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The map label for this gene is glyA [H]

Identifier: 125623428

GI number: 125623428

Start: 555748

End: 556995

Strand: Direct

Name: glyA [H]

Synonym: llmg_0563

Alternate gene names: 125623428

Gene position: 555748-556995 (Clockwise)

Preceding gene: 125623427

Following gene: 125623429

Centisome position: 21.97

GC content: 39.1

Gene sequence:

>1248_bases
ATGATTTTTGATAAAGAAGATTTTGAAAGCTTTGACCCAGAATTGTGGGCGGCCATTCATGCTGAGGAAATTCGTCAGCA
ACAAAATATTGAATTAATTGCTTCTGAAAATATTGTTTCAAAGGCAGTGATGGCAGCACAAGGTTCTGTTTTGACGAATA
AATATGCCGAAGGATACCCAGGAAAACGTTACTATGGTGGTACAGAAGCGGTTGATGTTGTGGAAAATTTGGCAATTGAC
CGTGCAAAAGAACTTTTCGGTGCAAAATTTGCCAATGTTCAACCTCACTCAGGTTCTCAAGCCAATGCTGCAGCCTATAT
GGCATTAATTCAACCAGGAGATACAGTTCTGGGAATGGATTTAAACGCTGGTGGTCATTTGACGCACGGAGCTTCAGTCA
ATTTTTCAGGAAAAACTTATCATTTTGTCCCTTATGGGGTAAATCCTCAAACAGAGCTTTTAGATTATGAAGAAATTCTT
AAAATTGCTAAAGAAGTTCAACCTAAACTGATTGTGGCAGGGGCTTCTGCCTATTCACGTTTGATTGATTTTGCTAAATT
CCGTCAGATTGCTGATAGCGTTGGTGCGAAATTAATGGTTGATATGGCTCATATTGCTGGTTTAGTGGCTACTGGTGCTC
ACCCCAATCCACTTCCTTATGCTGATGTTGTCACAACAACAACTCATAAAACTTTAAGAGGCCCACGCGGTGGAATGATT
TTAACCAATGATGAAGTCTTGGCTAAAAAAATCAATTCCGCAATTTTCCCTGGAACGCAAGGTGGACCTTTAGAACACGT
GATTGCTGCTAAAGCAGTCGCTTTTAAAGAAGCACTTGACCCAGAATTTGCTACATATATTGAACAAGTCATTAAAAATA
CTCAAGCAATGGCTGATGAGTTTGCTAAGGTTGATGGACTACGTTTGATCGCAGGAGGCTCAGATAATCACTTACTTAAT
TTGAAGGTCTTAGATCTTGGAATTAATGGGAAAGAAGCACAGGACTTATTAGATAGTGTTCATATCACGCTTAATAAAGA
GGCCATACCAGACGAAACTTTATCTCCATTTAAAACCTCAGGAGTGCGAATTGGTGCGGCAGCAATCACATCAAGAGGAT
TTAAGGAAGCTGAAGCTCGAAAAGTTGCTCAATTAGTTTCTAAAGCCTTGGTTAATCATGACAACCAAGAAAAACTTGAA
GAAGTTAGAAAATCTGCCTTAGAGTTAACACATCAATTTCCATTATAA

Upstream 100 bases:

>100_bases
AGTAAATCATAATTTAACTGATTAAGTTTATAGCGTTTAAGACTATAAAATTCTACTTATATTTTTAAAAATATCCCCAA
TAGACATACAAGGAGAACGA

Downstream 100 bases:

>100_bases
TTTATAAAAATTAGACAATAATTTTTGTCTAATTTTTTTATATTAAGTTAAATTTAAACGAGAAGTGTTATAATAGAACT
TATATAAAAATGACCGCATA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MIFDKEDFESFDPELWAAIHAEEIRQQQNIELIASENIVSKAVMAAQGSVLTNKYAEGYPGKRYYGGTEAVDVVENLAID
RAKELFGAKFANVQPHSGSQANAAAYMALIQPGDTVLGMDLNAGGHLTHGASVNFSGKTYHFVPYGVNPQTELLDYEEIL
KIAKEVQPKLIVAGASAYSRLIDFAKFRQIADSVGAKLMVDMAHIAGLVATGAHPNPLPYADVVTTTTHKTLRGPRGGMI
LTNDEVLAKKINSAIFPGTQGGPLEHVIAAKAVAFKEALDPEFATYIEQVIKNTQAMADEFAKVDGLRLIAGGSDNHLLN
LKVLDLGINGKEAQDLLDSVHITLNKEAIPDETLSPFKTSGVRIGAAAITSRGFKEAEARKVAQLVSKALVNHDNQEKLE
EVRKSALELTHQFPL

Sequences:

