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The map label for this gene is cobS [H]

Identifier: 124486259

GI number: 124486259

Start: 1475289

End: 1476029

Strand: Reverse

Name: cobS [H]

Synonym: Mlab_1443

Alternate gene names: 124486259

Gene position: 1476029-1475289 (Counterclockwise)

Preceding gene: 124486263

Following gene: 124486257

Centisome position: 81.78

GC content: 53.85

Gene sequence:

>741_bases
ATGCAGTCACTTCGCGCACTCTTACAGTTCACCACGATCCTCCCTCTGGGGAAACCGGCGGATTATGAATGTTTTGCGAA
GCGAAGCTGGCTCTATCCGATAGCAGGATACGTCACCGGCGGTATCGCGGCACTTCCCGGGCTGATCGCCTGGTATCTCG
GATTTGAAAACTCTTCGATAATCGCCGCCCTCACCCTTGCCTTGGCGGTCTTCATTTCCGGCGGCAACCACTTCGACGGA
CTTCTCGACTTCGGCGACGGACTCATGGCGCATGGAAGCAGGGAAAAACGGATACGTGCCATGACCGACCGAACGACAGG
TGCCGGGGCACTCGCTCTTGGAATCATGGTTGTTCTGATTTCCTACGCCGCTCTCTCATCCATGACCGTCCTGCAAATCG
CTTTCGCCGTGTTCATAGCAGAAATTTTTGGGAAACTGAGTATGGCTTTCTCATCAGCGCTTGGAAAACCATTTCACGAC
GGGATTCAGAAATATATCTACGATCGTTCAAAAAAGCGGTTCTGCCTCTATGCCGTACTCTTAACTCTCCCGCTCTTCCT
TTTGCCTGGAAAAATGTATGTCGGGGCAGGACTCGCTGCGGCTCTTGTCACATTCCTGTGCCTCCATTTCCTCGCGCGAA
AACTCTTCGGCGGAACGAACGGGGATGTGACCGGTGCGGGCAACGAAATCACCCGGATGGCCGTTCTTCTGGTCGTAGCG
GTAATACTAACGTCTGCGTGA

Upstream 100 bases:

>100_bases
AGAATCAGGGCGAACATCGGAGACTCTGCAGAGAAATCCGCTCTCGTTGAAACAAATGACATGATATTATATCATTTAGG
ACTGACAAGATATAATATAA

Downstream 100 bases:

>100_bases
GAGCGGCTGGTATCCGGACCCTGCGTCATCCGAACACTCGATATCGGTGGCATTCTTGTCTTCGATCACGGTGTTGAAAA
GGGTCATTCCTTCCATTAAC

Product: cobalamin-5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MQSLRALLQFTTILPLGKPADYECFAKRSWLYPIAGYVTGGIAALPGLIAWYLGFENSSIIAALTLALAVFISGGNHFDG
LLDFGDGLMAHGSREKRIRAMTDRTTGAGALALGIMVVLISYAALSSMTVLQIAFAVFIAEIFGKLSMAFSSALGKPFHD
GIQKYIYDRSKKRFCLYAVLLTLPLFLLPGKMYVGAGLAAALVTFLCLHFLARKLFGGTNGDVTGAGNEITRMAVLLVVA
VILTSA

Sequences:

>Translated_246_residues
MQSLRALLQFTTILPLGKPADYECFAKRSWLYPIAGYVTGGIAALPGLIAWYLGFENSSIIAALTLALAVFISGGNHFDG
LLDFGDGLMAHGSREKRIRAMTDRTTGAGALALGIMVVLISYAALSSMTVLQIAFAVFIAEIFGKLSMAFSSALGKPFHD
GIQKYIYDRSKKRFCLYAVLLTLPLFLLPGKMYVGAGLAAALVTFLCLHFLARKLFGGTNGDVTGAGNEITRMAVLLVVA
VILTSA
>Mature_246_residues
MQSLRALLQFTTILPLGKPADYECFAKRSWLYPIAGYVTGGIAALPGLIAWYLGFENSSIIAALTLALAVFISGGNHFDG
LLDFGDGLMAHGSREKRIRAMTDRTTGAGALALGIMVVLISYAALSSMTVLQIAFAVFIAEIFGKLSMAFSSALGKPFHD
GIQKYIYDRSKKRFCLYAVLLTLPLFLLPGKMYVGAGLAAALVTFLCLHFLARKLFGGTNGDVTGAGNEITRMAVLLVVA
VILTSA

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 26256; Mature: 26256

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCC
>Mature Secondary Structure
MQSLRALLQFTTILPLGKPADYECFAKRSWLYPIAGYVTGGIAALPGLIAWYLGFENSSI
CHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
IAALTLALAVFISGGNHFDGLLDFGDGLMAHGSREKRIRAMTDRTTGAGALALGIMVVLI
HHHHHHHHHHHHCCCCCCCHHHHHCCCHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
SYAALSSMTVLQIAFAVFIAEIFGKLSMAFSSALGKPFHDGIQKYIYDRSKKRFCLYAVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHH
LTLPLFLLPGKMYVGAGLAAALVTFLCLHFLARKLFGGTNGDVTGAGNEITRMAVLLVVA
HHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
VILTSA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA