTAGACCTATCTATTGCACTTGGGGTAATTCCCGAA
CGCTATAAAAAATTAAAACTCAATGAATTAGATACTTTATTTGCTTTAGCGCGTGGTTTTCAAGACGAAGAAAATGACGT
GAAAGCAAGGCCGATGAAAAAATGGTTTAATACAAATTACCATTATATCGTTCCAGAAATTAGCAAAGAAACAGTAATAA
AAGCAAATTTTTCAAAACTATTAAACGAATATCAAGAAGCAAAGACTGCTGGTTTTGAAACTAGACCGACAATTATTGGT
CCATATACCTTTTTAATTTTGGCGGATTACCTTTCTGGAGTAACCGAGGACGCGATATTGTCTGACCTCATTGGAGCCTA
CACAATATTATTTGACCAACTGAATAATTTAGGAGGGGAATGGTTACAAATTGAAGAGCCAGCTCTAGTTTTAGATCAAA
CAGAAGAAGAACAACAGTTATTCATAAAAATTTATCAAGAGCTACTTAAGAATAAAAATAAGCTTAGGGTTCTCCTTCAA
ACCTATTTTGGTGACCTACGCAATAGTTATCAAGAAATCATAAAGCTCGATTTTGATGGGATTGGCCTTGATTTTGTTGA
AGGAAGAGAGTCTGTAAAACTAGTTCAAAAGTATGGTTTTCCTAAAAATAAATTACTCTTTGCAGGAGTTGTCAATGGGA
AAAATATTTGGCGTAATCATTATCAAAAAACACTGAGTTTGCTAAAAGATTTAGGAAACATTGATAATATTGTAATCAAT
ACAAGTTGTTCTTTACAGCATGTTCCAGTAACAACTGAGAATGAAACCAAATTAAGCAAAGAAATTCTCAACCATTTCGC
ATTTGCCAAAGAAAAATTGGTGGAAGTTAGTGAAATTTCCGAAATTTATGTTAAGAAAAATACTTCCCTGTTAGATAAGA
ATATTGCACTTTTTGATAAAAATAGAGTCCAAGAAAATATTCAACTTAAACAAAAAATTATTCATTTAACTGATAAAGAT
TTTATTAGAACGCCTAGCTTAGTTGAAAGACGTGCCGATCAAATAAAAGCACTCAATCTTCCACTGCTGCCAACAACGAC
TATCGGTTCATTTCCTCAAACGCCAGAAATAAGAAAATCTCGATTGCAGTATAAGCGTGGTGAACTGAGCAAAAGCGATT
ATGAAGCATTTTTGGAAGAAAAAATTAAAGAATGTTTGGAACTTCAAGAGAACATTGGGTTAGATGTTTTAGTTCATGGT
GAATTTGAAAGAAATGATATGGTAGAATATTTTGGAGAACAATTAGATGGTTATATTTTCACACAAAAAGCATGGGTTCA
ATCTTATGGAACACGATGTGTTAAACCGCCAATTGTTTGGGGAGATATCACAAGACCGCAAGCTATGACCGTTCGCTGGT
CAGCTTATGCTCAATCTCAGACTTCTAAACCGGTAAAGGGGATGCTGACAGGGCCTGTAACTATTCTAAATTGGTCTTTT
CCAAGAGAAGATATTTCCTTAAAGGAAAGCACTTTACAGTTAGCCTTAGCTGTCCAAGAAGAAGTACTCGATTTAGAAAA
GTCTGGAGTTAAAATCATTCAGATCGATGAAGCAGCACTTAGAGAAAAACTACCATTACGTCGAAGTGATTGGTATAGTG
AATATTTAGATTGGGCGATTCCAGCCTTTCGATTGGTCCATTCTAAGGTGAAAGCTGAAACTCAAATCCATACACACATG
TGTTATTCTGAATTTGAAGATATTATTCCTTCTATTGATGCGATGGATGCCGATGTCATTTCTTTTGAGGCTAGTCGCAG
CCAGTTATCAATTATTGATGCACTAAAAGCTCATCATTTTCAAACTTTGGTAGGACCAGGAGTTTATGATATCCATTCCC
CAAGAATTCCTAGTAGTCAAGAAATAAAAATTCAACTAGAAAAGATTTTGAATAAATTACCAATTGAGCAAGTTTGGGTT
AATCCTGATTGTGGTCTTAAAACAAGAGGGAATAAGGAAACAATCCCTAGTTTGACTCACCTGGTTGAGGCAACAAAAGA
GGTAAGGAAGGGTAAGATAACATATGACAAGTGA

Upstream 100 bases:

>100_bases
TAATATAGTTAAAAACTATAGGTCATCATATCAAAAAGGTATTATTAAAGTTTTATTAAATTTGATAAGATAGACCTATT
AAAAATTGGAGACAGCTACT

Downstream 100 bases:

>100_bases
TTCTAAAATTCTATCTTTTGAAGTTTTTCCTCCAACAACTCAAGTTGGCAGTTCTCATTTAGTGAAAACACTTGATAGTT
TAAGAGCGCTTTCTCCTGAC

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 757; Mature: 757

Protein sequence:

>757_residues
MKKSIIAFPRIGSNRELKFALEKYFRKEISEDELQIVAKELRLESWKSQKEAGIDYPISNDFSFYDQTLDLSIALGVIPE
RYKKLKLNELDTLFALARGFQDEENDVKARPMKKWFNTNYHYIVPEISKETVIKANFSKLLNEYQEAKTAGFETRPTIIG
PYTFLILADYLSGVTEDAILSDLIGAYTILFDQLNNLGGEWLQIEEPALVLDQTEEEQQLFIKIYQELLKNKNKLRVLLQ
TYFGDLRNSYQEIIKLDFDGIGLDFVEGRESVKLVQKYGFPKNKLLFAGVVNGKNIWRNHYQKTLSLLKDLGNIDNIVIN
TSCSLQHVPVTTENETKLSKEILNHFAFAKEKLVEVSEISEIYVKKNTSLLDKNIALFDKNRVQENIQLKQKIIHLTDKD
FIRTPSLVERRADQIKALNLPLLPTTTIGSFPQTPEIRKSRLQYKRGELSKSDYEAFLEEKIKECLELQENIGLDVLVHG
EFERNDMVEYFGEQLDGYIFTQKAWVQSYGTRCVKPPIVWGDITRPQAMTVRWSAYAQSQTSKPVKGMLTGPVTILNWSF
PREDISLKESTLQLALAVQEEVLDLEKSGVKIIQIDEAALREKLPLRRSDWYSEYLDWAIPAFRLVHSKVKAETQIHTHM
CYSEFEDIIPSIDAMDADVISFEASRSQLSIIDALKAHHFQTLVGPGVYDIHSPRIPSSQEIKIQLEKILNKLPIEQVWV
NPDCGLKTRGNKETIPSLTHLVEATKEVRKGKITYDK

Sequences:

>Translated_757_residues
MKKSIIAFPRIGSNRELKFALEKYFRKEISEDELQIVAKELRLESWKSQKEAGIDYPISNDFSFYDQTLDLSIALGVIPE
RYKKLKLNELDTLFALARGFQDEENDVKARPMKKWFNTNYHYIVPEISKETVIKANFSKLLNEYQEAKTAGFETRPTIIG
PYTFLILADYLSGVTEDAILSDLIGAYTILFDQLNNLGGEWLQIEEPALVLDQTEEEQQLFIKIYQELLKNKNKLRVLLQ
TYFGDLRNSYQEIIKLDFDGIGLDFVEGRESVKLVQKYGFPKNKLLFAGVVNGKNIWRNHYQKTLSLLKDLGNIDNIVIN
TSCSLQHVPVTTENETKLSKEILNHFAFAKEKLVEVSEISEIYVKKNTSLLDKNIALFDKNRVQENIQLKQKIIHLTDKD
FIRTPSLVERRADQIKALNLPLLPTTTIGSFPQTPEIRKSRLQYKRGELSKSDYEAFLEEKIKECLELQENIGLDVLVHG
EFERNDMVEYFGEQLDGYIFTQKAWVQSYGTRCVKPPIVWGDITRPQAMTVRWSAYAQSQTSKPVKGMLTGPVTILNWSF
PREDISLKESTLQLALAVQEEVLDLEKSGVKIIQIDEAALREKLPLRRSDWYSEYLDWAIPAFRLVHSKVKAETQIHTHM
CYSEFEDIIPSIDAMDADVISFEASRSQLSIIDALKAHHFQTLVGPGVYDIHSPRIPSSQEIKIQLEKILNKLPIEQVWV
NPDCGLKTRGNKETIPSLTHLVEATKEVRKGKITYDK
>Mature_757_residues
MKKSIIAFPRIGSNRELKFALEKYFRKEISEDELQIVAKELRLESWKSQKEAGIDYPISNDFSFYDQTLDLSIALGVIPE
RYKKLKLNELDTLFALARGFQDEENDVKARPMKKWFNTNYHYIVPEISKETVIKANFSKLLNEYQEAKTAGFETRPTIIG
PYTFLILADYLSGVTEDAILSDLIGAYTILFDQLNNLGGEWLQIEEPALVLDQTEEEQQLFIKIYQELLKNKNKLRVLLQ
TYFGDLRNSYQEIIKLDFDGIGLDFVEGRESVKLVQKYGFPKNKLLFAGVVNGKNIWRNHYQKTLSLLKDLGNIDNIVIN
TSCSLQHVPVTTENETKLSKEILNHFAFAKEKLVEVSEISEIYVKKNTSLLDKNIALFDKNRVQENIQLKQKIIHLTDKD
FIRTPSLVERRADQIKALNLPLLPTTTIGSFPQTPEIRKSRLQYKRGELSKSDYEAFLEEKIKECLELQENIGLDVLVHG
EFERNDMVEYFGEQLDGYIFTQKAWVQSYGTRCVKPPIVWGDITRPQAMTVRWSAYAQSQTSKPVKGMLTGPVTILNWSF
PREDISLKESTLQLALAVQEEVLDLEKSGVKIIQIDEAALREKLPLRRSDWYSEYLDWAIPAFRLVHSKVKAETQIHTHM
CYSEFEDIIPSIDAMDADVISFEASRSQLSIIDALKAHHFQTLVGPGVYDIHSPRIPSSQEIKIQLEKILNKLPIEQVWV
NPDCGLKTRGNKETIPSLTHLVEATKEVRKGKITYDK