>Translated_415_residues
MIFDKEDFESFDPELWAAIHAEEIRQQQNIELIASENIVSKAVMAAQGSVLTNKYAEGYPGKRYYGGTEAVDVVENLAID
RAKELFGAKFANVQPHSGSQANAAAYMALIQPGDTVLGMDLNAGGHLTHGASVNFSGKTYHFVPYGVNPQTELLDYEEIL
KIAKEVQPKLIVAGASAYSRLIDFAKFRQIADSVGAKLMVDMAHIAGLVATGAHPNPLPYADVVTTTTHKTLRGPRGGMI
LTNDEVLAKKINSAIFPGTQGGPLEHVIAAKAVAFKEALDPEFATYIEQVIKNTQAMADEFAKVDGLRLIAGGSDNHLLN
LKVLDLGINGKEAQDLLDSVHITLNKEAIPDETLSPFKTSGVRIGAAAITSRGFKEAEARKVAQLVSKALVNHDNQEKLE
EVRKSALELTHQFPL
>Mature_415_residues
MIFDKEDFESFDPELWAAIHAEEIRQQQNIELIASENIVSKAVMAAQGSVLTNKYAEGYPGKRYYGGTEAVDVVENLAID
RAKELFGAKFANVQPHSGSQANAAAYMALIQPGDTVLGMDLNAGGHLTHGASVNFSGKTYHFVPYGVNPQTELLDYEEIL
KIAKEVQPKLIVAGASAYSRLIDFAKFRQIADSVGAKLMVDMAHIAGLVATGAHPNPLPYADVVTTTTHKTLRGPRGGMI
LTNDEVLAKKINSAIFPGTQGGPLEHVIAAKAVAFKEALDPEFATYIEQVIKNTQAMADEFAKVDGLRLIAGGSDNHLLN
LKVLDLGINGKEAQDLLDSVHITLNKEAIPDETLSPFKTSGVRIGAAAITSRGFKEAEARKVAQLVSKALVNHDNQEKLE
EVRKSALELTHQFPL

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=409, Percent_Identity=45.721271393643, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI261862350, Length=409, Percent_Identity=45.721271393643, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI261862348, Length=409, Percent_Identity=45.721271393643, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI19923315, Length=409, Percent_Identity=45.721271393643, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI261862346, Length=409, Percent_Identity=44.4987775061125, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI22547186, Length=402, Percent_Identity=44.2786069651741, Blast_Score=316, Evalue=2e-86,
Organism=Homo sapiens, GI22547189, Length=388, Percent_Identity=41.7525773195876, Blast_Score=283, Evalue=3e-76,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=57.5539568345324, Blast_Score=472, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI25144732, Length=404, Percent_Identity=46.039603960396, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI25144729, Length=404, Percent_Identity=46.039603960396, Blast_Score=352, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6319739, Length=416, Percent_Identity=44.4711538461538, Blast_Score=351, Evalue=1e-97,
Organism=Saccharomyces cerevisiae, GI6323087, Length=402, Percent_Identity=45.273631840796, Blast_Score=334, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24640005, Length=449, Percent_Identity=42.3162583518931, Blast_Score=357, Evalue=9e-99,
Organism=Drosophila melanogaster, GI221329721, Length=449, Percent_Identity=42.3162583518931, Blast_Score=356, Evalue=2e-98,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 44823; Mature: 44823

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFDKEDFESFDPELWAAIHAEEIRQQQNIELIASENIVSKAVMAAQGSVLTNKYAEGYP
CCCCCCHHHHCCHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCHHHHHHHCCCC
GKRYYGGTEAVDVVENLAIDRAKELFGAKFANVQPHSGSQANAAAYMALIQPGDTVLGMD
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
LNAGGHLTHGASVNFSGKTYHFVPYGVNPQTELLDYEEILKIAKEVQPKLIVAGASAYSR
ECCCCCEECCCEEEECCCEEEEEECCCCCCHHHCCHHHHHHHHHHCCCEEEEECHHHHHH
LIDFAKFRQIADSVGAKLMVDMAHIAGLVATGAHPNPLPYADVVTTTTHKTLRGPRGGMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEECCCHHHHCCCCCCEE
LTNDEVLAKKINSAIFPGTQGGPLEHVIAAKAVAFKEALDPEFATYIEQVIKNTQAMADE
EECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FAKVDGLRLIAGGSDNHLLNLKVLDLGINGKEAQDLLDSVHITLNKEAIPDETLSPFKTS
HHHCCCEEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHEEEECCCCCCCCCCCCCHHC
GVRIGAAAITSRGFKEAEARKVAQLVSKALVNHDNQEKLEEVRKSALELTHQFPL
CEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIFDKEDFESFDPELWAAIHAEEIRQQQNIELIASENIVSKAVMAAQGSVLTNKYAEGYP
CCCCCCHHHHCCHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCHHHHHHHCCCC
GKRYYGGTEAVDVVENLAIDRAKELFGAKFANVQPHSGSQANAAAYMALIQPGDTVLGMD
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
LNAGGHLTHGASVNFSGKTYHFVPYGVNPQTELLDYEEILKIAKEVQPKLIVAGASAYSR
ECCCCCEECCCEEEECCCEEEEEECCCCCCHHHCCHHHHHHHHHHCCCEEEEECHHHHHH
LIDFAKFRQIADSVGAKLMVDMAHIAGLVATGAHPNPLPYADVVTTTTHKTLRGPRGGMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEECCCHHHHCCCCCCEE
LTNDEVLAKKINSAIFPGTQGGPLEHVIAAKAVAFKEALDPEFATYIEQVIKNTQAMADE
EECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FAKVDGLRLIAGGSDNHLLNLKVLDLGINGKEAQDLLDSVHITLNKEAIPDETLSPFKTS
HHHCCCEEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHEEEECCCCCCCCCCCCCHHC
GVRIGAAAITSRGFKEAEARKVAQLVSKALVNHDNQEKLEEVRKSALELTHQFPL
CEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]