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=753, Percent_Identity=45.0199203187251, Blast_Score=650, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=779, Percent_Identity=45.3145057766367, Blast_Score=643, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_LACLM (A2RKK4)

Other databases:

- EMBL:   AM406671
- RefSeq:   YP_001032534.1
- ProteinModelPortal:   A2RKK4
- SMR:   A2RKK4
- STRING:   A2RKK4
- GeneID:   4796963
- GenomeReviews:   AM406671_GR
- KEGG:   llm:llmg_1225
- eggNOG:   COG0620
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 87117; Mature: 87117

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSIIAFPRIGSNRELKFALEKYFRKEISEDELQIVAKELRLESWKSQKEAGIDYPISN
CCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DFSFYDQTLDLSIALGVIPERYKKLKLNELDTLFALARGFQDEENDVKARPMKKWFNTNY
CCHHHHHHCCEEEEECCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCC
HYIVPEISKETVIKANFSKLLNEYQEAKTAGFETRPTIIGPYTFLILADYLSGVTEDAIL
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHCCCCHHHHH
SDLIGAYTILFDQLNNLGGEWLQIEEPALVLDQTEEEQQLFIKIYQELLKNKNKLRVLLQ
HHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHH
TYFGDLRNSYQEIIKLDFDGIGLDFVEGRESVKLVQKYGFPKNKLLFAGVVNGKNIWRNH
HHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
YQKTLSLLKDLGNIDNIVINTSCSLQHVPVTTENETKLSKEILNHFAFAKEKLVEVSEIS
HHHHHHHHHHCCCCCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EIYVKKNTSLLDKNIALFDKNRVQENIQLKQKIIHLTDKDFIRTPSLVERRADQIKALNL
HHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHCCC
PLLPTTTIGSFPQTPEIRKSRLQYKRGELSKSDYEAFLEEKIKECLELQENIGLDVLVHG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
EFERNDMVEYFGEQLDGYIFTQKAWVQSYGTRCVKPPIVWGDITRPQAMTVRWSAYAQSQ
CCCCHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCCCEEECCCCCCCEEEEEEHHHHHCC
TSKPVKGMLTGPVTILNWSFPREDISLKESTLQLALAVQEEVLDLEKSGVKIIQIDEAAL
CCCCCCCEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHH
REKLPLRRSDWYSEYLDWAIPAFRLVHSKVKAETQIHTHMCYSEFEDIIPSIDAMDADVI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
SFEASRSQLSIIDALKAHHFQTLVGPGVYDIHSPRIPSSQEIKIQLEKILNKLPIEQVWV
EECCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEE
NPDCGLKTRGNKETIPSLTHLVEATKEVRKGKITYDK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKSIIAFPRIGSNRELKFALEKYFRKEISEDELQIVAKELRLESWKSQKEAGIDYPISN
CCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DFSFYDQTLDLSIALGVIPERYKKLKLNELDTLFALARGFQDEENDVKARPMKKWFNTNY
CCHHHHHHCCEEEEECCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCC
HYIVPEISKETVIKANFSKLLNEYQEAKTAGFETRPTIIGPYTFLILADYLSGVTEDAIL
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHCCCCHHHHH
SDLIGAYTILFDQLNNLGGEWLQIEEPALVLDQTEEEQQLFIKIYQELLKNKNKLRVLLQ
HHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHH
TYFGDLRNSYQEIIKLDFDGIGLDFVEGRESVKLVQKYGFPKNKLLFAGVVNGKNIWRNH
HHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
YQKTLSLLKDLGNIDNIVINTSCSLQHVPVTTENETKLSKEILNHFAFAKEKLVEVSEIS
HHHHHHHHHHCCCCCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EIYVKKNTSLLDKNIALFDKNRVQENIQLKQKIIHLTDKDFIRTPSLVERRADQIKALNL
HHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHCCC
PLLPTTTIGSFPQTPEIRKSRLQYKRGELSKSDYEAFLEEKIKECLELQENIGLDVLVHG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
EFERNDMVEYFGEQLDGYIFTQKAWVQSYGTRCVKPPIVWGDITRPQAMTVRWSAYAQSQ
CCCCHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCCCEEECCCCCCCEEEEEEHHHHHCC
TSKPVKGMLTGPVTILNWSFPREDISLKESTLQLALAVQEEVLDLEKSGVKIIQIDEAAL
CCCCCCCEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHH
REKLPLRRSDWYSEYLDWAIPAFRLVHSKVKAETQIHTHMCYSEFEDIIPSIDAMDADVI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
SFEASRSQLSIIDALKAHHFQTLVGPGVYDIHSPRIPSSQEIKIQLEKILNKLPIEQVWV
EECCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEE
NPDCGLKTRGNKETIPSLTHLVEATKEVRKGKITYDK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA